scholarly journals Peculiarities of vascular endothelial growth factor of oral cavity in atopic condition VEGF of oral cavity in atopic condition

2021 ◽  
Author(s):  
Nazaryan Rozana ◽  
Kryvenko Liudmyla ◽  
Gargin Vitaliy
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Oral Cavity ◽  
Oral Mucosa ◽  
Inflammatory Cells ◽  
Atopic Disease ◽  
Vascular Endothelial ◽  
Atopic Condition ◽  
Expression Activity ◽  
Endothelial Growth Factor

AbstractBackground and aimsVascular endothelial growth factor (VEGF) is regarded as a potent stimulating factor for angiogenesis and vascular permeability and probably is connected with an inflammatory reaction. Our study aimed to determine the effect of VEGF in the inflammatory process in the oral mucosa of experimental animals in the modulation of atopic disease.Materials and methodsAtopic condition was simulated by the ovalbumin model. Obtained specimens of oral mucosa were examined histologically; immunohistochemistry was performed with detection VEGF, CD23, CD20.ResultsMost pronounced changes with twice increased expression activity of VEGF has been detected in the affected areas of the lamina propria and were associated with perivascular inflammatory microinfiltration, but unexpected expression in the epithelial layer has been revealed surround of intraepithelial inflammatory cells mainly. Pronounced correlations have been detected as VEGF and CD23 (r = 0.91), VEGF and CD20 (r = 0.87), CD23 and CD20 (r = 0.89).Discussiondescribed the changes in the tissues of the oral mucosa could be served as a basis for the development of preventive measures in patients with atopic diseases.discussionConclusionsActivation of VEGF is connected with accumulation of inflammatory infiltrate represented by B-lymphocytes, activated macrophages, eosinophils with a correlation in atopic process.

HIF-1 expression in healing wounds: HIF-1α induction in primary inflammatory cells by TNF-α

2001 ◽  
Vol 281 (6) ◽  
pp. C1971-C1977 ◽  
Author(s):  
Jorge E. Albina ◽  
Balduino Mastrofrancesco ◽  
Joseph A. Vessella ◽  
Claudine A. Louis ◽  
William L. Henry ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Hypoxia Inducible Factor ◽  
Inflammatory Cells ◽  
Raw 264.7 Cells ◽  
Vascular Endothelial ◽  
Oxygen Intermediates ◽  
Tnf Α ◽  
Endothelial Growth Factor

The expression of the hypoxia-responsive transcription factor hypoxia-inducible factor (HIF)-1 during acute inflammation was investigated in experimental wounds. HIF-1α mRNA was maximally expressed in wound cells 6 h after injury. HIF-1α protein was detectable in wound cells 1 and 5 days after injury. Cells from 1-day-old wounds were not hypoxic, as determined by lack of pimonidazole hydrochloride adduct formation. Tumor necrosis factor (TNF)-α, but not interleukin-1β, increased the HIF-1α protein content of cells isolated 1 and 5 days after injury, and also of glycogen-elicited peritoneal cells, but not HIF-1α mRNA. HIF-1α did not accumulate in TNF-α-treated HeLa, NIH/3T3, NR8383, or RAW 264.7 cells. Nitric oxide from S-nitrosoglutathione did not induce HIF-1α accumulation or modulate the response to TNF-α. TNF-α did not increase oxygen consumption or result in the production of reactive oxygen intermediates by day 1 wound cells. Vascular endothelial growth factor mRNA in wound cells peaked 24 h after wounding. HIF-1 expression in early wounds may contribute to the regulation of inducible nitric oxide synthase and vascular endothelial growth factor, two HIF-1-responsive genes intimately related to the process of repair.

scholarly journals Elevated Vascular Endothelial Growth Factor (VEGF) levels in the blood serum of dogs with malignant neoplasms of the oral cavity

2014 ◽  
Vol 62 (3) ◽  
pp. 362-371 ◽  
Author(s):  
Aleksandra Sobczyńska-Rak ◽  
Izabela Polkowska ◽  
Piotr Silmanowicz
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Oral Cavity ◽  
Blood Serum ◽  
Control Group ◽  
Malignant Neoplasms ◽  
Vascular Endothelial ◽  
Neoplastic Tissue ◽  
Tissue Samples ◽  
Endothelial Growth Factor

Angiogenesis plays an essential role in the development of a neoplastic tumour by conditioning both its growth and the formation of metastases. The induction of blood vessel growth occurs under the influence of proangiogenic factors, among which Vascular Endothelial Growth Factor (VEGF) seems to be the most important. The aim of this research was to study the level of VEGF measured by ELISA in the serum of dogs with neoplasms of the oral cavity. The study material comprised samples of neoplastic tissue from 17 operated dogs and the serum of the examined animals as well as of dogs from the control group. The tissue samples were taken from dogs of different breeds, aged 6–14 years. The tumour type was determined in accordance with the applicable WHO classification. Blood samples taken from sick dogs and from animals of the control group were centrifuged, and immunoenzymatic labelling of VEGF was performed in the obtained serum using ELISA and R&D system reagents (Quantikine Canine VEGF). All stages of VEGF labelling were performed according to the recommendation of the test manufacturer. The median of VEGF in the serum of the dogs with neoplasms of the oral cavity was 40.64 pg/mL. The lowest value of 14.26 pg/mL was observed in the case of fibrosarcoma, and the highest value of 99.19 pg/mL in the case of squamous cell carcinoma. The VEGF median in the control group amounted to 11.14 pg/mL whereas the VEGF value in the groups of animals diagnosed with benign tumours ranged between 2.30 and 19.74 pg/mL. Elevated VEGF in the blood serum, in comparison with the benign tumour group and the control group, was observed in all examined neoplasms of the oral cavity. It was suggested that overexpression of VEGF can have a prognostic value and is useful in the early detection of neoplasms.

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