scholarly journals Immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation

2013 ◽  
Vol 16 (4) ◽  
pp. 38-43
Author(s):  
Alexandra Evgen'evna Proshchina ◽  
Yulia Sergeevna Krivova ◽  
Valeriy Mikhaylovich Barabanov ◽  
Sergey Vyacheslavovich Savelyev
Keyword(s):  
Diabetes Mellitus ◽  
Pancreatic Islets ◽  
Age Groups ◽  
Diabetes Mellitus Type 1 ◽  
Pancreatic Tissue ◽  
Control Group ◽  
Human Pancreas ◽  
Patients With Diabetes ◽  
The Rich

Aim.  To study the distribution and cellular architecture of the largest human pancreatic islets (with a diameter of more than 200 micron) in aging and diabetes mellitus types 1 and 2. Materials and methods.  Antibodies to insulin, glucagon, somastatin and nturon-specific enolase were applied. The autopsy samples of the pancreatic tissue of patients with diabetes mellitus type 1 (DMT1) and type 2 (DMT2) and 2 age groups (up to 50 years old (control) and after 50 (aging control)), not suffering from diseases of the pancreas and carbohydrate metabolism malfunction were investigated. Results.  The number of islets with diameter more than 200 mkm compared to control group increased both in aging and diabetes groups. Their number reaches in some cases 15% (and higher in DM) of the total number of islets. These islets compared to the other are rich-vascularized. It was shown that glucagon and somatostatin-containing cells are found both on the periphery of the large islets, and inside them only in the immediate proximity of the capillaries. Insulin-containing cells form clusters, surrounded by the capillaries and the ?- and ?-cells, while the inner part of such clusters has no direct contact with the capillaries. In the large islets the number of glucagon-containing cells is often increased, and insulin-containing cells show signs of degradation. Conclusion.  The largest of the pancreatic islets may be useless for the transplantation, because of the high content of glucagon-containing cells, the rich vascularization and, in some cases, the limited functionality of ?-cells.

scholarly journals Renal dysfunction markers in patients with diabetes mellitus type 1 after kidney or simultaneous kidney-pancreas transplantation

2016 ◽  
Vol 62 (5) ◽  
pp. 14-16
Author(s):  
Alexandra M. Glazunova ◽  
Larisa V. Nikankina ◽  
Alexandr V. Ilin ◽  
Minara S. Shamkhalova ◽  
Gulya M. Musaeva ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Renal Transplant ◽  
Renal Dysfunction ◽  
Cystatin C ◽  
Pancreas Transplantation ◽  
Diabetes Mellitus Type 1 ◽  
Diabetes Mellitus Type ◽  
Control Group ◽  
Patients With Diabetes

Objective. To examine kidney transplant dysfunction markers in patients with diabetes mellitus type 1 (T1DM) after kidney transplantation (KT) and simultaneous kidney-pancreas transplantation (SPK).Materials and methods. The study included 20 patients after successful SPK (group 1) and 41 patients after KT (21 received insulin pump therapy (group 2), 20 –multiple daily injections of insulin (group 3). Post transplantation period at the time of inclusion in the KT group was 8 months [7;8], in SPK-11 months [8;18]. The control group consisted of 15 patients with DM1 without diabetic nephropathy (group 4). Sex, age and duration of T1DM were comparable. Donors of SPK were younger than KT: 29 [25; 33] vs 46[30; 51] years p<0,01 and transplant cold ischemia time was less 8[7;10] vs 11,5 [1; 17] hours respectively, p<0,01. After 9 months of observation biomarkers of dysfunction of renal transplant: Cystatin C (serum, urine); NGAL, KIM-1, podocin, nephrin, IL-18, IP-10 (urine), TGF-β1, MMP-9, VEGF-A, Osteopontin – (OPN) (serum) were defined.Results. the level of GFR in patients after transplantation was C2 stage, albuminuria A1 of chronic kidney disease. In the group of patients with T1DM after successful SPK and KT revealed a significant increase in markers of renal dysfunction (cystatin C (serum), NGAL, Podocin, OPN) compared with the control group despite of carbohydrate metabolism compensation (Tabl.1). High level and a negative associated of blood cystatin C with GFR (r = - 0,36, p<0.05) and positive with albuminuria (r=0,40, p<0,05), as well as a direct link of podocin urine-with blood creatinine (r = 0,35, p<0.05) and NGAL with albuminuria (r = 0,35, p<0.05) in recipients after transplantation were defined. Association between podocin with MMP-9 (r = 0,46, p<0,05) and NGAL (r = 0,33, p<0,05) indicated correlation of stress factors of renal microstructures in posttransplantation patients.Conclusion. High levels of renal graft dysfunction biomarkers in the examined patients (including those after SPK) show the persistence of damage to the microstructures with stable graft function and demonstrate the need to control all factors in the preservation of renal function.Table 1. Renal transplant dysfunction markersParametrsGroup 1Group 2Group 3Group 4TGF b1 (serum, pg/ml)32999[24514;3917]24473[21752;33330]25139[11367;2862]26986[17347;4266]VEGF A (serum, pg/ml)471,9[296;530,6]#407,6[301,6;522,2] #226,6[177,8;367,4]467,4[288,3;474,8]CYS C, (serum, ng/ml)1047[985;1295]*∞1252,9[1151;1540]#∞1113,32[986;1257] §728,8[592,9;765,3]Osteopontin (serum, ng/ml)3,51[2,7;4,9] #∞4,28[2,8;8,2] ∞4,71[3,6;12,7] §2,86[2,2;3,1]MMP-9 (urine, ng/ml)1,15[1,1;1,7]1,30[1,2;1,9] #1,10[0,9;1,3]1,22[1,0;1,3]IP-10 (urine, ng/ml)17,83[17,32;18,36]17,83[17,32;18,36]18,36[17,83;18,90]18,36[17,83;18,90]CYS C (urine, ng/ml)10407[5812;16306]15574[7518;28397]13329[7006;24624]14701[3643;26666]Podocin (urine, ng/ml)0,41[0,18;0,51] #0,49[0,26;0,69]0,56[0,38;0,79]§0,36[0,1;0,51]Nephrin (urine, ng/ml)0,0[0,0;0,1]0,0[0,0;01]0,0[0,0;0,07]0,07[0,0;0,1]KIM-1 (urine, ng/ml)211,8[83,3;368,4]314,9[152,1;508,6]338,7[191,3;594,0]359,2[204,4;494,5]NGAL (urine, ng/ml)2,4[1,7;6,7] *7,8[2,8;14,5] ∞2,9[1,8;12,0]§2,3[1,7;7,3]* р<0,01 (1-2); # р<0,01 (1,2-3); ∞ р<0,01 (1,2-4); § р<0,01 (3–4)

Prevalence of Fungal Foot Infections in Patients with Diabetes Mellitus Type 1 - Underestimation of Moccasin-Type Tinea

2004 ◽  
Vol 112 (05) ◽  
pp. 264-268 ◽  
Author(s):  
P. Mayser ◽  
J. Hensel ◽  
W. Thoma ◽  
M. Podobinska ◽  
M. Geiger ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Diabetes Mellitus Type 1 ◽  
Diabetes Mellitus Type ◽  
Patients With Diabetes

scholarly journals Interferência nutricional no tratamento de pacientes com Diabetes Mellitus tipo 1/ Nutritional interference in the treatment of patients with Diabetes Mellitus type 1

2018 ◽  
Vol 17 (3) ◽  
Author(s):  
Daniela Cristina Silva Resende ◽  
Adriana Paula da Silva ◽  
Janaíne Machado Tomé ◽  
Elisabete Aparecida Mantovani Rodrigues de Resende ◽  
Heloísa Marcelina Cunha Palhares ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Diabetes Mellitus Type 1 ◽  
Diabetes Mellitus Type ◽  
Patients With Diabetes

O tratamento do Diabetes Mellitus tipo 1 (DM1) constitui-se na adesão ao tratamento insulínico, na alimentação e na atividade física, visando ao controle glicêmico. O objetivo deste estudo foi observar os efeitos da interferência nutricional no tratamento de pacientes com DM1. Trata-se de estudo quantitativo, prospectivo e longitudinal desenvolvido no Ambulatório de Diabetes da UFTM. A coleta de dados foi realizada entre março de 2013 e setembro de 2014. Foram avaliados 41 crianças e adolescentes entre 6 e 17 anos, quanto à antropometria, controle glicêmico e lipídico em 4 momentos: M1 no início do seguimento; M2 após orientação nutricional convencional; M3 após aprendizagem da contagem de carboidratos (CCHO) e M4 em contagem plena. A análise estatística foi descritiva e inferencial. A antropometria comprovou que a CCHO não resultou em ganho de peso e foi efetiva no sexo masculino, demonstrada pela redução nas concentrações de frutosamina (p=0,050) e HbA1C (p=0,041) no M4 comparado ao M1. Considerando a frutosamina, o grupo com CCHO se diferenciou do grupo sem CCHO M4 (p=0,035). A terapêutica insulínica associada à CCHO demonstrou ser um recurso importante a ser integrado no tratamento do DM1, visando atingir alvos efetivos na redução das complicações.

scholarly journals Eye and renal complication in pediatric patients with diabetes mellitus type 1

2015 ◽  
Vol 2015 (S1) ◽  
Author(s):  
Nguyen Tran Ngoc Hieu ◽  
Bui PhuongThao ◽  
Vu Chi Dung ◽  
Nguyen Ngoc Khanh ◽  
Can Thi Bich Ngoc ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Pediatric Patients ◽  
Diabetes Mellitus Type 1 ◽  
Diabetes Mellitus Type ◽  
Renal Complication ◽  
Patients With Diabetes
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