scholarly journals Nasal Chemosensory-Stimulation Evoked Activity Patterns in the Rat Trigeminal Ganglion Visualized by In Vivo Voltage-Sensitive Dye Imaging

PLoS ONE ◽  
2011 ◽  
Vol 6 (10) ◽  
pp. e26158 ◽  
Author(s):  
Markus Rothermel ◽  
Benedict Shien Wei Ng ◽  
Agnieszka Grabska-Barwińska ◽  
Hanns Hatt ◽  
Dirk Jancke
Keyword(s):  
Trigeminal Ganglion ◽  
Activity Patterns ◽  
Voltage Sensitive Dye ◽  
Evoked Activity ◽  
Voltage Sensitive Dye Imaging

scholarly journals In vivo monitoring of chemically evoked activity patterns in the rat trigeminal ganglion

2013 ◽  
Vol 7 ◽  
Author(s):  
Matthias Lübbert ◽  
Jessica Kyereme ◽  
Markus Rothermel ◽  
Christian H. Wetzel ◽  
Klaus-Peter Hoffmann ◽  
...  
Keyword(s):  
Trigeminal Ganglion ◽  
Activity Patterns ◽  
In Vivo Monitoring ◽  
Evoked Activity

scholarly journals The anterior paired lateral neuron normalizes odour-evoked activity at the mushroom body calyx

2021 ◽  
Author(s):  
Luigi Prisco ◽  
Stephan Hubertus Deimel ◽  
Hanna Yeliseyeva ◽  
Andre Fiala ◽  
Gaia Tavosanis
Keyword(s):  
Mushroom Body ◽  
Activity Patterns ◽  
Sensory Information ◽  
Input Circuit ◽  
Projection Neurons ◽  
Neuronal Populations ◽  
Kenyon Cells ◽  
Presynaptic Boutons ◽  
Evoked Activity

To identify and memorize discrete but similar environmental inputs, the brain needs to distinguish between subtle differences of activity patterns in defined neuronal populations. The Kenyon cells of the Drosophila adult mushroom body (MB) respond sparsely to complex olfactory input, a property that is thought to support stimuli discrimination in the MB. To understand how this property emerges, we investigated the role of the inhibitory anterior paired lateral neuron (APL) in the input circuit of the MB, the calyx. Within the calyx, presynaptic boutons of projection neurons (PNs) form large synaptic microglomeruli (MGs) with dendrites of postsynaptic Kenyon cells (KCs). Combining EM data analysis and in vivo calcium imaging, we show that APL, via inhibitory and reciprocal synapses targeting both PN boutons and KC dendrites, normalizes odour-evoked representations in MGs of the calyx. APL response scales with the PN input strength and is regionalized around PN input distribution. Our data indicate that the formation of a sparse code by the Kenyon cells requires APL-driven normalization of their MG postsynaptic responses. This work provides experimental insights on how inhibition shapes sensory information representation in a higher brain centre, thereby supporting stimuli discrimination and allowing for efficient associative memory formation.

scholarly journals Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits

2020 ◽  
Author(s):  
Mária Ashaber ◽  
Yusuke Tomina ◽  
Pegah Kassraian ◽  
Eric A. Bushong ◽  
William B. Kristan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Motor Neuron ◽  
Activity Patterns ◽  
Medicinal Leech ◽  
Anatomical Connectivity ◽  
Voltage Sensitive Dye ◽  
Segmental Ganglion ◽  
The Relationship ◽  
Voltage Sensitive Dye Imaging

AbstractDorsal Excitor motor neuron DE-3 in the medicinal leech plays three very different dynamical roles in three different behaviors. Without rewiring its anatomical connectivity, how can a motor neuron dynamically switch roles to play appropriate roles in various behaviors? We previously used voltage-sensitive dye imaging to record from DE-3 and most other neurons in the leech segmental ganglion during (fictive) swimming, crawling, and local-bend escape (Tomina and Wagenaar, 2017). Here, we repeated that experiment, then re-imaged the same ganglion using serial blockface electron microscopy and traced all of DE-3’s processes. Further, we traced back the processes of all of DE-3’s presynaptic partners to their respective somata. This allowed us to analyze the relationship between circuit anatomy and the activity patterns it sustains. We found that input synapses important for all of the behaviors were widely distributed over DE-3’s branches, yet that functional clusters were different during (fictive) swimming vs. crawling.

scholarly journals The anterior paired lateral neuron normalizes odour-evoked activity in the Drosophila mushroom body calyx

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Luigi Prisco ◽  
Stephan Hubertus Deimel ◽  
Hanna Yeliseyeva ◽  
André Fiala ◽  
Gaia Tavosanis
Keyword(s):  
Mushroom Body ◽  
Activity Patterns ◽  
Sensory Information ◽  
Input Circuit ◽  
Projection Neurons ◽  
Neuronal Populations ◽  
Kenyon Cells ◽  
Presynaptic Boutons ◽  
Evoked Activity

To identify and memorize discrete but similar environmental inputs, the brain needs to distinguish between subtle differences of activity patterns in defined neuronal populations. The Kenyon cells of the Drosophila adult mushroom body (MB) respond sparsely to complex olfactory input, a property that is thought to support stimuli discrimination in the MB. To understand how this property emerges, we investigated the role of the inhibitory anterior paired lateral neuron (APL) in the input circuit of the MB, the calyx. Within the calyx, presynaptic boutons of projection neurons (PNs) form large synaptic microglomeruli (MGs) with dendrites of postsynaptic Kenyon cells (KCs). Combining EM data analysis and in vivo calcium imaging, we show that APL, via inhibitory and reciprocal synapses targeting both PN boutons and KC dendrites, normalizes odour-evoked representations in MGs of the calyx. APL response scales with the PN input strength and is regionalized around PN input distribution. Our data indicate that the formation of a sparse code by the Kenyon cells requires APL-driven normalization of their MG postsynaptic responses. This work provides experimental insights on how inhibition shapes sensory information representation in a higher brain centre, thereby supporting stimuli discrimination and allowing for efficient associative memory formation.

In vivo voltage-sensitive dye imaging of the insular cortex in nerve-injured rats

2016 ◽  
Vol 634 ◽  
pp. 146-152 ◽  
Author(s):  
Jeongsoo Han ◽  
Myeounghoon Cha ◽  
Minjee Kwon ◽  
Seong-Karp Hong ◽  
Sun Joon Bai ◽  
...  
Keyword(s):  
Insular Cortex ◽  
Voltage Sensitive Dye ◽  
Voltage Sensitive Dye Imaging

In Vivo Voltage-Sensitive Dye Imaging of Mammalian Cortex Using “Blue” Dyes

2015 ◽  
Vol 2015 (11) ◽  
pp. pdb.prot089359
Author(s):  
Bradley Baker ◽  
Xin Gao ◽  
Brian S. Wolff ◽  
Lei Jin ◽  
Lawrence B. Cohen ◽  
...  
Keyword(s):  
Voltage Sensitive Dye ◽  
Voltage Sensitive Dye Imaging

scholarly journals Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Mária Ashaber ◽  
Yusuke Tomina ◽  
Pegah Kassraian ◽  
Eric A Bushong ◽  
William B Kristan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Motor Neuron ◽  
Activity Patterns ◽  
Medicinal Leech ◽  
Anatomical Connectivity ◽  
Voltage Sensitive Dye ◽  
Segmental Ganglion ◽  
The Relationship ◽  
Voltage Sensitive Dye Imaging

Dorsal Excitor motor neuron DE-3 in the medicinal leech plays three very different dynamical roles in three different behaviors. Without rewiring its anatomical connectivity, how can a motor neuron dynamically switch roles to play appropriate roles in various behaviors? We previously used voltage-sensitive dye imaging to record from DE-3 and most other neurons in the leech segmental ganglion during (fictive) swimming, crawling, and local-bend escape (Tomina and Wagenaar, 2017). Here, we repeated that experiment, then re-imaged the same ganglion using serial blockface electron microscopy and traced DE-3’s processes. Further, we traced back the processes of DE-3’s presynaptic partners to their respective somata. This allowed us to analyze the relationship between circuit anatomy and the activity patterns it sustains. We found that input synapses important for all the behaviors were widely distributed over DE-3’s branches, yet that functional clusters were different during (fictive) swimming vs. crawling.

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