scholarly journals Rapid Identification and Classification of Listeria spp. and Serotype Assignment of Listeria monocytogenes Using Fourier Transform-Infrared Spectroscopy and Artificial Neural Network Analysis

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0143425 ◽  
Author(s):  
K. F. Romanolo ◽  
L. Gorski ◽  
S. Wang ◽  
C. R. Lauzon
2006 ◽  
Vol 73 (3) ◽  
pp. 1036-1040 ◽  
Author(s):  
Cecilia A. Rebuffo-Scheer ◽  
J�rgen Schmitt ◽  
Siegfried Scherer

ABSTRACT A classification system based on Fourier transform infrared (FTIR) spectroscopy combined with artificial neural network analysis was designed to differentiate 12 serovars of Listeria monocytogenes using a reference database of 106 well-defined strains. External validation was performed using a test set of another 166 L. monocytogenes strains. The O antigens (serogroup) of 164 strains (98.8%) could be identified correctly, and H antigens were correctly determined in 152 (91.6%) of the test strains. Importantly, 40 out of 41 potentially epidemic serovar 4b strains were unambiguously identified. FTIR analysis is superior to PCR-based systems for serovar differentiation and has potential for the rapid, simultaneous identification of both species and serovar of an unknown Listeria isolate by simply measuring a whole-cell infrared spectrum.


1993 ◽  
Vol 114 (1) ◽  
pp. 115-119 ◽  
Author(s):  
Jongsik Chun ◽  
Ekrem Atalan ◽  
Sam-Bong Kim ◽  
Hong-Joong Kim ◽  
Mohamed E. Hamid ◽  
...  

2013 ◽  
Vol 8 (1) ◽  
pp. 94 ◽  
Author(s):  
Andreas Lux ◽  
Ralf Müller ◽  
Mark Tulk ◽  
Carla Olivieri ◽  
Roberto Zarrabeita ◽  
...  

1997 ◽  
Vol 18 (9) ◽  
pp. 805-810 ◽  
Author(s):  
D. HAMILTON ◽  
D. OʼMAHONY ◽  
J. COFFEY ◽  
J. MURPHY ◽  
N. OʼHARE ◽  
...  

2004 ◽  
Vol 171 (4S) ◽  
pp. 502-503
Author(s):  
Mohamed A. Gomha ◽  
Khaled Z. Sheir ◽  
Saeed Showky ◽  
Khaled Madbouly ◽  
Emad Elsobky ◽  
...  

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