Anti-Inflammatory and Antinociceptive Activity of Flavonoids Isolated from Viscum album ssp. album

Abstract Viscum album L. has been used in the indigenous systems of medicine for treatment of headache and some inflammatory diseases. In order to evaluate this information, antinociceptive and anti-inflammatory activities of the five flavonoids (5,7-dimethoxy naringenin or 4′,6′- dimethoxy chalcononaringenin) derivatives, isolated from the ethyl acetate fraction of the extract from V. album ssp. album, were investigated, namely 5,7-dimethoxy-flavanone-4′-O- β-ᴅ-glucopyranoside (1), 2′-hydroxy-4′,6′-dimethoxy-chalcone-4-O-β-ᴅ-glucopyranoside (2), 5,7-dimethoxy-flavanone-4′-O-[2″-O-(5‴-O-trans-cinnamoyl)-β-ᴅ-apiofuranosyl]-β-ᴅ-glucopyranoside (3), 2′-hydroxy-4′,6′-dimethoxy-chalcone-4-O-[2″-O-(5‴-O-trans-cinnamoyl)-β-ᴅ-apiofuranosyl]- β-ᴅ-glucopyranoside (4), 5,7-dimethoxy-flavanone-4′-O-[β-d-apiofuranosyl- (152)]-β-ᴅ-glucopyranoside (5). For the antinociceptive activity assessment the p-benzoquinone- induced writhing test and for the anti-inflammatory activity the carrageenan-induced hind paw edema model in mice were used. The ethyl acetate fraction in a dose of 250 mg/kg as well as compounds 2 and 5 in a 30 mg/kg dose were shown to possess remarkable antinociceptive and anti-inflammatory activities per os without inducing any apparent acute toxicity as well as gastric damage

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Evaluation of in vivo Biological Activity Profile of Isoorientin

Zeitschrift für Naturforschung C ◽

10.1515/znc-2004-11-1204 ◽

2004 ◽

Vol 59 (11-12) ◽

pp. 787-790 ◽

Cited By ~ 44

Author(s):

Esra Küpeli ◽

Mustafa Aslan ◽

İlhan Gürbüz ◽

Erdem Yesilada

Keyword(s):

Biological Activity ◽

Paw Edema ◽

Gastroprotective Activity ◽

Activity Profile ◽

Writing Test ◽

Activity Assessment ◽

Edema Model ◽

Anti Inflammatory ◽

Rats And Mice

Anti-nociceptive, anti-inflammatory and gastroprotective activities of the known C-glycosyl flavonoid, isoorientin, were studied in rats and mice. For the anti-nociceptive activity assessment the p-benzoquinone-induced writing test, for the anti-inflammatory activity the carrageenan-induced hind paw edema model in mice, and for the gastroprotective activity the EtOH-induced ulcerogenesis model in rats were used. Isoorientin was shown to possess significant anti-nociceptive and anti-inflammatory activities at 15 mg/kg and 30 mg/kg doses, without inducing any apparent acute toxicity as well as gastric damage. However, the compound did not possess any significant gastroprotective activity against EtOH-induced ulcerogenesis.

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Leaves of Stereospermum suaveolens DC Exhibit Anti-inflammatory and Anti-arthritic Potential Action in Experimental Animals

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i33a31783 ◽

2021 ◽

pp. 164-175

Author(s):

R. R. Chanshetti ◽

D. D. Bandawane

Keyword(s):

Ethyl Acetate ◽

Methanolic Extract ◽

Research Work ◽

Ethyl Acetate Fraction ◽

Significant Inhibition ◽

Paw Edema ◽

Standard Drug ◽

Rat Paw Edema ◽

Anti Inflammatory ◽

Rat Paw

Aim: The experimental investigation of current research work was to identify traditional rich claim of Stereospermum suaveolens DC leaves for anti-inflammatory and anti-arthritic potential action in animals. Study design: Ethyl acetate fraction of Stereospermum suaveolens DC (Bignoniaceae) methanolic extract of leaves evaluated at 125mg/kg, 250mg/kg and 500mg/kg (p.o.) doses for anti-inflammatory and anti-arthritic activity. Methodology: Ethyl acetate fraction of Stereospermum suaveolens DC (Bignoniaceae) methanolic extract of leaves was evaluated for phytochemical investigation for total flavonoid content using UV spectroscopy and TLC study. Carrageenan induced rat paw edema (Acute method) and Freund’s complete adjuvant (FCA) induced chronic arthritis in wistar rats were used as an animal models to claim Stereospermum suaveolens DC leaves for anti-inflammatory and anti-arthritic potential. The rat paw volume and percentage inhibition of the paw edema were evaluated for anti-inflammatory activity. The assessments of arthritis in rats were measured by haematological values and radiological examinations. Result: Ethyl acetate fraction of Stereospermum suaveolens DC (Bignoniaceae) methanolic extract of leaves showed presence of total flavonoids and saponins. The significant inhibition in paw volume and edema (p < .01) obtained at 250mg/kg and 500mg/kg oral dose. These obtained results were established confirmation outcome for presence of rich flavonoid contents in Stereospermum suaveolens DC leaves and provides valuable source of bioactive phytocomponents. Conclusion: Ethyl acetate fraction of Stereospermum suaveolens DC (Bignoniaceae) methanolic extract of leaves showed significant inhibition of inflammatory reaction as compared to standard drug indomethacin Sterospermum Suaveolens DC leaves were showed potential therapeutic role in treatment of inflammation and arthritis cases.

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PHYTOCHEMICAL SCREENING AND ANTI-INFLAMMATORY ACTIVITY OF FRACTIONS FROM SAMBUNG RAMBAT (MICANIA CORDATA) LEAF

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i8.26083 ◽

2018 ◽

Vol 11 (8) ◽

pp. 199

Author(s):

Zola Efa Harnis ◽

Panal Sitorus ◽

Rosidah Rosidah

Keyword(s):

Ethyl Acetate ◽

Screening Method ◽

Ethyl Acetate Fraction ◽

Inflammatory Activity ◽

Phytochemical Screening ◽

Paw Edema ◽

Water Solvent ◽

Phytochemical Compounds ◽

Anti Inflammatory

Objective: The present study is to evaluate the anti-inflammatory activity of various fractions from Sambung Rambat leaf (Micania cordata) using ethyl acetate, hexane, and water solvent as the solvent.Methods: Investigation of phytochemical compound was using standard phytochemical screening method, while the anti-inflammatory was using the carrageenan-induced paw edema method in animal (rats) model.Results: The phytochemical screening showed that the fractions of M. cordata had a lot of phytochemical compounds such as flavonoids, glycosides, steroids, triterpenoids, tannins, and saponins. Anti-inflammatory assessment showed that the strongest activity of anti-inflammatory produced by ethyl acetate fraction with 200 mg/kg BW dose.Conclusion: This investigation could be concluded that ethyl acetate fraction of M. cordata might be a potential for the treatment of inflammatory.

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Anti-Inflammatory and Antinociceptive Potential of Major Phenolics from Verbascum salviifolium Boiss

Zeitschrift für Naturforschung C ◽

10.1515/znc-2008-3-406 ◽

2008 ◽

Vol 63 (3-4) ◽

pp. 196-202 ◽

Cited By ~ 19

Author(s):

I. Irem Tatli ◽

Zeliha S. Akdemir ◽

Erdem Yesilada ◽

Esra Küpeli

Keyword(s):

Inflammatory Activity ◽

Antinociceptive Activity ◽

Paw Edema ◽

Spectral Techniques ◽

Ear Edema ◽

Aerial Parts ◽

Edema Model ◽

Bioassay Guided Fractionation ◽

Anti Inflammatory ◽

Dehydrodiconiferyl Alcohol

The potential effects of flavonoids, phenylethanoid and neolignan glycosides from the aerial parts of Verbascum salviifolium Boiss. were studied in the p-benzoquinone-induced writhing reflex, for the assessment of the antinociceptive activity, and in carrageenan- and PGE1- induced hind paw edema and 12-O-tetradecanoyl-13-acetate (TPA)-induced ear edema models in mice, for the assessment of the anti-inflammatory activity. Through bioassay-guided fractionation and isolation procedures ten compounds from the aqueous extract of the plant, luteolin 7-O-glucoside (1), luteolin 3′-O-glucoside (2), apigenin 7-O-glucoside (3), chrysoeriol 7-O-glucoside (4), β-hydroxyacteoside (5), martynoside (6), forsythoside B (7), angoroside A (8), dehydrodiconiferyl alcohol-9-O′-d-glucopyranoside (9) and dehydrodiconiferyl alcohol- 9′-O-β-d-glucopyranoside (10), were isolated and their structures were elucidated by spectral techniques. Results have shown that 1, 2, 3 and 5 significantly inhibited carrageenaninduced paw edema at a 200 mg/kg dose, while 1, 2 and 5 also displayed anti-inflammatory activity against the PGE1-induced hind paw edema model. However, all the compounds showed no effect in the TPA-induced ear edema model. The compounds 1 and 2 also exhibited significant antinociceptive activity

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Anti-Inflammatory and Antinociceptive Activity of Coumarins from Seseli gummiferum subsp. corymbosum (Apiaceae)

Zeitschrift für Naturforschung C ◽

10.1515/znc-2009-1-210 ◽

2009 ◽

Vol 64 (1-2) ◽

pp. 56-62 ◽

Cited By ~ 9

Author(s):

Alev Tosun ◽

Esra Küpeli Akkol ◽

Erdem Yeşilada

Keyword(s):

Ethyl Acetate ◽

Coumarin Derivatives ◽

Paw Edema ◽

Aerial Parts ◽

Edema Model ◽

Mouse Ear ◽

Anti Inflammatory ◽

Mouse Ear Edema ◽

Ethyl Acetate Extracts

1n-Hexane and ethyl acetate extracts as well as coumarin derivatives obtained from the n-hexane extract of the aerial parts of Seseli gummiferum Pall. ex Sm. subsp. corymbosum (Boiss. & Heldr.) P.H. Davis (Apiaceae) were evaluated in vivo for their anti-inflammatory and antinociceptive activities. The n-hexane and ethyl acetate extracts of the species were shown to possess significant inhibitory activity against the carrageenan-induced hind paw edema and p-benzoquinone-induced writhing models in mice. Among the isolated coumarin derivatives; (−)-(3’S,4’S)-3’-acetoxy-4’-isovaleryloxy-3’,4’-dihydroseselin (), (−)-(3’S,4’S)-3’- acetoxy-4’-angeloyloxy-3’,4’-dihydroseselin (2), (+)-(3’S,4’S)-3’-hydroxy-4’-angeloyloxy-3’,4’- dihydroseselin (D-laserpitin) (3), (−)-(3’S,4’S)-3’-angeloyloxy-4’-hydroxy-3’,4’-dihydroseselin (4), and osthole (5), only the 3’-acetoxy derivatives 1 and 2 were found to possess potent antinociceptive and anti-infl ammatory activities, per os, without inducing any apparent acute toxicity as well as gastric damage, while all other compounds and extracts were found to be ineffective in the TPA-induced mouse ear edema model assay.

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Anti-inflammatory effect of leaves of Vernonia zeylanica in lipopolysaccharide-stimulated RAW 264.7 macrophages and carrageenan-induced rat paw-edema model

Journal of Ethnopharmacology ◽

10.1016/j.jep.2021.114030 ◽

2021 ◽

pp. 114030

Author(s):

Dilani Rukshala ◽

E. Dilip de Silva ◽

B.V.Loshini R. Ranaweera ◽

Narmada Fernando ◽

Shiroma M. Handunnetti

Keyword(s):

Raw 264.7 ◽

Paw Edema ◽

Raw 264.7 Macrophages ◽

Rat Paw Edema ◽

Edema Model ◽

Anti Inflammatory ◽

Rat Paw ◽

Inflammatory Effect

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Ethanolic Extracts of Adlay Testa and Hull and Their Active Biomolecules Exert Relaxing Effect on Uterine Muscle Contraction through Blocking Extracellular Calcium Influx in Ex Vivo and In Vivo Studies

Biomolecules ◽

10.3390/biom11060887 ◽

2021 ◽

Vol 11 (6) ◽

pp. 887

Author(s):

Yun-Ju Huang ◽

Yu-Chieh Chen ◽

Hsin-Yuan Chen ◽

Yi-Fen Chiang ◽

Mohamed Ali ◽

...

Keyword(s):

Ethyl Acetate ◽

Uterine Contraction ◽

Calcium Influx ◽

Ex Vivo ◽

Ethanolic Extract ◽

Ethyl Acetate Fraction ◽

In Vivo Studies ◽

Human Myometrium ◽

Writhing Test

Dysmenorrhea is one of the most prevalent disorders in gynecology. Historically, adlay (Coix lachryma-jobi L. var. Ma-yuen Stapf.) has been explored for its anti-tumor, pain relief, anti-inflammatory, and analgesic effects. The aim of this study was to evaluate the effects of adlay seeds on the inhibition of uterine contraction and thus dysmenorrhea relief, in vitro and in vivo. HPLC-MS and GC were used to elucidate the ethyl acetate fraction of adlay testa ethanolic extract (ATE-EA) and ethyl acetate fraction of adlay hull ethanolic extract (AHE-EA). Elucidation yielded flavonoids, phytosterols, and fatty acids. Uterine leiomyomas and normal adjacent myometrial tissue were evaluated by oxytocin- and PG-induced uterine contractility. ATE-EA and AHE-EA suppressed uterine contraction induced by prostaglandin F2 alpha (PGF2α), oxytocin, carbachol, and high-KCl solution ex vivo. In addition, the external calcium (Ca2+) influx induced contraction, and increased Ca2+ concentration was inhibited by ATE-EA and AHE-EA on the uterine smooth muscle of rats. Furthermore, ATE-EA and AHE-EA effectively attenuated the contraction of normal human myometrium tissues more than adjacent uterine leiomyoma in response to PGF2α. 3,5,6,7,8,3′,4′-Heptamethoxyflavone and chrysoeriol produced a remarkable inhibition with values of IC50 = 24.91 and 25.59 µM, respectively. The experimental results showed that treatment with ATE-EA at 30 mg/day effectively decreased the writhing frequency both on the oxytocin-induced writhing test and acetic acid writhing test of the ICR mouse.

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SYNTHESIS AND PRELIMINARY PHARMACOLOGICAL EVALUATION OF NEW NAPROXEN ANALOGUES HAVING 1, 2, 4-TRIAZOLE-3-THIOL

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i7.18273 ◽

2017 ◽

Vol 9 (7) ◽

pp. 66

Author(s):

Monther F. Mahdi ◽

Noor H. Naser ◽

Nethal H. Hammud

Keyword(s):

Aromatic Aldehydes ◽

Heterocyclic Ring ◽

Inflammatory Activity ◽

Control Group ◽

Paw Edema ◽

Dose Equivalent ◽

Edema Model ◽

Anti Inflammatory ◽

Derivatives Of

Objective: The objective of this search was to synthesize a new naproxen analogues having a 1,2,4-triazole-3-thiol heterocyclic ring, and preliminary pharmacological assessment of the anti-inflammatory activity of the synthesized compounds. Methods: The synthesis of naproxen analogues that having 1,2,4-triazole-3-thiol heterocyclic ring occur through esterification of naproxen, and then its reaction with hydrazine hydrate, and carbon disulfide, finally different aromatic aldehydes reacted with triazole derivatives of naproxen containing amino group to produce schiff bases.Results: In vivo acute anti-inflammatory activity of the synthesize compounds (Va-Vd) was evaluated in rats using egg-white induced edema model of inflammation in a dose equivalent to (50 mg/kg) of naproxen. All tested compounds were produced a significant reduction in paw edema with respect to the effect of propylene glycol 50% v/v (control group). Compound Vd produced superior anti-inflammatory activity compared to naproxen.Conclusion: The results obtained in this work give evidence about the valid synthesis of 1,2,4 triazole-3-thiol derivatives of naproxen, which reacted with different aldehydes to yield several schiff bases. The incorporation of benzaldehyde possess para-electron donating group (para-hydroxyl benzaldehyde) will increase the anti-inflammatory activity of naproxen.

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