scholarly journals Circulating miR-147b as a diagnostic marker for patients with bacterial sepsis and septic shock

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0261228
Author(s):  
Ngo Tat Trung ◽  
Tran Thi Lien ◽  
Vu Viet Sang ◽  
Nghiem Xuan Hoan ◽  
Nguyen Dang Manh ◽  
...  
Keyword(s):  
Septic Shock ◽  
Diagnostic Marker ◽  
Antimicrobial Treatment ◽  
Patient Stratification ◽  
Circulating Biomarkers ◽  
Bacterial Sepsis ◽  
Diagnostic Potential ◽  
Sepsis And Septic Shock ◽  
Plasma Mirnas ◽  
Subsequent Patient

Background Early diagnosis, precise antimicrobial treatment and subsequent patient stratification can improve sepsis outcomes. Circulating biomarkers such as plasma microRNAs (miRNAs) have proven to be surrogates for diagnosis, severity and case management of infections. The expression of four selected miRNAs (miR-146-3p, miR-147b, miR-155 and miR-223) was validated for their prognostic and diagnostic potential in a clinically defined cohort of patients with sepsis and septic shock. Methods The expression of plasma miRNAs was quantified by quantitative PCR (qPCR) in patients with bacterial sepsis (n = 78), in patients with septic shock (n = 52) and in patients with dengue haemorrhagic fever (DHF; n = 69) and in healthy controls (n = 82). Results The expression of studied miRNA was significantly increased in patients with bacterial sepsis and septic shock. The plasma miR-147b was able to differentiate bacterial sepsis from non-sepsis and septic shock (AUC = 0.77 and 0.8, respectively, p≤ 0.05), while the combination of plasma miR-147b and procalcitonin (PCT) predicted septic shock (AUC = 0.86, p≤ 0.05). Conclusions The plasma miR-147b may be an useful biomarker independently or in combination with PCT to support clinical diagnosis of sepsis and equally prognosis of patients with septic shock.

scholarly journals Plasma interleukin-6 levels correlate with survival in patients with bacterial sepsis and septic shock

2021 ◽  
Vol 11 (4) ◽  
pp. 224-230
Author(s):  
Monica Chavez Vivas ◽  
Hector Fabio Villamarin Guerrero ◽  
Antonio Jose Tascon ◽  
Augusto Valderrama-Aguirre
Keyword(s):  
Septic Shock ◽  
Cox Regression ◽  
Serum Levels ◽  
Significant Decline ◽  
Bacterial Sepsis ◽  
Inflammatory Biomarker ◽  
Statistical Program ◽  
Sepsis And Septic Shock ◽  
Time Of Admission ◽  
Admission Variables

AbstractIn this study, IL-6 levels were assessed as inflammatory biomarker of bacterial sepsis in patients hospitalized at the ICU of the hospital of Colombia.Materials and methodsProspective study on 62 patients diagnosed with sepsis and septic shock. An ELISA assay was used to test serum levels of IL-6 at admission and 48 h after admission. Variables were analyzed by χ2 test (alfa <0.05). Multivariable Cox regression was used to determine the survival with the statistical program SPSS v23.00.ResultsPatient's median age was 53 years old and 59.7% were male. Lung was the most common primary site of infection (43.5%), and hypertension comorbidity with higher prevalence (40%). Infection by Gram negative bacteria were significantly more frequent among patients than Gram positive (P = 0.037). Overall, survival analysis showed that 10 (16.1%) patients died with a survival median of 7.00 +4.874 (2–3) days. In patients with sepsis we detected a significant decline in the average of IL-6 serum levels after 48 h of admission [7.50 (SD: 7.00–68.00) pg/mL vs. 68.00 [SD: 7.00–300.00] pg/mL (P = 0.000). Only 25% of patients with septic shock who presented high levels of IL-6 at the time of admission and at 48 h had a survival up to 15 days (P = 0.005).ConclusionWe found significant differences between the plasma levels of IL-6 during the first 48 h after admission to the ICU among patients with sepsis and septic shock. Patients with sepsis had a significant decline in IL-6 levels, whereas in patients who developed septic shock, levels of this cytokine remained high and have a lower survival compared to those who maintained low levels of IL-6.

scholarly journals The Role of Serum Procalcitonin in Predicting Bacterial Sepsis in Patients With Hypothyroidism

2019 ◽  
Vol 104 (12) ◽  
pp. 5915-5922
Author(s):  
Aditya S Shirali ◽  
James X Wu ◽  
Catherine Y Zhu ◽  
Alfonso Ocampo ◽  
Chi-Hong Tseng ◽  
...  
Keyword(s):  
Septic Shock ◽  
Test Performance ◽  
Serum Levels ◽  
Primary Hypothyroidism ◽  
Bacterial Sepsis ◽  
Thyroid C Cells ◽  
Failure Assessment ◽  
History Of ◽  
Sepsis And Septic Shock ◽  
Design And Methods

Abstract Context Serum levels of procalcitonin (PCT), a protein produced by the thyroid C cells under physiologic conditions, are high during sepsis. Objective To assess the test performance of serum PCT in predicting bacterial sepsis and septic shock in patients with hypothyroidism compared with those who have euthyroidism. Design and Methods This retrospective study evaluated patients with no history of thyroid dysfunction (euthyroid), primary hypothyroidism [medical hypothyroidism (MH)], and postsurgical hypothyroidism from total thyroidectomy (TT) identified from a prospectively maintained database who had PCT testing from 2005 to 2018. Quick Sequential Organ Failure Assessment score ≥ 2 or positive bacterial cultures identified bacterial sepsis, and a mean arterial pressure less than 65 mm Hg or a vasopressor requirement defined septic shock. Sensitivity and specificity of PCT for evaluation of bacterial sepsis and septic shock were measured. Results We identified 217 euthyroid patients, 197 patients with MH, and 84 patients with TT. Bacterial sepsis was found in 98 (45.2%), 92 (46.7%), and 36 (42.9%) of these patients, respectively (P > 0.05). Septic shock was identified in 13 (6.0%), 13 (6.6%), and 5 (6.0%) patients (P > 0.05), respectively. With use of a PCT cutoff of 0.5 µg/L for bacterial sepsis, the sensitivity was 59%, 61%, and 53% (P > 0.05) and specificity was 81%, 77%, and 81% (P > 0.05) for the diagnosis of bacterial sepsis in euthyroid, MH, and TT patients, respectively. With use of a PCT cutoff of 2.0 µg/L for septic shock, the sensitivity was 46%, 62%, and 63% (P > 0.05) and specificity was 86%, 82%, and 91% (P > 0.05) for the diagnosis of septic shock in these patients, respectively. Conclusions Despite the thyroidal origin of PCT, hypothyroidism did not affect the diagnostic performance of serum PCT levels in predicting bacterial sepsis or septic shock.

scholarly journals Interleukin-6 kinetics can be useful for early treatment monitoring of severe bacterial sepsis and septic shock

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
Thomas Klag ◽  
Giulio Cantara ◽  
Udo Sechtem ◽  
Anastasios Athanasiadis
Keyword(s):  
Septic Shock ◽  
Interleukin 6 ◽  
Early Treatment ◽  
Treatment Monitoring ◽  
Bacterial Sepsis ◽  
Case Histories ◽  
Sepsis And Septic Shock ◽  
Kinetics Of

Early appropriate anti-microbial therapy is necessary to improve outcomes of septic patients. We describe 20 case histories of patients with severe bacterial sepsis regarding kinetics of several biomarkers. We found that interleukin-6 is able to predict survival and might be able to evaluate appropriateness of anti-microbial therapy.

Thyroid Hormone Levels and their Relationship to Survival in Children with Bacterial Sepsis and Septic Shock

2004 ◽  
Vol 17 (10) ◽  
Author(s):  
D. Yıldızdaş ◽  
N. Önenli-Mungan ◽  
Η. Yapıcıoğlu ◽  
A.K. Topaloglu ◽  
Y. Sertdemir ◽  
...  
Keyword(s):  
Septic Shock ◽  
Thyroid Hormone ◽  
Bacterial Sepsis ◽  
Hormone Levels ◽  
Thyroid Hormone Levels ◽  
Sepsis And Septic Shock

scholarly journals Receptors, Mediators, and Mechanisms Involved in Bacterial Sepsis and Septic Shock

2003 ◽  
Vol 16 (3) ◽  
pp. 379-414 ◽  
Author(s):  
Edwin S. Van Amersfoort ◽  
Theo J. C. Van Berkel ◽  
Johan Kuiper
Keyword(s):  
Septic Shock ◽  
Mononuclear Cells ◽  
Lipoteichoic Acid ◽  
The Body ◽  
Plasma Lipoproteins ◽  
Bacterial Sepsis ◽  
Proinflammatory Mediators ◽  
Sepsis And Septic Shock ◽  
Bacterial Constituents

SUMMARY Bacterial sepsis and septic shock result from the overproduction of inflammatory mediators as a consequence of the interaction of the immune system with bacteria and bacterial wall constituents in the body. Bacterial cell wall constituents such as lipopolysaccharide, peptidoglycans, and lipoteichoic acid are particularly responsible for the deleterious effects of bacteria. These constituents interact in the body with a large number of proteins and receptors, and this interaction determines the eventual inflammatory effect of the compounds. Within the circulation bacterial constituents interact with proteins such as plasma lipoproteins and lipopolysaccharide binding protein. The interaction of the bacterial constituents with receptors on the surface of mononuclear cells is mainly responsible for the induction of proinflammatory mediators by the bacterial constituents. The role of individual receptors such as the toll-like receptors and CD14 in the induction of proinflammatory cytokines and adhesion molecules is discussed in detail. In addition, the roles of a number of other receptors that bind bacterial compounds such as scavenger receptors and their modulating role in inflammation are described. Finally, the therapies for the treatment of bacterial sepsis and septic shock are discussed in relation to the action of the aforementioned receptors and proteins.

Diagnostic Potential of Endotoxin Scattering Photometry for Sepsis and Septic Shock

Shock ◽  
2013 ◽  
Vol 40 (6) ◽  
pp. 504-511 ◽  
Author(s):  
Tomoharu Shimizu ◽  
Toru Obata ◽  
Hiromichi Sonoda ◽  
Hiroya Akabori ◽  
Tohru Miyake ◽  
...  
Keyword(s):  
Septic Shock ◽  
Diagnostic Potential ◽  
Sepsis And Septic Shock

scholarly journals What Do We Know about Early Management of Sepsis and Septic Shock in Polish Hospitals? A Questionnaire Study

Healthcare ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 140
Author(s):  
Łukasz J. Krzych ◽  
Agnieszka Wiórek ◽  
Paweł Zatorski ◽  
Karol Gruca ◽  
Karina Stefańska-Wronka ◽  
...  
Keyword(s):  
Septic Shock ◽  
Antimicrobial Treatment ◽  
Questionnaire Study ◽  
Web Based ◽  
Early Management ◽  
Sepsis Diagnosis ◽  
Fluid Load ◽  
Medical Emergencies ◽  
Knowledge And Attitude ◽  
Sepsis And Septic Shock

Background: Sepsis and septic shock are medical emergencies with a high risk of poor prognosis. We investigate the correspondence between Surviving Sepsis Campaign (SSC) guidelines and clinical practice in Poland, with special attention given to differences between ICU and non-ICU environments as well as regional variations within the country. Methods: A web-based questionnaire study was performed on a random sample of 60 hospitals from the three most populated regions in Poland—Masovia, Silesia, and Greater Poland. A 19-item questionnaire was built based on the most recent edition of SSC guidelines. Results: Sepsis diagnosis was primarily based on clinical evaluation (ICUs: 94%, non-ICUs: 62%; p = 0.02). There were significant differences between ICUs and non-ICUs regarding taking blood cultures for pathogen identification (2-times more frequent in ICUs) and having hospital-based operating procedures to adjust antimicrobial treatment to a clinical scenario (a difference of 17%). Modification of empiric antimicrobial treatment was required post-ICU admission in 70% of cases. ICUs differed from non-ICUs with regard to the methods of fluid responsiveness assessment and the types of catecholamines and fluids used to treat septic shock. The mean fluid load applied before the implementation of catecholamines was 25.8 ± 10.6 mL/kg. Norepinephrine was the first-line agent used to treat shock, and balanced crystalloids were preferred in both ICUs and non-ICUs. Conclusion: Compliance with SCC guidelines in Polish hospitals is insufficient, especially outside ICUs. There is a need for education among healthcare professionals to reach at least an acceptable level of knowledge and attitude in this field.

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