scholarly journals Steroid Hormone Formation IN VITRO by Developing Corpora Lutea of the Rabbit Ovary

1977 ◽  
Vol 53 (8) ◽  
pp. 970-981
Author(s):  
Akira SUZUKI ◽  
Takahide MORI ◽  
Yasuhiko FUJITA ◽  
Toshio NISHIMURA
Keyword(s):  
Steroid Hormone ◽  
Corpora Lutea ◽  
Hormone Formation

STEROID HORMONE FORMATION BY THE RAT OVARY.

1966 ◽  
Vol 51 (1) ◽  
pp. 131-139 ◽  
Author(s):  
Bernard F. Rice ◽  
Albert Segaloff
Keyword(s):  
Steroid Hormone ◽  
Ovarian Tissue ◽  
Female Rats ◽  
Male Rats ◽  
Corpora Lutea ◽  
Castrate Male ◽  
Rat Ovary ◽  
17 Hydroxyprogesterone ◽  
Hormone Formation

ABSTRACT Ovaries were transplanted to the spleens of castrate male rats. After 120 days, slices of ovarian tissue, composed predominantly of corpora lutea, were incubated in Krebs-Ringer bicarbonate medium containing 50 μc acetate-1-14C. Radioactive steroid formation was assessed quantitatively by reverse isotope dilution. The formation of radioactive progesterone and 20α-hydroxy-pregn-4-en-3-one was established. The formation of radioactive 3β-hydroxy-pregn-5-en-20-one, androst-4-ene-3,17-dione, 17-hydroxyprogesterone, testosterone, oestrone and 17β-oestradiol could not be established. It appears that the corpus luteum of the rat, induced by endogenous gonadotrophins, forms only progestins from acetate-1-14C. Contrary to results previously obtained with ovarian tissue transplanted to female rats, radioactive steroid formation in vitro appeared to be augmented by luteinizing hormone (NIH-LH-S1) added to the incubation flasks. Administration of human chorionic gonadotrophin (200 IU/day) for 5 days prior to autopsy did not enhance acetate-1-14C incorporation in vitro.

Regulation of steroid hormone synthesis by human corpora lutea: failure of follicle-stimulating hormone to support steroidogenesis in vivo and in vitro

1989 ◽  
Vol 51 (4) ◽  
pp. 628-633 ◽  
Author(s):  
Luigi. Devoto ◽  
Margarita. Vega ◽  
Veronica. Navarro ◽  
Teresa. Sir ◽  
Francisco. Alba ◽  
...  
Keyword(s):  
Steroid Hormone ◽  
Follicle Stimulating Hormone ◽  
Corpora Lutea ◽  
Hormone Synthesis ◽  
Stimulating Hormone

Comparison of ovarian oestradiol and progesterone secretion, in vitro, between ewe lambs and ewes

2005 ◽  
Vol 2005 ◽  
pp. 149-149
Author(s):  
M. A. Younes ◽  
N. F. G. Beck ◽  
M. T. Rose ◽  
B. Davies
Keyword(s):  
Reproductive Performance ◽  
Steroid Hormone ◽  
Corpora Lutea ◽  
Ovarian Hormone ◽  
Hormone Production ◽  
Previous Evidence ◽  
Ewe Lambs ◽  
Progesterone Secretion ◽  
Ewe Lamb

Reproductive performance of ewe lambs is lower than that of adult ewes (Quirke 1979). This is mainly the result of preimplantation losses, which can exceed 50% of fertilised eggs. Previous evidence from this laboratory suggests that these losses may be associated with abnormal ovarian hormone production (Davies and Beck 1993). Khan (1999) demonstrated that blood progesterone levels during the oestrous cycle and pregnancy were lower in ewe lambs than in ewes. Furthermore, both progesterone and oestradiol concentrations were lower in ewe lambs, than in ewes, following gonadotrophin stimulation (Khan, Beck and Khalid 1999). These results suggest that ewe lamb corpora lutea and follicles secrete less progesterone and oestradiol, respectively, than those of ewes. The aim of this study was to determine whether there is a difference in vitro steroid hormone production by corpora lutea and follicles, from ewe lambs and ewes.

scholarly journals Steroid Hormone Formation In Vitro by Human Ovarian Follicles During the Process of Maturation and Ovulation Correlated with Follicle Structures

1978 ◽  
Vol 54 (9) ◽  
pp. 1043-1057
Author(s):  
Yasuhiko FUJITA ◽  
Takahide MORI ◽  
Akira SUZUKI ◽  
Yasushi KINOSHITA ◽  
Toshio NISHIMURA
Keyword(s):  
Steroid Hormone ◽  
Ovarian Follicles ◽  
Human Ovarian Follicles ◽  
Hormone Formation

STEROID HORMONE METABOLISM IN RESPONSIVE TISSUES IN VITRO

1971 ◽  
Vol 68 (1_Suppl) ◽  
pp. S279-S294 ◽  
Author(s):  
Paul Robel
Keyword(s):  
Steroid Hormone ◽  
Physiological Activity ◽  
Physiological State ◽  
Target Cells ◽  
Target Tissue ◽  
Hormone Metabolism ◽  
Metabolizing Enzymes ◽  
Relationship Of

ABSTRACT Of the information available on steroid hormone metabolism in responsive tissues, only that relating hormone metabolism to physiological activity is reviewed, i. e. metabolite activity in isolated in vitro systems, binding of metabolites to target tissue receptors, specific steroid hormone metabolizing enzymes and relationship of hormone metabolism to target organ physiological state. Further, evidence is presented in the androgen field, demonstrating 5α-reduced metabolites, formed inside the target cells, as active compounds. This has led to a consideration of testosterone as a »prehormone«. The possibility that similar events take place in tissues responding to progesterone is discussed. Finally, the role of hormone metabolism in the regulation of hormone availability and/or renewal in target cells is discussed. In this context, reference is made to the potential role of plasma binding proteins and cytosol receptors.

Blended Effects of Herbal Components of Tokishakuyakusan on Somatomedin C/Insulin-like Growth Factor 1 Level in Rat Corpus Luteum

1991 ◽  
Vol 19 (01) ◽  
pp. 61-64 ◽  
Author(s):  
Satoshi Usuki
Keyword(s):  
Growth Factor ◽  
Corpus Luteum ◽  
Corpora Lutea ◽  
Insulin Like Growth Factor ◽  
Somatomedin C ◽  
Factor I ◽  
Peony Root ◽  
Growth Factor I ◽  
Herbal Components

The effect of herbal components of Tokishakuyakusan on somatomedin C/insulin-like growth factor I (IGF-1) level in medium from rat corpora lutea incubated in vitro was examined. Hoelen + peony root + Japanese angelica root, hoelen + peony root, hoelen + Japanese angelica root or peony root + Japanese angelica root decreased the IGF-1 level. The data suggest that constituent herbal components of Tokishakuyakusan regulate the IGF-1 level by rat corpora lutea.

STIMULATION BY HUMAN CHORIONIC GONADOTROPHIN OF OESTRADIOL PRODUCTION BY DISPERSED CELLS FROM HUMAN CORPUS LUTEUM: COMPARISON WITH PROGESTERONE PRODUCTION; UTILIZATION OF EXOGENOUS TESTOSTERONE

1981 ◽  
Vol 91 (2) ◽  
pp. 197-203 ◽  
Author(s):  
M. C. RICHARDSON ◽  
G. M. MASSON
Keyword(s):  
Luteal Phase ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Cell Suspensions ◽  
Corpora Lutea ◽  
Progesterone Production ◽  
Late Luteal Phase ◽  
Oestradiol Production ◽  
Dispersed Cells

Cell suspensions were prepared from tissue samples of human corpora lutea obtained during the mid- and late-luteal phase of the menstrual cycle. Both oestradiol and progesterone production by dispersed cells were stimulated by similar concentrations of human chorionic gonadotrophin (hCG). As the degree of stimulation of production by hCG was greater for progesterone than for oestradiol (five- to tenfold compared with two- to threefold higher than basal production), the ratio of progesterone to oestradiol produced varied according to the level of trophic stimulation. A comparison of cell suspensions prepared from mid- and late-luteal phase corpora lutea, exposed to the same concentration of hCG (10 i.u./ml) in vitro, did not reveal a shift to oestradiol production in the late-luteal phase. Provision of additional testosterone during incubation raised the level of oestradiol production by dispersed luteal cells. At an optimum concentration of testosterone (1 μmol/l), oestradiol synthesis was not raised further in the presence of hCG or N6, O2-dibutyryl cyclic AMP, suggesting a lack of induction or activation of the aromatase system by gonadotrophin in short-term cultures. Basal and stimulated levels of progesterone production were not significantly impaired in the presence of testosterone.

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