A Rapid and Economical Method for Low Molecular Weight RNA Isolation from a Wide Variety of Plant Species

2013 ◽  
Vol 77 (7) ◽  
pp. 1599-1601 ◽  
Author(s):  
Zewei AN ◽  
Yachao LI ◽  
Lili XIE ◽  
Qilin ZHAI ◽  
Huasun HUANG
Keyword(s):  
Molecular Weight ◽  
Plant Species ◽  
Rna Isolation ◽  
Low Molecular Weight ◽  
Economical Method

scholarly journals The major low-molecular-weight heat shock protein in chloroplasts shows antigenic conservation among diverse higher plant species.

1989 ◽  
Vol 9 (2) ◽  
pp. 461-468 ◽  
Author(s):  
E Vierling ◽  
L M Harris ◽  
Q Chen
Keyword(s):  
Molecular Weight ◽  
Heat Shock ◽  
Plant Species ◽  
Fusion Proteins ◽  
Higher Plants ◽  
Low Molecular Weight ◽  
Amino Terminal ◽  
Terminal Domain ◽  
Wide Range ◽  
Carboxyl Terminal

Several plant species are known to synthesize low-molecular-weight nucleus-encoded heat shock proteins (HSPs) which localize to chloroplasts. DNA sequence analysis of chloroplast HSP cDNAs from pea (Pisum sativum) and soybean (Glycine max) has shown that the carboxyl-terminal halves of these proteins are homologous to low-molecular-weight HSPs from a wide range of eucaryotes (E. Vierling, R. T. Nagao, A. E. DeRocher, and L. M. Harris, EMBO J. 7:575-581, 1988). We used a pea cDNA to construct fusion proteins containing either the carboxyl-terminal heat shock domain or the amino-terminal domain of the chloroplast HSP. The fusion proteins were overexpressed in Escherichia coli and used to produce choloroplast HSP-specific polyclonal antibodies. The carboxyl-terminal antibodies recognized chloroplast HSP precursor proteins from pea and from three divergent plant species, Arabidopsis thaliana, petunia (Petunia hybrida), and maize (Zea mays). The amino-terminal antibodies recognized effectively only the pea precursor. When intact plants of each species were subjected to a heat stress regime mimicking field growth conditions, significant levels of the mature forms of the chloroplast HSPs accumulated in pea, A. thaliana, and maize. The levels of accumulated HSPs remained unchanged for 12 h following the stress treatment. We conclude that the synthesis of chloroplast-localized HSPs is an important component of the stree response in all higher plants and that chloroplast HSPs from dicotyledonous and monocotyledonous plants have a conserved carboxyl-terminal domain.

The Plant Toxin Victorin Binds to a Discrete Number of Proteins in All Organisms Tested

1994 ◽  
Vol 21 (2) ◽  
pp. 243 ◽  
Author(s):  
DC Loschke ◽  
LD Tomaska ◽  
H Chen ◽  
DW Gabriel ◽  
BG Rolfe
Keyword(s):  
Molecular Weight ◽  
Plant Species ◽  
Binding Proteins ◽  
Low Molecular Weight ◽  
In Vitro Experiments ◽  
Plant Toxin ◽  
Binding Properties ◽  
Saprophytic Fungus ◽  
Biological Probe

We have used the plant toxin victorin C, which is synthesised by the saprophytic fungus Cochliobolus victoria, as a biological probe. Victorin C, labelled with either 125I or 35S, bound to eight distinct proteins (victorin-binding proteins) in oat plants that were either resistant or sensitive to the toxin. Using a series of in vitro experiments, we observed a difference in the victorin-binding properties between resistant and susceptible plants. Furthermore, we found that other plant species contain a set of proteins of similar sizes which specifically bind victorin. Unexpectedly, we also found a low molecular weight victorin-binding protein present in all tested eukaryotic and prokaryotic cells.

The major low-molecular-weight heat shock protein in chloroplasts shows antigenic conservation among diverse higher plant species

1989 ◽  
Vol 9 (2) ◽  
pp. 461-468
Author(s):  
E Vierling ◽  
L M Harris ◽  
Q Chen
Keyword(s):  
Molecular Weight ◽  
Heat Shock ◽  
Plant Species ◽  
Fusion Proteins ◽  
Higher Plants ◽  
Low Molecular Weight ◽  
Amino Terminal ◽  
Terminal Domain ◽  
Wide Range ◽  
Carboxyl Terminal

Several plant species are known to synthesize low-molecular-weight nucleus-encoded heat shock proteins (HSPs) which localize to chloroplasts. DNA sequence analysis of chloroplast HSP cDNAs from pea (Pisum sativum) and soybean (Glycine max) has shown that the carboxyl-terminal halves of these proteins are homologous to low-molecular-weight HSPs from a wide range of eucaryotes (E. Vierling, R. T. Nagao, A. E. DeRocher, and L. M. Harris, EMBO J. 7:575-581, 1988). We used a pea cDNA to construct fusion proteins containing either the carboxyl-terminal heat shock domain or the amino-terminal domain of the chloroplast HSP. The fusion proteins were overexpressed in Escherichia coli and used to produce choloroplast HSP-specific polyclonal antibodies. The carboxyl-terminal antibodies recognized chloroplast HSP precursor proteins from pea and from three divergent plant species, Arabidopsis thaliana, petunia (Petunia hybrida), and maize (Zea mays). The amino-terminal antibodies recognized effectively only the pea precursor. When intact plants of each species were subjected to a heat stress regime mimicking field growth conditions, significant levels of the mature forms of the chloroplast HSPs accumulated in pea, A. thaliana, and maize. The levels of accumulated HSPs remained unchanged for 12 h following the stress treatment. We conclude that the synthesis of chloroplast-localized HSPs is an important component of the stree response in all higher plants and that chloroplast HSPs from dicotyledonous and monocotyledonous plants have a conserved carboxyl-terminal domain.

Phytoextraction of cadmium-contaminated soils: comparison of plant species and low molecular weight organic acids

2019 ◽  
Vol 22 (4) ◽  
pp. 383-391 ◽  
Author(s):  
Guo Yu ◽  
Jie Liu ◽  
Yumei Long ◽  
Zhe Chen ◽  
Geoffrey I. Sunahara ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Organic Acids ◽  
Plant Species ◽  
Contaminated Soils ◽  
Low Molecular Weight

A high-performance oil-free backing pump for electron microscopes

1978 ◽  
Vol 36 (1) ◽  
pp. 110-111
Author(s):  
G.K.W. Balkau ◽  
E. Bez ◽  
J.L. Farrant
Keyword(s):  
Molecular Weight ◽  
Electron Microscope ◽  
Hot Spots ◽  
High Performance ◽  
Carbonaceous Material ◽  
Contamination Rate ◽  
Low Molecular Weight ◽  
Principal Source ◽  
Rotary Pumps ◽  
Electron Microscopes

The earliest account of the contamination of electron microscope specimens by the deposition of carbonaceous material during electron irradiation was published in 1947 by Watson who was then working in Canada. It was soon established that this carbonaceous material is formed from organic vapours, and it is now recognized that the principal source is the oil-sealed rotary pumps which provide the backing vacuum. It has been shown that the organic vapours consist of low molecular weight fragments of oil molecules which have been degraded at hot spots produced by friction between the vanes and the surfaces on which they slide. As satisfactory oil-free pumps are unavailable, it is standard electron microscope practice to reduce the partial pressure of organic vapours in the microscope in the vicinity of the specimen by using liquid-nitrogen cooled anti-contamination devices. Traps of this type are sufficient to reduce the contamination rate to about 0.1 Å per min, which is tolerable for many investigations.

Low-molecular-weight heparin in the prevention and treatment of thromboembolic disorders

1998 ◽  
Vol 1 (5) ◽  
pp. 166-174 ◽  
Author(s):  
Evelyn R Hermes De Santis ◽  
Betsy S Laumeister ◽  
Vidhu Bansal ◽  
Vandana Kataria ◽  
Preeti Loomba ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight Heparin ◽  
Low Molecular Weight ◽  
Prevention And Treatment

Venous thromboembolism in a general hospital. An update with low molecular weight heparin prophylaxis

VASA ◽  
2007 ◽  
Vol 36 (1) ◽  
pp. 17-22
Author(s):  
Schulz ◽  
Kesselring ◽  
Seeberger ◽  
Andresen
Keyword(s):  
Molecular Weight ◽  
Pulmonary Embolism ◽  
Venous Thromboembolism ◽  
Low Molecular Weight ◽  
Trauma Patients ◽  
Vte Prophylaxis ◽  
Vein Thrombosis ◽  
Patients At Risk ◽  
Hospital Stays ◽  
Event Rates

Background: Patients admitted to hospital for surgery or acute medical illnesses have a high risk for venous thromboembolism (VTE). Today’s widespread use of low molecular weight heparins (LMWH) for VTE prophylaxis is supposed to have reduced VTE rates substantially. However, data concerning the overall effectiveness of LMWH prophylaxis is sparse. Patients and methods: We prospectively studied all patients with symptomatic and objectively confirmed VTE seen in our hospital over a three year period. Event rates in different wards were analysed and compared. VTE prophylaxis with Enoxaparin was given to all patients at risk during their hospital stay. Results: A total of 50 464 inpatients were treated during the study period. 461 examinations were carried out for symptoms suggestive of VTE and yielded 89 positive results in 85 patients. Seventy eight patients were found to have deep vein thrombosis, 7 had pulmonary embolism, and 4 had both deep venous thrombosis and pulmonary embolism. The overall in hospital VTE event rate was 0.17%. The rate decreased during the study period from 0.22 in year one to 0,16 in year two and 0.13 % in year three. It ranged highest in neurologic and trauma patients (0.32%) and lowest (0.08%) in gynecology-obstetrics. Conclusions: With a simple and strictly applied regimen of prophylaxis with LMWH the overall rate of symptomatic VTE was very low in our hospitalized patients. Beside LMWH prophylaxis, shortening hospital stays and substantial improvements in surgical and anasthesia techniques achieved during the last decades probably play an essential role in decreasing VTE rates.

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