Potent Free Radical Scavenging Activity of Propol Isolated from Brazilian Propolis

1997 ◽  
Vol 52 (11-12) ◽  
pp. 828-833 ◽  
Author(s):  
Purusotam Basnet ◽  
Tetsuya Matsuno ◽  
Richard Neidlein
Keyword(s):  
Free Radical ◽  
Vitamin E ◽  
Superoxide Anion ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Water Extract ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Brazilian Propolis

Abstract We evaluated free radical scavenging activity of the water, methanol and chloroform ex­tracts of propolis in 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and xanthine-xanthine oxidase (XOD) generated superoxide anion assay systems. The free radical scavenging activity guided fractionation and chemical analysis led to the isolation of a new compound, propol {3-[4-hydroxy-3-(3-oxo-but-1-enyl)-phenyl]-acrylic acid) from the water extract, which was more potent than most common antioxidants such as vitamin C and vitamin E (α-tocopherol) in these assay systems.

Bioactive Composition of Pig Laryngeal Cartilage Extracts and Their Free Radical-scavenging Activity

2006 ◽  
Vol 12 (5) ◽  
pp. 371-377 ◽  
Author(s):  
Shuang-li Xiong ◽  
Zheng-yu Jin ◽  
An-lin Li
Keyword(s):  
Free Radical ◽  
Amino Acid ◽  
Superoxide Anion ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Laryngeal Cartilage ◽  
Hydroxyl Free Radical

This work focused on amino acid and mineral element analysis of pig laryngeal cartilage. Papain and DEAE-Sepharose fast flow ion exchange chromatography were used to hydrolyase cartilage and purify hydrolysate. Further study was performed to compare the free radical-scavenging activity of various fractions. Cartilage is abundant in bioactive amino acids such as glycine, glutamic acid and arginine and minerals such as calcium, magnesium and iron. Four polysaccahrides attached peptides and one peptide were isolated from hydrolysate. Weak 2,2-diphenyl-1-picryhydrazyl free radical scavenging activities of all substances were observed. Other fractions showed hydroxyl free radical and superoxide anion free radical scavenging ability in a concentration-dependent manner except fraction A. The hydroxyl free radical scavenging activity of tested samples decreased in the following order: fraction C crude chondroitin sulphate (ChS) peptide fraction B semi-purified ChS purified ChS. Significant differences were observed between purified ChS and other fractions (P 0.01). For superoxide anion free radical the order was: peptide purified ChS semi-purified ChS crude ChS fraction C fraction B. The peptide was significantly higher than purified ChS (P 0.01), which was significantly higher than the other components (P 0.01). According to the results, polysaccharides, ChS, amino acid and peptide present in cartilage had important bioactivity.

EVALUATION OF FREE RADICAL SCAVENGING ACTIVITY OF VOLVARIELLA (Volvariella volvacea), WOOD EAR (Auricularia auricula-judae), AND SHIITAKE (Lentinula edodes) MUSHROOMS

2017 ◽  
Vol 11 (1) ◽  
pp. 91-101
Author(s):  
JEBBEN PEREZ ◽  
◽  
CANDELARIO CALIBO ◽  
Keyword(s):  
Free Radical ◽  
Lentinula Edodes ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Water Extract ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Volvariella Volvacea ◽  
Auricularia Auricula

Edible mushrooms, whether fresh or processed, are widely consumed worldwide. In order to determine their nutritional and/or functional values, this study was conducted to evaluate the free radical scavenging activity of Volvariella (Volvariella volvacea), wood ear (Auricularia auricula-judae), and shiitake (Lentinula edodes) mushrooms. Trolox equivalent per 100g sample (TE/100g) was determined to describe the activity using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Results showed that wood ear exhibited the highest free radical scavenging activity (387.11±30.26 TE/100g) among the three species. Shiitake mushroom exhibited 368.13 ±27.03 TE/100g activity. Volvariella contained the lowest activity with 318.72 ±36.87 TE/100g. Based on the solvent system, ethanolic extracts showed the highest free radical scavenging activity (386.45±23.30 TE/100g) while hexane extract showed the least free radical scavenging activity (326.34 ±27.75 TE/100g). Water extract exhibited 361.17 ±49.11TE/100g activity. The results of this study showed that mushroom extracts could serve as natural antioxidants due to their significant antioxidative property

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