Quantitative Expression of Toll-Like Receptors TLR-7 and TLR-9 on Peripheral Blood Mononuclear Cells in Leukemias

2016 ◽  
Vol 5 (1) ◽  
pp. 17-24
Author(s):  
Mona G. Morsi ◽  
Maha M. El Gharabawy ◽  
Nahla A. Hamed ◽  
Mohamed M. El Sawy ◽  
Noha M. Abou Seada ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Toll Like Receptors ◽  
Peripheral Blood Mononuclear ◽  
Quantitative Expression ◽  
Blood Mononuclear Cells

scholarly journals Low expression of Toll-like receptors in peripheral blood mononuclear cells of pediatric patients with acute lymphoblastic leukemia

2016 ◽  
Vol 49 (2) ◽  
pp. 675-681 ◽  
Author(s):  
María Sánchez-Cuaxospa ◽  
Alejandra Contreras-Ramos ◽  
Erandi Pérez-Figueroa ◽  
Aurora Medina-Sansón ◽  
Elva Jiménez-Hernández ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Pediatric Patients ◽  
Mononuclear Cells ◽  
Lymphoblastic Leukemia ◽  
Toll Like Receptors ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Low Expression

scholarly journals Modulatory effect of Vibrio cholerae toxin co-regulated pilus on mucins, toll-like receptors and NOD genes expression in co-culture model of Caco-2 and Peripheral Blood Mononuclear Cells (PBMC)

2020 ◽  
Author(s):  
Maryam Ghasemi ◽  
Bita Bakhshi ◽  
Reza Khashei ◽  
Sara Soudi
Keyword(s):  
Vibrio Cholerae ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Intestinal Lumen ◽  
Toll Like Receptors ◽  
Peripheral Blood Mononuclear ◽  
Culture Model ◽  
Blood Mononuclear Cells

Abstract Background: Vibrio cholerae, the causative agent of cholera, as a Gram-negative pathogen tend to colonize the small intestine. The intestinal mucus layer forms mucin physical barrier, consisted from high molecular weight proteins. Regarding the role of toxin–coregulated pilus (TCP) as one of the most important colonization factors of V. cholerae, this experimental study was designed to determine the role of TcpA in induction of mucin production and its regulatory effect on innate immunity molecules including TLRs and NODs using Caco2- PBMC cocultures as an interactive model.Materials and methods: The rTcp protein was expressed in pET-28a-tcpA construct and purified using Ni-column chromatography. The identity of rTcp was confirmed by western immunoblotting analysis using anti-poly-histidine antibody. Nontoxic doses of rTcpA was determined on Caco-2 cell lines. The effects of different concentrations of rTcpA (1, 5, 10 and 50 µg/mL) on the expression of mucin 1,3, 4, toll-like receptors (TLR1, 4), and Nucleotide-binding oligomerization domain-containing proteins (NOD1, 2) genes were evaluated in a co-culture model of human colon carcinoma cell line (Caco-2) and Peripheral Blood Mononuclear cells (PBMCs).Results:The rTcpA protein of V. choleraewas expressed in BL21 E. coli and confirmed by western blotting. The rTcpA showed a statistically significant effect on the expression of muc genes (MUC3 and MUC4) in a dose-dependent manner.This finding is supposed to facilitate physical adhesion and colonization of V. cholerae in intestinal lumen. The rTcpA moderately stimulated the expression of tlr4 and overexpressed tlr1, both of which are supposed to induce a mucosal protective response against bacterial infection and would help a promising protection in prophylaxis applications. No change in NOD2 expression might be attributed to the non-invasive nature of V. cholerae as an intestinal pathogen.Conclusion: In conclusion, the rTcpA protein of V. cholerae showed a statistically significant modulatory effect on the human gut epithelium gene expression which would help promising protection in prophylaxis applications.

Sign in / Sign up

Export Citation Format

Share Document