Determination of the Normal Range of Serum LH and FSH Levels in Normal Adult Males

SUMMARY The incorporation of [35S]methionine into protein in various regions of the brain and in the anterior pituitary, and serum luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels were measured at 6 h intervals throughout a 24 h period in the following groups of Wistar rats: (1) normal adult males and females; (2) adult genetic males or females which had been respectively 'feminized' or 'masculinized' by androgen deprivation or administration in neonatal life. Similar measurements were made at 12 h intervals in adult male rats which had been castrated at 7 or 15 days of age. Serum LH levels showed a circadian rhythmicity in normal adult animals of both sexes, with peak levels in the male occurring 6 h earlier than those in the female. There was no statistically significant circadian rhythm in FSH levels in any group of animals. In all groups of castrated animals LH and FSH levels were raised but no circadian rhythms were observed. Incorporation of [35S]methionine into protein in all cerebral areas showed circadian rhythms, the peak values of which, in the adult males, were almost 8 h (120°) out of phase with those of the adult females. In the 'feminized' genetic males or 'masculinized' genetic females the rhythmic phase was reversed to that of the opposite genetic sex. Animals castrated at 7 days or 15 days of age did not appear to show a rhythm. A 12 h rhythm of incorporation was apparent in the anterior pituitary of the normal adult male and the adult 'masculinized' female; no significant rhythm was seen in the normal adult female and the 'feminized' male. It is suggested that a 'female type' rhythm of incorporation in the brain may be associated with the maintenance of oestrous cycles.

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Significant Population Variation in Adult Male Height Associated with the Y Chromosome and the Aromatase Gene

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jcem.86.9.7875 ◽

2001 ◽

Vol 86 (9) ◽

pp. 4147-4150 ◽

Cited By ~ 23

Author(s):

Justine A. Ellis ◽

Margaret Stebbing ◽

Stephen B. Harrap

Keyword(s):

Confidence Interval ◽

Y Chromosome ◽

Adult Height ◽

Large Population ◽

Population Sample ◽

Population Variation ◽

Normal Adult ◽

Adult Males ◽

Gene Encoding

The determination of human adult height is dependent on both environmental and genetic factors. Rare causes of abnormal stature have been identified, including mutations in the gene encoding aromatase (CYP19) and regions on the Y chromosome. However, the possible role of these loci in the genetic control of normal adult height is unknown. We have performed an association study using common biallelic polymorphisms within CYP19 and the Y chromosome to determine whether these loci are associated with variation in height in 413 adult males and 335 females drawn at random from a large population sample. An association between CYP19 and height was found (difference, 2.0 cm; 95% confidence interval, 0.16–3.8; P = 0.003), but this was more evident in men (difference, 2.3 cm; 95% confidence interval, 0.38–4.4; P = 0.05) than women (difference, 0.2 cm; 95% confidence interval, −2.1 to 1.6; P = 0.94). An association was also found with the Y chromosome (P = 0.009; difference of 1.9 cm; 95% confidence interval, 0.5–3.4). Additionally, when men were grouped according to haplotypes of the CYP19 and Y chromosome polymorphisms, a difference of 4.2 cm (95% confidence interval, 0.67–7.3) was detected (P = 0.004). These results suggest that in men, genetic variation in CYP19 and on the Y chromosome are involved in determining normal adult height, and that these loci may interact in an additive fashion.

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Fluorimetric Determination of Creatine

Clinical Chemistry ◽

10.1093/clinchem/6.6.537 ◽

1960 ◽

Vol 6 (6) ◽

pp. 537-548 ◽

Cited By ~ 41

Author(s):

Rex B Conn

Keyword(s):

Alkaline Solution ◽

Normal Adult ◽

Adult Males ◽

Fluorimetric Determination ◽

Fluorimetric Method ◽

Fluorescent Products ◽

Colorimetric Methods

Abstract A new fluorimetric method for determining creatine in serum, blood, and urine is described. The method is based upon the coupling of guanidine compounds with ninhydrin in alkaline solution to produce highly fluorescent products. In degree of sensitivity and simplicity the method possesses several advantages over currently used colorimetric methods, and creatine can be determined in concentrations as low as 1.0 X 10-7M. The serum creatine concentration was found to be 0.41 mg.% in normal adult males, who excrete only small amounts of creatine in the urine.

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Characterization of Tamm-Horsfall Urinary Glycoprotein

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010007254x ◽

1973 ◽

Vol 31 ◽

pp. 420-421

Author(s):

John P. Robinson ◽

J. David Puett

Keyword(s):

Electron Microscopy ◽

Circular Dichroism ◽

Secondary Structure ◽

Quaternary Structure ◽

Normal Adult ◽

Morphological Properties ◽

Adult Males ◽

Circular Dichroïsm ◽

Urinary Glycoprotein

Much work has been reported on the chemical, physical and morphological properties of urinary Tamm-Horsfall glycoprotein (THG). Although it was once reported that cystic fibrotic (CF) individuals had a defective THG, more recent data indicate that THG and CF-THG are similar if not identical.No studies on the conformational aspects have been reported on this glycoprotein using circular dichroism (CD). We examined the secondary structure of THG and derivatives under various conditions and have correlated these results with quaternary structure using electron microscopy.THG was prepared from normal adult males and CF-THG from a 16-year old CF female by the method of Tamm and Horsfall. CF female by the method of Tamm and Horsfall.

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Profil Penyakit Malaria Di Rumah Sakit Islam Siti Rahmah Padang Tahun 2018

Health & Medical Journal ◽

10.33854/heme.v2i2.546 ◽

2020 ◽

Vol 2 (2) ◽

pp. 08-15

Author(s):

Rahma Triyana ◽

Salmi Salmi

Keyword(s):

Observational Study ◽

Frequency Distribution ◽

Cross Section ◽

Laboratory Tests ◽

Age Group ◽

Normal Range ◽

Cross Sectional ◽

West Sumatra ◽

Severity Of The Disease

Malaria is one of the health problems in Indonesia, especially West Sumatra. Determination of the description of Malaria disease in an area is needed to determine the spread and severity of the disease. This study aims to determine the frequency distribution according to age, sex and place of residence, description of the types of Plasmodium causes of Malaria and hematological features in Malaria patients at Siti Rahmah Padang Hospital in 2018. This type of research is a descriptive observational study with an approach or design cross section (cross sectional). The frequency distribution of Malaria sufferers in Siti Rahmah Padang Hospital in 2018 according to the highest age was in the age group 21-30 years as many as 28 cases (36.8%), the highest sex among men was 46 (60.5%) and the highest number of residences was found in Koto Tangah sub-district there were 31 cases (40.8%). The type of Plasmodium found in Malaria cases in Siti Rahmah Padang Hospital in 2018 was P. vivax (73 cases (96.05%)) and P. falciparum (3 cases (3.95%)). The results of laboratory tests on Hb, hematocrit, platelet and leukocyte levels in Malaria positive patients in Siti Rahmah Padang Hospital in 2018 were in the normal range.

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Profil Penyakit Malaria di Rumah Sakit Islam Siti Rahmah Padang Tahun 2018

Health & Medical Journal ◽

10.33854/heme.v2i2.452 ◽

2020 ◽

Vol 2 (2) ◽

pp. 08-15

Author(s):

Rahma Triyana Y ◽

Salmi Salmi

Keyword(s):

Observational Study ◽

Frequency Distribution ◽

Cross Section ◽

Laboratory Tests ◽

Age Group ◽

Normal Range ◽

Cross Sectional ◽

West Sumatra ◽

Severity Of The Disease

Malaria is one of the health problems in Indonesia, especially West Sumatra. Determination of the description of Malaria disease in an area is needed to determine the spread and severity of the disease. This study aims to determine the frequency distribution according to age, sex and place of residence, description of the types of Plasmodium causes of Malaria and hematological features in Malaria patients at Siti Rahmah Padang Hospital in 2018. This type of research is a descriptive observational study with an approach or design cross section (cross sectional). The frequency distribution of Malaria sufferers in Siti Rahmah Padang Hospital in 2018 according to the highest age was in the age group 21-30 years as many as 28 cases (36.8%), the highest sex among men was 46 (60.5%) and the highest number of residences was found in Koto Tangah sub-district there were 31 cases (40.8%). The type of Plasmodium found in Malaria cases in Siti Rahmah Padang Hospital in 2018 was P. vivax (73 cases (96.05%)) and P. falciparum (3 cases (3.95%)). The results of laboratory tests on Hb, hematocrit, platelet and leukocyte levels in Malaria positive patients in Siti Rahmah Padang Hospital in 2018 were in the normal range.

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HAPTEN-RADIOIMMUNOASSAY OF PLASMA OESTRADIOL

Acta Endocrinologica ◽

10.1530/acta.0.0720330 ◽

1973 ◽

Vol 72 (2) ◽

pp. 330-344 ◽

Cited By ~ 10

Author(s):

Peter Doerr

Keyword(s):

Adult Male ◽

Normal Values ◽

Normal Adult ◽

Adult Males ◽

Reagent Blank ◽

Keyhole Limpet Haemocyanin ◽

Ether Extraction ◽

The Mean ◽

Assay Precision ◽

Plasma Oestradiol

ABSTRACT A hapten-radioimmunoassay for plasma oestradiol is described and information about the reliability of the method is given in detail. Oestradiol-3-hemisuccinate coupled to keyhole limpet haemocyanin is used for immunization of rabbits. The antiserum utilized for the assay is characterized by its titer, affinity and specificity. Following ether extraction and NaOH-light petroleum partition oestradiol is separated from crossreacting oestrogens by TLC. Oxidation of oestradiol on the plate is prevented by mercaptoethanol. To separate free and antibody bound ligand 250 μg dextran-coated charcoal per tube is used in the presence of bovine serum gammaglobulin (1 mg/ml). The between-assay precision based on 15 different determinations of control samples from normal adult male plasma was 9.4% (C. V.). The mean reagent blank value of 31 determinations was equivalent to 0.3 pg oestradiol and the detection limit in terms of the 99% confidence limit for a single blank value, was equivalent to 4.3 pg oestradiol. A procedure for detecting plasma blanks is described. Plasma oestradiol is separated from approximately all concomitant substances originally present in the sample by enzymatic conversion into oestrone and a second TLC. No plasma blanks could be detected with respect to normal adult male plasma. Normal values for adult males based on 51 subjects were characterized by a median of 17.2 pg/ml and the 95 percentiles of 9.5–27.6.

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FEEDBACK CONTROL OF FSH IN THE MALE: ROLE OF OESTROGEN

Acta Endocrinologica ◽

10.1530/acta.0.0740449 ◽

1973 ◽

Vol 74 (3) ◽

pp. 449-460 ◽

Cited By ~ 11

Author(s):

Patrick C. Walsh ◽

Ronald S. Swerdloff ◽

William D. Odell

Keyword(s):

Luteinizing Hormone ◽

Follicle Stimulating Hormone ◽

Oestrogen Therapy ◽

Serum Concentrations ◽

Elderly Men ◽

Normal Range ◽

Oestrogen Treatment ◽

Serum Lh ◽

Higher Doses

ABSTRACT Serum follicle stimulating hormone (FSH) and luteinizing hormone (LH) were measured by radioimmunoassay in a group of elderly men following castration and oestrogen therapy. Prior to orchiectomy, mean serum concentrations of LH and FSH were within the normal range. Two days following castration, serum LH concentrations increased in all eight patients; higher levels of LH were subsequently measured in all but one patient after periods of time ranging from 49 to 210 days. Serum FSH levels, measured in three patients following castration, increased in a pattern parallel to LH changes. Ethinyl oestradiol (EOe) in doses ranging from 5 to 300 μg/day was administered to ten men who had been castrated 3 to 72 months earlier. Oestrogen treatment suppressed both LH and FSH in a parellel manner in nine of ten patients. LH was first suppressed to intact levels in one of eight patients treated with 20 μg/day of EOe, in two of six patients treated with 50 μg/day, and in one patient by 80 μg/day. FSH was not suppressed to precastration levels until 50 μg/day of EOe was administered; this dose suppressed three of six patients. Higher doses of EOe (150–300 μg/day) suppressed both LH and FSH to levels below the sensitivity of the assay. These data fail to demonstrate any differential effect of oestrogen on LH and FSH release.

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