Sufficient Blood Culture Volume

PEDIATRICS ◽  
2021 ◽  
Author(s):  
William A. Woods
Keyword(s):  
Blood Culture ◽  
Culture Volume

Optimizing blood culture practices in pediatric immunocompromised patients: evaluation of media types and blood culture volume

2003 ◽  
Vol 22 (6) ◽  
pp. 545-552 ◽  
Author(s):  
ADITYA H. GAUR ◽  
MARY ANNE GIANNINI ◽  
PATRICIA M. FLYNN ◽  
JAN W. BOUDREAUX ◽  
MARK A. MESTEMACHER ◽  
...  
Keyword(s):  
Blood Culture ◽  
Immunocompromised Patients ◽  
Culture Volume ◽  
Media Types

Effect of Clinical Variables on the Volume of Blood Collected for Blood Cultures in an Adult Patient Population

2017 ◽  
Vol 38 (12) ◽  
pp. 1493-1497 ◽  
Author(s):  
R. Logan Jones ◽  
Harlan R. Sayles ◽  
Paul D. Fey ◽  
Mark E. Rupp
Keyword(s):  
Blood Culture ◽  
Clinical Laboratory ◽  
Statistical Significance ◽  
Academic Medical Center ◽  
Blood Cultures ◽  
Charlson Comorbidity Index Score ◽  
Central Venous ◽  
Culture Volume ◽  
Clinical Variables ◽  
Volume Recovery

OBJECTIVETo identify clinical variables that influence blood culture volume recoveryDESIGNRetrospective chart review and linear model analysisSETTINGA 621-bed Academic Medical Center with a Clinical Laboratory that processes 20,000+ blood cultures annually and dedicated phlebotomy staff for venipuncturePATIENTSConsecutive patients requiring blood cultureMETHODSOver a 6-day period, blood volume was determined in 568 culture bottles from 128 unique adult patients, and clinical data from the time of phlebotomy were extracted from hospital electronic medical records. Conditional hierarchical linear models with random effects for patient and phlebotomy occasion were utilized to analyze correlations between values collected from the same patient and during the same phlebotomy occasion.RESULTSBlood samples obtained from a central venous catheter yielded, on average, 2.53 mL more blood (95% CI, 1.63–3.44 mL;P<.001) than those from peripheral venipuncture, and aerobic bottles contained 0.38 mL more blood (95% CI, 0.1–0.67 mL;P=.009) than the anaerobic bottles. The remaining clinical variables (eg, hospital department, patient age, body mass index, gender, mean arterial pressure, concomitant systemic antibiotic use, and Charlson comorbidity index score) failed to reach statistical significance (P<.05) in relation to volume.CONCLUSIONSBlood cultures obtained from central venous catheters contain significantly greater volume than those obtained via peripheral venipuncture. These data highlight the clinically significant issue of low culture volume recovery, indicate that diagnostic and prognostic tools that rely on volume-dependent phenomena (ie, time to positivity) may require further validation under usual clinical practice circumstances, and suggest goals for future institutional performance improvement.Infect Control Hosp Epidemiol2017;38:1493–1497

The practice of blood volume submitted for culture in a neonatal intensive care unit

2020 ◽  
Vol 105 (6) ◽  
pp. 600-604
Author(s):  
Moni Pankhuri Singh ◽  
Kiran Kumar Balegar V ◽  
Rajeshwar Reddy Angiti
Keyword(s):  
Intensive Care ◽  
Blood Culture ◽  
Blood Volume ◽  
Neonatal Intensive Care ◽  
Newborn Infants ◽  
Sample Volume ◽  
Culture Bottle ◽  
Culture Volume ◽  
Mortality And Morbidity ◽  
The Impact

BackgroundNeonatal sepsis is the leading cause of mortality and morbidity in neonatal intensive care units. The volume of blood taken for culture remains one of the most important factors in isolating microorganisms.ObjectivesTo evaluate the impact of the intervention on the blood volume submitted for culture and to identify factors influencing the volume as determined by the phlebotomist.MethodsBlood culture volume was determined by weighing the culture bottle before and immediately after blood inoculation. A 3-month preintervention audit revealed that in 126/130 samples (96.9%), the volume of blood submitted was suboptimal. Multiple intervention measures were instituted, and volume was monitored over the next 9 months.Results637 blood culture samples were included in the study, 130 were in preintervention and 507 were in postintervention epochs. Following the intervention, suboptimal volume samples reduced from 96.9% (126/130 samples) to 25% (126/507 samples), p<0.0001 and the median (IQR) sample volume improved from 0.36 (0.23) ml to 0.9 (0.27) ml, p<0.0001. Poor blood flow was identified as the most common reason for an inadequate sample.ConclusionThe study underscores the role of educational intervention in improving the blood culture volume in newborn infants. Poor backflow from the cannula is an important cause of inadequate volume collection.

ANTIMICROBIAL SUSCEPTIBILITY IN CULTURE POSITIVE ENTERIC FEVER

2019 ◽  
Vol 3 (7) ◽  
Author(s):  
Dr. Manish Kulshrestha ◽  
Dr. Anjali Kulshrestha
Keyword(s):  
Bone Marrow ◽  
Blood Culture ◽  
Nalidixic Acid ◽  
Antimicrobial Susceptibility ◽  
Salmonella Enterica ◽  
Enteric Fever ◽  
Multidrug Resistant ◽  
Bone Marrow Culture ◽  
Diffusion Method ◽  
Culture Positive

INTRODUCTION: Enteric fever includes typhoid and paratyphoid fever. Peak incidence is seen in children 5–15 years of age; but in regions where the disease is highly endemic, as in India, children younger than 5 years of age may have the highest infection rates. There are about 22 million new typhoid cases occur each year. Young children in poor, resource limited areas, who make up the majority of the new cases and there is a mortality figures of 215,000 deaths annually. A sharp decline in the rates of complications and mortality due to typhoid fever is observed as a result of introduction of effective antibiotic therapy since 1950s. MDR-ST became endemic in many areas of Asia, including India soon after multidrug-resistant strains of Salmonella enterica serotype typhi (MDR-ST) that were resistant to all the three first-line drugs then in use, namely chloramphenicol, amoxycillin and co-trimoxazole emerged in early 1990s. MATERIAL AND METHODS: Only blood culture or bone marrow culture positive cases were included. The patients with culture isolated enteric fever were included in the study. Antimicrobial susceptibility testing was carried out by disk diffusion method using antibiotic discs. The analysis of the antimicrobial susceptibility was carried out as per CLSI interpretative guidelines. RESULTS: A total of 82 culture positive cases were included in the present study. 80 culture isolates were from blood culture and 2 from the bone marrow culture. Salmonella entericasubspecies enterica serovartyphi (S typhi) was isolated from 67 (81.70%) patients while Salmonella enterica subspecies entericaserovarparatyphi (S paratyphi A) was isolated from 13 (15.85%) cases and 2 (2.44%) were Salmonella enterica subspecies entericaserovarschottmuelleri (S paratyphi B). Of the 82 cases 65(79.3%) isolates were resistant to ciprofloxacin, 17 (20.7%) were resistant to nalidixic acid, one (1.2%) case each was resistant to Cefotaxime and ceftriaxone, 2 (2.4%) were resistant to chloramphenicol, 10 (12.2%) were resistant and to cotrimoxazole 3 (3.7%) were resistant. CONCLUSION: In a culture positive cases 65(79.3%) isolates were resistant to ciprofloxacin and 17 (20.7%) were resistant to nalidixic acid. Multidrug resistant isolates were 65(79.3%).

Inefficiency in 6-Set Requests for Blood Culture Analysis of Cancer Patients with the Central Venous Catheter

2013 ◽  
Vol 3 (3) ◽  
pp. 155
Author(s):  
Dong-Hyun Lee ◽  
Eun-ha Koh ◽  
Sunjoo Kim ◽  
In-Gyu Bae ◽  
Hoon-gu Kim ◽  
...  
Keyword(s):  
Blood Culture ◽  
Central Venous Catheter ◽  
Cancer Patients ◽  
Venous Catheter ◽  
Central Venous
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