Early dynamic changes in circulating tumor cells and prognostic relevance following interventional radiological treatments in patients with hepatocellular carcinoma

The aim of this study was to investigate the dynamic changes of circulating tumor cells (CTCs) in patients with hepatocellular carcinoma (HCC) before and immediately after conducting a microwave ablation (MWA) and conventional transarterial chemoembolization (C-TACE). Additionally, the CTCs short-term dynamics were compared with the clinical course of the HCC-patients. Blood samples from 17 patients with HCC who underwent MWA (n = 10) or C-TACE (n = 7) were analyzed. Venous blood was taken before and immediately after the radiological interventions to isolate and quantify CTCs using flow cytometry. CTCs were identified as CD45- and positive for the markers ASGPR, CD146 and CD274 (PD-L1). Patients were followed of up to 2.2 years after the radiological intervention. CTCs were detected in 13 HCC patients (76%) prior to the radiological interventions. The rate of CTCs was significantly decreased after the intervention in patients treated with MWA (0.4 CTCs/mL of blood, p = 0.031). However, no significant differences were observed in patients who received C-TACE (0.3 CTCs/mL of blood, p = 0.300). Overall, no correlation was found between the CTCs rate before and after the radiological intervention and recurrence rate of HCC. This preliminary data could confirm the tumoricidal effects of MWA in patients with HCC by significantly decreasing CTCs rate. In our study, we were able to detect CTCs in HCC patients using 3 different tumor markers. This preliminary data shows significant lower CTCs detected in response to MWA. However, large-scale randomized clinical trials are needed to determine the future role and the prognostic relevance of CTCs following this treatment.

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Dynamic changes of circulating tumor cells before and after hepatectomy and its influence factors in patients with hepatocellular carcinoma

HPB ◽

10.1016/j.hpb.2019.10.1555 ◽

2019 ◽

Vol 21 ◽

pp. S197

Author(s):

Shuilin dong ◽

Xiaoping Chen ◽

Xin Luo ◽

Jingjing Yu ◽

Wei Xiao ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Tumor Cells ◽

Circulating Tumor Cells ◽

Influence Factors ◽

Dynamic Changes ◽

Before And After

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Characterization and potential clinical implications of different subtypes of circulating tumor cells in patients with hepatocellular carcinoma

Zeitschrift für Gastroenterologie ◽

10.1055/s-0032-1332085 ◽

2013 ◽

Vol 51 (01) ◽

Author(s):

I Nel ◽

HA Baba ◽

J Ertle ◽

F Weber ◽

B Sitek ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Tumor Cells ◽

Circulating Tumor Cells ◽

Clinical Implications

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Early Detection and Dynamic Changes of Circulating Tumor Cells in Transgenic NeuN Transgenic (NTTg) Mice with Spontaneous Breast Tumor Development

Cancers ◽

10.3390/cancers13133294 ◽

2021 ◽

Vol 13 (13) ◽

pp. 3294

Author(s):

Wen-Sy Tsai ◽

Tsung-Fu Hung ◽

Jia-Yang Chen ◽

Shu-Huan Huang ◽

Ying-Chih Chang

Keyword(s):

Tumor Cells ◽

Circulating Tumor Cells ◽

Breast Tumor ◽

Early Stage ◽

Tumor Development ◽

P Value ◽

Vascular Density ◽

Dynamic Changes ◽

Tumor Bearing ◽

Development Methods

Background: This study used NeuN transgenic (NTTg) mice with spontaneous breast tumor development to evaluate the dynamic changes of circulating tumor cells (CTCs) prior to and during tumor development. Methods: In this longitudinal, clinically uninterrupted study, we collected 75 μL of peripheral blood at the age of 8, 12, 16, and 20 weeks in the first group of five mice, and at the age of 32 weeks, the time of tumor palpability, and one week after tumor palpability in the second group of four mice. Diluted blood samples were run through a modified mouse-CMx chip to isolate the CTCs. Results: The CTC counts of the first group of mice were low (1 ± 1.6) initially. The average CTC counts were 16 ± 9.5, 29.0 ± 18.2, and 70.0 ± 30.3 cells per 75 μL blood at the age of 32 weeks, the time of tumor palpability, and one week after tumor palpability, respectively. There was a significant positive correlation between an increase in CTC levels and tumor vascular density (p-value < 0.01). This correlation was stronger than that between CTC levels and tumor size (p-value = 0.076). The captured CTCs were implanted into a non-tumor-bearing NTTg mouse for xenografting, confirming their viability and tumorigenesis. Conclusion: Serial CTCs during an early stage of tumor progression were quantified and found to be positively correlated with the later tumor vascular density and size. Furthermore, the successful generation of CTC-derived xenografts indicates the tumorigenicity of this early onset CTC population.

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Isolation of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Using a Novel Cell Separation Strategy

Clinical Cancer Research ◽

10.1158/1078-0432.ccr-10-0498 ◽

2011 ◽

Vol 17 (11) ◽

pp. 3783-3793 ◽

Cited By ~ 86

Author(s):

Wen Xu ◽

Lu Cao ◽

Lei Chen ◽

Jing Li ◽

Xiao-Feng Zhang ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Tumor Cells ◽

Circulating Tumor Cells ◽

Cell Separation ◽

Separation Strategy

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Significance of Circulating Tumor Cells Detected by the CellSearch System in Patients with Metastatic Breast Colorectal and Prostate Cancer

Journal of Oncology ◽

10.1155/2010/617421 ◽

2010 ◽

Vol 2010 ◽

pp. 1-8 ◽

Cited By ~ 379

Author(s):

M. Craig Miller ◽

Gerald V. Doyle ◽

Leon W. M. M. Terstappen

Keyword(s):

Prostate Cancer ◽

Tumor Cells ◽

Circulating Tumor Cells ◽

Treatment Options ◽

Venous Blood ◽

Metastatic Breast ◽

Cellsearch System ◽

Multicenter Studies ◽

Real Time Analysis ◽

Number Of Treatment

The increasing number of treatment options for patients with metastatic carcinomas has created a concomitant need for new methods to monitor their use. Ideally, these modalities would be noninvasive, be independent of treatment, and provide quantitative real-time analysis of tumor activity in a variety of carcinomas. Assessment of circulating tumor cells (CTCs) shed into the blood during metastasis may satisfy this need. We developed the CellSearch System to enumerate CTC from 7.5 mL of venous blood. In this review we compare the outcomes from three prospective multicenter studies investigating the use of CTC to monitor patients undergoing treatment for metastatic breast (MBC), colorectal (MCRC), or prostate cancer (MPC) and review the CTC definition used in these studies. Evaluation of CTC at anytime during the course of disease allows assessment of patient prognosis and is predictive of overall survival.

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