Development of Cultivation Technology for the Escherichia Coli Recombinant Strain Producing Arginine Deiminase of Mycoplasma Hominis

The paper concentrated on the optimization of the recombinant strain BL21 (DE3)-PE7-Nit. The component of culture medium and the culture conditions were optimized. The optimized medium was: yeast extract 10 g/l, L-glutamate sodium 8 g/l, MgSO4.7H2O 0.7 g/l, Isopropyl-β-D-thiogalactopyranoside 0.3 mmol/L, potassium hydrogen phosphate 0.5 g / L, phosphate Potassium 0.5 g / L and the culture condition was: initial pH 7.0, inoculum 2%. The result showed that the activity of nitrilase prepared with these conditions increased by 130.37 % through optimization.

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Cloning and expression in Escherichia coli of the genes of the arginine deiminase system of Streptococcus sanguis NCTC 10904.

Infection and Immunity ◽

10.1128/iai.57.11.3540-3548.1989 ◽

1989 ◽

Vol 57 (11) ◽

pp. 3540-3548 ◽

Cited By ~ 25

Author(s):

R A Burne ◽

D T Parsons ◽

R E Marquis

Keyword(s):

Escherichia Coli ◽

Arginine Deiminase ◽

Cloning And Expression ◽

Streptococcus Sanguis ◽

Expression In Escherichia Coli

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High yield production of the latex clearing protein from Gordonia polyisoprenivorans VH2 in fed batch fermentations using a recombinant strain of Escherichia coli

Journal of Biotechnology ◽

10.1016/j.jbiotec.2019.12.013 ◽

2020 ◽

Vol 309 ◽

pp. 92-99

Author(s):

Anna-Lena Altenhoff ◽

Sven Thierbach ◽

Alexander Steinbüchel

Keyword(s):

Escherichia Coli ◽

Recombinant Strain ◽

High Yield ◽

Fed Batch ◽

Yield Production ◽

High Yield Production ◽

Gordonia Polyisoprenivorans

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Cloning of the nptII gene of Escherichia coli and construction of a recombinant strain harboring functional recA and nptII antibiotic resistance

Genetics and Molecular Research ◽

10.4238/vol10-3gmr1334 ◽

2011 ◽

Vol 10 (3) ◽

pp. 1445-1454 ◽

Cited By ~ 5

Author(s):

S. Ghanem

Keyword(s):

Escherichia Coli ◽

Antibiotic Resistance ◽

Recombinant Strain ◽

Nptii Gene

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Expression, purification, and characterization of arginine deiminase from Lactococcus lactis ssp. lactis ATCC 7962 in Escherichia coli BL21

Protein Expression and Purification ◽

10.1016/j.pep.2006.12.002 ◽

2007 ◽

Vol 53 (1) ◽

pp. 9-15 ◽

Cited By ~ 24

Author(s):

Jong-Eun Kim ◽

Do-Won Jeong ◽

Hyong Joo Lee

Keyword(s):

Escherichia Coli ◽

Lactococcus Lactis ◽

Arginine Deiminase ◽

Purification And Characterization ◽

Escherichia Coli Bl21

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Use of a Recombinant Strain of Mycobacterium avium Expressing β-Galactosidase To Evaluate the Activities of Antimycobacterial Agents inside Macrophages

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.45.1.356-358.2001 ◽

2001 ◽

Vol 45 (1) ◽

pp. 356-358 ◽

Cited By ~ 1

Author(s):

Giuseppantonio Maisetta ◽

Giovanna Batoni ◽

Manuela Pardini ◽

Antonella Boschi ◽

Daria Bottai ◽

...

Keyword(s):

Escherichia Coli ◽

Mycobacterium Avium ◽

Recombinant Strain ◽

Antimicrobial Agents ◽

Low Cost ◽

Rapid Screening ◽

Murine Macrophages ◽

Antimycobacterial Agents

ABSTRACT A reliable and low-cost method that enables rapid screening of the activity exerted by new antimicrobial agents on intracellularly growingMycobacterium avium has been developed. To this aim, a recombinant (lacZ) strain of M. aviumexpressing the Escherichia coli β-galactosidase gene was used to evaluate, in murine macrophages, the susceptibility of M. avium to common antimycobacterial agents. β-Galactosidase levels, measured in the presence of each of the antibiotics tested, were closely correlated with the number of CFU recovered from theM. avium lacZ strain-infected macrophages.

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Two New Point Mutations at A2062 Associated with Resistance to 16-Membered Macrolide Antibiotics in Mutant Strains ofMycoplasma hominis

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.45.10.2958-2960.2001 ◽

2001 ◽

Vol 45 (10) ◽

pp. 2958-2960 ◽

Cited By ~ 26

Author(s):

Pio Maria Furneri ◽

Giancarlo Rappazzo ◽

Maria Pia Musumarra ◽

Patrizia Di Pietro ◽

Lucrezia S. Catania ◽

...

Keyword(s):

Escherichia Coli ◽

Point Mutations ◽

Mycoplasma Hominis ◽

23S Rrna ◽

Macrolide Antibiotics ◽

Rrna Gene ◽

In Vitro Exposure ◽

Mutant Strains ◽

23S Rrna Gene

ABSTRACT We describe two mutants of Mycoplasma hominis PG-21 which show resistance to 16-membered macrolides but susceptibility to lincosamides, obtained by in vitro exposure to increasing doses of josamycin. The 23S rRNA gene showed that each had a mutation (A2062G and A2062T) corresponding to nucleotide 2062 in Escherichia coli, which was associated with the acquired phenotype.

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