scholarly journals Evaluation of the phenolic compounds and the antioxidant potentials of Vitex agnus-castus L. leaves and fruits

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Ceren Kımna ◽  
Tuğçe Fafal
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Leaf Extract ◽  
Phenolic Content ◽  
Leaf Extracts ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Antioxidant Agents ◽  
Agnus Castus ◽  
Chaste Tree

Abstract Objectives In this study, we aim at deciphering the phenolic content of Vitex agnus-castus L. leaf and fruit extracts prepared with different methods and relate it to their antioxidant activity. Methods In this study, phenolic compounds and the antioxidant potential of the ethanol fruit and leaf extracts of V. agnus-castus L. (Chaste tree) were evaluated spectrometrically. Furthermore, selected polyphenols, i.e., chlorogenic acid and rutin, were determined by the HPLC-DAD method qualitatively and quantitatively. Results The results obtained from leaf and fruit extracts were compared with a commercial product (CP) containing the fruit extract of V. agnus-castus. Leaf extract was found to be richer in flavonoids when compared to the fruit counterparts. Accordingly, they also showed higher antioxidant activity. Conclusions Extracts prepared here can be considered as promising antioxidant agents for future therapeutic formulations.

scholarly journals Phenolic Content and Antioxidant Activity in Trifolium Germplasm from Different Environments

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 298 ◽  
Author(s):  
Aldo Tava ◽  
Łukasz Pecio ◽  
Roberto Lo Scalzo ◽  
Anna Stochmal ◽  
Luciano Pecetti
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Chemical Structure ◽  
Dry Matter ◽  
Phenolic Content ◽  
Total Phenolics ◽  
Adaptive Strategies ◽  
Phenolic Composition ◽  
Leaf Extracts ◽  
Plant Environment

Phenolics are important mediators in plant-environment interactions. The presence and concentration of phenolic compounds and their antioxidant activity were evaluated in leaves and flowers of a set of Trifolium species originating from contrasting environments encompassing lowland and mountain sites. The current germplasm proved a great reservoir of phenolic compounds, with different chemical structure and, possibly, diversified biological activity. Germplasm groups with specific phenolic composition were observed. In some cases, different patterns bore a taxonomic meaning. Lowland germplasm showed higher concentration of total phenolics in leaves than mountain accessions (50.30 vs. 34.19 mg/g dry matter (DM)), while the latter had higher concentration in flowers (114.16 vs. 57.44 mg/g DM). Outstanding concentration of isoflavones was observed in leaves of lowland germplasm (24.19 mg/g DM), and of both proanthocyanidins and flavonoids in flowers of mountain germplasm (53.81 and 56.62 mg/g DM, respectively). The pattern of phenolic composition in lowland and mountain germplasm was suggestive of different adaptive strategies. Three assays of antioxidant activity were tested, which were characterised by rather different reactivity towards phenolic composition. The scavenging activity was higher for leaf extracts of lowland germplasm, and for flower extracts of mountain germplasm. Besides identifying germplasm of interest, this study also suggested possible links between environmental factors and concentration and composition of phenolic compounds.

scholarly journals Antibacterial, antifungal and antioxidant activity of Dichapetalum gelonioides (Roxb.) Engl. (Dichapetalaceae)

2018 ◽  
Vol 8 (5) ◽  
pp. 263-267
Author(s):  
GS Priyanka ◽  
Nitish A Bharadwaj ◽  
MB Sachin ◽  
TR Prashith Kekuda
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
The Family ◽  
Poisoned Food Technique ◽  
Bacteria And Fungi ◽  
Dose Dependent

Objectives: Dichapetalum gelonioides (Roxb.) Engl. belongs to the family Dichapetalaceae. In the present study, we investigated antibacterial, antifungal and antioxidant activity of methanolic extract of leaf and fruit of D. gelonioides. Methods: Maceration process was carried out for extraction of leaf and fruit of D. gelonioides. Agar well diffusion method was employed to evaluate antibacterial activity of extracts against gram positive and gram negative bacteria. Poisoned food technique was performed to investigate antifungal activity of extracts against two seed-borne fungi. Antioxidant activity was evaluated by DPPH radical scavenging and ferric reducing assays.  Results: Both leaf and fruit extracts were effective in causing inhibition of all test bacteria. Highest and least inhibitory activity was observed against Bacillus cereus and Escherichia coli respectively. Both Aspergillus niger and Bipolaris sp. were inhibited to >50% by leaf and fruit extracts. Extent of inhibition of Bipolaris sp. was slightly higher when compared to A. niger. Both leaf and fruit extracts showed a dose dependent scavenging of DPPH radicals with high activity being showed by leaf extract. Leaf extract was shown to exhibit marked reducing potential than fruit extract. Conclusions: Overall, leaf extract was shown to be more effective in displaying antioxidant activity and causing inhibition of bacteria and fungi when compared to fruit extract. The results indicate that the plant possess active principles which are to be purified, characterized and subjected for antimicrobial and antioxidant assays in further studies. Keywords: Dichapetalum gelonioides, Maceration, Agar well diffusion, Poisoned food technique, DPPH, Ferric reducing

scholarly journals Comparative Study of Leaf and Rootstock Aqueous Extracts of Foeniculum vulgare on Chemical Profile and In Vitro Antioxidant and Antihyperglycemic Activities

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Karima Sayah ◽  
Nasreddine El Omari ◽  
Mourad Kharbach ◽  
Abdelhakim Bouyahya ◽  
Rabie Kamal ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Leaf Extract ◽  
Quinic Acid ◽  
Leaf Extracts ◽  
Foeniculum Vulgare ◽  
Main Compound ◽  
Phenolic Components ◽  
Antidiabetic Effects

Foeniculum vulgare is a medicinal plant used in Moroccan folk medicine to treat several diseases such as diabetes. The aim of this study was to determine the phenolic bioactive compounds and to evaluate the antioxidant and antihyperglycemic activities of Foeniculum vulgare leaf and rootstock extracts. Phenolic compounds of F. vulgare rootstock and leaf extracts were determined using HPLC-DAD-QTOFMS analysis. The antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) radicals. Moreover, the in vitro antihyperglycemic effects were tested by measuring the inhibition of α-amylase and α-glucosidase activities. HPLC-DAD-QTOFMS analysis identified thirty-two phenolic components in both leaf and rootstock extracts. Caffeic acid, quinic acid, and chlorogenic acid were the major compounds of F. vulgare leaf extract (FVLE), while the main compound of F. vulgare rootstock extracts (FVRE) was quinic acid. In the DPPH assay, F. vulgare leaf extract showed important antioxidant activity (IC50 = 12.16 ± 0.02 μg/mL) than F. vulgare rootstock extract (IC50 = 34.36 ± 0.09 μg/mL). Moreover, fennel leaf extracts revealed also the most powerful antioxidant activity (IC50 = 22.95 ± 0.4 μg/mL) in the ABTS assay. The in vitro antihyperglycemic activity showed that F. vulgare rootstock extract exhibited a remarkable inhibitory capacity (IC50 = 194.30 ± 4.8 μg/mL) of α-amylase compared with F. vulgare leaf extract (IC50 = 1026.50 ± 6.5 μg/mL). Furthermore, the inhibition of α-glucosidase was more importantly with F. vulgare rootstock (IC50 of 165.90 ± 1.2 μg/mL) than F. vulgare leaf extracts (203.80 ± 1.3 μg/mL). The funding of this study showed that F. vulgare rootstock and leaf extracts presented several phenolic compounds and showed important antioxidant and antidiabetic effects. We suggest that the identified molecules are responsible for the obtained activities. However, further studies focusing on the isolation and the determination of antioxidant and antidiabetic effects of F. vulgare rootstock and leaf main compounds are required.

scholarly journals Determination of total phenolic content and antioxidant activity of fruit mix from Malino, Gowa Regency, South Sulawesi

2021 ◽  
Vol 911 (1) ◽  
pp. 012085
Author(s):  
Risma Eva Cahyanti ◽  
Yusminah Hala ◽  
A. Mu’nisa
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Passion Fruit ◽  
Total Phenolic ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Dpph Method ◽  
Purple Passion Fruit

Abstract This study aimed to determine the total phenolic content and antioxidant activity of single fruit extracts and mixtures of tomatoes (Solanum lycopersicum), purple passion fruit (Passiflora edulis var. Sims), and strawberries (Fragaria sp.). Tomatoes, purple passion fruit and strawberries were extracted using 96% ethanol as solvent using the maceration method. Determination of total phenolic content using the Folin-ciocalteu method, measurement of antioxidant activity using the DPPH method (1.1 diphenyl-2-picrylhydrazil) spectrophotometrically and measuring the degree of acidity (pH) using a pH meter. Data were analyzed using ANOVA with Tukey’s further test. The results showed that the total phenolic content of tomatoes, purple passion fruit and strawberries before and after mixing were tomato extract (1,731 mg GAE/g), purple passion fruit extract (1,577 mg GAE/g), strawberry extract (1,917 mg GAE)./g), tomato and purple passion fruit extract (1,758 mg GAE/g), tomato and strawberry extract (2,020 mg GAE/g), strawberry and purple passion fruit extract (1,924 mg GAE/g) and tomato, purple passion fruit extract and strawberries (2.107 mg GAE/g). The antioxidant activity showed that there was a significant difference between the purple passion fruit treatments (78.695%), tomatoes and strawberries (86.160%) and tomatoes, purple passion fruit and strawberries (88.328%), but not significantly different from the tomatoes (80.683 %), tomatoes and purple passion fruit (82,059 %) as well as the treatment of strawberries (83.690 %), strawberries and purple passion fruit (84.097 %), but significantly different from the BHA control (93.526 %). It can be concluded that the total phenolic content and antioxidant activity of the mixed extracts of the three fruits, namely tomatoes, purple passion fruit and strawberries, were higher than those of the single fruit extracts without mixing.

scholarly journals Antioxidant and Antimicrobial Evaluation and Chemical Investigation of Rosa gallica var. aegyptiaca Leaf Extracts

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6498
Author(s):  
Ahmed S. Abdelbaky ◽  
Abir M. H. A. Mohamed ◽  
Salman S. Alharthi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Flowering Plant ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Rosa Gallica

Rosa gallica var. aegyptiaca is a species of flowering plant belonging to the Rosaceae family that plays an important role as a therapeutic agent for the treatment of specific types of cancer, microbial infections, and diabetes mellitus. This work presents the first report on the evaluation of the antioxidant and antimicrobial potential along with the phytochemical analysis of Rosa gallica var. aegyptiaca leaves. Five leaf extracts of hexane, chloroform, methanol, hydromethanol 80%, and water were prepared. Assessment of antioxidant activity was carried out via DPPH radical scavenging assay. Antimicrobial activity against five foodborne pathogenic bacteria—including Listeria monocytogenes, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Salmonella enteritidis—and the fungus Candida albicans, was examined using the disc diffusion method. Total phenolic content and total flavonoid content were determined using the Folin–Ciocalteu reagent and aluminum chloride methods, respectively. Isolation, identification, and quantification of phenolic compounds were performed using HPLC-DAD analysis. Amongst the five leaf extracts that were investigated, hydromethanol 80% extract possessed the highest extraction yield, antioxidant activity, total phenolic content, and antimicrobial activity against all tested microbial strains. Moreover, this extract furnished six active phenolic compounds: gallic acid (1), (+) catechin (2), chlorogenic acid (3), (–) epicatechin (4), quercetin-3-O-α-d-(glucopyranoside) (5), and quercetin (6). This study provides an alternative utilization of R. gallica var. aegyptiaca leaves as a readily accessible source of natural antioxidants and antimicrobials in the food and pharmaceutical industries.

scholarly journals Roots and Leaf Extracts of Dipsacus fullonum L. and Their Biological Activities

Plants ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 78 ◽  
Author(s):  
Jan Oszmiański ◽  
Aneta Wojdyło ◽  
Piotr Juszczyk ◽  
Paulina Nowicka
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Leaf Extract ◽  
Biological Activities ◽  
Leaf Extracts ◽  
Leaves And Roots

The aim of the study was to identify and evaluate the content of iridoids and phenolic compounds in the leaves and roots of Dipsacus fullonum L. They were identified and quantified by UPLC-PDA-MS/MS. Five iridoid compounds (loganic acid, loganin, sweroside, cantleyoside, and sylvestroside III) were identified in Dipsacus fullonum L. leaves and roots. Seven phenolic acids and three flavones were identified in the leaves, and seven phenolic acids were detected in the roots. The leaves contained more iridoids and phenolic compounds than the roots. We also evaluated the antimicrobial (anti-bacterial and anti-yeast), antioxidant (ORAC methods), and antiacetylcholinesterase (AChE) activities of Dipsacus fullonum L. leaves and roots. Leaf extract demonstrated the strongest antioxidant activity, but roots showed stronger antiacetylcholinesterase activity than leaves. The study also confirmed antibacterial activity of root-derived compounds against Staphylococcus aureus DSM 799 and Escherichia coli ATCC 10536.

Antibacterial, Antifungal and Antioxidant activity of leaf and fruit of Alangium salviifolium L. (Cornaceae)

2021 ◽  
pp. 2745-2749
Author(s):  
Lavanya D ◽  
Pooja Rao ◽  
Soundarya S ◽  
Surabhi T.S ◽  
Prashith Kekuda T.R
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Medicinal Uses ◽  
Fruit Extracts ◽  
The Family ◽  
Reducing Activity ◽  
Antibacterial And Antifungal

Objectives: Alangium salviifolium L. is one of the medicinally important plant belonging to the family Cornaceae. The present study evaluates antibacterial, antifungal and antioxidant activity of leaf and fruit extracts of A. salviifolium. Methods: Extraction of shade dried and powdered leaf and fruit was carried out by maceration technique. Antibacterial and antifungal activity was evaluated by agar well diffusion method and poisoned food technique, respectively. Antioxidant activity was determined by antiradical (DPPH and ABTS assays) and ferric reducing assays. Results: Both leaf and fruit extracts displayed inhibitory activity against test bacteria. Fruit extract was shown to be more effective against test bacteria when compared to leaf extract. Leaf extract was more effective in causing inhibition of mycelial growth of test fungi when compared to fruit extract. Both leaf and fruit extracts scavenged DPPH and ABTS radicals dose dependently and exhibited ferric reducing activity. Conclusions: The results of the present study indicated the potential of leaf as well as fruit of A. salviifolium to exhibit antimicrobial and antioxidant activities which justifies the traditional medicinal uses of the plant.

scholarly journals Antioxidant Activity Test for Rukam Fruit (Flacourtia rukam) Of Maseration Extract

2019 ◽  
Vol 1 (1) ◽  
pp. 14-19
Author(s):  
Inas Fadiyah ◽  
Iin Lestari ◽  
Shelly Victory
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Phenolic Compounds ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Wild Plants ◽  
Fruit Extract ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method

Rukam fruit (Flacourtia rukam) is one of the wild plants that are widely distributed in Indonesia, especially on the island of Bangka. The results of previous studies stated that rukam fruit has a phenolic content of 40 mg GAE / 100 grams. Phenolic compounds have major benefits as antioxidants, where antioxidants have the ability to inhibit the work of free radicals. Therefore a study was conducted to assess the content of secondary metabolites and the antioxidant bioactivity of the Flacourtia rukam species using maceration method. Rukam fruit extract was obtained using the Maceration method. As for the antioxidant test using the DPPH` method. The results of the antioxidant activity test of rukam extract with ethanol solvent obtained IC50 value 236,169 µg / mL and in rukam extract with acetone solvent obtained IC50 276,443 µg / mL which showed that the antioxidant properties of this rukam were moderate.

Determination of Phenolic Compounds’ Extraction Conditions from Pistacia palaestina Leaves at Two Different Stages of Maturity

2020 ◽  
Vol 16 (5) ◽  
pp. 808-814
Author(s):  
Wissam Zam ◽  
Ali Ali ◽  
Razan Hasan
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Growth Stages ◽  
Extraction Solvent ◽  
Antioxidant Agents ◽  
Pistacia Species

Background: Phenolic compounds are distributed throughout the plant kingdom. Numerous investigations have proved that Pistacia species have secondary metabolites, and these contain different classes of bioactive phenolic compounds. Objective: The objective of the present work was to optimize the extraction conditions of phenolic compounds from Pistacia palaestina leaves cultivated in Syria and find out the effects of the solvent type, extraction time, and temperature on total phenolic content (TPC) and in vitro antioxidant activity% (AA%). Two different growth stages were studied. Methods: Antioxidant activity and total phenolic content values were estimated using 2,2’-diphenyl- 1-picrylhydrazyl and Folin-Ciocalteu methods, respectively. Results: The highest TPC and AA% were found in fresh leaves (14.32g/100g and 92.69%, respectively). The optimum extraction solvent for phenolic recovery and antioxidant capacity was acetone 40%. Changes both in temperature (25, 40, 60 and 80°C) and time (15, 30, 45 and 60 min) had no significant influence on TPC and AA% of Pistacia extracts. Conclusion: Phenolic compounds could be extracted in a short time at a low temperature and could be used as natural antioxidant agents.

scholarly journals Bioactive properties of Persea lingue Ness (Lauraceae) fruit and leaf extracts

2023 ◽  
Vol 83 ◽  
Author(s):  
A. Lillo ◽  
F. Carvajal-Caiconte ◽  
W. Vital ◽  
P. I. Silva Junior ◽  
E. J. Paredes-Gamero ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Leaf Extract ◽  
Leukemia Cell ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Fruit Extract ◽  
South Central

Abstract Persea lingue Ness is a tree species that lives mainly in temperate forests of south-central Chile. Its leaves are used in ethnomedicine, the fruit is a drupe similar to that of the avocado and has not been studied. The aim of this study was to determine the cytotoxicity in leukemia cell and antibacterial activity, along with some chemical content characteristics of P. lingue fruit and leaf extracts. The antibacterial activity was determined by the inhibition of bacterial growth in liquid medium assay against Gram-positive and Gram-negative bacteria. The leukemia cell lines Kasumi-1 and Jurkat were used to evaluate the cytotoxic activity by using propidium iodide and AlamarBlue assays. Total phenolic, flavonoid, condensed tannin, alkaloid and lipid contents were evaluated in the fruit and in the leaf extracts. The antioxidant activity of both extracts were also elavaluated. Leaf extract presented the highest content of total phenols, condensed tannins and flavonoids, and also the highest antioxidant activity. While the fruit extract has a higher amount of lipids and alkaloids and the high antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus megaterium and Micrococcus luteus. The leaf extract only showed activity against M. luteus. Concerning the cytotoxic activity, only the fruit extract showed cytotoxicity against the cell lines Jurkat and Kasumi-1. P. lingue fruit extract is a potential source of biologically active molecules for the development of new drugs to be used in some types of leukemia, as well as antibacterial agent.

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