In-Flow Imaging by Multispectral Digital Holographic Microscopy with Partially Coherent Illumination

2015 ◽  
Author(s):  
Jérôme Dohet-Eraly ◽  
Catherine Yourassowsky ◽  
Frank Dubois
Keyword(s):  
Digital Holographic Microscopy ◽  
Flow Imaging ◽  
Partially Coherent ◽  
Holographic Microscopy ◽  
Coherent Illumination

scholarly journals Partially Coherent Illumination Based Point-Diffraction Digital Holographic Microscopy Study Dynamics of Live Cells

2021 ◽  
Vol 9 ◽  
Author(s):  
Kequn Zhuo ◽  
Yu Wang ◽  
Yang Wang ◽  
Kai Wen ◽  
Min Liu ◽  
...  
Keyword(s):  
Speckle Noise ◽  
Microscopy Study ◽  
Digital Holographic Microscopy ◽  
Live Cells ◽  
Dynamic Processes ◽  
Label Free ◽  
Fringe Contrast ◽  
Partially Coherent ◽  
Holographic Microscopy ◽  
Coherent Illumination

This paper presents a partially coherent point-diffraction digital holographic microscopy (PC-pDHM) prototype and demonstrates its application in label-free imaging of the dynamic processes of live cells. In PC-pDHM, the light scattered by a rotating diffuser is coupled into a multi-mode fiber, and the output light is used as the partially coherent illumination (PCI), which reduces the speckle noise significantly in PC-pDHM. A polarization-grating is used to remold the object and the reference waves, and the fringe contrast of the generated hologram can be adjusted by changing the polarization of the illumination wave. Using the PC-pDHM prototype, transparent samples and notably the dynamic processes of live cells were imaged with high contrast and in a label-free manner, discovering the pathological mechanisms of biology in the cellular and sub-cellular levels.

scholarly journals Spatial spectrum of coherence signal for a defocused object images in digital holographic microscopy with partially spatially coherent illumination

2018 ◽  
Vol 42 (3) ◽  
pp. 414-423
Author(s):  
D. M. Klychkova ◽  
V. P. Ryabukho
Keyword(s):  
Numerical Simulation ◽  
High Frequency ◽  
Laser Light ◽  
Spatial Spectrum ◽  
Theoretical Description ◽  
Digital Holographic Microscopy ◽  
Object Image ◽  
Holographic Microscopy ◽  
Coherent Illumination ◽  
Good Agreement

We study the effect of a decrease in the magnitude of the coherence signal in high-frequency spatial spectrum for a defocused object image in transmission digital holographic microscopy with quasimonochromatic partially spatially coherent illumination. A theoretical description and results of the numerical simulation of the effect for a point scattering object are presented. The effect is experimentally studied by illuminating layered quasi-point scatterers with partially spatially coherent laser light obtained using a moving scatterer. The comparison of the experimental and theorybased numerical results shows them to be in good agreement.

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