Analyse des Vermehrungsmechanismus des Newcastle disease Virus (NDV) mit Hilfe verschiedener Inhibitoren
The reproduction of NDV in chick-embryo-fibroblast cultures was studied with 6-Azauridine, 8-Azaguanine, Parafluorophenylalanine (FPA) and Puromycine as inhibitors. The results suggest that no virus initiated FPA-sensitive material is needed for the uncoating of the infecting particles, and that viral parental RNA is able to induce the formation of protein (s) needed for viral RNA-synthesis (“RNA-protein“) as well as the production of viral structural antigen (s). Further antigenic material appears after the beginning of new viral RNA-synthesis. The “RNA-protein (s)“become (s) detectable between 2 and 3 hours after infection and is (are) stable in its function over several hours. According to the formation of viral antigenic material parental viral RNA can act as a messenger longer than 9 hours. The capacity for the production of hemagglutinating units appears after the viral antigen producing capacity, when viral RNA can already be synthesized. This capacity is separated from that to produce plaque forming particles by a FPA-sensitive phase. The character of the corresponding FPA-sensititve material is unknown.