scholarly journals Isolation and Screening of Water Microbes for Decolourisation of Textile Dye Waste

2016 ◽  
Vol 11 (1) ◽  
pp. 296-300
Author(s):  
J. K Singh ◽  
R Ranjan ◽  
Pranay Pankaj
Keyword(s):  
Waste Water ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Azo Dyes ◽  
Bacterial Species ◽  
Textile Dye ◽  
Human Beings ◽  
Degrading Bacteria ◽  
Vis Spectroscopy ◽  
Pre Treatment

Azo dyes are widely used in textile industry. Unused dyes, consisting mainly non biodegradable released along with waste water streams without any proper pre-treatment which cause nuisance for environment and accumulate in flora as well as fauna. These also exhibit allergic, carcinogenic and mutagenic properties for human beings. Isolation and screening of azo dye degrading bacteria are economic in biodegradation and detoxification. In the present study, 200 waste water samples were collected from dye-contaminated sites of textile industries and bacterial species such as Bacillus subtilis, Pseudomonas aeruginosa and Psuedomonas putida were isolated and identified. Evaluation of decolorizing properties of these bacteriae were done by UV-Vis spectroscopy (Amax 596 nm) in different concentrations using different carbon sources such as Hans’s medium and GYP medium. Maximum decolourisation of 0.1% azo dyes were recorded to be 89.0%, 91% and 86% in Hans medium containing charcoal source by Bacillus subtilis, Pseudomonas aeruginosa and Psuedomonas putida respectively at 24 hrs. These bacterial isolates may be utilized in large scale for pre-treatment for ecological balance by avoiding water pollution.

scholarly journals Retarded swarming motility in Bacillus subtilis NRS-762 and Pseudomonas aeruginosa PRD-10

2018 ◽  
Author(s):  
Wenfa Ng
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Bacterial Species ◽  
Nutritional Stress ◽  
Cellular Level ◽  
Bacterial Cells ◽  
Loss Of Function ◽  
Swarming Motility ◽  
Bacteria Growth ◽  
Solid Media

Coping with nutritional stress is essential for cell survival, of which many strategies at the cellular level lend support for ensuring the survival of the population at a particular habitat. One postulated mechanism is swarming motility in bacterial cells, where, upon depletion of nutrients at a locale, cells would coordinate their movement, synthesize more flagella, and secrete lubricants for moving rapidly across surfaces in search for food. Known to engage in swarming motility, Bacillus subtilis and Pseudomonas aeruginosa are two common bacterial species with versatile metabolism that use the motility mode to colonize new habitats with more favourable environmental and nutritional conditions. However, experimental observations of bacteria growth on a variety of agar media revealed that B. subtilis NRS-762 (ATCC 8473) and P. aeruginosa PRD-10 (ATCC 15442) exhibited retarded swarming motility upon entry into stationary phase on solid media. Specifically, B. subtilis NRS-762 colonies exhibited round, wrinkled morphologies compared to complex filamented swarming patterns common in strains able to engage in swarming motility. On the other hand, P. aeruginosa PRD-10 colonies were round, mucoid, and expanded outwards from the colony centre without extending filaments from the centre; thereby, indicating retarded swarming motility. Thus, impaired cellular machinery for swarming motility or mutated and deleted genes likely account for observed retarded swarming motility in B. subtilis NRS-762 and P. aeruginosa PRD-10. More importantly, observations of small filaments extending radially from an expanded colony of P. aeruginosa PRD-10 grown on minimal salts medium supplemented with yeast extract highlighted possible loss of function of effector molecules that transmit cellular decision at swarming motility into movement, while sensory mechanisms feeding into the motility mechanism remained intact. More broadly, observations of impaired swarming motility in B. subtilis NRS-762 and P. aeruginosa PRD-10 in two species otherwise endowed with the motility mode highlighted that additional triggers for swarming motility are likely present, and the motility mode may have been evolutionary selected for other functions in addition to foraging for food in times of nutritional stress.

Adaptive and cross resistance to cadmium (II) and zinc (II) by Pseudomonas aeruginosa BC15

Biologia ◽  
2008 ◽  
Vol 63 (4) ◽  
Author(s):  
Chelliah Edward Raja ◽  
Sundaresan Sasikumar ◽  
Govindan Selvam
Keyword(s):  
Waste Water ◽  
Pseudomonas Aeruginosa ◽  
Metal Ion ◽  
Binary Solution ◽  
Polluted Water ◽  
Cross Resistance ◽  
Human Beings ◽  
Adaptive Resistance ◽  
Essential Nutrient ◽  
Hazardous Metal

AbstractCadmium and zinc appear in the combined forms and they are co-pollutants. Cd is the most hazardous metal ion for human beings and causes renal dysfunction, liver and lungs damage, bone degeneration and blood damage. Though Zn is an essential nutrient, excess of Zn is toxic. Biological process was more important because conventional methods fail to remediate these pollutants due to high costs and less affordability. The screening and understanding of the functioning of microorganism plays an important role in removal and recovery of metals from heavy-metal-polluted water and soil. In our study, the strain Pseudomonas aeruginosa BC15 was isolated from oil-mill-treated waste water and it showed to be highly resistant to 6 mM Cd and 20 mM Zn in the solid and liquid media. The growth studies of BC15 strain in the medium without induction exhibited high tolerable capacity when compared to other microbes. Pretreatment of P. aeruginosa BC15 with sub-lethal concentrations of Cd induced adaptive resistance to lethal doses of Cd. Cadmium-induced cells also showed cross resistance to lethal concentration of zinc. The organism had high resistance against Cd and Zn. This has been clearly proven through biosorption studies: Cd was absorbed up to 62% and Zn about 60% in single solution, whereas in binary solution Cd was biosorbed up to 82% and Zn 85%. In conclusion, this study reveals the significance of using the strain P. aeruginosa BC15 in the bioremediation of Cd and Zn from industrial waste water and contaminated soil.

scholarly journals Assessment of anti bacterial screening of Pongamia pinnata stem against bacterial species: An In-vitro approach

2019 ◽  
Vol 9 (1-s) ◽  
pp. 181-184
Author(s):  
Adithya Gadeela Manish ◽  
Nerella Mounika ◽  
Bakshi Vasudha ◽  
Boggula Narender
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Medicinal Plants ◽  
Bacterial Species ◽  
Bacterial Activity ◽  
Pongamia Pinnata ◽  
Bacterial Strains ◽  
Agar Diffusion ◽  
Diffusion Technique

Numerous studies have shown that aromatic and medicinal plants are sources of diverse nutrient and non-nutrient molecules which protect the human body against various pathogens. Nature has been a source of medicinal agents for thousands of years and a large number of modern drugs have been isolated from natural sources. Herbal medicine is the oldest known healthcare system known to mankind. India has rich medicinal plants of nearly 7500 species. Many medicinal plants were with a long history of use in folk medicine against a variety of diseases. Recently, many researchers have taken a great interest on medicinal plants for their phytochemical constituents and biological activities including anti microbial activity. The anti bacterial activity of the ethanolic crude stem extract of Pongamia pinnata against four bacterial species (Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and Escherichia coli) was investigated, using agar diffusion technique. At concentrations ranging from 10-40 mg/mL, the ethanolic crude extract showed activity against the four bacteria (Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and E. coli) from 2 mm-20 mm, after 24 hours incubation. The present study showed the effectiveness of the crude plant extract against the tested bacterial strains and indicates the potential use of the extract as anti bacterial agent for the control of infectious diseases. Keywords:  Pongamia pinnata, anti bacterial activity, agar diffusion technique, bacterial strains, chloramphenicol.

Isolation and Characterization of Hydrocarbon-degrading Bacteria in Soils of Mechanical Workshops in Maiduguri, Borno State

2021 ◽  
Vol 4 (2) ◽  
pp. 35-38
Author(s):  
Hussaini Shettima ◽  
Ibrahim Alkali Allamin ◽  
Nasir Halima ◽  
Haruna Yahya Ismail ◽  
Yusuf Musa
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Licheniformis ◽  
Heterotrophic Bacteria ◽  
Bacterial Species ◽  
Bacillus Coagulans ◽  
Engine Oil ◽  
Klebsiella Pneumonia ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Hydrocarbon Utilizing Bacteria

The study was conducted to determine the distribution of hydrocarbon utilizing bacteria in spent engine oil (SEO) contaminated soil. Five mechanical workshops within Maiduguri Metropolis. Five bulk soil sample comprising of one each of the five sites; the sites are Leventis Area on Kashim Ibrahim Way, Damboa Raod, Boiler (commonly called Bola) near Maiduguri Monday Market, Ngomari, on Kano Road, and 1000 Housing Estate, also on Kano Road. Nutrient agar was used, or isolation and enumeration total heterotrophic bacteria and Mineral salt agar was used for the isolation and enumeration of hydrocarbon utilizing bacteria. The result shows THB (92.0x104) at Damboa road (DR) to as higher and (78.8x104) at Bola area (BL) while HUB (2.0x104) at Damboa road (DR) and as high as (9.3x104) at Bola area (BL). The bacterial species isolated were species of Bacillus licheniformis, Bacillus subtilis, Bacillus coagulans, Bacillus alvei, Bacillus cereus, Bacillus lentus other are Pseudomonas aeruginosa, Klebsiella pneumonia. Bacillus licheniformis, and Bacillus subtilis are the most occurring bacterial isolates identified. The ability of those bacterial isolate to degrade hydrocarbon buoyantly will help in remediation of oil polluted environments.

scholarly journals Biodegradation potential of bacterial isolates from dye wastewater at Marina, Sokoto metropolis

2020 ◽  
Vol 36 (2) ◽  
pp. 87-94
Author(s):  
M.A. Adegbite ◽  
K. Ibrahim ◽  
S.A. Yusif
Keyword(s):  
Waste Water ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Bacterial Species ◽  
Waste Water Treatment ◽  
Dye Wastewater ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Promising Tool ◽  
Degradation Ability

This study was carried out to determine the biodegradation potential of bacterial isolates from dye wastewater at Marina Area, Sokoto. Three (3) of the bacteria were identified; Bacillus subtilis, Luteimonasaestuarrii and Bacillus cereus and were used for further studies based on their degradation ability of green, red, yellow and blue dyes. Bacillus subtilis, Luteimonas aestuarii and Bacillus cereus have the potential to degrade all the four dyes used during the present investigation. However, efficacy of the various bacterial strains was found to vary at different concentration levels in degradation of a particular dye. Thus, by this study it can be concluded that Bacillus cereus, Bacillus subtilis and Luteimonas aestuarii can be used as a good microbial source for dye waste water treatment. The selected bacterial species represent a promising tool for application in biodegradation of dye waste water and the potential observed would allow for the application of the bacterial isolates for treatment of dye effluents before disposal. Keywords: effluents, isolates, biodegradation, dye and bacterial.

scholarly journals Antibacterial and antibiofilm properties of Phlomis and Stachys species

2020 ◽  
Vol 49 (2) ◽  
pp. 257-263
Author(s):  
Aynur Aybey
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Ethyl Acetate ◽  
Bacterial Species ◽  
Bacterial Strains ◽  
Methanol Extracts ◽  
Good Antibacterial Activity ◽  
Antibiofilm Agents

The ethyl acetate and methanol extracts of Phlomis pungens var. pungens, P. nissolii, P. armeniaca and Stachys byzantina, S. cretica L. subsp. mersiaea and S. cretica L. subsp. Smyrnaea were evaluated for their antibacterial and antibiofilm activities. Five bacterial species were used are Bacillus subtilis, Pseudomonas aeruginosa, Shigella sonnei, Salmonella typhimurium and Yersinia enterocolitica. The ethyl acetate and methanol extracts of plant species showed good antibacterial activity against all bacterial strains. More significantly, ethylacetate and methanol extracts of all Phlomis species were found to be more effective on degredation of mature biofilm against all used bacterial strains than extracts of Stachys species. The findings of the present study highlight the promising role of Phlomis and Stachys extracts as new lead structures in the search for novel antibacterial and antibiofilm agents.

scholarly journals Bacterial Biodegradation of Crude Oil Using Local Isolates

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Raed S. Al-Wasify ◽  
Shimaa R. Hamed
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Crude Oil ◽  
Oil Spills ◽  
Bacterial Species ◽  
Bacterial Consortium ◽  
Contaminated Water ◽  
Water And Soil Samples ◽  
Bacterial Biodegradation ◽  
Mixed Bacterial Consortium

An experimental study was undertaken to assess the efficiency of Pseudomonas aeruginosa, Bacillus subtilis, and Acinetobacter lwoffi isolated from petroleum contaminated water and soil samples to degrade crude oil, separately and in a mixed bacterial consortium. Capillary gas chromatography was used for testing the effect of those bacterial species on the biodegradation of crude oil. Individual bacterial cultures showed less growth and degradation than did the mixed bacterial consortium. At temperature 22°C, the mixed bacterial consortium degraded a maximum of 88.5% of Egyptian crude oil after 28 days of incubation. This was followed by 77.8% by Pseudomonas aeruginosa, 76.7% by Bacillus subtilis, and 74.3% by Acinetobacter lwoffi. The results demonstrated that the selected bacterial isolates could be effective in biodegradation of oil spills individually and showed better biodegradation abilities when they are used together in mixed consortium.

scholarly journals Toxicity Assessment of Biologically Degraded Product of Textile Dye Acid Red G

2019 ◽  
Vol 4 (4) ◽  
pp. 236-243
Author(s):  
Priti Faldu ◽  
Vishal Kothari ◽  
Charmy Kothari ◽  
Jalpa Rank ◽  
Ankit Hinsu ◽  
...  
Keyword(s):  
Azo Dyes ◽  
Environmental Concern ◽  
Bacterial Species ◽  
Amino Nitrogen ◽  
Positive Control ◽  
Toxicity Assessment ◽  
Textile Dye ◽  
Dye Pollutants ◽  
Chromosome Aberration Assay ◽  
Acid Red G

Azo dyes are of environmental concern due to their recalcitrant nature. Several azo dyes and their decolorized and degraded products exert toxic and mutagenic effects on the flora and fauna. The toxic properties of these azo dyes are due to nature and position of the substitution with respect to the aromatic rings and amino nitrogen atoms. Several studies have thus far been emphasized on biodegradation of azo dye pollutants, though role of their biodegraded product is rarely studied. Given a lack of this understanding, we have analyzed the effects of degraded products of a di-azo textile dye Acid Red G by newly isolated bacterial species, Pseudomonas aeruginosa PFK10 and Brevibacillus choshinensis PFK11. The genotoxicity and cytotoxicity of Acid Red G and their degraded products were tested on HeLa cell line and Human lymphocyte cell, respectively. The data of MTT assay has been shown that activity of degraded products of the Acid Red G were comparable to their parent dye. But chromosome aberration assay and sister chromatid exchange assay did not show any significant changes in chromosomes as compared to positive control mitomicine.

scholarly journals Retarded swarming motility in Bacillus subtilis NRS-762 and Pseudomonas aeruginosa PRD-10

2018 ◽  
Author(s):  
Wenfa Ng
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Bacterial Species ◽  
Nutritional Stress ◽  
Cellular Level ◽  
Bacterial Cells ◽  
Loss Of Function ◽  
Swarming Motility ◽  
Bacteria Growth ◽  
Solid Media

Coping with nutritional stress is essential for cell survival, of which many strategies at the cellular level lend support for ensuring the survival of the population at a particular habitat. One postulated mechanism is swarming motility in bacterial cells, where, upon depletion of nutrients at a locale, cells would coordinate their movement, synthesize more flagella, and secrete lubricants for moving rapidly across surfaces in search for food. Known to engage in swarming motility, Bacillus subtilis and Pseudomonas aeruginosa are two common bacterial species with versatile metabolism that use the motility mode to colonize new habitats with more favourable environmental and nutritional conditions. However, experimental observations of bacteria growth on a variety of agar media revealed that B. subtilis NRS-762 (ATCC 8473) and P. aeruginosa PRD-10 (ATCC 15442) exhibited retarded swarming motility upon entry into stationary phase on solid media. Specifically, B. subtilis NRS-762 colonies exhibited round, wrinkled morphologies compared to complex filamented swarming patterns common in strains able to engage in swarming motility. On the other hand, P. aeruginosa PRD-10 colonies were round, mucoid, and expanded outwards from the colony centre without extending filaments from the centre; thereby, indicating retarded swarming motility. Thus, impaired cellular machinery for swarming motility or mutated and deleted genes likely account for observed retarded swarming motility in B. subtilis NRS-762 and P. aeruginosa PRD-10. More importantly, observations of small filaments extending radially from an expanded colony of P. aeruginosa PRD-10 grown on minimal salts medium supplemented with yeast extract highlighted possible loss of function of effector molecules that transmit cellular decision at swarming motility into movement, while sensory mechanisms feeding into the motility mechanism remained intact. More broadly, observations of impaired swarming motility in B. subtilis NRS-762 and P. aeruginosa PRD-10 in two species otherwise endowed with the motility mode highlighted that additional triggers for swarming motility are likely present, and the motility mode may have been evolutionary selected for other functions in addition to foraging for food in times of nutritional stress.

scholarly journals Phenolic Content and Antimicrobial activities of Date Palm (Phoenix dactylifera L.) Fruits and Leaves

Food Biology ◽  
2017 ◽  
Vol 6 ◽  
pp. 11 ◽  
Author(s):  
Hajira A. Qadoos ◽  
Hend S. Dhafari ◽  
Dalal A. Al Marzooqi ◽  
Al yzyah I. Yaqoubi ◽  
Alagappan Kumarappan ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Date Palm ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Bacterial Species ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Palm Fruit

<p>This study aimed to investigate total phenolic content (TPC) and antibacterial activities of different extracts recovered from Date Palm fruit and leaves using various solvents, including methanol, ethanol and water. The results showed that the highest phenolic content was found in Leaf Methanol extract, followed by Leaf Ethanol extract, and then followed by Seed Methanol extract (74.4 mg/g, 67.3 mg/g, and 64.7 mg/g respectively). Whereas, the date methanol extract had the lowest phenolic content (5 mg/g). The study was also scrutinized to find the antibacterial inhibitory property in the leaves and fruits of date palm against four bacterial species (<em>E.coli</em>, <em>Staphylococcus aureus</em>, <em>Bacillus subtilis</em>, and <em>Pseudomonas aeruginosa</em>) by using the well diffusion method and minimum inhibitory concentration (MIC). Date leaves extracts showed inhibitory effect on the growth of <em>Staphylococcus aureu</em>s and <em>Bacillus subtilis</em> and resistance to <em>Pseudomonas aeruginosa</em> and <em>E.coli</em>. MIC of methanol and ethanol (70%) leaves extract were 100 mg/ml and 250mg/ml for <em>S. aureus</em> and <em>B. subtilis</em> respectively, however, all the bacteria have resistance to date fruits extracts.</p>

Sign in / Sign up

Export Citation Format

Share Document