Molecular diagnosis of toxoplasmosis: evaluation of automated DNA extraction using eMAG® (bioMérieux) on buffy coat, cerebrospinal and bronchoalveolar lavage fluids

2020 ◽  
Vol 58 (3) ◽  
pp. e91-e93
Author(s):  
Fanny Zhao ◽  
Lionnel Bulin ◽  
Hélène Fricker-Hidalgo ◽  
Quentin Renaut ◽  
Marie Gladys Robert ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Dna Extraction ◽  
Molecular Diagnosis ◽  
Buffy Coat ◽  
Automated Dna Extraction

Automated DNA extraction from frozen blood using the abbott M1000™ for HLA-DRB sequencing-based typing

2003 ◽  
Vol 64 (10) ◽  
pp. S95 ◽  
Author(s):  
Jeff T. Silva ◽  
Christine Y. Wong ◽  
JoAnn L. Dileanis ◽  
Chad M. Dunn ◽  
Chaka C. Impraim
Keyword(s):  
Dna Extraction ◽  
Automated Dna Extraction

scholarly journals Improved DNA Extraction Method for Molecular Diagnosis from Smaller numbers of Cells

2014 ◽  
Vol 46 (3) ◽  
pp. 99-105 ◽  
Author(s):  
Seo Young Oh ◽  
Jeong Yeon Han ◽  
So Ra Lee ◽  
Hoon Taek Lee
Keyword(s):  
Dna Extraction ◽  
Molecular Diagnosis ◽  
Extraction Method ◽  
Dna Extraction Method

scholarly journals Automated DNA extraction platforms offer solutions to challenges of assessing microbial biofouling in oil production facilities

AMB Express ◽  
2012 ◽  
Vol 2 (1) ◽  
pp. 60 ◽  
Author(s):  
Athenia L Oldham ◽  
Heather S Drilling ◽  
Blake W Stamps ◽  
Bradley S Stevenson ◽  
Kathleen E Duncan
Keyword(s):  
Dna Extraction ◽  
Oil Production ◽  
Automated Dna Extraction ◽  
Production Facilities

Automated DNA extraction of single dog hairs without roots for mitochondrial DNA analysis

2012 ◽  
Vol 6 (2) ◽  
pp. 277-281 ◽  
Author(s):  
Bram Bekaert ◽  
Maarten H.D. Larmuseau ◽  
Maarten P.M. Vanhove ◽  
Anouschka Opdekamp ◽  
Ronny Decorte
Keyword(s):  
Mitochondrial Dna ◽  
Dna Extraction ◽  
Dna Analysis ◽  
Mitochondrial Dna Analysis ◽  
Automated Dna Extraction

Molecular diagnosis of toxoplasmosis: value of the buffy coat for the detection of circulating Toxoplasma gondii

2015 ◽  
Vol 82 (4) ◽  
pp. 289-291 ◽  
Author(s):  
Marie-Pierre Brenier-Pinchart ◽  
Elodie Capderou ◽  
Rose-Laurence Bertini ◽  
Sébastien Bailly ◽  
Hélène Fricker-Hidalgo ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Diagnosis ◽  
Buffy Coat

scholarly journals Erratum to: Evaluation of DNA extraction kits for molecular diagnosis of human Blastocystis subtypes from fecal samples

2011 ◽  
Vol 110 (2) ◽  
pp. 1063-1063 ◽  
Author(s):  
Hisao Yoshikawa ◽  
Funda Dogruman-Al ◽  
Songul Turk ◽  
Semra Kustimur ◽  
Neriman Balaban ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Molecular Diagnosis ◽  
Fecal Samples

scholarly journals Optimisation of an Automated DNA Extraction Method for Bone and Teeth Samples and Applicability to Two Forensic Cases

2021 ◽  
Vol 1 (3) ◽  
pp. 194-201
Author(s):  
Jasmin Zoranjic ◽  
Jasmine W. Tay ◽  
Nicholas S. Mountford ◽  
Marie S. Rye
Keyword(s):  
Dna Extraction ◽  
Extraction Method ◽  
Disaster Victim Identification ◽  
Victim Identification ◽  
Dna Quality ◽  
Dna Extraction Method ◽  
Organic Extraction ◽  
Automated Dna Extraction ◽  
Dna Profiles ◽  
Processing Steps

Bones and teeth are highly challenging sources of DNA in forensic science and human remains identification, requiring multiple laborious processing steps. In this study, we compared an organic phenol–chloroform method to the QIAamp® DNA Investigator and PrepFiler Express BTA™ methods in order to identify the most efficient automated DNA extraction method for bones and teeth. Results from individual tooth powder replicates showed that the PrepFiler Express BTA™ method extracted the highest yields of DNA per mg of tooth powder, returning a minimum of 20/21 PowerPlex® 21 loci. Samples extracted using the organic extraction or QIAamp® DNA Investigator methods produced PowerPlex® 21 profiles displaying a ski-slope morphology. The improved DNA quality and yield from the PrepFiler Express BTA™ method was verified using aged samples, where higher DNA yields per mg of powder and more informative profiles were obtained. Furthermore, the PrepFiler Express BTA™ method subsequently provided useful DNA profiles for two forensic cases involving degraded bone samples. Overall, this study showed that the PrepFiler Express BTA™ chemistry is a reliable and robust method for DNA extraction from bone and teeth samples, and will allow larger numbers of samples to be efficiently extracted in the event of a Disaster Victim Identification event.

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