Three-Dimensional Super-Resolution Single-Molecule Fluorescence Imaging Using a Double-Helix Point Spread Function

2009 ◽  
Author(s):  
Michael A. Thompson ◽  
Sri Rama. Pavani ◽  
Julie S. Biteen ◽  
Rafael Piestun ◽  
W. E. Moerner
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Point Spread Function ◽  
Double Helix ◽  
Three Dimensional ◽  
Super Resolution ◽  
Single Molecule Fluorescence ◽  
Point Spread ◽  
Spread Function ◽  
Resolution Single

scholarly journals Three-Dimensional Super-Resolution in Eukaryotic Cells Using the Double-Helix Point Spread Function

2017 ◽  
Vol 112 (7) ◽  
pp. 1444-1454 ◽  
Author(s):  
Alexander R. Carr ◽  
Aleks Ponjavic ◽  
Srinjan Basu ◽  
James McColl ◽  
Ana Mafalda Santos ◽  
...  
Keyword(s):  
Point Spread Function ◽  
Double Helix ◽  
Three Dimensional ◽  
Super Resolution ◽  
Eukaryotic Cells ◽  
Point Spread ◽  
Spread Function

scholarly journals Metasurface integrated with double-helix point spread function and metalens for three-dimensional imaging

Nanophotonics ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 451-458 ◽  
Author(s):  
Chunqi Jin ◽  
Jihua Zhang ◽  
Chunlei Guo
Keyword(s):  
Particle Tracking ◽  
Point Spread Function ◽  
Double Helix ◽  
Three Dimensional ◽  
Super Resolution ◽  
Single Particle Tracking ◽  
Point Sources ◽  
Phase Mask ◽  
Point Spread ◽  
Spread Function

AbstractMetasurfaces are two-dimensional arrangements of antennas that control the propagation of electromagnetic waves with a subwavelength thickness and resolution. Previously, metasurfaces have been mostly used to obtain the function of a single optical element. Here, we demonstrate a plasmonic metasurface that represents the combination of a phase mask generating a double-helix point spread function (DH-PSF) and a metalens for imaging. DH-PSF has been widely studied in three-dimensional (3D) super-resolution imaging, biomedical imaging, and particle tracking, but the current DH-PSFs are inefficient, bulky, and difficult to integrate. The multielement metasurface, which we label as DH-metalens, enables a DH-PSF with transfer efficiency up to 70.3% and an ultrahigh level of optical system integration, three orders of magnitude smaller than those realized by conventional phase elements. Moreover, the demonstrated DH-metalens can work in broadband visible wavelengths and in multiple incident polarization states. Finally, we demonstrate the application of the DH-metalens in 3D imaging of point sources. These results pave ways for realizing integrated DH-PSFs, which have applications in 3D super-resolution microscopy, single particle tracking/imaging, and machine vision.

scholarly journals Correlations of three-dimensional motion of chromosomal loci in yeast revealed by the double-helix point spread function microscope

2014 ◽  
Vol 25 (22) ◽  
pp. 3619-3629 ◽  
Author(s):  
Mikael P. Backlund ◽  
Ryan Joyner ◽  
Karsten Weis ◽  
W. E. Moerner
Keyword(s):  
Point Spread Function ◽  
Double Helix ◽  
Dynamical Behavior ◽  
Three Dimensional ◽  
Mean Square Displacement ◽  
Single Particle Tracking ◽  
3D Tracking ◽  
Point Spread ◽  
Spread Function ◽  
Diploid Cells

Single-particle tracking has been applied to study chromatin motion in live cells, revealing a wealth of dynamical behavior of the genomic material once believed to be relatively static throughout most of the cell cycle. Here we used the dual-color three-dimensional (3D) double-helix point spread function microscope to study the correlations of movement between two fluorescently labeled gene loci on either the same or different budding yeast chromosomes. We performed fast (10 Hz) 3D tracking of the two copies of the GAL locus in diploid cells in both activating and repressive conditions. As controls, we tracked pairs of loci along the same chromosome at various separations, as well as transcriptionally orthogonal genes on different chromosomes. We found that under repressive conditions, the GAL loci exhibited significantly higher velocity cross-correlations than they did under activating conditions. This relative increase has potentially important biological implications, as it might suggest coupling via shared silencing factors or association with decoupled machinery upon activation. We also found that on the time scale studied (∼0.1–30 s), the loci moved with significantly higher subdiffusive mean square displacement exponents than previously reported, which has implications for the application of polymer theory to chromatin motion in eukaryotes.

scholarly journals 3D Single-Molecule Super-Resolution Fluorescence Microscopy with the Corkscrew Point Spread Function

2016 ◽  
Vol 110 (3) ◽  
pp. 176a
Author(s):  
Maurice Lee ◽  
Matthew Lew ◽  
Alex von Diezmann ◽  
Lucien Weiss ◽  
Yoav Shechtman ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Point Spread Function ◽  
Super Resolution ◽  
Point Spread ◽  
Spread Function

The changing point-spread function: single-molecule-based super-resolution imaging

2014 ◽  
Vol 141 (6) ◽  
pp. 577-585 ◽  
Author(s):  
Mathew H. Horrocks ◽  
Matthieu Palayret ◽  
David Klenerman ◽  
Steven F. Lee
Keyword(s):  
Single Molecule ◽  
Point Spread Function ◽  
Super Resolution ◽  
Point Spread ◽  
Spread Function ◽  
Resolution Imaging
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