scholarly journals ІНТЕНСИВНІСТЬ ПРОЦЕСІВ ПОЛ ТА АКТИВНІСТЬ СИСТЕМИ АНТИОКСИДАНТНОГО ЗАХИСТУ У КРОВІ КОРОПІВ ЗА ДІЇ РІЗНОГО РІВНЯ ВІТАМІНУ Е І СЕЛЕНУ В РАЦІОНІ

2016 ◽  
Vol 18 (3(71)) ◽  
pp. 201-204
Author(s):  
S.V. Yurchak ◽  
O.V. Derenj ◽  
O.I. Vishchur ◽  
Yu.M. Zabytivskyi

The article consist data about effect of different levels of vitamin E and selenium in the diet of carps during their growing and also informationabout the influence on processes of lipid peroxidation and activity of antioxidant protection in their body.The experiment conducted in three experimental ponds. After wintering there were placed four ears mature females and males carp, six individuals in each group. Supplements of vitamin E administered at a rate of 25 mg/kg and drug «Sel–Plex» the rate of selenium – 0.3 mg/kg were added to further basic diet(BD) of female and male carp first experimental group (EG1). The second experimental group (EG2) received (BD) and vitamin E supplements in an amount of 75 mg / kg, and just as in EG1 – selenium – 0.3 mg/kg of feed.The control group received fish feed without additives vitamins and minerals.Feeding lasted for 30 days, due to the physiological needs of the fish feed. After spawning in fish of research and control group swere taken blood samples for biochemical research.The study led to a dose–dependent decrease (p < 0.01 – 0.001) content of TBA–active products and hydroperoxidase of lipids, but did not significantly effect onsuperoxide dismutase and glutathione peroxidase activity of blood So, the reduction of lipid peroxidation products in the carp’s blood of experimental groups probably was caused by the growth of non–enzymatic level, which is associated with fat–soluble vitamins. 

2017 ◽  
Vol 6 ◽  
Author(s):  
Analía Lorena Risso ◽  
Francisco J. Pellegrino ◽  
Yanina Corrada ◽  
Mónica Marmunti ◽  
Mariana Gavazza ◽  
...  

AbstractThe objective was to evaluate the effects of dietary fish oil (FO) and vitamin E (VE) supplementation on sperm sensitivity to lipid peroxidation (LP) in dogs. Using an incomplete replicate 3 × 3 Latin square design, five dogs were allocated into three groups. One of the squares was incomplete and had two dogs that were used with three treatments. The dogs were assigned to three different treatments, fed a control diet of balanced commercial food (control group; CG), control diet supplemented with 54 mg FO/kg body weight0·75per d (FO group; FG) and FO plus 400 mg VE per d (FO and VE group; FEG) for 60 d. Semen samples were collected on days 0 and 60 and divided into two halves, peroxidised and control, with or without ascorbate–Fe2+, respectively. LP was measured in both halves by chemiluminescence as counts per min/mg protein. Fatty acid profile was determined by GC. Data were analysed using the mixed procedure (SAS). On day 0, LP increased in all groups in the peroxidised samples (P< 0·05). However, on day 60 LP decreased in peroxidised samples of both the FG and FEG (P< 0·05), but there were no differences between the FG and FEG (P> 0·1). Additionally, on day 60 totaln-3 was higher in the FG and FEG compared with the CG (P< 0·05). Supplementation with FO alone or together with VE decreased LP in peroxidised samples. These results could indicate a protective effect ofn-3 on sperm. More studies are needed to understand the mechanism whereby FO and/or FO plus VE decrease LP in dogs’ sperm.


2009 ◽  
Vol 52 (3) ◽  
pp. 309-320
Author(s):  
M. Gabryszuk ◽  
K. Horbańczuk ◽  
J. Klewiec

Abstract. The experiment was conducted on 80 Polish Merino ewes, their 82 lambs and 40 fattening rams. Merino ewes were divided into two groups: experimental (E) and control (C), each composed of 40 animals. Four weeks before lambing, 1 day, 3 weeks and 6 weeks after lambing 40 ewes of the experimental (E) group received intramuscular injection of 5 ml 0.1 % Na2SeO4 (selenium [Se]: 2.09 mg), 10 ml 10 % ZnSO4 (Zinc [Zn]: 227 mg) and 250 mg vitamin E (α-tocopherol), and control group (C) – no received. From birth all the lambs were maintained with their dams and then weaned at the age of 8 weeks, placed in individual straw-bedded pens and fattened individually with a granulated concentrate mixture until reaching a body weight about 32 kg. During fattening 20 lambs from experimental group (E) was given per os 1 ml 0.1 % Na2SeO4, 3 ml 10 % ZnSO4 and 60 mg vitamin E (α-tocopherol) daily, and control group (C) – no administered. The levels of Se, Zn and vitamin E in the blood plasma of ewes and ram- lambs were within the reference values. The lambs born by the treated ewes (E) experienced much lower body live weight at birth (P≤0.05), body weight on day 28 and 56 (P≤0.01), compared to lambs born from group (C). Also the daily live weight gain from birth to 4 week and from birth to 8 week were significantly lower (P≤0.05) in lambs born by the treated ewes. Se, Zn and vitamin E treated rams contained less fat in leg (P≤0.05), less fat thickness over ribs (P≤0.01) and less fat thickness over loin-eye than non treated rams.


Author(s):  
Svetlana Vladimirovna Kotelnikova ◽  
Andrey Vyacheslavovich Kotelnikov ◽  
Alexander Nickolaevich Nevalennyy ◽  
Sergey Vladimirovich Ponomarev ◽  
Yulia Mikhailovna Shirina

The article studies the effect of addition into the feed of Sapropel extract (ES-2 preparation) on the intensity of lipid peroxidation in the liver and gills of hybrid tilapia ( Oreochromis spp. ), as well as on the morphofunctional state of its liver. Sapropel extract caused a decrease in the content of TBA-reactants in the tissues of tilapia liver by 17% compared to the control group. In gills the bioadditive resulted in the increased content of peroxide products by 24%. The introduction of ES-2 in fish feed resulted in reduction of spontaneous and ascorbate-dependent lipid peroxidation rate in the liver by 18%. In the gills of fish, under the influence of Sapropel, the rate of spontaneous lipid peroxidation increased by 27%, the rate of ascorbate-dependent lipid peroxidation - by 23%. The change in the intensity of peroxide processes under the influence of the fodder additive in fish organs is tissue-specific: antioxidant effect was recorded in the liver, prooxidant effect was observed in the gills. The introduction of the Sapropel extract does not lead to a change in the volume of liver nuclei in the test groups of tilapia, while the average cell volume in the experimental group was 37% lower than in the control group. The decrease in cell volume led to the increase in the nuclear-cytoplasmic ratio by 1.9 times in the experimental group compared to the control group. Hepatocyte cytoplasm volume decrease and nuclear-cytoplasmic ratio increase due to addition of ES-2 preparation into productive feed of hybrid tilapia would indicate a rise of functional activity of liver cells.


Author(s):  
L. M. Zlamanyuk

<p>We studied the influence of a different level of a calcium and phosphorus in mixed fodders on morphological and biochemical indexes of a blood of Japanese quail. We selected 240 quails, which were divided into 6 equal groups - control and 5 experimental. Experimental quails were contained in six-fodders cell battery, each cell was 60x40x20 cm and contained of 20 birds (15 females and 5 males). At the beginning of the experiment the experimental quails had similar body weight indicators that were the same in the end of our research. When we fed quails with different levels of calcium and phosphorus wew registered that calcium, magnesium, total protein and alkaline phosphatase activity in their blood was within physiological norm. We identified no significant differences in these indicators. It should be noted that the highest content of phosphorus in the quail blood was observed in female birds of 6th experimental group, which exceeded by 23.1% the values in quails of control group with calcium content of 4.0% and phosphorus of 1.4% (P &lt;0.05). The introduction of different level of a calcium and phosphorus were not significantly affect the blood erythrocytes, leukocytes, and hemoglobin. The ratio of segmented rods, eosinophils, lymphocytes and monocytes in the blood of experimental birds was within the physiological norm of fluctutations. In content, there was a slight deviation (within 1%) was not statistically significant in the blood of the bird experimental and control groups, eosinophils and monocytes. The lymphocyte content was below 8.3% in the blood of all experimental bird groups. It is revealed that a level of a calcium of 4.0 % and phosphorus of 0.8, 1,1, and 1,4 % in rations of quails, promotes rising of a calcium level in skeleton of female quails and does not influence significantly the morphological and biochemical blood indices.</p>


Author(s):  
Eka Rejeki Maha ◽  
Berlin Sibarani

This study was aimed at finding out The Effect of Applying POSSE (Predict-Organize-Search-Summarize-Evaluate) Strategy on the Students’ Reading Comprehension. This study used the experimental design. The population of this study was the students of SMA Negeri 2 Medan. There were sixty students taken as the sample of the research. This study was conducted with two randomized groups namely experimental and control group. The experimental group was taught by applying POSSE strategy while control group was taught without applying POSSE strategy. The instrument of collecting the data was multiple choice test which consists of 40 items. To obtain the reliability of the test, the researcher used Kuder-Richardson (KR-21). The calculation shows the reliability was 0.81(high). The data were analyzed by using t-test. The calculation shows that t-observed (4.76) is higher than t-table (2.00) at the level of significance (α) 0.05 with the degree of freedom (df) 58. Therefore, the null hypothesis (Ho) is rejected and alternative hypothesis (Ha) is accepted. It means that there was a significant effect of applying POSSE strategy on the students’ reading comprehension. Keywords: POSSE Strategy, Reading Comprehension.


Author(s):  
Haryato Siregar ◽  
Elia Masa Ginting

This study was aimed at finding out the effect of applying Learning Together Method on the students’ reading comprehension. This study used the experimental design. The population of this study was the students of SMP Negeri 1 Muara. There were sixty students of eighth class as the sample of the research. This study was conducted with two randomized groups namely experimental and control group. The experimental group was taught by applying Learning Together Method while control group was taught without applying Learning Together Method. The instrument of collecting the data was multiple choice test which consists of 20 items. To obtain the reliability of the test, the researcher used Kuder-Richardson (KR-21) formula. The calculation shows the reliability was 0.82(high). The data were analyzed by using t-test. The calculation shows that t-observed (4.9) is higher than t-table (2.00) at the level of significance (α) 0.05 with the degree of freedom (df) 58. Therefore, the null hypothesis (Ho) is rejected and alternative hypothesis (Ha) is accepted. It means that teaching reading comprehension by applying Learning Together Method significantly affect reading comprehension. Keywords: Learning Together Method, Reading Comprehension


Author(s):  
Hasanul Arifin Zul And Masitowarni Siregar

This thesis is focused on the investigation of the effect of applying animal cartoon pictures on students’ achievement in writing narrative text. This study aims to find whether applying animal cartoon pictures significantly affect the students’ writing achievement or not. The data in this study were obtained by administering a written test. The population was the 2015/2016 first year (grade XI) of SMA Swasta Nusantara Lubuk Pakam and 66 students were taken as the sample by using random sampling. The sample was divided into two groups, experimental and control groups. The experimental group was taught by applying animal cartoon pictures while the control group without animal cartoon pictures (x = lecturing). The data were taken the scores from the pre-test and post-test to both experimental and control groups. These data were analyzed by using t-test. The result of computing the t-test obviously showed that t-observed is higher than t-table (5.21 >1,67) with the degree of freedom 64 (df =N-2) at the level significance 0,05 one tail test. It showed that the application of animal cartoon pictures significantly affected the students of SMA Swasta Nusantara Lubuk Pakam achievement in writing narrative text.


Author(s):  
Sri Agus Murniasih And I Wayan Dirgeyasa

This study was conducted to know the effect of applying Peer Review Strategy on the students’ achievement in writing descriptive paragraph. It was conducted by using experimental research method. This research design was conducted pre-test, post test in experimental and control group. The population of this research was the eight (VIII) grade students of SMP IT IQRA’ Medan. The sample of this research was taken by lottery technique. They were in class VIII-1 by experimental group and the class VIII-2 by control group. The experimental group was taught by using Peer Review Strategy while the control group was given no treatment. The instrument used to collect the data was writing essay test. The data were analyzed by using t-test formula. The result showed that the value of t-observed was higher than the value of t-table. (3,55 > 2,05 (a = 0.05)) with the degree of freedom (df) = 28. It means that there was a significant effect of applying Peer Review Strategy on the students’ achievement in writing descriptive paragraph. So, the alternative hypothesis (Ha) was accepted.


Author(s):  
Meryanti Napitupulu And Anni Holila Pulungan

This study was conducted as an attempt to discover the effect of applying Demonstration Method on students’ achievement in speaking skill. It was an experimental research. The subject was students of Grade XII, Vocational High School (Sekolah Menengah Kejuruan: SMK), which consisted of 79 students. The research was divided into two groups: experimental and control groups. The instrument used to collect the data was speaking test. To obtain the reliability of the test, the writer applied Kuder Richardson 21 formula. The result of the reliability was 0.7, and it was found that the test was reliable. The data were analyzed by using t-test formula. The analysis showed that the scores of the students in the experimental group were significantly higher than the scores of the students in the control group at the level of significant m = 0.05 with the degree of freedom (df) 77, t-observed value 8.9 > t-table value 1.99. The findings indicate that using Demonstration Method significantly affected the students’ achievement in speaking skill. So, English teachers are suggested to use Demonstration Method in order to improve students’ achievement in speaking skill.


Author(s):  
Veronika N. And Lince Sihombing

This study focused on finding out the effect of applying RAP (Read, Ask,Paraphrase) Strategy on students’ reading comprehension in reading analyticalexposition text. This study was conducted in experimental design. The populationof this research was the second year of students in SMA Swasta Katolik BudiMurni 2 Medan. There were seventy students of the second year senior highschool students as the sample of the research. This study was conducted with tworandomized groups namely Experimental Group and Control Group. Theexperimental group was taught by applying RAP Strategy, while the control groupwas taught by applying conventional strategy. The instrument of collecting thedata was multiple choice tests which consisted of 25 items. The data wereanalyzed by using t-test formula. The calculation shown that t-observed (5.36)was higher than t-table (1.994) at the level of significance (α) 0.05 with the degreeof freedom (df) 68. It means that there was a significant effect of applying RAPStrategy on students’ reading comprehension in reading analytical exposition text.


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