Microdeformation of RBCs under oxidative stress measured by digital holographic microscopy and optical tweezers

2019 ◽  
Vol 58 (15) ◽  
pp. 4042
Author(s):  
Jiaqi Liu ◽  
Lianqing Zhu ◽  
Fan Zhang ◽  
Mingli Dong ◽  
Xinghua Qu
Keyword(s):  
Oxidative Stress ◽  
Optical Tweezers ◽  
Digital Holographic Microscopy ◽  
Holographic Microscopy

3D manipulation and visualization of in-vitro cells by optical tweezers and digital holographic microscopy

2014 ◽  
Author(s):  
F. Merola ◽  
L. Miccio ◽  
P. Memmolo ◽  
G. Di Caprio ◽  
G. Coppola ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Digital Holographic Microscopy ◽  
3D Manipulation ◽  
Holographic Microscopy

scholarly journals Biomechanics of Ex Vivo-Generated Red Blood Cells Investigated by Optical Tweezers and Digital Holographic Microscopy

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 552
Author(s):  
Claudia Bernecker ◽  
Maria Augusta R. B. F. Lima ◽  
Catalin D. Ciubotaru ◽  
Peter Schlenke ◽  
Isabel Dorn ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Optical Tweezers ◽  
Blood Cells ◽  
Ex Vivo ◽  
Single Cells ◽  
Membrane Lipid ◽  
Membrane Properties ◽  
Digital Holographic Microscopy ◽  
Holographic Microscopy

Ex vivo-generated red blood cells are a promising resource for future safe blood products, manufactured independently of voluntary blood donations. The physiological process of terminal maturation from spheroid reticulocytes to biconcave erythrocytes has not been accomplished yet. A better biomechanical characterization of cultured red blood cells (cRBCs) will be of utmost interest for manufacturer approval and therapeutic application. Here, we introduce a novel optical tweezer (OT) approach to measure the deformation and elasticity of single cells trapped away from the coverslip. To investigate membrane properties dependent on membrane lipid content, two culture conditions of cRBCs were investigated, cRBCPlasma with plasma and cRBCHPL supplemented with human platelet lysate. Biomechanical characterization of cells under optical forces proves the similar features of native RBCs and cRBCHPL, and different characteristics for cRBCPlasma. To confirm these results, we also applied a second technique, digital holographic microscopy (DHM), for cells laid on the surface. OT and DHM provided related results in terms of cell deformation and membrane fluctuations, allowing a reliable discrimination between cultured and native red blood cells. The two techniques are compared and discussed in terms of application and complementarity.

Combining digital holographic microscopy and optical tweezers: a new route in microfluidic

2012 ◽  
Author(s):  
L. Miccio ◽  
P. Memmolo ◽  
F. Merola ◽  
M. Paturzo ◽  
A. Finizio ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Digital Holographic Microscopy ◽  
Holographic Microscopy
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