Serological evidence for Babesia canis infection of horses and an endemic focus of B. caballi in Hungary

2007 ◽  
Vol 55 (4) ◽  
pp. 491-500 ◽  
Author(s):  
S. Hornok ◽  
Renate Edelhofer ◽  
G. Földvári ◽  
Anja Joachim ◽  
R. Farkas

In order to evaluate the seroconversion of horses to Babesia caballi and B. canis in Hungary, blood samples were collected from 371 animals on 23 different locations of the country. The presence of antibodies to B. caballi was screened with a competitive ELISA. All 29 positive samples came from one region (the Hortobágy). The prevalence of infection did not show correlation with sexes, and reached 100% in the age group of 2–5 years. Babesia canis -specific antibodies were demonstrated by IFAT in 6.74% of animals kept in 7 regions. The titres were low or medium level (1:40 to 1:160), indicating that the horses had previously been exposed to this piroplasm, but their infection must have been limited. The highest seropositivity rate was observed in the age group of 3–4 years, and males (stallions and geldings) were significantly more frequently infected than females. However, neither B. caballi nor B. canis could be identified in the peripheral blood samples of infected horses by PCR. Since most of the B. caballi -positive horses remained negative in the B. canis IFAT, whereas seroconversion solely to B. canis was detected in several regions of the country, serological cross-reaction between the two species can be discounted. This is the first serological evidence of horses being naturally infected with B. canis , supporting the view that piroplasms are less host specific than previously thought.

2010 ◽  
Vol 33 (3) ◽  
pp. 103-106 ◽  
Author(s):  
Md. Tahminur Rahman ◽  
Rosy Sultana ◽  
Sohel Reza Chowdhury

An attempt was made to see the seropositivity and pattern of four common hepatotrophic viruses (HBV, HCV, HEV, HAV) in patients suspected to have been suffering from viral hepatitis. Blood samples from 2,995 cases of suspected HBV infection, 331 from HCV, 155 from HEV and 24 from HAV were tested for these viral markers. Of these a total of 245 (8.1%) were positive for HBsAg, 18 (5.4%) for HCV, 87 (56.1%) for HEV and 8 (33.3%) were positive for HAV infection. The predominant age group affected in HBV is 25-40 years, for HCV >61 years, HEV 26-50 years and HAV under 11 years. Seropositivity for HBsAg was statistically higher (near double) in males than females (p<0.05). HCV showed more seropositivity in males than females. HEV showed slight male preponderance and for HAV the female showed preponderance. The seropositivity rate was higher in Bangladesh in comparison to other developed and developing countries.DOI = 10.3329/bmrcb.v33i3.1142Bangladesh Med Res Counc Bull 2007; 33: 103-106


Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 520 ◽  
Author(s):  
Katiuska Satué ◽  
Esterina Fazio ◽  
Ana Muñoz ◽  
Pietro Medica

In cycling females, the periovulatory period is characterized by stimulation of the hypothalamic pituitary adrenal (HPA) axis. The aim of present study was to analyze the pattern and interrelationships among adrenocorticotropic hormone (ACTH), cortisol (CORT), aldosterone (ALD) and electrolytes (sodium—Na+, potassium—K+ and chloride—Cl−) during periovulatory period in cycling mares. Venous blood samples were obtained daily from a total of 23 Purebred Spanish broodmares, aged 7.09 ± 2.5 years, from day −5 to day +5 of estrous cycle, considering day 0, the day of ovulation. Plasma ACTH was measured by a fluorescent immunoassay kit, serum CORT and ALD by means of a competitive ELISA immunoassay, and plasma Na+, K+ and Cl− were quantified by an analyzer with selective electrodes for the three ions. ACTH showed higher concentrations at day 0 compared to days −5 to −1 and +1 to +3 (p < 0.05). CORT showed higher concentrations at day 0 compared to days −5 to −2 and +1 to +5 (p < 0.05). ALD showed higher concentrations at day 0 compared to days −5 to −2 (p < 0.05) and +2 (p < 0.05). Na+ and Cl− showed higher concentrations at day 0, compared to day −5 and +5. K+ showed lower concentrations at day 0 compared to day +1 (p < 0.05). The significant correlations obtained between ACTH and CORT (r = 0.20) and between ACTH and ALD (r = 0.32) suggest that although ACTH may have an effect both on CORT and ALD, there are other very important determinants that could be considered. Hence, it is possible to presume that the pituitary adrenocortical response and ALD may be involved in the ovulatory mechanisms without a direct relation with electrolyte pattern.


2017 ◽  
Vol 35 (1) ◽  
pp. 52-55 ◽  
Author(s):  
Muhammad Irfan Habib ◽  
Khalid Mehmood A Khan

ObjectiveTo determine the clinical profile and outcome of critically ill children presenting to a paediatric ED in a lower middle-income country.MethodsWe performed a retrospective analysis of children (<14 years) presenting to the ED of the National Institute of Child Health, Karachi, between January and December 2014 who were assigned to acuity 1 (requiring immediate life-saving interventions) according to the Emergency Severity Index. Data included demographic variables, presenting complaints, interventions and outcomes in the ED.ResultsThere were 172 162 visits during the year. Of these, 13 551 (8%) were level 1. 64% of level 1 patients were transported to the ED without ambulance service. Neonates (0–28 days) constituted 48% of level 1 children; their most frequent presenting complaints were respiratory symptoms, followed by fever and reluctance to feed. Above the neonatal age group, the most common presenting complaints were gastrointestinal symptoms (with signs of hypoperfusion), followed by seizures, reluctance to feed and respiratory symptoms. 64% of children of >28 days presenting were malnourished. Interventions included cardiopulmonary resuscitation, application of bubble continuous positive airway pressure and endotracheal intubation. Overall mortality was 13%; 63% of all deaths were in the neonatal age group.ConclusionChildren with the highest triage acuity represent 8% of all visits to a paediatric ED. In this group, neonates account for nearly half of all the children, and more than half of all the deaths among critically ill children came in ED. A large proportion of high-acuity children are malnourished.


Author(s):  
D.E. Kenny ◽  
K. Mobley ◽  
S. Hinkle ◽  
C. Bickel ◽  
F. Knightly ◽  
...  

Since 2002 the Denver Zoological Foundation has produced 28 African hunting dog (Lycaon Pictus) puppies in 3 litters (7, 14 and 7 pups) from the same dam and sire. Wellness examinations were performed on each puppy. The wellness examinations spanned the range of 6-14 weeks of age. During the wellness examinations, in addition to physical examinations and vaccinations, blood samples for complete blood counts and sera biochemistry were obtained.Weights, morphometric measurements, rectal cultures for enteric pathogens and dental eruption patterns were recorded. Blood samples from each age group were compared with adult values from the Denver Zoo. It was noted that animals from the 14-pup litter were 63.6 % of the mean weight of the two 7-pup litters, but size differences (in, for example, total bodylength) were less apparent. Two organisms were recovered from rectal cultures, namely Yersinia enterocolitica (n = 2) and Plesiomonas shigelloides (n = 3). The following deciduous eruption patterns were also noted; at 6 weeks, I1-3, i1-3, C1, c1, P1-2 and p1-2 (n=7) were present, at 9-10 weeks, P3 and p3 (n=21) , and finally at 12-14 weeks, P4 (n = 28).


2013 ◽  
Vol 20 (03) ◽  
pp. 341-347
Author(s):  
SHAHANA ARSHI ◽  
FAKHRA NAHEED ◽  
MAZHAR BADSHAH ◽  
Farah Naz ◽  
Kamran Sardar

Objective: To see the frequency of diabetes mellitus and impaired fasting glucose levels in hypertensive patients. Design:Descriptive study. Place of Study: OPD / Filter clinic. Pakistan Institute of Medical Sciences. Islamabad. Patients and Methods: Thisstudy was carried out on 116 hypertensive patients during the period from August to September '2008. Out of 116 patients, 64 werefemales and 52 were males with age group between 20 to 70 years. Secondary causes are ruled out on the basis of history and physicalexamination. Blood samples were sent to PIMS laboratory for fasting glucose. Data and results were analyzed in SPSS. Results:According to this study, out of 116 patients, there were18 patients who had diabetes mellitus while 33 had hypertension with impairedfasting glucose tolerance which is statistically significant. The females as compared to males were increased in number who had bothdiabetes mellitus and impaired fasting glucose. According to age diabetes mellitus increased in age groups between 41-50 and 61-70years while impaired fasting glucose were increased in age group between 31-60 yrs. The results indicate that impaired fasting glucose ispresent in significant number of hypertensive patients. Conclusions: Hypertension is associated with diabetes mellitus and impairedfasting glucose.


2019 ◽  
Vol 01 (04) ◽  
pp. 20-28
Author(s):  
Aqib Nazeer ◽  
Shahid Ali ◽  
Imran Tipu

Background The prevalence of hepatitis B virus (HBV) in the Pakistani population has been reported previously, however, studies with a city-oriented approach and focus on age and gender distribution are very limited. Therefore, the current study was designed to unravel the age-wise and gender wise prevalence of HBV in Lahore, Pakistan. Methods A total of 350 blood samples of both male and female patients who visited National Genetic Laboratory, Lahore between February 2019 and July 2019 and who were suspected of HBV infection were screened. Sandwich based ELISA was used to detect rapid hepatitis B surface antigen (HbsAg) according to the manufacturer’s instruction. Real time PCR was used to detect HBV using HBV Rotor Gene PCR kit. Results Out of 350 blood samples screened for HBV infection (n= 350), 180 (51.43%) were of males and 170 (48.57%) were of females. Mean age (years) with SD (standard deviation) of the screened population was 37.22 ± 12.16 years. Overall, 224 samples (64%) were found to be positive for HBV infection. In our study, the number of females with this infection (52.24%) was slightly higher than males (47.76%). However, we observed no statistically significant difference (p = 0.225) between them. Conclusion Our study concludes that HBV is highly prevalent in Lahore, Pakistan. Females are slightly more susceptible to HBV infection as compared to males. This study also reports that HBV is more prevalent in the 20-40 age group.


Author(s):  
Kabita Kumari Sahu

The objective of the chapter is to analyze the performance and constraints of selected occupation-related SHGs in India and examine the sustainability of SHGs providing microfinance on the basis of secondary data and primary data from Kendrapara district of Odisha, India. It is observed that majority of the SHG members are in young age group with low level of literacy, small and medium level of land holding, medium level of dependency ratio, forming experience, family encouragement, deferred gratification, risk willingness achievement motivation, and economic activities. The major variables that have contributed for enhancing the performance of SHGs are family encouragement, risk willingness, achievement motivation, extension participation, extension contact, and training programs. In order to enhance further motivation and build confidence, proper market linkage and training programs need to be established. Further, to ensure sustainable development of SHGs in future, federation of SHGs can be thought of by the implementing agency.


1988 ◽  
Vol 7 (4) ◽  
pp. 333-336 ◽  
Author(s):  
D.S. Rupa ◽  
P. Rita ◽  
P.P. Reddy ◽  
O.S. Reddi

1 Twenty-five male workers occupationally exposed to DDT, BHC malathion, parathion, dimethoate, fenitrothion, urea and gromor were selected as subjects for the analysis of chromosomal aberrations and sister chromatid exchanges (SCE) in peripheral lymphocytes. 2 Blood samples were collected from 30 normal healthy males from the same age group and socioeconomic class for the control. 3 The frequency of chromosomal aberrations and SCEs increased significantly irrespective of the duration of exposure to pesticides, when compared to controls.


Author(s):  
Taufiq Ahmad ◽  
Rifat Haneef

This study investigated the Socio-economic and Socio-personal characteristics of agripreneurs. The study was conducted in Uttarakhand and Punjab states. Data for the investigation were collected from 120 respondent trainees, 60 each from the selected state. The study discovered that the majority of respondents (65.83) belonged to middle age group of 29-40 years, were married (75.83%) and had a low level of experience (0-5 years) in the agri enterprises. It was found that the majority of the respondents (50.83%) were engaged in agriculture belonged to the General caste and had education up to graduation (57.50%). A maximum number of the respondents were involved in social and political institutions holding more than one position (35.83%), belonged to medium family size (49.17%), possessed 5-10 animal (biogas plant, pump set, two-wheeler (36.67%). The majority of the respondents (81.17%) had a medium level of socio-economic status (SES).


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