scholarly journals Dragon's Blood Sap (Croton Lechleri) As Storage Medium For Avulsed Teeth: In Vitro Study Of Cell Viability

2016 ◽  
Vol 27 (6) ◽  
pp. 751-756 ◽  
Author(s):  
Christine Men Martins ◽  
Elizane Ferreira Hamanaka ◽  
Thayse Yumi Hoshida ◽  
Ana Maria Sell ◽  
Mirian Marubayashi Hidalgo ◽  
...  
Keyword(s):  
Cell Viability ◽  
Periodontal Ligament ◽  
Trypan Blue ◽  
In Vitro Study ◽  
Vitro Study ◽  
Periodontal Ligament Cells ◽  
Storage Medium ◽  
Dragon’S Blood ◽  
Storage Media

Abstract Tooth replantation success depends on the condition of cementum periodontal ligament after tooth avulsion; which is influenced by storage medium. The dragon's blood (Croton lechleri) sap has been suggested as a promising medium because it supports collagen formation and exhibits healing, anti-inflammatory and antimicrobial properties. Thus, the aim of this study was to evaluate the efficacy of dragon's blood sap as a storage medium for avulsed teeth through evaluation of functional and metabolic cell viability. This in vitro study compared the efficacy of different storage media to maintain the viability of human peripheral blood mononuclear and periodontal ligament cells. A 10% dragon's blood sap was tested while PBS was selected as its control. Ultra pasteurized whole milk was used for comparison as a commonly used storage medium. DMEM and distilled water were the positive and negative controls, respectively. The viability was assessed through trypan blue exclusion test and colorimetric MTT assay after 1, 3, 6, 10 and 24 h of incubation. The dragon's blood sap showed promising results due to its considerable maintenance of cell viability. For trypan blue test, the dragon's blood sap was similar to milk (p<0.05) and both presented the highest viability values. For MTT, the dragon's blood sap showed better results than all storage media, even better than milk (p<0.05). It was concluded that the dragon's blood sap was as effective as milk, the gold standard for storage medium. The experimental sap preserved the membrane of all cells and the functional viability of periodontal ligament cells.

Potential of the propolis as storage medium to preserve the viability of cultured human periodontal ligament cells: an in vitro study

2011 ◽  
Vol 27 (2) ◽  
pp. 102-108 ◽  
Author(s):  
Payal Saxena ◽  
Vandana Aditya Pant ◽  
Kulvindar Kaur Wadhwani ◽  
Mahendra Pratap Kashyap ◽  
Saurabh Kumar Gupta ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
In Vitro Study ◽  
Vitro Study ◽  
Periodontal Ligament Cells ◽  
Storage Medium ◽  
Human Periodontal Ligament Cells

Coconut milk and probiotic milk as storage media to maintain periodontal ligament cell viability: an in vitro study

2016 ◽  
Vol 33 (3) ◽  
pp. 160-164 ◽  
Author(s):  
Divya Saini ◽  
Prahlad Gadicherla ◽  
Prakash Chandra ◽  
Latha Anandakrishna
Keyword(s):  
Cell Viability ◽  
Periodontal Ligament ◽  
In Vitro Study ◽  
Coconut Milk ◽  
Vitro Study ◽  
Periodontal Ligament Cell ◽  
Storage Media

scholarly journals Anti-inflammatory effects of low-level laser therapy on human periodontal ligament cells: in vitro study

2017 ◽  
Vol 33 (3) ◽  
pp. 469-477 ◽  
Author(s):  
Ji-Hua Lee ◽  
Min-Hsuan Chiang ◽  
Ping-Ho Chen ◽  
Mei-Ling Ho ◽  
Huey-Er Lee ◽  
...  
Keyword(s):  
Laser Therapy ◽  
Periodontal Ligament ◽  
In Vitro Study ◽  
Vitro Study ◽  
Low Level Laser Therapy ◽  
Periodontal Ligament Cells ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser

Influence of Storage Media Containing Salvia officinalis on Survival of Periodontal Ligament Cells

2008 ◽  
Vol 9 (6) ◽  
pp. 17-24 ◽  
Author(s):  
Fatih Ozan ◽  
Zübeyde Akin Polat ◽  
Bektaş Tepe ◽  
Kürşat Er
Keyword(s):  
Cell Viability ◽  
Periodontal Ligament ◽  
Tap Water ◽  
Salvia Officinalis ◽  
The Other ◽  
Periodontal Ligament Cells ◽  
Storage Medium ◽  
Trypan Blue Exclusion ◽  
Storage Media ◽  
Pdl Cells

Aim The purpose of this study was to determine the ability of Salvia officinalis (S. officinalis) extracts to serve as a storage medium for the maintenance of periodontal ligament (PDL) cell viability of avulsed teeth. Methods and Materials PDL cells were obtained from healthy third molars and cultured in Dulbecco's Modified Eagle's Medium (DMEM). Cultures were subjected to 4, 2.5, 1.5, and 0.5% S. officinalis solutions, Hank's balanced salt solution (HBSS), phosphate buffered saline (PBS), and tap water. Tissue culture plates were incubated with experimental media at 37°C for 1, 3, 6, 12 or 24 hours. PDL cell viability was assessed by trypan blue exclusion. Statistical analysis of the data was performed by one-way analysis of variance (ANOVA) complemented by the Tukey's test. The level of significance was 5% (p< 0.05). Results The results showed 2.5% S. officinalis was a more effective storage medium than the other experimental solutions (p<0.05). Only at 1 hour and 3 hours was there found similar effect between 2.5% S. officinalis and HBSS. At 24 hours, 2.5% S. officinalis was found to be significantly better than the other solutions tested. Conclusion S. officinalis can be recommended as a suitable transport medium for avulsed teeth. Clinical Significance The findings of this study support the use of S. officinalis as another option for clinicians to use to store and transport avulsed teeth until reimplantation procedures can be done. Citation Özan F, Polat ZA, Tepe B, Er K. Influence of Storage Media Containing Salvia officinalis on Survival of Periodontal Ligament Cells. J Contemp Dent Pract 2008 September; (9)6:017-024.

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