scholarly journals Cryopreservation of Canine Erythrocytes Using Dimethyl Sulfoxide, Polyethylene Glycol and Sucrose

2021 ◽  
Vol 31 (1) ◽  
pp. 38-50
Author(s):  
Оlga Denysova ◽  
◽  
Gennadiy Zhegunov ◽  
Keyword(s):  
Polyethylene Glycol ◽  
Dimethyl Sulfoxide ◽  
Salt Concentration ◽  
Cryoprotective Agents ◽  
Long Term Storage ◽  
Osmotic Stability ◽  
Medium Increase ◽  
Term Storage ◽  
Saline Medium

Cryoprotective properties of combined media of permeable (dimethyl sulfoxide) and impermeable (polyethylene glycol with m. w. 1500) cryoprotective agents during rapid cooling in liquid nitrogen of canine erythrocytes using saline and sucrose-saline media have been investigated. It was found that the use of combined solutions of cryoprotective agents based on polyethylene glycol with m.w. 1500 (15%) and dimethyl sulfoxide (2.5–10%) in saline was not quite effective for cryopreservation of canine erythrocytes. Reducing the salt concentration and adding cell-impermeable sucrose to the cryopreservation medium increase the preservation of erythrocytes after warming. The best cryoprotective properties for canine erythrocytes were demonstrated by 10% dimethyl sulfoxide based on sucrose-saline medium, with high preservation of cells after freeze-warming, mechanical and osmotic stability of warmed erythrocytes. This indicates the possibility of a long-term storage and use of cryopreserved canine erythrocytes for transfusions.

scholarly journals Preservation of Avian Cells at Sub-zero Temperatures

1979 ◽  
Vol 32 (5) ◽  
pp. 475 ◽  
Author(s):  
N Ratnamohan ◽  
PB Spradbrow
Keyword(s):  
Dimethyl Sulfoxide ◽  
Cryoprotective Agent ◽  
Cryoprotective Agents ◽  
Long Term Storage ◽  
Low Concentrations ◽  
Short And Long Term ◽  
Term Storage

The cryoprotective agents dimethyl sulfoxide (DMSO), glycerol, polyvinylpyrrolidone (PVP) and dextran were evaluated for their ability to protect avian cells during storage at sub-zero temperatures. DMSO was the most effective cryoprotective agent for the short- and long-term storage of avian cells and glycerol was also effective when used at low concentrations. PVP and dextran did not protect avian cells during storage in our experiments. Primary chicken cells and avian cells at higher passage levels were successfully recovered after storage with DMSO for periods ranging from 4 to 12 months.

Comparative analysis of the influence of cryoprotective agents and a long-term storage on human Adipose tissue-Mesenchimal Stromal Cells

Cytotherapy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. S100
Author(s):  
O. Espinosa Ibáñez ◽  
A. Fernández-González ◽  
Á. Sierra-Sánchez ◽  
J. Guerrero ◽  
N. Fernández-Porcel ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Comparative Analysis ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
Cryoprotective Agents ◽  
Long Term Storage ◽  
Term Storage

Long-term storage at −80°C of hematopoietic progenitor cells with 5-percent dimethyl sulfoxide as the sole cryoprotectant

Transfusion ◽  
1999 ◽  
Vol 39 (1) ◽  
pp. 70-73 ◽  
Author(s):  
Antonio Galmés ◽  
Judy Besalduch ◽  
Judy Bargay ◽  
Andres Novo ◽  
Miguel Morey ◽  
...  
Keyword(s):  
Dimethyl Sulfoxide ◽  
Progenitor Cells ◽  
Hematopoietic Progenitor Cells ◽  
Hematopoietic Progenitor ◽  
Long Term Storage ◽  
Term Storage

scholarly journals Novel Approaches to the Cryopreservation of Human Spermatozoa: History and Development of the Spermatozoa Vitrification Technology

2011 ◽  
Vol 2 (2) ◽  
pp. 128-145 ◽  
Author(s):  
Evgenia Isachenko ◽  
Gohar Rahimi ◽  
Peter Mallmann ◽  
Raul Sanchez ◽  
Vladimir Isachenko
Keyword(s):  
Ice Crystals ◽  
Intracellular Lipids ◽  
Cryoprotective Agents ◽  
Long Term Storage ◽  
Cryopreservation Techniques ◽  
Water Quality And Quantity ◽  
And Storage ◽  
Term Storage ◽  
Conventional Freezing

Cryobiology is very intensively applied in reproductive and veterinary medicine for preservation of gametes, embryos and reproductive tissues. Sub-zero temperatures combined with appropriate cryoprotective agents preserve the physiological and reproductive functions of the cells making long-term storage possible without loss of viability. With the use of cryoprotective agents it has become possible to develop cryopreservation techniques, such as the slow conventional freezing and vitrification that are in use in the present times. In slow controlled-rate conventional freezing extracellular ice crystals are formed whereas in vitrification no ice crystals are formed. Glass formation is compatible with the survival of the cell and the preservation of its intracellular structures provided the type(s) and concentrations of cryoprotectant used are not chemo- or osmotoxic. However, irrespective of the type of cooling method employed the cryosurvival of cells and tissues is influenced by the size and maturity of cells, amounts of intracellular water, quality and quantity of intracellular lipids, type of cells, their function and morphology. The intracellular milieu of cryopreserved cells and tissues remain less understood. The application of nanotechnology may help reveal and help advance our knowledge of the cryobiological principles involved in cryosurvival. At this moment the methods of cryopreservation that merit further investigation are vitrification and lyophilization. Vitrification is cheap if reagents are prepared in-house and the procedure can be performed rapidly. It has been successfully applied for gametes and embryos (of different stages of development), and reproductive cells/tissues, somatic cells and stem cells. However, vitrification is more demanding technically and requires operation and storage at sub-zero temperatures. On the other hand lyophilization deserves further investigation because it is a cheaper form of cryopreservation that may enable cryostorage at less demanding temperatures of 4°C and may even allow transport at ambient temperature. These possibilities are explored in this review.

scholarly journals Carboxylated Poly-l-Lysine as a Macromolecular Cryoprotective Agent Enables the Development of Defined and Xeno-Free Human Sperm Cryopreservation Reagents

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1435
Author(s):  
Hiroki Takeuchi ◽  
Mikiko Nishioka ◽  
Tadashi Maezawa ◽  
Yuko Kitano ◽  
Kento Terada-Yoshikawa ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Human Sperm ◽  
Allergic Reactions ◽  
Sperm Cryopreservation ◽  
Cryoprotective Agents ◽  
Long Term Storage ◽  
Term Storage

In human sperm cryopreservation, test yolk buffer and human serum albumin have been used as permeating macromolecular-weight cryoprotectants. In clinical reproductive medicine, human serum albumin is frequently used because of low risks of zoonoses and allergic reactions. However, the risk of allogeneic infectious diseases exists, and the supply may be unstable because human serum albumin is derived from human blood. Therefore, the development of xeno-free human sperm cryopreservative reagents that could overcome the aforementioned problems is warranted. We succeeded in developing a new xeno-free and defined sperm cryopreservation reagent containing glycerol, carboxylated poly-l-lysine, and raffinose. The cryopreservation reagent was not significantly different in terms of sperm motility, viability, and DNA fragmentation and was comparable in performance to a commercial cryopreservation reagent containing human serum albumin. Moreover, the addition of saccharides was essential for its long-term storage. These results may help elucidate the unknown function of macromolecular-weight permeating cryoprotective agents.

scholarly journals Isolation and Thermal Stabilization of Bacteriocin Nisin Derived from Whey for Antimicrobial Modifications of Polymers

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Pavlina Holcapkova ◽  
Zuzana Kolarova Raskova ◽  
Martina Hrabalikova ◽  
Alexandra Salakova ◽  
Jan Drbohlav ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Polyethylene Glycol ◽  
High Performance ◽  
Thermal Stabilization ◽  
Nisin Production ◽  
Long Term Stability ◽  
Long Term Storage ◽  
The Stability ◽  
Term Storage

This work describes novel alternative for extraction of bacteriocin nisin from a whey fermentation media and its stabilization by using polyethylene glycol as matrix with high practical applicability. This product was compared with commercially available nisin product stabilized by sodium chloride and nisin extracted and stabilized by using ammonium sulfate and polysorbate 80. The stability of samples was tested by means of long-term storage at −18, 4, 25, and 55°C up to 165 days. The nisin content in the samples was determined by high-performance liquid chromatography and electrophoresis. In addition, effect of whey fortification with lactose on nisin production and antibacterial activity studied against Staphylococcus aureus was tested. Results show that stabilization by polyethylene glycol provides enhanced nisin activity at 55°C after 14 days and long-term stability at 25°C with keeping antibacterial activity.

scholarly journals Improved lyophilization conditions for long-term storage of bacteriophages

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Prasanth Manohar ◽  
Nachimuthu Ramesh
Keyword(s):  
Antibiotic Resistance ◽  
Polyethylene Glycol ◽  
Phage Therapy ◽  
Pharmaceutical Products ◽  
Long Term Storage ◽  
Pharmaceutical Applications ◽  
The Stability ◽  
Term Storage

Abstract Phage therapy is one of the promising alternatives to combat the increasing problem of antibiotic resistance. Lyophilization is used for the preparation of pharmaceutical products to improve their stability in long-term storage. The aim of this study was to improve the stability of lyophilized bacteriophages using different excipients. Three lytic bacteriophages Escherichia phage ECP311, Klebsiella phage KPP235 and Enterobacter phage ELP140 were subjected to lyophilization using six different excipients: glucose, sucrose, gelatin, mannitol, polyethylene glycol and sorbitol. The lyophilized phages were stored at 4 °C and 37 °C and rehydrated using biological saline to test their viability at 5 months interval up to 20 months. The results showed that the use of sucrose, gelatin and their combination was beneficial in maintaining the viability of phages post-lyophilization. When lyophilized phages were stored at 4 °C, their viability was maintained up to 20 months, but at 37 °C there was a reduction in activity after 10 months. This is one of the few studies to report the lyophilization of phage cocktails to have viability for up to 10 months. Our study identified promising lyophilization excipients to effectively lyophilize bacteriophages for pharmaceutical applications and long-term storage.

Modelling of Moisture Movement in Wood during Outdoor Storage

2001 ◽  
Vol 6 (2) ◽  
pp. 3-14 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
I. Juodeikienė ◽  
A. Kajalavičius
Keyword(s):  
Experimental Data ◽  
Finite Difference ◽  
Climatic Conditions ◽  
Two Dimensional ◽  
Moisture Movement ◽  
Finite Difference Technique ◽  
Long Term Storage ◽  
Space Formulation ◽  
Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

Trehalose: a cryoprotectant that enhances recovery and preserves function of human pancreatic islets after long-term storage

Diabetes ◽  
1997 ◽  
Vol 46 (3) ◽  
pp. 519-523 ◽  
Author(s):  
G. M. Beattie ◽  
J. H. Crowe ◽  
A. D. Lopez ◽  
V. Cirulli ◽  
C. Ricordi ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Human Pancreatic Islets ◽  
Long Term Storage ◽  
Term Storage
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