scholarly journals Aktivitas Antibakteri Ekstrak Metanol Rumput Laut Gracilaria verrucosa, Greville, 1830 (Florideophyceae : Gracilariaceae) di Balai Besar Perikanan Budidaya Air Payau Jepara

2019 ◽  
Vol 8 (3) ◽  
pp. 285-290
Author(s):  
Radhian Wikanarto Widodo ◽  
Subagiyo Subagiyo ◽  
Rini Pramesti
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Staphylococcus Epidermidis ◽  
Methanol Extract ◽  
Vibrio Anguillarum ◽  
Vibrio Alginolyticus ◽  
World Health ◽  
Brackish Water Aquaculture

Infeksi bakteri patogen menjadi masalah kesehatan dunia. Antibiotik untuk penanggulangan infeksi menjadikan bakteri resisten karena pemakaiannya tidak sesuai dosis. Permasalahan ini dapat diatasi salah satunya dengan pencarian sumber antibiotik baru termasuk diantaranya pada rumput laut. Penelitian ini bertujuan untuk menentukan aktivitas antibakteri ekstrak metanol G. verrucosa terhadap Escherichia coli, Bacillus cereus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Vibrio anguillarum, dan Vibrio alginolyticus. Penelitian dilakukan dengan metode eksperimental laboratoris. Sampel rumput laut hasil budidaya Balai Besar Perikanan Budidaya Air Payau (BBPBAP) Jepara dikeringkan kemudian dimaserasi dengan metanol selama 2x24 jam. Aktivitas antibakteri ekstrak diuji pada konsentrasi 100 µg/disk, 50 µg/disk, 25 µg/disk, dan 10 µg/disk kemudian dilakukan uji fitokimia. Hasil uji aktivitas antibakteri menunjukkan bahwa ekstrak metanol G. verrucosa tidak memiliki aktivitas antibakteri. Uji fitokimia menunjukkan ekstrak mengandung flavonoid, saponin, steroid, dan triterpenoid. Pathogenic bacterial infection become a world health problem. Antibiotics for prevention of infection make bacteria resistant due to the incorrect dosage. One of these problems can be overcome by finding a new source of antibiotics including seaweed. This study aims to determine the antibacterial activity of methanol extract of G. verrucosa against Escherichia coli, Bacillus cereus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Vibrio anguillarum, and Vibrio alginolyticus. Laboratory experimental method was used in this study. Seaweed samples from the cultivation at Brackish Water Aquaculture Center (BBPBAP) Jepara were dried and then macerated with methanol for 2x24 hours. The antibacterial activity of the extract was tested at concentration of 100 µg / disk, 50 µg / disk, 25 µg / disk, and 10 µg / disk then phytochemical tests were carried out. The results of antibacterial activity test showed that G. verrucosa methanol extract did not have antibacterial activity. The phytochemical test showed that seaweed extract contained flavonoids, saponins, steroids, and triterpenoids

scholarly journals IN VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY OF MICRO PROPAGATED CALLUS OF CURUULIGO ORCHIODES (BLACK MUSILLI) AGAR WELL DIFFUSION AND MINIMUM INHIBITORY CONCENTRATION (MIC)

2017 ◽  
Vol 9 (3) ◽  
pp. 75
Author(s):  
Rajanikanth Garapati ◽  
N. Ramesh
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Bacillus Cereus ◽  
Proteus Vulgaris ◽  
Methanol Extracts ◽  
Tract Infections

Objective: In vitro investigated the potential of methanol extracts of micro-propagated C. orchiodes in the antimicrobial property against the three gram-negative bacteria, two gram-positive and one fungal filament.Methods: The micro propagated callus methanol extract was examined against Escherichia coli, Proteus vulgaris, Salmonella typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus and Candida albicans. The zone of inhibitions are determined at 10 mg/ml concentration of methanol extracts of callus on agar well plate and MIC against tested microorganism.Results: The highest antibacterial activity recorded in Staphylococcus aureus Bacillus cereus and followed by Candida albicans. Antibacterial activity of leaf extracts of A. reticulata was also significant against the tested microorganisms Escherichia coli, Salmonella typhi, Proteus vulgaris, Pseudomonas aeruginosa compared to ciprofloxacin.Conclusion: Based on the above observations, these extracts were further evaluated for their effect on microorganisms causing infections like typhoid fever, urinary tract infections, septicemia, toxic shock syndrome, skin infection, nosocomial infection, arthritis and diarrhoea. The results also suggest that these plants serve a therapeutic purpose in the treatment bacterial infections.

Antibacterial Activity of Coumarins

2005 ◽  
Vol 60 (9-10) ◽  
pp. 693-700 ◽  
Author(s):  
Simone M. de Souza ◽  
Franco Delle Monache ◽  
Artur Smânia
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Coumarin Derivatives ◽  
Inhibitory Effects ◽  
Gram Positive Bacteria ◽  
Per Se ◽  
And Staphylococcus Aureus

Abstract The antibacterial activity of coumarin per se and other 45 coumarin derivatives was tested against strains of Bacillus cereus MIP 96016, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 25923. The inhibitory effects of coumarins were affected by their substitution patterns. Osthenol (44) showed the most effective antibacterial activity against Gram-positive bacteria with MIC values ranging between 125 and 62.5 μg/ml. These results suggested that the prenyl chain of 44 at position 8 and the presence of OH at position 7 of the benzenic ring are required for the antibacterial activity against these strains.

scholarly journals Aktivitas Antibakteri Minyak Atsiri Kulit Kayu Akway (Drimys piperita Hook. f.) pada Beberapa Tingkat Konsentrasi, Keasaman (pH) dan Kandungan Garam

2019 ◽  
Vol 8 (4) ◽  
pp. 149
Author(s):  
Gino Nemesio Cepeda ◽  
Meike Meilan Lisangan ◽  
Isak Silamba
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Sodium Chloride ◽  
Essential Oil ◽  
Bacillus Cereus ◽  
Chloride Content ◽  
Antibacterial Activities ◽  
Water Distillation

Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri minyak atsiri kulit kayu akway pada beberapa tingkat konsentrasi, keasaman (pH) dan kandungan sodium klorida. Minyak atsiri disuling dengan menggunakan metode distilasi air. Pengujian aktivitas antibakteri minyak atsiri pada beberapa tingkat konsentrasi, pH dan kandungan sodium klorida dilakukan dengan menggunakan metode difusi sumur. Hasil penelitian menunjukkan bahwa aktivitas antibakteri minyak atsiri kulit kayu akway cenderung meningkat dengan meningkatnya konsentrasi. Konsentrasi penghambatan minimum terhadap Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa dan Staphylococcus aureus adalah 0,28–0,56%. Tingkat keasaman dan kandungan sodium klorida tidak berpengaruh nyata terhadap aktivitas antibakteri minyak atsiri kulit kayu akway. Kesimpulannya, minyak atsiri kulit kayu akway berpotensi sebagai sumber antibakteri alami untuk menghambat pertumbuhan bakteri yang tahan terhadap antibiotik.Antibacterial Activity of Essential Oil of Akway (Drimys piperita Hook f.) Barks on Some Levels of Concentration, Acidity (pH) and Salt ContentsAbstractAkway (Drimys piperita Hook. f) was an aromatic plant of winteraceae. Leaves and barks of this plant contain essential oil. Previous studies indicated that essential oil from some aromatic plants had strong antibacterial activities. The aims of the study were to know antibacterial activities of essential oil isolated from akway bark on some levels of concentration, acidity (pH) and sodium chloride content. The essential oil was distilled by using water distillation method. The antibacterial activity was assayed on several levels of concentration, pH and sodium chloride concentrations that were performed using method of agar well diffusion. The results showed that the antibacterial activity of akway barks essential oil tended to increase with increasing of concentrations. The minimum inhibition concentrations against Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, and Staphylococcus aureus were 0,28–0,56%. The pH and sodium chloride contents had not significantly influenced to the antibacterial activities of akway barks essential oil. As conclusion, the essential oil of akway barks had potential as source of antibacterial on inhibiting growth of antibiotic resistance bacteria. 

Two Epimeric Flavalignans from Trichilia catigua (Meliaceae) with Antimicrobial Activity

2002 ◽  
Vol 57 (5-6) ◽  
pp. 483-488 ◽  
Author(s):  
Moacir G. Pizzolatti ◽  
Andreia F. Venson ◽  
Artur Smânia Júnior ◽  
Elza de F. A. Smânia ◽  
Raimundo Braz-Filho
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Natural Products ◽  
Bacillus Cereus ◽  
Spectroscopic Data ◽  
2D Nmr ◽  
And Staphylococcus Aureus

A mixture of flavalignan cinchonains Ia and Ib was isolated from the bark of Trichilia catigua. The structures were established on the basis of spectroscopic data of the natural products and their methylated derivatives including 2D NMR experiments, and compared with data in the literature. These flavalignans exhibited antibacterial activity against Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus.

scholarly journals KHẢO SÁT ĐIỀU KIỆN NUÔI CẤY ĐỂ NÂNG CAO KHẢ NĂNG KHÁNG KHUẨN CỦA CÁC CHỦNG Streptomyces sp.

2021 ◽  
Vol 5 (1) ◽  
pp. 2227-2236
Author(s):  
Nguyễn Thị Thủy Tiên ◽  
Lê Thị Thu Hiền ◽  
Trần Thị Lệ Ngân ◽  
Trần Văn Trung ◽  
Nguyễn Thị Thúc ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
16S Rrna ◽  
Bacillus Cereus ◽  
Salmonella Typhi ◽  
Streptomyces Sp ◽  
International Streptomyces Project ◽  
E Coli

Streptomyces là những vi sinh vật có khả năng sinh tổng hợp các chất kháng sinh. Nghiên cứu này nhằm sàng lọc và chọn chủng có khả năng kháng khuẩn cao nhất từ 59 chủng Streptomyces có nguồn gốc từ đất đã được cung cấp dựa trên phương pháp cấy vạch vuông góc đối với 5 vi khuẩn gây bệnh chỉ thị, bao gồm Bacillus cereus, Escherichia coli, Salmonella typhi, Staphylococcus aureus và Pseudomonas aeruginosa. Mười lăm chủng thể hiện hoạt tính kháng khuẩn ở các mức độ khác nhau, các chủng còn lại không thể hiện khả năng kháng. Chủng có khả năng kháng khuẩn cao nhất là HĐM3.2, kháng 4/5 vi khuẩn chỉ thị đã sử dụng, gồm B. cerius, S. aureus, S. typhi và E. coli với kích thước vùng kháng đạt 9,5, 10,5, 16,5 và 14,5 mm. Chủng HĐM3.2 được xác định là chủng Streptomyces sp. dựa trên cây phát sinh loài đã xây dựng của trình tự gene 16S rRNA. Điều kiện nuôi cấy để chủng HĐM3.2 thể hiện hoạt tính kháng khuẩn cao nhất đã được khảo sát dựa trên phương pháp khuếch tán qua giếng thạch. Môi trường International Streptomyces Project 2 có pH 8, nhiệt độ nuôi cấy 28oC là điều kiện thích hợp để chủng Streptomyces sp. HĐM3.2 tạo ra vùng ức chế các loại vi khuẩn B. cereus, E. coli, S. typhi, S. aureus cao nhất. ABSTRACT Streptomyces is the microorganism that has capable of producing antibiotics. The current study aimed to screen and to select a strain that had the highest antibacterial activity from 59 available soil-derived Streptomyces strains based on the perpendicular culture method on 5 indicator pathogenic bacteria, including Bacillus cereus, Escherichia coli, Salmonella typhi, Staphylococcus aureus và Pseudomonas aeruginosa. Fourteen strains exhibited their antibacterial activity at various levels, the remaining did not have that activity. The strain that had the highest antibacterial ability was HĐM3.2 against 4/5 indicator microorganisms, including B. cerius, S. aureus, S. typhi and E. coli with inhibitory areas were 9.5, 10.5, 16.5, and 14.5 mm respectively. Strain HĐM3.2 was identified as strain Streptomyces sp. based on a phylogenetic tree built on the 16S rRNA gene sequences. Suitable conditions that made the strain HĐM3.2 showed the highest antibacterial activity were investigated based on agar well diffusion assay. The medium of International Streptomyces Project 2 with pH 8, the ambient temperature at 28oC were suitable conditions for Streptomyces sp. HĐM3.2 produced the highest inhibitory areas against B. cereus, E. coli, S. typhi, S. aureus.  

scholarly journals INVESTIGAÇÃO FITOQUÍMICA E AVALIAÇÃO DA ATIVIDADE ANTIBACTERIANA DA Mikania lanuginosa DC (ASTERACEAE)

2002 ◽  
Vol 3 (2) ◽  
Author(s):  
R. Z. Da Silva ◽  
E. De M. RIOS ◽  
M. Z. Da SILVA ◽  
L. De F. Leal ◽  
R. A. YUNES ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Cereus ◽  
Staphylococcus Epidermidis ◽  
Dilution Method ◽  
Isolation And Purification ◽  
Kaurenoic Acid ◽  
Reported Data

O presente trabalho envolveu extração, isolamento, purificação e identificação dos constituintes químicos das partes aéreas da Mikania lanuginosa DC (ASTERACEAE). No processo de extração ocorreu a formação de um precipitado, no qual foi isolado e purificado um composto majoritário, que por análises espectroscópicas de IV, RMN 1H, RMN 13C e por comparação com os dados existentes na literatura, foi identificado como sendo o diterpeno ácido caurenóico. Foram realizados ensaios biológicos para avaliar a ação antimicrobiana com o precipitado, extrato etanólico bruto, as frações hexânica, diclorometano, acetato de etila, butanólica e o diterpeno ácido caurenóico contra as cepas Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228 e Bacillus cereus, pela técnica da difusão em meio sólido por cavidade e posteriormente pelo método de diluição em placa. Os resultados demonstraram que as frações hexânica, diclorometano, acetato de etila, butanólica e o ácido caurenóico obtidos da M. lanuginosa, apresentaram atividade antimicrobiana moderada frente as bactérias Gram positivas e foram inativos contra as bactérias Gram negativas. Phytochemical Investigation and Antimicrobial Activity Evaluation of the Mikania lanuginosa DC (ASTERACEAE) Abstract The present study investigated the extraction, isolation and purification the chemical compounds of the aerial parts of Mikania lanuginosa DC (ASTERACEAE). In the process of extraction it occurred the formation of a precipitate, it which was isolated and purified a ruling compound, and by spectroscopic analysis of the IR, 1H NMR, 13C NMR and comparison with reported data in the literature it was identified as being the diterpene kaurenoic acid. It were performed biological assays to estimate the antimicrobial action with the precipited, crude etanolic extract, hexane, dichoromethane, acetate ethyl, butane fractions and diterpene kaurenoic acid contrasting with strains Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228 e Bacillus cereus, by technique of the diffusion in solid mean by cavity and afterwards by the plaque dilution method. The results showed that the hexane, dichoromethane, acetate ethyl, butane fractions and the kaurenoic acid obtained from the Mikania lanuginosa DC presented a moderate antimicrobial activity face to Gram positives bacteria and got inert against the Gram negatives bacteria.

scholarly journals SKIRTINGŲ SUDĖČIŲ KONTAKTINIŲ LĘŠIŲ TIRPALŲ ANTIMIKROBINĖS SAVYBĖS

2019 ◽  
Vol 28 (6) ◽  
pp. 74-80
Author(s):  
Žaneta Maželienė ◽  
Ieva Dumšytė ◽  
Jolita Kirvaitienė ◽  
Ingrida Viliušienė
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Bacillus Cereus ◽  
Staphylococcus Epidermidis

Įvadas. Akies infekcijos rizika dažnėja didėjant kon­taktinių lęšių nešiotojų skaičiui. Kontaktinių lęšių tirpalai – priemonės, skirtos kontaktinių lęšių skala­vimui, nešvarumų valymui, apnašų šalinimui, dezin­fekcijai (mikroorganizmų sunaikinimui), drėkinimui bei laikymui. Kontaktinių lęšių tirpalų sudėtis nule­mia skirtingas jų antimikrobines savybės. Tyrimo tikslas. Nustatyti kontaktinių lęšių skysčių antimikrobinį veikimą skirtinguose jų poveikio eta­loninėms bakterijų kultūroms laiko intervaluose. Tyrimo medžiaga ir metodai. Mikrobiologinio tyrimo metu nustatytas kontaktinių lęšių tirpalų antimikro­binis poveikis bakterijoms – Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 27853, Bacillus cereus ATCC 11778. Bakte­rijų kultūros buvo veikiamos skirtingais kontaktinių lęšių tirpalais 0,5 val., 1 val., 2 val., 4 val., 6 val., 8 val. ir po to nustatytas likęs gyvybingų bakterijų kie­kis tiriamajame tirpale. Rezultatai. Kontaktinių lęšių tirpalų antimikrobines savybes lemia kelių sudedamųjų dalių sinergistinis antimikrobinis poveikis dažniausiai akims pavojin­giems infekcinių ligų sukėlėjams. Silpniausiai bak­terijas veikė poliheksametilenbiguanido 0,0001 proc. tirpalas. Poliheksametilenbiguanido tirpalo antimi­krobinis poveikis stiprėjo didėjant jo koncentraci­jai ir į tirpalą pridėjus ramunėlių ar alavijo. Polihek­sametilenbiguanido 0,0002 proc. tirpalas su alaviju ir kontaktinių lęšių tirpalas su boro rūgštimi veikia panašiai, kaip ir poliheksametilenbiguanido 0,0002 proc. tirpalas su alaviju, tačiau visos bakterijos buvo sunaikintos po 6-8 val. poveikio. Stipriausią antimi­krobinį poveikį turėjo kontaktinių lęšių tirpalas su 3 proc. vandenilio peroksido, kuris per 30 min. – 1 val. sunaikino visas tirtas bakterijas. Išvados. Akių infekcijos galima išvengti naudojant kontaktinių lęšių poliheksametilenbiguanido 0,0002 proc. tirpalą su alaviju ar kontaktinių lęšių tirpalus, kurių sudėtyje yra 3 proc. vandenilio peroksido. Lę­šius tirpaluose reikia laikyti 2 – 4 val.

scholarly journals Antibacterial activity in secondary metabolite extracts of heterotrophic bacteria against Vibrio alginolyticus, Aeromonas hydrophila, and Pseudomonas aeruginosa

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 1491
Author(s):  
Jarod Setiaji ◽  
Feli Feliatra ◽  
Hilwan Yuda Teruna ◽  
Iesje Lukistyowati ◽  
Indra Suharman ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Secondary Metabolite ◽  
Aeromonas Hydrophila ◽  
Pathogenic Bacteria ◽  
Heterotrophic Bacteria ◽  
Heterotrophic Bacterium ◽  
Vibrio Alginolyticus ◽  
Diffusion Method

Background: Disease causing bacteria such as Vibrio alginolyticus, Aeromonas hydrophila, and Pseudomonas aeruginosa present a problem for fish farming. Treatment to remove them are generally carried out using antibiotics which have side effects on fish, the environment and humans. However, the use of antibacterial compounds derived from heterotrophic bacteria serve as a good alternative for antibiotics. Therefore, this study aimed to explore antibacterial activity in the secondary metabolite extracts of heterotrophic bacteria against Vibrio alginolyticus, Aeromonas hydrophila, and Pseudomonas aeruginosa. Methods: Heterotrophic bacteria namely Bacillus sp. JS04 MT102913.1, Bacillus toyonensis JS08 MT102920.1, Bacillus cereus JS10 MT102922.1, Bacillus sp. JS11 MT102923.1, Pseudoalteromonas sp. JS19 MT102924.1, Bacillus cereus JS22 MT102926.1, and Bacillus sp. strain JS25 MT102927.1 were used in this study. The sequences of these bacteria have been deposited and are available from NCBI GenBank. Each heterotrophic bacterium was cultured on 6L nutrient broth for 8 days, and extracts produced using ethyl acetate to obtain their secondary metabolites. These extracts were tested for their phytochemical contents using FT-IR and also tested for their inhibitory property in pathogenic bacteria by agar diffusion method. Results: Phytochemical test results showed that the seven heterotrophic bacterial isolates produced terpenoid compounds. Based on the inhibitory test, the secondary metabolite extracts from Bacillus sp strain JS04 had the highest inhibitory effect on the growth of pathogenic bacteria namely, V. alginolyticus (17.5 mm), A. hydrophila (16.8 mm), and P. aeruginosa (17.3 mm). Conclusion: It was concluded that the secondary metabolite extracts of heterotrophic bacteria inhibit the growth of V. alginolyticus, A. hydrophila, and P. aeruginosa.

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