Test Method for the Automated Determination of Refractive Index of Glass Samples Using the Oil Immersion Method and a Phase Contrast Microscope

2019 ◽  
Author(s):  
Keyword(s):  
Refractive Index ◽  
Phase Contrast ◽  
Test Method ◽  
Immersion Method ◽  
Phase Contrast Microscope ◽  
Oil Immersion ◽  
Contrast Microscope ◽  
Automated Determination

Histochemical and Morphological Studies of Lipids in the Oogenesis of Pheretima posthuma

1958 ◽  
Vol s3-99 (48) ◽  
pp. 475-484
Author(s):  
VISHWA NATH ◽  
BRIJ L. GUPTA ◽  
S. L. MANOCHA
Keyword(s):  
Phase Contrast ◽  
Chemical Change ◽  
Follicular Epithelium ◽  
Cholesteryl Esters ◽  
Lipid Bodies ◽  
Interference Microscope ◽  
Phase Contrast Microscope ◽  
Morphological Studies ◽  
Contrast Microscope ◽  
Second Category

A study of the oocytes of the earthworm, Pheretima posthuma, examined fresh under the phase-contrast and interference microscopes as well as by histochemical techniques, has revealed that there are two types of lipid bodies in the cytoplasm. The lipid bodies of the first type (L1) are smaller, appear as homogeneous, dark granules under the phase-contrast microscope, and have a protein-phospholipid core surrounded by a thick sheath of phospholipids only. The lipid bodies of the second category (L2), which arise as a result of growth and chemical change in L1 bodies, have a pure phospholipid core surrounded by a thick triglyceride sheath. They give a ringed appearance under the phase-contrast microscope. The study under the interference microscope shows that this ringed appearance is an optical artifact. The lipid spheres present in the follicular epithelium contain phospholipids only. The mitochondria are in the form of minute granules. They remain unchanged throughout oogenesis. Some vacuoles devoid of any lipids, proteins, or carbohydrates have been observed. They also remain unchanged. Pure triglyceride spheres, yolk globules, nucleolar extrusions, as well as cholesterols and cholesteryl esters are absent.

Redescription of Neelus fimbriatus Bretfeld & Trinklein, 2000 (Collembola: Neelidae) from Colombia

Zootaxa ◽  
2018 ◽  
Vol 4527 (3) ◽  
pp. 414
Author(s):  
ELIDA P. MARÍN ◽  
JOSÉ G. PALACIOS-VARGAS
Keyword(s):  
Phase Contrast ◽  
Phase Contrast Microscope ◽  
Contrast Microscope

Neelus fimbriatus is redescribed using specimens from Colombia. Drawings and phase contrast microscope photos of the species are used. New characters are used as tibiotarsal tuberculate setae and abdominal ventral acetabula. 

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