scholarly journals The effect of pre-sowing seed treatment on seedlings growth rate and their excretory activity

2020 ◽  
Vol 11 (1) ◽  
pp. 60-66
Author(s):  
A. I. Bozhkov ◽  
M. K. Kovalova ◽  
Z. A. Azeez ◽  
А. V. Goltvjansky

The importance of studying pre-sowing seed treatment lies in the possibility of regulating the rate of seed germination, the intensity of their growth and obtaining root exudates in biotechnology. The effect of three pre-sowing treatment methods was examined (control – washing with running water; the first method – washing with 0.05% sodium permanganate solution; the second method – 30 se­conds in 70% ethyl alcohol (C2H5OH) and 30 minutes in 5% sodium hypochlorite (NaOCl); the third method – 5 minutes in 70% C2H5OH and 40 minutes in 5% NaOCl) on the growth rate, germination rate, excretion rate of seeds of wheat and peas and composition (of protein, carbohydrate, amino acid content) of root exudates from the first to the third day of growth in order to obtain root exudates. It was revealed that the same pre-sowing treatment of wheat and pea seeds has a different effect on the rate and variability of seedling growth from the first to the third day, as well as on the qualitative and quantitative composition of root exudates. It was shown that pre-sowing treatment of wheat and pea seeds for 5 minutes with 70% ethanol followed by treatment with sodium hypochlorite (a “hard” treatment method) accelerates seedling growth and seed germination. This method of treatment reduces the intensity of excretion of root exudates and composition in wheat, but it increases the intensity of excretion in peas. The discovered effects can be explained by hormesis. Additionally, the third method of pre-sowing seed treatment can be used in root technologies for obtaining root exudates.

2005 ◽  
Vol 23 (1) ◽  
pp. 4-8 ◽  
Author(s):  
Tyler L. Burgess ◽  
Frank A. Blazich ◽  
David L. Nash ◽  
Betsy Randall-Schadel

Abstract Seeds of southern seaoats (Uniola paniculata L.) were removed from storage at 4C (39F) and treated with the following selected surface disinfestants, fungicides, or combinations of these chemicals: nontreated (control), 1.3% sodium hypochlorite [NaOCl (chlorine bleach)], 2.6% sodium hypochlorite, RTU® (12.6% thiram + 0.34% thiabendazole), RTU®-PCNB (24% pentachloronitrobenzene), 1.3% sodium hypochlorite and RTU®, 2.6% sodium hypochlorite and RTU®, 1.3% sodium hypochlorite and RTU®-PCNB, or 2.6% sodium hypochlorite and RTU®-PCNB. Following treatment, seeds were germinated at an 8/16 hr thermoperiod of 35/20C (95/68F). The seed treatments and germination thermoperiod utilized were based on three preliminary trials that investigated the influence of selected surface disinfestants, fungicides, and temperature on seed germination of the species. Germination was recorded every 3 days for 30 days. Seed treatment was highly significant (P = 0.0001) for both total percentage germination and total percentage of decayed seeds. Germination of nontreated seeds was 45%, and four treatments resulted in germination > 80% [RTU®-PCNB (81%), 2.6% sodium hypochlorite and RTU® (83%), 1.3% sodium hypochlorite and RTU® (87%), and 1.3% sodium hypochlorite and RTU®-PCNB (89%)]. A subsequent experiment investigated the effects of the aforementioned treatments with the exception of 1.3% sodium hypochlorite and RTU®, both used alone, on initial seedling growth of the species. Following treatment, seeds were sown in containers filled with a peat-based medium and the containers placed in a growth chamber maintained at an 8/16 hr thermoperiod of 35/20C (95/68F) with long day conditions. Emergence data were recorded every 3 days for 45 days. After 45 days, the study was terminated and additional data recorded to include plant height (height of main stem), leaf number, length and width of the two longest leaves, and top and root dry weights. Surface disinfestant, fungicide, and combination treatments were highly significant (P = 0.0004). Percentage emergence of nontreated seeds was 35% and five of the seven treatments resulted in emergence ≥ 75% [2.6% sodium hypochlorite (75%), 1.3% sodium hypochlorite and RTU® (75%), 1.3% sodium hypochlorite and RTU®-PCNB (76%), 2.6% sodium hypochlorite and RTU®-PCNB (81%), and 2.6% sodium hypochlorite and RTU® (83%)] with negligible effects on seedling growth. There were significant treatment differences regarding some of the variables used to evaluate seedling growth. In most cases these differences were due to seedlings from nontreated seeds having lower values for each measured variable than values for the same variables from treated seeds. Results of both experiments demonstrate the potential value of chemical seed treatment during production of seedling transplants of U. paniculata.


Author(s):  
Amit Kumar Tiwari ◽  
Prawal P.S. Verma ◽  
Dipender Kumar ◽  
Sonveer Singh ◽  
Rakesh Kumar ◽  
...  

Background: Sarpagandha is an indigenous medicinal herb of Indian continent. It has many medicinal properties. Due to increasing demand of Sarpagandha, its exploitation is increasing continuously but for lack of cultivation, this plant has been listed in endangered category. Commercially, it is propagated through seeds but the main barrier of seed propagation is its irregular and low germination. Hence, the current study was undertaken to study the effect of growing media and seed treatment methods on seed germination and seedling growth of Sarpagandha to eliminate the inhibitory effect of the hard stony endocarp.Methods: Seed germination and seedling growth experiments of Sarpagandha were carried out with following different seed treatments, T1 (Control), T2 (Water soaking for 24 hours), T3 (water soaking for 48 hours), T4 (Water soaking for 24 hours + cotton cloth wrapping for 24 hours), T5 (GA3 200 ppm), T6 (FYM treatment for 24 hours), T7 (FYM treatment for 48 hours), T8 (Hot water treatment) and T9 (Cow dung treatment for 24 hours). Experiment was laid out in CRD. The data recorded on the various parameters were analyzed at 5% level of significance by using ANOVA.Result: Results indicated highest germination percentage (51), lowest mortality percent (49), minimum days for initiation of germination (23), minimum days taken for germination completion (33), highest speed of germination (3.77), maximum vigour index (1441.44), highest root (11.27 cm) and highest shoot length (17 cm) in T5 (GA3 200 ppm) followed by T3 (water soaking for 48 hours). Indicating, T5 (GA3 200 ppm) to be best seed treatment for higher germination and subsequent seedling growth of Sarpagandha (Rauvolfia serpentine L.).


1968 ◽  
Vol 48 (3) ◽  
pp. 281-286 ◽  
Author(s):  
G. A. Kemp

The effects of low temperatures on several growth phases of the tomato were studied. The tests, each conducted over a 2-week period, included seed germination at 8.5 °C, rate of seedling growth at a night temperature of 10 °C, root and top growth of plants in soil at 15 °C, and fruit set at a night temperature of 4.5 °C.Varietal response to growth rate at low temperatures differed in all phases studied, and varieties that performed well in some phases did not always perform well in others. Of the varieties studied, six grew well under most of the low-temperature conditions. These were: Earlinorth, Bonita, Azerbidzivisky, P.I. 205040, P.I. 280597, and Cold Set.


2020 ◽  
Vol 16 (2) ◽  
pp. 270-276
Author(s):  
Basavaraj L. Tamadaddi ◽  
G. Prabhuling

An investigation was undertaken on two medicinally important evergreen tree species of Western Ghats namely, Elaeocarpus munronii and Canarium striuctum to improve seed germination and enhance seedling growth in nursery. The seeds collected from two randomly chosen trees in each species were used for the study. In general seed germination was very poor in both the species. The maximum germination of 4.66 per cent was observed in Elaeocarpus munronii and it was 13.33 per cent in Canarium striuctum. Though big and medium size seeds recorded higher germination per cent than small size seeds in both the species studied, the seed size effect on germination was found to be non-significant. The results of the study revealed that seed size did influence seedling growth in both the species. Seeds of bigger size produced seedlings with higher values of shoot length, root length and collar diameter but their effect on dry matter accumulation was not significant. The effect of seed treatments on seedling growth was not noticed in Elaeocarpus munronii while seed treatment with sulphuric acid in Canarium striuctum showed slight but significant increase in shoot length, however, other growth parameters remained unaffected. The effects of seed size as well as of seed treatment on germination and seedling growth was found to be similar in both the trees species studied.


2019 ◽  
Vol 48 (2) ◽  
pp. 223-229 ◽  
Author(s):  
Kürşat Çavuşoğlu ◽  
Fadime Doğu ◽  
Dilek Çavuşoğlu

The effects of sodium hypochlorite (NaClO) on the seed germination, seedling growth (radicle length, radicle number and fresh weight), mitotic activity and chromosomal aberrations of Allium cepa L. germinated under salt stress were studied. Salt stress considerably inhibited the seed germination and seedling growth of A. cepa. Furthermore, it markedly reduced the mitotic index in root tip meristems of the seeds and increased the number of chromosomal aberrations. Whereas, the detrimental effects of salt on the seed germination, seedling growth, mitotic activitiy and chromosomal aberrations were dramatically alleviated in varying degrees by NaClO application.


2013 ◽  
Vol 100 (2) ◽  
pp. 179-184 ◽  
Author(s):  
Audrius Radzevičius ◽  
Sandra Sakalauskienė ◽  
Mindaugas Dagys ◽  
Rimantas Simniškis ◽  
Rasa Karklelienė ◽  
...  

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