Estimation of Levels of Glutathione Peroxidase (Gpx), Malondialdehyde (Mda), Tumor Necrosis Factor Alpha (Tnf Alpha) and Alpha Feto Protein (Afp) In Saliva of Potentially Malignant Disorders and Oral Squamous Cell Carcinoma.

The aim of the study was to estimate the levels of Glutathione peroxidase (GPx), malondialdehyde (MDA), Tumor necrosis factor alpha (TNF alpha) and Alpha feto protein (AFP) in saliva of potentially malignant disorders and oral squamous cell carcinoma, use them as an effective biomarkers in screening and diagnosis of oral squamous cell carcinoma as it is less invasive and more economical. 30 newly diagnosed patients with oral sub mucous fibrosis, oral leukoplakia, oral squamous cell carcinoma, who were not previously treated for the disease was selected for the study. 5ml of unstimulated saliva was collected from the patient for one minute in a sterile URICOL container and stored in sub-zero temperature before processing of the samples. Glutathione, malondialdehyde, alpha feto protein and TNF alpha was biochemically estimated and tabulated. There was increase mean concentration of glutathione, malondialdehyde, alpha feto protein and TNF alpha in carcinoma group and PMD group when compared to control group (p<0.05). Glutathione, Malondialdehyde, TNF alpha and alpha Feto protein are found to increase gradually from potentially malignant disorder to malignant condition. These factors can be used as potential biomarkers to indicate the prognosis of the disease and can be used as diagnostic tool for screening and early detection.