scholarly journals Are there any differences between demographic characteristics, preoperative ultrasonographic findings, and cytological results of patients with thyroid tumors of uncertain malignant potential and papillary thyroid carcinoma of classical and non-encapsulated follicular variants?

2016 ◽  
Author(s):  
Husniye Baser ◽  
Oya Topaloglu ◽  
Abbas Ali Tam ◽  
Afra Alkan ◽  
Aydan Kilicarslan ◽  
...  
Keyword(s):  
Papillary Thyroid Carcinoma ◽  
Thyroid Carcinoma ◽  
Demographic Characteristics ◽  
Malignant Potential ◽  
Thyroid Tumors ◽  
Papillary Thyroid ◽  
Uncertain Malignant Potential

Identification of Differentially Expressed Genes Associated with Papillary Thyroid Carcinoma

2020 ◽  
Vol 23 (6) ◽  
pp. 546-553
Author(s):  
Hongyuan Cui ◽  
Mingwei Zhu ◽  
Junhua Zhang ◽  
Wenqin Li ◽  
Lihui Zou ◽  
...  
Keyword(s):  
Papillary Thyroid Carcinoma ◽  
Thyroid Carcinoma ◽  
Differentially Expressed Genes ◽  
Molecular Mechanisms ◽  
Cellular Proliferation ◽  
Mrna Level ◽  
Thyroid Tissue ◽  
Differentially Expressed ◽  
Thyroid Tumors ◽  
Papillary Thyroid

Objective: Next-generation sequencing (NGS) was performed to identify genes that were differentially expressed between normal thyroid tissue and papillary thyroid carcinoma (PTC). Materials & Methods: Six candidate genes were selected and further confirmed with quantitative real-time polymerase chain reaction (qRT-PCR), and immunohistochemistry in samples from 24 fresh thyroid tumors and adjacent normal tissues. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was used to investigate signal transduction pathways of the differentially expressed genes. Results: In total, 1690 genes were differentially expressed between samples from patients with PTC and the adjacent normal tissue. Among these, SFRP4, ZNF90, and DCN were the top three upregulated genes, whereas KIRREL3, TRIM36, and GABBR2 were downregulated with the smallest p values. Several pathways were associated with the differentially expressed genes and involved in cellular proliferation, cell migration, and endocrine system tumor progression, which may contribute to the pathogenesis of PTC. Upregulation of SFRP4, ZNF90, and DCN at the mRNA level was further validated with RT-PCR, and DCN expression was further confirmed with immunostaining of PTC samples. Conclusion: These results provide new insights into the molecular mechanisms of PTC. Identification of differentially expressed genes should not only improve the tumor signature for thyroid tumors as a diagnostic biomarker but also reveal potential targets for thyroid tumor treatment.

Proteomic Study of Thyroid Tumors Reveals Frequent Up-Regulation of the Ca2+-Binding Protein S100A6 in Papillary Thyroid Carcinoma

Thyroid ◽  
2010 ◽  
Vol 20 (10) ◽  
pp. 1067-1076 ◽  
Author(s):  
Anastasios Sofiadis ◽  
Andrii Dinets ◽  
Lukas M. Orre ◽  
Rui M. Branca ◽  
Carl Christofer Juhlin ◽  
...  
Keyword(s):  
Papillary Thyroid Carcinoma ◽  
Thyroid Carcinoma ◽  
Binding Protein ◽  
Thyroid Tumors ◽  
Papillary Thyroid ◽  
Proteomic Study

The enucleation of thyroid tumors indeterminate before surgery as papillary thyroid carcinoma: Should immediate reoperation be performed?

Surgery Today ◽  
1994 ◽  
Vol 24 (4) ◽  
pp. 305-308 ◽  
Author(s):  
Kiminori Sugino ◽  
Kunihiko Ito ◽  
Takashi Mimura ◽  
Osamu Ozaki ◽  
Michikazu Kawano ◽  
...  
Keyword(s):  
Papillary Thyroid Carcinoma ◽  
Thyroid Carcinoma ◽  
Thyroid Tumors ◽  
Papillary Thyroid ◽  
Immediate Reoperation

scholarly journals Decreased apolipoprotein A4 and increased complement component 3 as potential markers for papillary thyroid carcinoma: A proteomic study

2018 ◽  
Vol 33 (4) ◽  
pp. 455-462 ◽  
Author(s):  
Reyhaneh Farrokhi Yekta ◽  
Afsaneh Arefi Oskouie ◽  
Mostafa Rezaei Tavirani ◽  
Mohammad R. Mohajeri-Tehrani ◽  
Ahmad R. Soroush
Keyword(s):  
Thyroid Cancer ◽  
Papillary Thyroid Carcinoma ◽  
Thyroid Carcinoma ◽  
Papillary Thyroid Cancer ◽  
Complement Component ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Thyroid Tumors ◽  
Papillary Thyroid ◽  
Complement Component 3

Background: Thyroid carcinomas have comprised the fastest rising incidence of cancer in the past decade. Currently, the diagnosis of thyroid tumors is performed by the fine-needle aspiration biopsy (FNAB) method, which still holds some challenges and limitations, mostly in discriminating malignant and benign lesions. Therefore, the development of molecular markers to distinguish between these lesion types are in progress. Methods: A 2D-PAGE separation of proteins was performed followed by tandem mass spectrometry with the aim of discovering potential serum protein markers for papillary thyroid carcinoma and multinodular goiter. Protein–protein interaction network analysis revealed the most important pathways involved in the progression of papillary thyroid cancer. The enzyme-linked immunosorbent assay method was used to confirm a part of the results. Results: The significantly altered proteins included C3, C4A, GC, HP, TTR, APOA4, APOH, ORM2, KRT10, AHSG, IGKV3-20, and IGKC. We also confirmed that increased complement component 3 and decreased apolipoprotein A4 occurred in papillary thyroid cancer. Network investigations demonstrated that complement activation cascades and PPAR signaling might play a role in the pathogenesis of thyroid cancer. Conclusion: The results demonstrated that serum proteomics could serve as a viable method for proposing novel potential markers for thyroid tumors. Surely, further research must be performed in larger cohorts to validate the results.

scholarly journals Validation of Reference Genes for Normalization of Relative qRT-PCR Studies in Papillary Thyroid Carcinoma

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
S. Adeleh Razavi ◽  
Mandana Afsharpad ◽  
Mohammad Hossein Modarressi ◽  
Maryam Zarkesh ◽  
Parichehreh Yaghmaei ◽  
...  
Keyword(s):  
Papillary Thyroid Carcinoma ◽  
Thyroid Carcinoma ◽  
Internal Control ◽  
Reference Genes ◽  
Thyroid Tissue ◽  
Optimal Number ◽  
Thyroid Tumors ◽  
Papillary Thyroid ◽  
Tissue Samples ◽  
Qrt Pcr

Abstract Quantitative reverse transcription polymerase chain reaction (qRT-PCR) in thyroid tumors require accurate data normalization, however, there are no sufficient studies addressing the suitable reference genes for gene expression analysis in malignant and normal thyroid tissue specimens. The purpose of this study was to identify valid internal control genes for normalization of relative qRT-PCR studies in human papillary thyroid carcinoma tissue samples. The expression characteristics of 12 candidate reference genes (GAPDH, ACTB, HPRT1, TBP, B2M, PPIA, 18SrRNA, HMBS, GUSB, PGK1, RPLP0, and PGM1) were assessed by qRT-PCR in 45 thyroid tissue samples (15 papillary thyroid carcinoma, 15 paired normal tissues and 15 multinodular goiters). These twelve candidate reference genes were selected by a systematic literature search. GeNorm, NormFinder, and BestKeeper statistical algorithms were applied to determine the most stable reference genes. The three algorithms were in agreement in identifying GUSB and HPRT1 as the most stably expressed genes in all thyroid tumors investigated. According to the NormFinder software, the pair of genes including ‘GUSB and HPRT1’ or ‘GUSB and HMBS’ or ‘GUSB and PGM1’ were the best combinations for selection of pair reference genes. The optimal number of genes required for reliable normalization of qPCR data in thyroid tissues would be three according to calculations made by GeNorm algorithm. These results suggest that GUSB and HPRT1 are promising reference genes for normalization of relative qRT-PCR studies in papillary thyroid carcinoma.

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