scholarly journals Prevalence of Argulus indicus, histopathology and hematological properties of infected wild fish in Lake Towuti, Indonesia

2021 ◽  
Vol 22 (8) ◽  
Author(s):  
Amriana Amriana ◽  
Kesuma D. Sari ◽  
Sriwulan Sriwulan ◽  
Hilal Anshary
Keyword(s):  
Inflammatory Responses ◽  
Population Level ◽  
Infected Fish ◽  
Wild Fish ◽  
Infection Level ◽  
Anabas Testudineus ◽  
Mean Intensity ◽  
Climbing Perch ◽  
And Control ◽  
Lake Towuti

Abstract. Amriana, Sari DK, Sriwulan, Anshary H. 2021. Prevalence of Argulus indicus, histopathology and hematological properties of infected wild fish in Lake Towuti, Indonesia. Biodiversitas 22: 3578-3584. Parasites are disease agents that can threaten the health and survival of wild fish as individuals and at a population level. This study aimed to improve knowledge on the prevalence of Argulus indicus, infestation rates and the pathological effects of parasite infestation on hosts as well as hematological properties of infected fish. This study can inform future studies on the prevention and control of the cases of Argulus indicus infestation. Fish from Lake Towuti (20 45? 0? S, 1210 30? 0? E) were caught from February to May 2019 using traps and gill nets. The total of 373 specimens obtained comprised 102 climbing perch (Anabas testudineus), 74 three-spot cichlids (Cichlasoma trimaculatum), 84 Nile tilapia (Oreochromis niloticus), and 113 striped snakeheads (Channa striata). The prevalence, mean intensity and abundance of A. indicus were highest in C. striata with a prevalence of 81%, mean intensity of 5.17 parasites/fish and abundance of 4.06 parasites/fish. The lowest parasite infection level was seen in C. trimaculatum with a prevalence of 4.1%, mean intensity 1.66 and mean abundance of 0.06. Histological analysis showed inflammatory responses in the skin of snakeheads (hemorrhage, increased spread of melanomacrophage and leukocyte cells), melanomacrophage and the spread of leukocyte cells were observed in climbing perch. In contrast, melanomacrophage was often observed in tilapia and trimac cichlid. Blood imaging analysis showed significant differences (P <0.05) in the number of leukocytes and percentage of monocyte cells between uninfested fish and those infested with A. indicus.

scholarly journals Proximate composition, amino acids and fatty acids profiles of wild and cultured Climbing Perch, Anabas testudineus (Bloch, 1795)

2021 ◽  
Vol 48 (2) ◽  
pp. 365-378
Author(s):  
Mst Taslima Akter ◽  
Md Hasan Faruque ◽  
Md Rakibul Hasan ◽  
Mohammad Shamsur Rahman
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Amino Acid ◽  
Monsoon Season ◽  
Wild Fish ◽  
Anabas Testudineus ◽  
Climbing Perch ◽  
Post Monsoon ◽  
Cultured Fish

The availability and compositions of foods in the aquatic system have a direct influence on nutritional compositions of fish muscle. This research aimed to determine the nutritional compositions of wild and cultured climbing perch, Anabas testudineus, between pre-monsoon and post-monsoon. The proximate compositions, total amino acid, and fatty acid profiles were determined by AOAC, HPLC and GC, respectively. The results in dicated that proximate (e.g., moisture, ash, protein and lipid), amino acid and fatty acid profile varied significantly (p<0.05) between wild and cultured fishes depend on season. The highest amount of moisture (79.62±0.92%) was found in wild A. testudineus and lowest amount of ash (2.50±0.09%) was found in cultured A. testudineus during pre-monsoon. For both cultured and wild A. testudineus, amount of protein was higher in post-monsoon than in pre-monsoon period. The protein and amino acids contents as well as lipid and fatty acids of the fish species showed a significant (p<0.05) seasonal variations. Among the 14 recorded amino acidsthe major amino acids in the total crude protein were histidine, methionine, isoleucine, lysine, glutamic acid, and glycine. The ratio of Essential Amino Acid (EA)/Non-Essential Amino Acid (NEA) was highest (0.34) in cultured fish of post-monsoon season and lowest (0.25) in wild fish of pre-monsoon season. The major fatty acid so wild and cultured A. testudineus were oleic acid (18:1), palmitic acid (16:0), linoleic acid (18:2), stearic acid (18:0), palmitoleic acid (16:1), andmyristic acid (14:0).Some fatty acids [Lauric acid (C12:0), Pentad cyclic acid (C15:0), Arachidic acid (C20:0)]were not detected in cultured fish but present in wild fish. The n-3/n-6 ratio was highest (0.48) in wild fish of post-monsoon season and lowest (0.14) in cultured fish of pre-monsoon season. Therefore, a comprehensive study on wild and cultured climbing perch is required for further confirmation of the result of the present study. Bangladesh J. Zool. 48(2): 365-378, 2020

scholarly journals Experimental Infection of Indigenous Climbing Perch Anabas testudineus with Aeromonas hydrophila Bacteria

2013 ◽  
Vol 22 (1-2) ◽  
pp. 105-114 ◽  
Author(s):  
M Farhad Hossain ◽  
M Mamnur Rahman ◽  
MA Sayed
Keyword(s):  
Body Weight ◽  
Water Temperature ◽  
Aeromonas Hydrophila ◽  
Bacterial Load ◽  
Infected Fish ◽  
Pathogenicity Test ◽  
Anabas Testudineus ◽  
Climbing Perch ◽  
Average Body ◽  
Average Body Weight

The present study was conducted to know the pathogenicity and LD50 of Aeromonas hydrophila isolated from diseased climbing perch Anabas testudineus against apparently healthy homologous fish and the distribution of the bacteria in the organs of the experimentally infected fish. A total of 10 fish of average body weight of 18 g were used. For pathogenicity test, two different doses viz. 9.2 × 107 and 9.2 × 106 CFU/fish were injected intramuscularly. Pathogenicity of A. hydrophila was confirmed at water temperature of 28.53oC by the mortality of 40% to 100% of all tested fish within 4 to 9 days. The highest bacterial load was found to be 9.4 × 108 CFU/g in the intestine and the lowest bacterial load was found to be 2.8 × 103 CFU/g in the kidney of the tested fish. Four different serial concentrations, vide 9.2 × 107, 9.2 × 106, 9.2 × 105 and 9.2 × 104 CFU/fish of the bacteria were injected in each of four different groups of 10 fish. The calculated LD50 value at 27.3oC water temperature was 2 × 107 CFU/fish of 18 g of average body weight. In all the cases of intramuscular injection, external pathology was found. Reddish anal region and fin bases were observed. Injected A. hydrophila was re-isolated from liver, kidney and intestine of the challenged fish. It was understood that the isolate was a high virulent pathogen for A. testudineus.DOI: http://dx.doi.org/10.3329/pa.v22i1-2.16472 Progress. Agric. 22(1 & 2): 105-114, 2011

scholarly journals Experimental Horizontal Transmission of Enterospora nucleophila (Microsporea: Enterocytozoonidae) in Gilthead Sea Bream (Sparus aurata)

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 362
Author(s):  
Amparo Picard-Sánchez ◽  
M. Carla Piazzon ◽  
Itziar Estensoro ◽  
Raquel Del Pozo ◽  
Nahla Hossameldin Ahmed ◽  
...  
Keyword(s):  
Sparus Aurata ◽  
Horizontal Transmission ◽  
Infected Fish ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Target Tissue ◽  
Farmed Fish ◽  
Infection Level ◽  
Available Information

Enterospora nucleophila is a microsporidian enteroparasite that infects mainly the intestine of gilthead sea bream (Sparus aurata), leading to an emaciative syndrome. Thus far, the only available information about this infection comes from natural outbreaks in farmed fish. The aim of the present study was to determine whether E. nucleophila could be transmitted horizontally using naturally infected fish as donors, and to establish an experimental in vivo procedure to study this host–parasite model without depending on natural infections. Naïve fish were exposed to the infection by cohabitation, effluent, or intubated either orally or anally with intestinal scrapings of donor fish in four different trials. We succeeded in detecting parasite in naïve fish in all the challenges, but the infection level and the disease signs were always milder than in donor fish. The parasite was found in peripheral blood of naïve fish at 4 weeks post-challenge (wpc) in oral and effluent routes, and up to 12 wpc in the anal transmission trial. Molecular diagnosis detected E. nucleophila in other organs besides intestine, such as gills, liver, stomach or heart, although the intensity was not as high as in the target tissue. The infection tended to disappear through time in all the challenge routes assayed, except in the anal infection route.

scholarly journals Isolation and characterization of two virulent Aeromonads associated with haemorrhagic septicaemia and tail-rot disease in farmed climbing perch Anabas testudineus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Abhishek Mazumder ◽  
Hrishikesh Choudhury ◽  
Abhinit Dey ◽  
Dandadhar Sarma
Keyword(s):  
Natural Infection ◽  
Winter Season ◽  
Pcr Amplification ◽  
Body Parts ◽  
Anabas Testudineus ◽  
Climbing Perch ◽  
Isolation And Characterization ◽  
Biochemical Characterisation ◽  
Rot Disease

AbstractDiseased Anabas testudineus exhibiting signs of tail-rot and ulcerations on body were collected from a fish farm in Assam, India during the winter season (November 2018 to January 2019). Swabs from the infected body parts were streaked on sterilized nutrient agar. Two dominant bacterial colonies were obtained, which were then isolated and labelled as AM-31 and AM-05. Standard biochemical characterisation and 16S rRNA and rpoB gene sequencing identified AM-31 isolate as Aeromonas hydrophila and AM-05 as Aeromonas jandaei. Symptoms similar to that of natural infection were observed on re-infecting both bacteria to disease-free A. testudineus, which confirmed their virulence. LC50 was determined at 1.3 × 104 (A. hydrophila) and 2.5 × 104 (A. jandaei) CFU per fish in intraperitoneal injection. Further, PCR amplification of specific genes responsible for virulence (aerolysin and enterotoxin) confirmed pathogenicity of both bacteria. Histopathology of kidney and liver in the experimentally-infected fishes revealed haemorrhage, tubular degeneration and vacuolation. Antibiotic profiles were also assessed for both bacteria. To the best of our knowledge, the present work is a first report on the mortality of farmed climbing perch naturally-infected by A. hydrophila as well as A. jandaei, with no records of pathogenicity of the latter in this fish.

scholarly journals Parasitism of Cichlasoma paranaense Kullander, 1983 and Gymnotus carapo Linnaeus, 1814 by Clinostomum complanatum (Rudolphi, 1814) Metacercariae in the Taquari river

2005 ◽  
Vol 65 (3) ◽  
pp. 513-519 ◽  
Author(s):  
A. T. Silva-Souza ◽  
G. Ludwig
Keyword(s):  
Condition Factor ◽  
Infected Fish ◽  
Expected Value ◽  
Yellow Spot ◽  
Total Weight ◽  
Intensity Of Infection ◽  
Mean Intensity ◽  
Spot Disease ◽  
Relative Condition Factor ◽  
Clinostomum Complanatum

The infection, known as yellow spot disease, produced by metacercariae of Clinostomum spp. was studied in fishes of the Taquari river, located in Jataizinho, Paraná State, Brazil. A total of 1,582 specimens, belonging to 36 species, were collected between March 1999 and April 2001. Yellow spot disease was observed only in Gymnotus carapo Linnaeus, 1814 (Gymnotiformes, Gymnotidae) and Cichlasoma paranaense Kullander, 1983 (Perciformes, Cichlidae). This parasitism was generated by metacercariae of Clinostomum complanatum (Rudolphi, 1814) (Digenea, Clinostomidae). Among the 88 specimens of G. carapo examined, 7 (prevalence = 8%) had cysts of the parasite. Four of them were captured in July 1999 and three in October 1999. In the other months, no specimen found was infected. Using relative condition factor (Kn) analysis, it was determined that both infected and non-infected specimens had a total weight equal to the theoretically expected value for each total length (Kn = 1.0). Among 56 individuals of Cichlasoma paranaense, 6 (prevalence = 10.7%) had between 1 and 27 metacercariae of C. complanatum (mean intensity of infection = 9.3 ± 9.6). In March 1999 and April of both 2000 and 2001, the specimens examined were not infected. The infected fish had a total weight higher than the expected value (Kn > 1.0), while the non-infected fish had a weight equal to the expected value (Kn = 1.0).

scholarly journals Population-based virucidal phthalocyanine gargle/rinse protocol to reduce the risk of coronavirus disease-2019: a community trial

2021 ◽  
Author(s):  
Verônica Caroline Brito Reia ◽  
Roosevelt da Silva Bastos ◽  
Fabiano Vieira Vilhena ◽  
Heitor Marques Honório ◽  
Lucas Marques da Costa Alves ◽  
...  
Keyword(s):  
Relative Risk ◽  
Disease Risk ◽  
Phase 1 ◽  
Population Level ◽  
Population Based ◽  
Phase 2 ◽  
Control Community ◽  
And Control ◽  
Official Information ◽  
Community Trial

Abstract Purpose: In this community trial, the objective was to evaluate the incidence of coronavirus disease-2019 (COVID-19) cases in two similar communities in three distinct phases: phase 1 (before the intervention), 2 (during the intervention), and 3 (after the intervention).Methods: The test community received the oral antiseptic intervention (experimental), while the control community did not. The official information agency provided the number of confirmed COVID-19 cases. Data were analyzed according to the three phases per epidemiological week (epi) using the R Core Team (2021) program. The relative risk and 95% confidence intervals between the cumulative incidence values of the test and control communities were calculated for each period. In the test community, a total of 995 residents, over 10 years of age, received two bottles containing 600 ml of mouthwash with antiviral phthalocyanine derivative (APD). The participants were asked to gargle/rinse with of 5 mL of the mouthwash with ADP 3 to 5 times a day, for 1 min, until the end of the bottles. Results: In phases 1 and 3, disease risk between the two communities did not differ significantly (p> 0.05), while in phase 2, disease risk was 54% lower in the test community than in the control community. Conclusion: The use of the APD mouthwash protocol seems to reduce the COVID-19 incidence at the population level, and further studies are needed to confirm its protective effect in different contexts.

scholarly journals Pengaruh C/N Rasio Berbeda untuk Pembentukan Bioflok pada Media Pemeliharaan terhadap Pertumbuhan dan Kelangsungan Hidup Benih Ikan Betok (Anabas testudineus)

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
M. Indra Wahyu Pratama ◽  
Dade Jubaedah ◽  
Mohamad Amin
Keyword(s):  
Survival Rate ◽  
Culture Media ◽  
Farming System ◽  
Anabas Testudineus ◽  
Growth And Survival ◽  
Climbing Perch ◽  
Randomized Design ◽  
Absolute Growth ◽  
Biofloc Technology ◽  
Absolute Length

Pratama et al, 2018. The Effect of Different C/N Ratio in for Biofloc of Formation Culture Media to the Growth and Survival Rate of Juvenile Climbing Perch (Anabas testudineus). JLSO 7(1): Climbing perch fish resources still have a problem due to this fish is still obtained only through capture and also the growth of climbing perch fish is slow. The solutions to increase the growth of the climbing perch fish is by applying biofloc technology for climbing perch fish culture. The purpose of this research was to know the effect of different C/N ratio on the growth of climbing perch fish (Anabas testudineus) juvenille with biofloc farming system. This research used a completely randomized design consist of four treatments and three replications. The treatments were without molase (P0), molase with C/N ratio 15 (P1), C/N 20 (P2) and C/N ratio 25 (P3). The results showed that C / N ratio 20 was the best treatment with absolute length of growth of 1.33 cm, absolute growth of weight 1.92 g, survival rate of 99.17% and feed efficiency 138.06%.

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