scholarly journals Initial performance of maize in response to humic acids and plant growth-promoting bacteria

Revista CERES ◽  
2018 ◽  
Vol 65 (3) ◽  
pp. 271-277 ◽  
Author(s):  
Raphael Oliveira de Melo ◽  
Hend Pereira de Oliveira ◽  
Klever Cristiano Silveira ◽  
Lílian Estrela Borges Baldotto ◽  
Marihus Altoé Baldotto
Keyword(s):  
Plant Growth ◽  
Humic Acids ◽  
Most Probable Number ◽  
Plant Growth Promoting Bacteria ◽  
Probable Number ◽  
Combined Application ◽  
Maize Seeds ◽  
Burkholderia Gladioli ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT Seed treatment with inoculants based on plant growth-promoting bacteria (PGPB) or the application of humic acids (HA) may increase the productivity of plants of agricultural interest. The hypothesis of this work is that it is possible to combine the effect of plant growth promoting characteristic of HA with the inoculation of PGPB selected strains in the treatment of maize seeds. Thus, providing superior responses than in single applications of both in the initial maize development. To meet this purpose, we conducted isolated application of HA or PGPB inoculation of Burkholderia gladioli and Rhizobium cellulosilyticum, and the combined application of PGPB and HA for treatment of maize seeds. At the end of the experiment (45 days after germination), the plants were evaluated biometrically, nutritionally and a bacteria count was performed in plants using the Most Probable Number technique. The results showed that it is possible to combine the effects of HA with the inoculation of selected strains of PGPB, obtaining superior responses to the isolated application of both. Thus, the use of HA-based bio-stimulants in combination with PGPB is positive and complementary compared to inputs generally used in the treatment of maize seeds.

scholarly journals INOCULATION AND ISOLATION OF PLANT GROWTH-PROMOTING BACTERIA IN MAIZE GROWN IN VITÓRIA DA CONQUISTA, BAHIA, BRAZIL

2015 ◽  
Vol 39 (1) ◽  
pp. 78-85 ◽  
Author(s):  
Joelma da Silva Santos ◽  
Tarciana de Oliveira Viana ◽  
Cristina Meira de Jesus ◽  
Vera Lúcia Divan Baldani ◽  
Joilson Silva Ferreira
Keyword(s):  
Plant Growth ◽  
Nitrogenase Activity ◽  
Most Probable Number ◽  
Acetylene Reduction Activity ◽  
Plant Growth Promoting Bacteria ◽  
Dry Matter Accumulation ◽  
Total N ◽  
Probable Number ◽  
Plant Growth Promoting ◽  
Growth Promoting

Maize is among the most important crops in the world. This plant species can be colonized by diazotrophic bacteria able to convert atmospheric N into ammonium under natural conditions. This study aimed to investigate the effect of inoculation of the diazotrophic bacterium Herbaspirillum seropedicae (ZAE94) and isolate new strains of plant growth-promoting bacteria in maize grown in Vitória da Conquista, Bahia, Brazil. The study was conducted in a greenhouse at the Experimental Area of the Universidade Estadual do Sudoeste da Bahia. Inoculation was performed with peat substrate, with and without inoculation containing strain ZAE94 of H. seropedicae and four rates of N, in the form of ammonium sulfate (0, 60, 100, and 140 kg ha-1 N). After 45 days, plant height, dry matter accumulation in shoots, percentage of N, and total N (NTotal) were evaluated. The bacteria were isolated from root and shoot fragments of the absolute control; the technique of the most probable number and identification of bacteria were used. The new isolates were physiologically characterized for production of indole acetic acid (IAA) and nitrogenase activity. We obtained 30 isolates from maize plants. Inoculation with strain ZAE94 promoted an increase of 14.3 % in shoot dry mass and of 44.3 % in NTotal when associated with the rate 60 kg ha-1 N. The strains N11 and N13 performed best with regard to IAA production and J06, J08, J10, and N15 stood out in acetylene reduction activity, demonstrating potential for inoculation of maize.

scholarly journals Attenuations of bacterial spot disease Xanthomonas euvesicatoria on tomato plants treated with biostimulants

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Aminthia Pombo Sudré da Silva ◽  
Fábio Lopes Olivares ◽  
Cláudia Pombo Sudré ◽  
Lázaro Eustáquio Pereira Peres ◽  
Natália Aguiar Canellas ◽  
...  
Keyword(s):  
Plant Growth ◽  
Organic Acids ◽  
Disease Progression ◽  
Humic Acids ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Spot ◽  
Spot Disease ◽  
Plant Growth Promoting ◽  
Bacterial Spot Disease ◽  
Growth Promoting

Abstract Background The bacterial-spot disease caused by different Xanthomonas species is one of the major tomato diseases that reduce crop production and quality. Pesticides indiscriminate usage has resulted in an increase in resistant bacterial strains as well as contamination of farmers, consumers and the environment. Plant growth-promoting bacteria and humic acids can act as elicitors of plant defence mechanism causing extensive transcriptional and metabolic reprogramming which, in turn, produce a range of plant chemical defences. The purpose of this study was to study how humic acids and plant growth-promoting bacteria, when applied to the substrate, affected the severity of bacterial spot symptoms in tomato leaves. Materials and methods One-month-old Micro-Tom tomato (Solanum lycopersicum L.) were transferred to 3 L pots filled with a sterile mixture of sand and vermiculite (2:1, v:v) and treated or not (control) with 250 mL of 4.5 mmol C. L−1 of humic acids, Herbaspirillum seropedicae (108 CFU. mL−1) and the combination of humic acids plus H. seropedicae. One day after substrate treatment, the leaves were inoculated (or not) with X. euvesicatoria (Xe). The area below the disease progression curve based on severity scores and the number of symptomatic leaflets was used to assess phytopathogen virulence. The concentration of oxalic, citric and succinic acids in leaf extracts were determined using HPLC analysis. Results Sole or combined H. seropedicae (BAC) and humic acids (HA) application promoted shoot and root growth related to control when plants were challenged with Xe pathogen. For plants inoculated with Xe, more significant plant-growth promotion results were obtained for HA + BAC treatment. The first visible symptoms were observed 16 days after inoculation with 2 × 104 CFU. g−1 of Xe cells in leaves of control plants. HA and BAC applied alone or combined reduced disease severity. Only plants treated with HA were able to reduce disease incidence (number of the leaflets with symptoms). Organic acids, such as oxalic, citric and succinic acids, rose in Xe-inoculated leaves. The reduced amount of organic acids in diseased leaves treated with HA + BAC may be linked to a decrease in disease progression. Conclusion Humic acids and H. seropedicae increased growth by modulating the content of organic acids in leaf tissue, attenuating the symptoms of the bacterial spot disease. Graphic abstract

scholarly journals Compatibility of Azospirillum brasilense with Pesticides Used for Treatment of Maize Seeds

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Mariana S. Santos ◽  
Artur B. L. Rondina ◽  
Marco A. Nogueira ◽  
Mariangela Hungria
Keyword(s):  
Plant Growth ◽  
Root Hair ◽  
Azospirillum Brasilense ◽  
Plant Protection ◽  
Plant Growth Promoting Bacteria ◽  
Root Volume ◽  
Maize Seeds ◽  
Plant Growth Promoting ◽  
Growth Promoting

Seed treatment with chemical pesticides is commonly used as an initial plant protection procedure against pests and diseases. However, the use of such chemicals may impair the survival and performance of beneficial microorganisms introduced via inoculants, such as the plant growth-promoting bacterium Azospirillum brasilense. We assessed the compatibility between the most common pesticide used in Brazil for the treatment of maize seeds, composed of two fungicides, and one insecticide, with the commercial strains Ab-V5 and Ab-V6 of A. brasilense, and evaluated the impacts on initial plant development. The toxicity of the pesticide to A. brasilense was confirmed, with an increase in cell mortality after only 24 hours of exposure in vitro. Seed germination and seedling growth were not affected neither by the A. brasilense nor by the pesticide. However, under greenhouse conditions, the pesticide affected root volume and dry weight and root-hair incidence, but the toxicity was alleviated by the inoculation with A. brasilense for the root volume and root-hair incidence parameters. In maize seeds inoculated with A. brasilense, the pesticide negatively affected the number of branches, root-hair incidence, and root-hair length. Therefore, new inoculant formulations with cell protectors and the development of compatible pesticides should be searched to guarantee the benefits of inoculation with plant growth-promoting bacteria.

scholarly journals A Multifactorial Approach to Untangle Graphene Oxide (GO) Nanosheets Effects on Plants: Plant Growth-Promoting Bacteria Inoculation, Bacterial Survival, and Drought

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 771
Author(s):  
Tiago Lopes ◽  
Catarina Cruz ◽  
Paulo Cardoso ◽  
Ricardo Pinto ◽  
Paula A. A. P. Marques ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Plant Growth ◽  
Food Production ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Survival ◽  
Maize Seedlings ◽  
Combined Application ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Rhizobium Sp

Drought is a limiting factor for agricultural productivity. Climate change threatens to expand the areas of the globe subjected to drought, as well as to increase the severity and duration of water shortage. Plant growth-promoting bacteria (PGPB) are widely studied and applied as biostimulants to increase plant production and to enhance tolerance to abiotic and biotic constraints. Besides PGPB, studies on the potential of nanoparticles to be used as biostimulants are also thriving. However, many studies report toxicity of tested nanoparticles in bacteria and plants in laboratory conditions, but few studies have reported effects of nanoparticles towards bacterial cells and communities in the soil. The combined application of nanoparticles and PGPB as biostimulant formulations are poorly explored and it is important to unravel the potentialities of their combined application as a way to potentiate food production. In this study, Rhizobium sp. E20-8 and graphene oxide (GO) nanosheets were applied on container-grown maize seedlings in watered and drought conditions. Bacterial survival, seedling growth (dry weight), and biochemical endpoints (photosynthetic pigments, soluble and insoluble carbohydrates, proline, lipid peroxidation, protein, electron transport system, and superoxide dismutase) were evaluated. Results showed that the simultaneous exposure to GO and Rhizobium sp. E20-8 was able to alleviate the stress induced by drought on maize seedlings through osmotic and antioxidant protection by GO and mitigation of GO effects on the plant’s biochemistry by Rhizobium sp. E20-8. These results constitute a new lead on the development of biostimulant formulations to improve plant performance and increase food production in water-limited conditions.

scholarly journals Use Of Plant Growth-Promoting Bacteria And Humic Acids For Phytostabilization of Acid-Generating Mining Wastes

2021 ◽  
Author(s):  
Svetlana Bratkova ◽  
Katerina Nikolova ◽  
Petya Genova ◽  
Anatoliy Angelov
Keyword(s):  
Humic Acid ◽  
Plant Growth ◽  
Humic Acids ◽  
Mine Drainage ◽  
Cost Effective ◽  
Drainage Water ◽  
Plant Growth Promoting Bacteria ◽  
Mining Wastes ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Generation of acidic mine drainage is a major environmental problem in areas with mining waste. The cost-effective method for reclaiming of acid-generating mining wastes is a vegetation cover. Using plant growth-promoting bacteria (PGPR) and humic acid in remediation have several beneficial effects. The application of both humic substances and PGPR resulted in a decrease in Cu, Fe, Zn and sulfate concentrations in a variety of drainage water samples. Both PGPR and humic acid improve plant growth when used separately, however, the combination of both treatments has the most positive effect on fresh biomass yield – between 22% and 43 % and dry biomass of plants – between 31% and 41 %. Furthermore, addition of Bacillus and Pseudomonas bacteria, in combination with humic acids, to poor soil for reclamation improved grass mineral nutrition and reduced Cu and Zn uptake. The treatment with PGPR and humic acids significantly increased the uptake of nitrogen, phosphorus and potassium by plants.

scholarly journals Maize response to inoculation with strains of plant growth-promoting bactéria

2016 ◽  
Vol 20 (7) ◽  
pp. 606-611 ◽  
Author(s):  
Janaína Dartora ◽  
Vandeir F. Guimarães ◽  
Cid R. J. Menezes ◽  
Mariângela B. Freiberger ◽  
Gustavo Castoldi ◽  
...  
Keyword(s):  
Plant Growth ◽  
Block Design ◽  
N Fertilization ◽  
Plant Growth Promoting Bacteria ◽  
Maize Seeds ◽  
Randomized Block Design ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Maize Response ◽  
Rhizobium Sp

ABSTRACT The aim of this study was to evaluate the response of maize to inoculation with strains of plant growth-promoting bacteria (PGPB) in two cultivation years. The experiment was set in a randomized block design with four replicates in two cultivation years (2012/13 and 2013/14). The treatments consisted of PGPB inoculation: control (without N and without inoculation); 30 kg of N ha-1 at sowing (N1); 160 kg of N ha-1 (N1 + 130 kg of N ha-1 as top-dressing); N1 + A. brasilense, Ab-V5; N1 + A. brasilense, HM053; N1 + Azospirillum sp. L26; N1 + Azospirillum sp. L27; N1 + Enhydrobacter sp. 4331; N1 + Rhizobium sp. 8121. Basal stem diameter, plant height, leaf area, shoot dry matter and yield were evaluated. The strain of Rhizobium sp. 8121and the isolate Azospirillum sp. L26 associated with 30 kg of N ha-1 at sowing promoted yields equivalent to that of the N fertilization of 160 kg ha-1, demonstrating the potential to be used in the inoculation of maize seeds.

scholarly journals Bacterial Consortium for Improved Maize (Zea mays L.) Production

2019 ◽  
Vol 7 (11) ◽  
pp. 519 ◽  
Author(s):  
Oluwaseyi Samuel Olanrewaju ◽  
Olubukola Oluranti Babalola
Keyword(s):  
Plant Growth ◽  
Growth Parameters ◽  
Block Design ◽  
Bacterial Consortium ◽  
Microbial Consortia ◽  
Plant Growth Promoting Bacteria ◽  
Promoting Effect ◽  
Maize Seeds ◽  
Plant Growth Promoting ◽  
Growth Promoting

The ever-increasing human population is a major concern for food security. Maize is the third largest most important food crop. The major problems of cultivation arise from urbanization and land pollution. This reduces the amount of land available for agriculture. The use of chemicals in agriculture is not environmentally friendly. Thus, plant growth-promoting bacteria (PGPB) have been proposed as alternatives. This study aims to test the growth-promoting effect of maize inoculated with six indigenous PGPB isolates. These isolates were assayed for various biochemical and plant growth-promoting activities. They were also assayed for biocontrol activities. Based on the results, six isolates viz A1, A18, A29, NWU4, NWU14, and NWU198 were used to inoculate maize seeds. The inoculated seeds were tried out on the field. A randomized block design was used. PGPB used were in single, consortia of two, and three organisms. The length of the leaves, roots, and stem, plant height, numbers of leaves, and weight of 100 seeds were taken at the fourth and eighth weeks after planting. Microbial consortia increased growth parameters compared to single inoculant treatments. Thus, they can be of advantage in the eradication of low yield. They can also serve as reliable alternatives to chemical fertilizers.

Isolation and identification of plant growth-promoting bacteria associated with tall fescue

2009 ◽  
pp. 211-216 ◽  
Author(s):  
J. Monk ◽  
E. Gerard ◽  
S. Young ◽  
K. Widdup ◽  
M. O'Callaghan
Keyword(s):  
New Zealand ◽  
Plant Growth ◽  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Plant Growth Promoting Bacteria ◽  
Beneficial Bacteria ◽  
Isolation And Identification ◽  
Naturally Occurring ◽  
Plant Growth Promoting ◽  
Growth Promoting

Tall fescue (Festuca arundinacea) is a useful alternative to ryegrass in New Zealand pasture but it is slow to establish. Naturally occurring beneficial bacteria in the rhizosphere can improve plant growth and health through a variety of direct and indirect mechanisms. Keywords: rhizosphere, endorhiza, auxin, siderophore, P-solubilisation

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