SUMMARYAn outbreak of cryptosporidiosis associated with exposure to outdoor swimming-pool water affected an estimated 800–1000 individuals. PCR products were obtained from faecal specimens from 30 individuals who tested positive forCryptosporidiumoocysts. RFLP and sequencing analyses showed that all individuals were infected withCryptosporidium parvum. Among the infected individuals, five had just swum in an adjacent indoor pool during the same period, and had no identified contact with individuals linked to the outdoor pool. With the use of subgenotyping based on analysis of three mini- and microsatellite loci, MS1, TP14, and GP15, we could identify two sources of exposure. One subtype was associated with the outdoor pool and another with the indoor pool. These data demonstrate that the use of mini- and microsatellite loci as markers for molecular fingerprinting ofC. parvumisolates are valuable in the epidemiological investigation of outbreaks.
Evaluation of Chemical Characteristics of Swimming Pool Water and Poly(dimethyldiallylammonium chloride) Coagulation with Inactive Cryptosporidium parvum Oocysts in Pool Water