Effect of silver nanoparticles on human primary keratinocytes

Abstract Silver nanoparticles (AgNPs) have many biological applications in biomedicine, biotechnology and other life sciences. Depending on the size, shape and the type of carrier, AgNPs demonstrate different physical and chemical properties. AgNPs have strong antimicrobial, antiviral and antifungal activity, thus they are used extensively in a range of medical settings, particularly in wound dressings but also in cosmetics. This study was undertaken to examine the potential toxic effects of 15 nm polyvinylpyrrolidone-coated AgNPs on primary normal human epidermal keratinocytes (NHEK). Cells were treated with different concentrations of AgNPs and then cell viability, metabolic activity and other biological and biochemical aspects of keratinocytes functioning were studied. We observed that AgNPs decrease keratinocyte viability, metabolism and also proliferatory and migratory potential of these cells. Moreover, longer exposure resulted in activation of caspase 3/7 and DNA damage. Our studies show for the first time, that AgNPs may present possible danger for primary keratinocytes, concerning activation of genotoxic and cytotoxic processes depending on the concentration.

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Effects of silver nanoparticles on human dermal fibroblasts and epidermal keratinocytes

Human & Experimental Toxicology ◽

10.1177/0960327115611969 ◽

2016 ◽

Vol 35 (9) ◽

pp. 946-957 ◽

Cited By ~ 26

Author(s):

A Galandáková ◽

J Franková ◽

N Ambrožová ◽

K Habartová ◽

V Pivodová ◽

...

Keyword(s):

Wound Healing ◽

Silver Nanoparticles ◽

Biomedical Application ◽

Dermal Fibroblasts ◽

Epidermal Keratinocytes ◽

Topical Agent ◽

Human Dermal Fibroblasts ◽

Human Epidermal Keratinocytes ◽

Normal Human ◽

Treatment Of Wounds

Biomedical application of silver nanoparticles (AgNPs) has been rapidly increasing. Owing to their strong antimicrobial activity, AgNPs are used in dermatology in the treatment of wounds and burns. However, recent evidence for their cytotoxicity gives rise to safety concerns. This study was undertaken as a part of an ongoing programme in our laboratory to develop a topical agent for wound healing. Here, we investigated the potential toxicity of AgNPs using normal human dermal fibroblasts (NHDF) and normal human epidermal keratinocytes (NHEK) with the aim of comparing the effects of AgNPs and ionic silver (Ag-I). Besides the effect of AgNPs and Ag-I on cell viability, the inflammatory response and DNA damage in AgNPs and Ag-I–treated cells were examined. The results showed that Ag-I were significantly more toxic than AgNPs both on NHDF and NHEK. Non-cytotoxic concentrations of AgNPs and Ag-I did not induce DNA strand breaks and did not affect inflammatory markers, except for a transient increase in interleukin 6 levels in Ag-I–treated NHDF. The results showed that AgNPs are more suitable for the intended application as a topical agent for wound healing up to the concentration 25 µg/mL.

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The effects of cross-linked/uncross-linked electrospun fibrinogen/polycaprolactone nanofibers on the proliferation of normal human epidermal keratinocytes

Journal of Polymer Engineering ◽

10.1515/polyeng-2017-0350 ◽

2018 ◽

Vol 38 (10) ◽

pp. 945-953

Author(s):

Mohamad Javad Mirzaei-Parsa ◽

Hossein Ghanbari ◽

Naghmeh Bahrami ◽

Shahryar Hadadi-Abianeh ◽

Reza Faridi-Majidi

Keyword(s):

Tissue Engineering ◽

Loss Rate ◽

Chemical Properties ◽

Epidermal Keratinocytes ◽

Cross Linking ◽

Human Epidermal Keratinocytes ◽

Normal Human Epidermal Keratinocytes ◽

Nanofibrous Scaffolds ◽

Normal Human ◽

Diphenyl Tetrazolium Bromide

AbstractThe aim of this study was an investigation on the proliferation rate of normal human epidermal keratinocytes (NHEK) on the cross-linked and uncross-linked fibrinogen/polycaprolactone (Fbg/PCL) nanofibers to determine a suitable scaffold for skin tissue engineering. Nanofibrous scaffolds were prepared by electrospinning of different weight ratios of Fbg to PCL and were analyzed as morphology, surface chemical properties and cytocompatibility by scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, respectively. The diameters of the blended uncross-linked scaffolds were in the range of 124±43 nm–209±155 nm. Cross-linking of scaffolds with glutaraldehyde did not make a significant change in the diameter of blended scaffolds in 16 h. Cross-linking also improved the tensile strength and weight loss rate of scaffolds. However, cross-linking demonstrated an unfavorable effect on the attachment and proliferation of NHEK cells. The proliferation study revealed that uncross-linked scaffolds containing 50% and 70% Fbg provide a better environment for the growth of NHEK cells, and can be considered promising scaffolds in tissue engineering applications.

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