scholarly journals Cultivable cellulolytic fungi isolated from the gut of Amazonian aquatic insects

2020 ◽  
Vol 50 (4) ◽  
pp. 346-354
Author(s):  
Enide Luciana BELMONT-MONTEFUSCO ◽  
Lorena NACIF-MARÇAL ◽  
Enedina Nogueira de ASSUNÇÃO ◽  
Neusa HAMADA ◽  
Carlos Gustavo NUNES-SILVA
Keyword(s):  
Filamentous Fungi ◽  
Aquatic Insects ◽  
Extracellular Enzymes ◽  
Agar Medium ◽  
Synthetic Medium ◽  
Aquatic Insect ◽  
Cellulolytic Activity ◽  
Insect Larvae ◽  
Potato Dextrose Agar Medium ◽  
Aquatic Insect Larvae

ABSTRACT Filamentous fungi have been targeted by bioprospecting studies because they are effective producers of extracellular enzymes that can potentially be used by the bioindustry. In this study, we isolated filamentous fungi from the guts of Amazonian aquatic insect larvae to evaluate their cellulolytic activity. We collected 69 larvae of shredder insects of three genera: Phylloicus (Trichoptera: Calamoceratidae), Triplectides (Trichoptera: Leptoceridae) and Stenochironomus (Diptera: Chironomidae) in ten streams from a protected area in the central Brazilian Amazon. Production of mycelia was elaborated in PDA (Potato Dextrose Agar) medium. The isolates were transferred to a synthetic medium with carboxymethyl cellulose, and Congo red was used to determine the enzymatic index. The hydrolysis halo, indicating the production of cellulases, was observed in 175 fungal isolates (70% of the total), of which 25 had an enzymatic index ≥ 2.0 and belonged to seven fungal genera. The fungal taxa Cladosporium, Gliocephalotrichum, Penicillium, Pestalotiopsis, Talaromyces, Trichoderma and Umbelopsis were isolated from guts of Phylloicus, Triplectides and Stenochironomus, which are traditionally used in biotechnological applications. Our results indicate the cellulolytic potential of fungi associated with the guts of aquatic Amazonian insects.

In vitro infection experiments with eggs of the nematode Contracaecum rudolphii Hartwich, 1964 (sensu lato) targeting aquatic insect larvae (Odonata: Coenagrionidae and Libellulidae; Trichoptera: Integripalpia) as possible intermediate hosts

2014 ◽  
Vol 43 (2) ◽  
Author(s):  
Janina Dziekońska-Rynko ◽  
Jerzy Rokicki ◽  
Katarzyna Mierzejewska
Keyword(s):  
Aquatic Insects ◽  
Aquatic Insect ◽  
Intermediate Hosts ◽  
Insect Larvae ◽  
Contracaecum Rudolphii ◽  
High Prevalence ◽  
Nematode Eggs ◽  
Newly Hatched Larvae ◽  
Aquatic Insect Larvae

AbstractThe availability of aquatic insects (Odonata: Coenagrionidae, Libellulidae and Trichoptera: Integripalpia) as potential intermediate hosts for the nematode Contracaecum rudolphii Hartwich, 1964 sensu lato was studied under laboratory conditions. The infective material consisted of nematode eggs, newly hatched larvae, as well as in vitro infected cyclopoid copepods. High prevalence and intensity of infection associated with a low mortality of aquatic insect larvae suggests that they may serve as intermediate hosts for C. rudolphii and constitute a major reservoir of C. rudolphii larvae in aquatic habitats.

scholarly journals Effects of bromeliad flowering event on the community structuring of aquatic insect larvae associated with phytotelmata of Aechmea distichantha Lem. (Bromeliaceae)

2017 ◽  
Vol 29 (0) ◽  
Author(s):  
Felipe Emiliano Amadeo ◽  
Juliana Déo Dias ◽  
Bianca Trevizan Segovia ◽  
Nadson Ressyé Simões ◽  
Fábio Amodêo Lansac-Tôha
Keyword(s):  
Growth Phase ◽  
Vegetative Growth ◽  
Beta Diversity ◽  
Aquatic Insect ◽  
Insect Larvae ◽  
Local Factors ◽  
Flowering Phase ◽  
Regional Factors ◽  
Aquatic Insect Larvae ◽  
Phenological Phases

Abstract Aim: We aimed to understand how aquatic insect larvae communities associated with bromeliad phytotelmata are affected by plant architecture, predators and resources (local factors), and by geographical distance (regional factors) in two different plant phenological phases. Bromeliad flowering results in plant structural changes, which favours insect dispersal. Considering that local and regional factors may affect the community of aquatic insect larvae, we expected that composition, beta diversity and the importance of those factors would differ in the vegetative growth and flowering phases. Methods We performed six samplings of the bromeliad associated fauna in 2010, three during the first semester - vegetative growth phase - and three during the second semester - flowering phase. In each sampling, we collected 12 plants along the rocky walls with similar location distribution, with a total of 72 bromeliads studied. Results Although beta diversity (PERMDISP) did not differ between vegetative growth and flowering, NMDS followed by ANOSIM showed that composition was significantly different in the distinct phenological phases. IndVal results showed that three Diptera morphospecies were discriminant of the vegetative growth phase. In addition, pRDA revealed differences in the relative contribution of local and regional factors to explain insect larvae community structure. During the flowering phase, local factors predominated, while during vegetative growth, regional factors were more important. Conclusion Differences in dispersal rates between the two phenological phases, likely due to adult insect pollination and further oviposition, influenced community structuring. Therefore, flowering events account for differences not only in the composition, but also in community structuring of aquatic insect larvae inhabiting the phytotelmata of Aechmea distichantha Lem. (Bromeliaceae).

scholarly journals Trichomycetes in Argentinean Aquatic Insect Larvae

Mycologia ◽  
1999 ◽  
Vol 91 (6) ◽  
pp. 1060 ◽  
Author(s):  
Robert W. Lichtwardt ◽  
Leonard C. Ferrington ◽  
Claudia Lopez Lastra
Keyword(s):  
Aquatic Insect ◽  
Insect Larvae ◽  
Aquatic Insect Larvae

Trichomycete gut fungi in New Zealand aquatic insect larvae

1990 ◽  
Vol 68 (5) ◽  
pp. 1045-1056 ◽  
Author(s):  
Marvin C. Williams ◽  
Robert W. Lichtwardt
Keyword(s):  
New Species ◽  
New Zealand ◽  
New Genus ◽  
Aquatic Insect ◽  
The Other ◽  
Insect Larvae ◽  
Widespread Species ◽  
The World ◽  
Chironomidae Larvae ◽  
Aquatic Insect Larvae

New Zealand, like other regions of the world, has now been shown to have a diverse and rich assortment of Trichomycetes (Zygomycotina). Seven of the 14 species of Harpellales we found in aquatic insect larvae are known from other land areas. The remaining seven species, consisting of six Harpellales and one Amoebidiales, are new and possibly endemic. A new genus, Austrosmittium, from Chironomidae larvae is established, with two species, A. kiwiorum and A. norinsulare. The other new species are Glotzia plecopterorum (in Plecoptera), Paramoebidium bibrachium (Amoebidiales, in Ephemeroptera), Pennella asymmetrica (in Simuliidae), and Smittium rarum and Stachylina minima (in Chironomidae). All of the new species were found either on North Island or South Island, but not both. We also report the presence on South Island of two widespread species of marine trichomycetes (Eccrinales), Enteromyces callianassae and Taeniella carcini, in anomuran and brachyuran crustaceans.

Tasmanian Trichomycete Gut Fungi in Aquatic Insect Larvae

Mycologia ◽  
1992 ◽  
Vol 84 (3) ◽  
pp. 384-391 ◽  
Author(s):  
Robert W. Lichtwardt ◽  
Marvin C. Williams
Keyword(s):  
Aquatic Insect ◽  
Insect Larvae ◽  
Aquatic Insect Larvae

Tracheal and Blood Gills in Aquatic Insect Larvæ

Nature ◽  
1933 ◽  
Vol 131 (3311) ◽  
pp. 549-550 ◽  
Author(s):  
W. H. THORPE
Keyword(s):  
Aquatic Insect ◽  
Insect Larvae ◽  
Aquatic Insect Larvae
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