scholarly journals α-Glucosidase Inhibitory Activity of Phenolic Rich Extracts Obtained from the Seeds of Melastoma saigonense (Kuntze) Merr.

2019 ◽  
Vol 31 (12) ◽  
pp. 2964-2968 ◽  
Author(s):  
Nutthamon Prajudtasri ◽  
Mongkol Nontakitticharoen ◽  
Sujint Anguravirutt
Keyword(s):  
Ethyl Acetate ◽  
Inhibitory Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Inhibitory Effect ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Antidiabetic Agent ◽  
Seed Extracts

The aim of this study was to perform a phytochemical analysis of Melastoma saigonense seed extracts and to determine their α-glucosidase inhibitory activity. The extracts from seeds of M. saigonense indicated that the total phenolic content was in the range between 233.46 and 967.22 mg GAE/g DE, whereas the flavonoids content was in the range between 359.96 and 850.84 mg QE/g DE. The present study of antidiabetic inhibitory activity by in vitro α-glucosidase revealed that the crude extracts using ethyl acetate (EA), butanol (BU) and final aqueous residue extracts (AQ) exhibited a strong α-glucosidase inhibitory effect (IC50 4.42-11.95 μg/mL). The ethyl acetate and butanol extracts of seeds of Melastoma saigonense (Kuntze) Merr. were further fractionated by silica gel column chromatography into four fractions (EAF1−EAF4) and five fractions (BUF1−BUF5), respectively and their bioactivities were investigated. The nine fractions exhibited significant α-glucosidase inhibitory activity (p < 0.05) with an IC50 between 3.42-34.77 μg/mL which is less than the IC50 for standard acarbose (IC50 = 507.26 μg/mL). Among all the fractions, BUF1 and EAF1 exhibited high inhibitory activity against α-glucosidase with BUF1 showing the highest inhibitory activity (IC50 = 3.42 μg/mL). The dominant phenolic acids were sinapic, gallic, ferrulic, syringic, gallic and caffeic acids and the prominent flavonoids were myricetin and quercetin. These findings suggest that the seeds of M. saigonense have potential as a source of antidiabetic agent (s).

scholarly journals Phytochemical Analysis and Antimicrobial Activity of Iris kashmiriana and Iris ensata Extracts against Selected Microorganisms

2018 ◽  
Vol 8 (6) ◽  
pp. 28-34
Author(s):  
Javeed Iqbal Wagay ◽  
Kirti Jain
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Iris Ensata ◽  
Total Flavonoids Content ◽  
Phytochemical Constituent

Infectious diseases are the second leading cause for worldwide death. Treatment of infections continues to be difficult in modern time because of the severe side effects of some drugs and the growing resistance to antibacterial agents. Over the past few decades the use of antibiotics is under threat as many commonly used antibiotics have become less effective against certain illnesses due to emergence of multi drug-resistant bacteria. In the present study the effects of 3 types of solvents, chloroform, ethyl acetate and methanol were investigated to determine the presence of various phytochemical constituent, total phenolic content, total flavonoids content and in vitro antimicrobial activity from rhizomes of Iris kashmiriana (Kashmir Iris) and Iris ensata (Japanese Iris), belong to family Iridaceae. The reason for selecting in vitro method was to minimize the usage of experimental animals. The antimicrobial activity of chloroform, ethyl acetate and methanol extract of rhizomes of Iris kashmiriana and Iris ensata were evaluated on bacterial strains of Bacillus cereus, Pseudomonas auregenosa, Proteus vulgaris and Eschirichia coli and fungal strains of Candida albicans and Aspergillus niger by agar well diffusion method. The preliminary phytochemical studies and quantitative analysis of alkaloids, phenol and flavonoids were performed by well reported method. These extracts were further subjected to TLC (Thin layer chromatography analysis).  The chemical contents of the Iris kashmiriana and Iris ensata were presented as total phenolic content and total flavonoids content. Phytochemical screening of the extract showed the presence of some common compounds like phenols, terpenoids, flavonoids, carbohydrate etc. The antimicrobial potential of the plant extract was evaluated against different bacterial species which shows significant inhibitory action against all the tested bacterial and fungal strain. Methanolic extract was found to be more active than chloroform and ethyl acetate extracts. It reveals that the methanol soluble components of the plant are highly active against the above mentioned microorganism. Keywords: Iris kashmiriana, Iris ensata, Phytochemical constituent, Total phenolic content, Total flavonoids content, In vitro antimicrobial activity

scholarly journals ESTIMATION OF TOTAL PHENOL AND ANTIOXIDANT ACTIVITY OF ZANTHOXYLUM ARMATUM OF NEPALESE ORIGIN

2020 ◽  
pp. 37-40
Author(s):  
DURGA DHAKAL ◽  
KHAGA RAJ SHARMA
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Hexane Extract ◽  
Total Phenolic ◽  
Zanthoxylum Armatum ◽  
In Vitro Antioxidant Activity

Objective: The aim of this study was to analyze the phytoconstituents, estimation of total phenolic content and in vitro antioxidant activity of Zanthoxylum armatum from Myagdi district of Nepal. Methods: The seeds extract of Zanthoxylum armatum was prepared by cold percolation in hexane, ethyl acetate and methanol with continuous agitation. Phytochemical analysis for each extracts was performed by color differentiation method adopting the standard protocol. Antioxidant potential of the extracts was performed by DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay by taking ascorbic acid as standard. Total phenolic content was estimated by Folin–Ciocalteu reagent method. Results: The analysis of secondary metabolites showed the presence of quinones and terpenoidsin almost all extracts while alkaloids, polyphenols, tannins and saponins were abundant in polar extracts. The results of antioxidant activity showed the methanol extract of Zanthoxylum armatum IC50 87.47µg/ml was found to be more antioxidant as compared to the standard ascorbic acid IC50 66.40 µg/ml. Ethyl acetate extract showed moderate antioxidant activity with IC50 142.04 µg/ml, whereas hexane extract showed the poor antioxidant activity with IC50 384.03 µg/ml. The result of total phenolic content showed, ethyl acetate fraction has the highest 26.28±9.35 mg GAE/g as compared to methanol extract 23.36±14.80 mg GAE/g and hexane extract showed the poor phenolic content 20.05±8.0 mg GAE/g. Conclusion: The seeds extracts of Zanthoxlum armatum were found the rich source of secondary metabolites. The methanol extract was found potent antioxidant as compared to ethyl acetate and hexane extract. It is concluded that further activity guided isolation approaches will be needed on methanol extract to identify the active compound responsible for in vivo and in vitro antioxidant activity.

scholarly journals Analisis Fitokimia dan Uji Toksisitas Daun Leleng Merah (Graptophyllum pictum (L.) Griffith) dengan Metode Brine Shrimp Lethality Test

2020 ◽  
Vol 20 (2) ◽  
pp. 122
Author(s):  
Defrikson Ba'u ◽  
Dewa G Katja ◽  
Vanda S Kamu ◽  
Paulina V.Y Yamlean ◽  
Max R.J Runtuwene
Keyword(s):  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Brine Shrimp ◽  
Phenolic Content ◽  
Condensed Tannin ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Brine Shrimp Lethality ◽  
Phytochemical Content ◽  
Water Solvent

Penelitian ini bertujuan untuk menentukan total phenolic content (TPC), total flavonoid content (TFC), condensed tannin content (CTC) dan nilai toksisitas dari daun leleng merah. Daun leleng merah diekstraksi dengan metode maserasi dan dipartisi menggunakan pelarut n-heksana, etil asetat, dan air. Penentuan nilai TPC menggunakan metode Folin-Ciocalteu. Penentuan nilai TFC menggunakan metode AlCl3. Penentuan nilai CTC menggunakan metode vanillin-HCl. Penentuan nilai toksisitas menggunakan metode BSLT. Nilai TPC yang diperoleh pada fraksi n-heksana (FH), fraksi air (FA), dan fraksi etil asetat (FEA) daun Leleng Merah berturut-turut, adalah: 20,48; 31,7; dan 37,57 (mg/g). Nilai TFC diperoleh pada FH, FA, dan FEA berturut-turut, adalah: 0,64; 0,99; dan 1,27 (mg/g). Nilai CTC diperoleh pada FH, FA, dan FEA berturut-turut, adalah: 0,56; 2,17; dan 6,52 (mg/g). Nilai toksisitas diperoleh pada FEA, FA, FH berturut-turut, adalah: 21,93; 100,7; dan 117,22 (mg/L). FEA memiliki nilai toksisitas dan kandungan fitokimia tertinggi. Daun leleng merah bersifat toksik.Kata Kunci : Brine shrimp lethality test, leleng merah, fitokimia, Phytochemical Analysis and Toxicity Test of Leleng Merah Leaves (Graptophyllum pictum (L.) Griffith) Using the Brine Shrimp Lethality Test ABSTRACTThis study aims to determine the total phenolic content (TPC), flavonoids (TFC), condensed tannins (CTC) and toxicity value of Leleng Merah leaves. Leleng merah leaves were extracted by maceration method and partitioned using n-hexane, ethyl acetate, and water solvent. TPC values were determined using the Folin-Ciocalteu method. TFC values were determined using the AlCl3 method. CTC values were determined using the vanillin-HCl method. Toxicity values were determined using the BSLT method. TPC values obtained in the n-hexane (FH), water (FA), and ethyl acetate (FEA) fraction of the Leleng Merah leaves, were: 20.48; 31.7; 37.57 (mg / g) respectively. TFC values obtained in FH, FA, and FEA, were : 0.64; 0.99; 1.27 (mg / g) respectively. CTC values obtained at FH, FA, FEA, were: 0.56; 2.17;  6.52 (mg/g) respectively. Toxicity values obtained at FEA, FA, FH, were: 21.93; 100.7; 117.22 (mg/L) respectively. FEA has the highest toxicity and phytochemical content. Leleng Merah Leaf is toxic.Keywords : Brine Shrimp Lethality Test, leleng merah, phytochemistry

Effect of 1-MCP on Bioavailability of Lingwu Long Jujube In Vitro Digestion

2012 ◽  
Vol 554-556 ◽  
pp. 1357-1360
Author(s):  
Zhong Li Jiang ◽  
Ai Li Wang ◽  
Xi Hong Li ◽  
Min Peng Zhu ◽  
Jun Wei Wang
Keyword(s):  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Control Samples

The present study investigated the effect of 1-MCP on bioavailability of Lingwu long jujube stored at 0 °C for 60 days. At the end of the storage, compared with the control samples, Lingwu long jujube treated with 1-MCP exhibited higher Vc bioaccessibility, total phenolic content and free radical scavenging capacity, which demonstrated that 1-MCP treatment could provide a better effect on maintaining the bioavailability of Lingwu long jujube.

scholarly journals ANGIOTENSIN CONVERTING ENZYME INHIBITORY ACTIVITY OF MELINJO (GNETUM GNEMON L.) SEED EXTRACTS AND MOLECULAR DOCKING OF ITS STILBENE CONSTITUENTS

2017 ◽  
Vol 10 (3) ◽  
pp. 243
Author(s):  
Abdul Mun'im ◽  
Muhammad Ashar Munadhil ◽  
Nuraini Puspitasari ◽  
Azminah . ◽  
Arry Yanuar
Keyword(s):  
Molecular Docking ◽  
Angiotensin Converting Enzyme ◽  
Ethyl Acetate ◽  
Inhibitory Activity ◽  
Phenolic Content ◽  
Ethyl Acetate Extract ◽  
Total Phenolic ◽  
Converting Enzyme ◽  
Gnetum Gnemon ◽  
Ace Inhibitory

ABSTRACTObjectives: To evaluate the angiotensin converting enzyme (ACE) inhibitory activity of melinjo (Gnetum gnemon) seed extract and to study moleculardocking of stilbene contained in melinjo seeds.Methods: Melinjo seed powders were extracted with n-hexane, dichloromethane, ethyl acetate, methanol, and water successively. The extracts wereevaluated ACE inhibitory activities using ACE kit-Wist and the phenolic content using Folin–Ciocalteu method. The extract demonstrated the highestACE inhibitory activity was subjected to liquid chromatography-mass spectrometry (LC-MS) to know its stilbene constituent. The stilbene constituentsin melinjo seed were performed molecular docking using AutoDock Vina, and ligand-receptor Interactions were processed using Ligand Scout.Results: The ethyl acetate extract demonstrated the highest ACE inhibition activity with inhibitory concentration 50% value of 9.77 × 10−8 μg/mLand the highest total phenolic content (575.9 mg gallic acid equivalent/g). Ultra-performance LC-MS analysis of ethyl acetate extract has detected theexistency of resveratrol, gnetin C, ε-viniferin, and gnemonoside A/B. These compounds displayed similar physiochemical properties to lisinopril (ACEinhibitor), as in silico molecular docking studies demonstrated that they fit into the lisinopril receptors.Conclusion: In vitro analysis ethyl acetate extract from melinjo seeds demonstrated the highest ACE inhibitory activity. Molecular docking analysisindicated that resveratrol dimers, gnetin C and gnemonoside A can be considered ACE inhibitor.Keywords: Angiotensin converting enzyme inhibitor, Gnetum gnemon, Melinjo, Total phenolic, Antihypertension, Molecular docking.

scholarly journals In vivo hypotensive effect and in vitro inhibitory activity of some Cyperaceae species

2013 ◽  
Vol 49 (4) ◽  
pp. 803-809
Author(s):  
Monica Lacerda Lopes Martins ◽  
Henrique Poltronieri Pacheco ◽  
Iara Giuberti Perini ◽  
Dominik Lenz ◽  
Tadeu Uggere de Andrade ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Colorimetric Assay ◽  
Hypotensive Effect ◽  
Ace Inhibition ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Total Phenolic ◽  
Before And After

In 1820, French naturalist August Saint Hillaire, during a visit in Espírito Santo (ES), a state in southeastern Brazil, reported a popular use of Cyperaceae species as antidote to snake bites. The plant may even have a hypotensive effect, though it was never properly researched. The in vitro inhibitory of the angiotensin converting enzyme (ACE) activity of eigth ethanolic extracts of Cyperaceae was evaluated by colorimetric assay. Total phenolic and flavonoids were determined using colorimetric assay. The hypotensive effect of the active specie (Rhychonospora exaltata, ERE) and the in vivo ACE assay was measured in vivo using male Wistar Kyoto (ERE, 0.01-100mg/kg), with acetylcholine (ACh) as positive control (5 µg/kg, i.v.). The evaluation of ACE in vivo inhibitory effect was performed comparing the mean arterial pressure before and after ERE (10 mg/kg) in animals which received injection of angiotensin I (ANG I; 0,03, 03 and 300 µg/kg, i.v.). Captopril (30 mg/kg) was used as positive control. Bulbostylis capillaris (86.89 ± 15.20%) and ERE (74.89 ± 11.95%, ERE) were considered active in the in vitro ACE inhibition assay, at 100 µg/mL concentration. ACh lead to a hypotensive effect before and after ERE's curve (-40±5% and -41±3%). ERE showed a dose-dependent hypotensive effect and a in vivo ACE inhibitory effect. Cyperaceae species showed an inhibitory activity of ACE, in vitro, as well as high content of total phenolic and flavonoids. ERE exhibited an inhibitory effect on both in vitro and in vivo ACE. The selection of species used in popular medicine as antidotes, along with the in vitro assay of ACE inhibition, might be a biomonitoring method for the screening of new medicinal plants with hypotensive properties.

scholarly journals Total phenolic content and antioxidant capacity of hydromethanolic extracts of in vitro and ex vitro plantlets of Drosera spp

2020 ◽  
Vol 5 (3) ◽  
pp. 17-32
Author(s):  
Ana Karina Carrera-Felipe ◽  
◽  
Sugey Vásquez-Hernández ◽  
María Teresa González-Arnao ◽  
Enrique Bonilla-Zavaleta ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Ex Vitro

scholarly journals Preliminary Screening of Corn Silk (Zea mays L.) for Antioxidant Properties and Cytotoxicity Activity on Human Myelogenous Leukemia Cell Line

2020 ◽  
Vol 2 (1) ◽  
pp. 52
Keyword(s):  
Ethyl Acetate ◽  
Mtt Assay ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Myelogenous Leukemia ◽  
Leukemia Cell Line ◽  
Total Phenolic ◽  
Cytotoxicity Activity ◽  
Corn Silk ◽  
Ic50 Value

One of the traditionally used herbs is the byproduct of the maize plant, the 10-20 cm long corn silk which from the female maize flowers. The aim of this study is to evaluate the total phenolic content, antioxidant activity, and cytotoxicity activity of corn silk. The corn silk was minced and was extracted with methanol-water (80 % v/v), methanol, ethanol, ethyl acetate, and hexane by using the maceration method. The total phenolic content (TPC) of corn silk was determined to assess the presence and level of phenolic compounds in each sample. The antioxidant activities of all corn silk extracts were determined via DPPH method, and MTT assay was used to study the viability of the cells after the cells were treated with corn silk extracts at different time intervals. The highest phenolic content was exhibited by the methanol extract. The EC50 value for methanol-water (80 % v/v), methanol, ethanol, ethyl acetate and hexane extracts were 251 μg/ ml, 300 μg/ ml, 330 μg/ ml, 550 μg/ ml and 1736 μg/ ml respectively. The MTT assay, the lowest IC50 values at 24 and 48 hours intervals, was exhibited by methanol-water extract (104 μg/ ml). In contrast, methanol (308 μg/ ml) was found with the highest IC50 value for all 24, 48, and 72 hours intervals. At 72 hours interval, ethyl acetate (88 μg/ ml) shown the lowest IC50 value. This study suggested that corn silk could be potentially used as a source of antioxidant and can further evaluate for cancer studies.

Sign in / Sign up

Export Citation Format

Share Document