scholarly journals Genome-Wide High-Resolution Mapping of UV-Induced Mitotic Recombination Events in Saccharomyces cerevisiae

PLoS Genetics ◽  
2013 ◽  
Vol 9 (10) ◽  
pp. e1003894 ◽  
Author(s):  
Yi Yin ◽  
Thomas D. Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
High Resolution ◽  
Mitotic Recombination ◽  
High Resolution Mapping ◽  
Genome Wide ◽  
Resolution Mapping

scholarly journals Genome-Wide High-Resolution Mapping by Recurrent Intermating Using Arabidopsis thaliana as a Model

Genetics ◽  
1996 ◽  
Vol 142 (1) ◽  
pp. 247-258
Author(s):  
Sin-Chieh Liu ◽  
Stanley P Kowalski ◽  
Tien-Hung Lan ◽  
Kenneth A Feldmann ◽  
Andrew H Paterson
Keyword(s):  
High Resolution ◽  
Recombinant Inbred ◽  
Additional Information ◽  
High Resolution Mapping ◽  
Genome Wide ◽  
A Genome ◽  
High Resolution Analysis ◽  
Resolution Analysis ◽  
Resolution Mapping ◽  
Map Resolution

We demonstrate a method for developing populations suitable for genome-wide high-resolution genetic linkage mapping, by recurrent intermating among F2 individuals derived from crosses between homozygous parents. Comparison of intermated progenies to F2 and “recombinant inbred” (RI) populations from the same pedigree corroborate theoretical expectations that progenies intermated for four generations harbor about threefold more information for estimating recombination fraction between closely linked markers than either RI-selfed or F2 individuals (which are, in fact, equivalent in this regard). Although intermated populations are heterozygous, homozygous “intermated recombinant inbred” (IRI) populations can readily be generated, combining additional information afforded by intermating with the permanence of RI populations. Intermated populations permit fine-mapping of genetic markers throughout a genome, helping to bridge the gap between genetic map resolution and the DNA-carrying capacity of modern cloning vectors, thus facilitating merger of genetic and physical maps. Intermating can also facilitate high-resolution mapping of genes and QTLs, accelerating map-based cloning. Finally, intermated populations will facilitate investigation of other fundamental genetic questions requiring a genome-wide high-resolution analysis, such as comparative mapping of distantly related species, and the genetic basis of heterosis.

Sign in / Sign up

Export Citation Format

Share Document