scholarly journals Optimization of xylanase production by Cryptococcus flavescens LEB-AY10 from steam exploded sugarcane bagasse

2015 ◽  
Vol 3 (2) ◽  
pp. 8-17 ◽  
Author(s):  
Andrade CCP ◽  
Santos TP ◽  
Franco SF ◽  
Rodrigues MI ◽  
Pereira GAG ◽  
...  
Keyword(s):  
Sugarcane Bagasse ◽  
Xylanase Production ◽  
Cryptococcus Flavescens

Xylanase production by Cryptococcus flavescens and Aureobasidium pullulans using steam exploded bagasse

2012 ◽  
Vol 29 ◽  
pp. S200
Author(s):  
Cristiane Conte Paim de Andrade ◽  
Tatiana Porto dos Santos ◽  
Maria Isabel Rodrigues ◽  
Francisco Maugeri Filho
Keyword(s):  
Aureobasidium Pullulans ◽  
Xylanase Production ◽  
Cryptococcus Flavescens

scholarly journals Combined pretreatment of sugarcane bagasse using alkali and ionic liquid to increase hemicellulose content and xylanase production

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Rozina Rashid ◽  
Uroosa Ejaz ◽  
Firdous Imran Ali ◽  
Imran Ali Hashmi ◽  
Ahmed Bari ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Sugarcane Bagasse ◽  
Fermentation Medium ◽  
Chemical Pretreatment ◽  
Chemical Methods ◽  
Xylanase Production ◽  
Box Behnken Design ◽  
Combined Pretreatment ◽  
Scanning Electron ◽  
Better Than

Abstract Background Lignin in sugarcane bagasse (SB) hinders its utilization by microorganism, therefore, pretreatment methods are employed to make fermentable components accessible to the microbes. Multivariate analysis of different chemical pretreatment methods can aid to select the most appropriate strategy to valorize a particular biomass. Results Amongst methods tested, the pretreatment by using sodium hydroxide in combination with methyltrioctylammonium chloride, an ionic liquid, (NaOH+IL) was the most significant for xylanase production by Bacillus aestuarii UE25. Investigation of optimal levels of five significant variables by adopting Box-Behnken design (BBD) predicted 20 IU mL− 1 of xylanase and experimentally, a titer of 17.77 IU mL− 1 was obtained which indicated the validity of the model. The production kinetics showed that volumetric productivity of xylanase was much higher after 24 h (833.33 IU L− 1 h− 1) than after 48 h (567.08 IU L− 1 h− 1). The extracted xylan from SB induced more xylanase in the fermentation medium than pretreated SB or commercially purified xylan. Nuclear Magnetic Resonance, Fourier transform infrared spectroscopy and scanning electron microscopy of SB indicated removal of lignin and changes in the structure of SB after NaOH+IL pretreatment and fermentation. Conclusion Combined pretreatment of SB with alkali and methyltrioctylammonium chloride appeared better than other chemical methods for bacterial xylanase production and for the extraction of xylan form SB.

scholarly journals Xylanase production by Trichoderma harzianum rifai by solid state fermentation on sugarcane bagasse

2002 ◽  
Vol 33 (1) ◽  
Author(s):  
Maria Inês Rezende ◽  
Aneli de Melo Barbosa ◽  
Ana Flora Dalberto Vasconcelos ◽  
Asae Sakurada Endo
Keyword(s):  
Solid State ◽  
Sugarcane Bagasse ◽  
Solid State Fermentation ◽  
Trichoderma Harzianum ◽  
Xylanase Production

Raw sugarcane bagasse as carbon source for xylanase production by Paenibacillus species: a potential degrader of agricultural wastes

2017 ◽  
Vol 24 (23) ◽  
pp. 19057-19067 ◽  
Author(s):  
Enzo Di Marco ◽  
Pablo M. Soraire ◽  
Cintia M. Romero ◽  
Liliana B. Villegas ◽  
María Alejandra Martínez
Keyword(s):  
Carbon Source ◽  
Sugarcane Bagasse ◽  
Agricultural Wastes ◽  
Xylanase Production ◽  
Paenibacillus Species

scholarly journals Assessment of a novel pretreatment techniques for enhancing the enzymatic saccharification of sugarcane baggase: Structural and chemical analysis

2020 ◽  
Author(s):  
Soni Tiwari ◽  
Janardan Yadav ◽  
Rajeeva Gaur ◽  
Jay Shanker Yadav
Keyword(s):  
Sugarcane Bagasse ◽  
Enzymatic Reaction ◽  
Enzymatic Saccharification ◽  
Sem Analysis ◽  
Tween 20 ◽  
Acid Pretreatment ◽  
Xylanase Production ◽  
Structure Changes ◽  
Naoh Solution ◽  
Pretreated Sugarcane Bagasse

Abstract Background Enhancement of cellulase and xylanase production and improvement of more proficient lignocellulose-degrading enzymes are essential in order to decrease the price of enzymes required in the biomass-to-bioethanol production. Results The effectiveness of different concentration of alkali and acid pretreatment of sugarcane bagasse for improving the enzymatic saccharification of cellulose has been evaluated. The sugarcane bagasse was characterized to contain 39.52% cellulose, 25.63% hemicelluloses, 30.36% lignin, 1.44% ash and 2.90% other extractives. Afterthat, The sugarcane bagasse was pretreated with two different concentrations (5% and 10%) of H 2 SO 4 and NaOH at 121°C for 60 min. Among them, the best result was obtained when sugarcane bagasse was pretreated with 10% NaOH solution followed by 10% H 2 SO 4 , 5% NaOH and 5% H 2 SO 4 solution. The highest cellulose saccharification was 489.5 mg/g from 10% NaOH pretreatment followed by 322.75mg/g, 301.25 mg/g and 276.6 mg/g from 10% H 2 SO 4 , 5% NaOH and 5% H 2 SO 4 , respectively, which were 55.1, 32.0, 27.1 and 20.6 times higher than the control. Moreover, the FTIR, XRD and SEM analysis showed significant molecule and surface structure changes of the sugarcane bagasse after different pretreatments. Cellulase and xylanase produced by Pseudomonas sp. CVB-10 [MK443365] and Bacillus paramycoides T4 [MN370035] was used to hydrolyze the pretreated sugarcane bagasse and the optimal condition was determined to be 30 h of enzymatic reaction with 3:1 ration of enzymes under the temperature of 55°C, pH 5.5, substrate concentration of 3% and Tween-20 0.5%. Conclusion Enzyme supernatants produced by the mixed culture of Pseudomonas sp. CVB-10 [MK443365] and Bacillus paramycoides T4 [MN370035] on various pretreated sugarcane baggase have good cellulase and xylanase activities, leading to celluloses and Hemicelluloses conversion in the enzymatic hydrolysis/saccharification that is more proficient.

scholarly journals Evaluation of Pretreatment Methods (Acid and Alkali) in Improving the Enzymatic Saccharification of Sugarcane Baggase: Structural and Chemical Analysis

2020 ◽  
Author(s):  
Soni Tiwari ◽  
Janardan Yadav ◽  
Rajeeva Gaur ◽  
Jay Shanker Yadav
Keyword(s):  
Sugarcane Bagasse ◽  
Enzymatic Reaction ◽  
Enzymatic Saccharification ◽  
Sem Analysis ◽  
Tween 20 ◽  
Acid Pretreatment ◽  
Xylanase Production ◽  
Structure Changes ◽  
Naoh Solution ◽  
Pretreated Sugarcane Bagasse

Abstract Background: Enhancement of cellulase and xylanase production and improvement of more proficient lignocellulose-degrading enzymes are essential in order to decrease the price of enzymes required in the biomass-to-bioethanol production. Results: The effectiveness of different concentration of alkali and acid pretreatment of sugarcane bagasse for improving the enzymatic saccharification of cellulose has been evaluated. The sugarcane bagasse was characterized to contain 39.52% cellulose, 25.63% hemicelluloses, 30.36% lignin, 1.44% ash and 2.90% other extractives. Afterthat, The sugarcane bagasse was pretreated with two different concentrations (5% and 10%) of H2SO4 and NaOH at 121°C for 60 min. Among them, the best result was obtained when sugarcane bagasse was pretreated with 10% NaOH solution followed by 10% H2SO4, 5% NaOH and 5% H2SO4 solution. The highest cellulose saccharification was 489.5 mg/g from 10% NaOH pretreatment followed by 322.75mg/g, 301.25 mg/g and 276.6 mg/g from 10% H2SO4, 5% NaOH and 5% H2SO4, respectively, which were 55.1, 32.0, 27.1 and 20.6 times higher than the control. Moreover, the FTIR, XRD and SEM analysis showed significant molecule and surface structure changes of the sugarcane bagasse after different pretreatments. Cellulase and xylanase produced by Pseudomonas sp. CVB-10 [MK443365] and Bacillus paramycoides T4 [MN370035] was used to hydrolyze the pretreated sugarcane bagasse and the optimal condition was determined to be 30 h of enzymatic reaction with 3:1 ration of enzymes under the temperature of 55°C, pH 5.5, substrate concentration of 3% and Tween-20 0.5%. Conclusion: Enzyme supernatants produced by the mixed culture of Pseudomonas sp. CVB-10 [MK443365] and Bacillus paramycoides T4 [MN370035] on various pretreated sugarcane baggase have good cellulase and xylanase activities, leading to celluloses and Hemicelluloses conversion in the enzymatic hydrolysis/saccharification that is more proficient.

Xylanase production by endophyticAspergillus nigerusing pentose-rich hydrothermal liquor from sugarcane bagasse

2015 ◽  
Vol 33 (3) ◽  
pp. 175-187 ◽  
Author(s):  
Diogo Robl ◽  
Priscila da Silva Delabona ◽  
Patrícia dos Santos Costa ◽  
Deise Juliana da Silva Lima ◽  
Sarita Candida Rabelo ◽  
...  
Keyword(s):  
Sugarcane Bagasse ◽  
Xylanase Production

scholarly journals Xylanase production by Streptomyces viridosporus T7A in submerged and solid-state fermentation using agro-industrial residues

2009 ◽  
Vol 52 (spe) ◽  
pp. 171-180 ◽  
Author(s):  
Luiz Romulo Alberton ◽  
Luciana Porto de Souza Vandenberghe ◽  
Ricardo Assmann ◽  
Ricardo Cancio Fendrich ◽  
José Rodriguéz-León ◽  
...  
Keyword(s):  
Solid State ◽  
Sugarcane Bagasse ◽  
Solid State Fermentation ◽  
Xylanase Activity ◽  
Napier Grass ◽  
Xylanase Production ◽  
Industrial Residues ◽  
Streptomyces Viridosporus ◽  
Substrate Support ◽  
B Complex Vitamins

The study of xylanase production was conducted by Streptomyces viridosporus T7A in submerged (SmF) and solid-state fermentation (SSF), using agro-industrial residues and sub-products. Napier grass, sugarcane bagasse and soybean bran were used as carbon source, substrate/support, and nitrogen source, respectively. In SmF, Napier grass (1% v/w) supplemented with soybean bran, hydroxyethylcellulose and B complex vitamins were used. Soybean bran (1.5 % w/v), B complex vitamins (0.1%), and hydroxyethilcellulose (0.15%) led to an increase in xylanase production (23.41 U/mL). In SSF, the effects of the following parameters were studied: substrate composition (sugarcane bagasse, Napier grass and soybean bran), initial moisture, and inoculum rate. In SSF, the highest xylanase activity (423.9 U/g) was reached with: 70 % sugarcane bagasse, 20% Napier grass and 10% soybean meal, 90% of moisture, and 10(7)/g substrate.

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