scholarly journals DETECTION OF HTLV-I ANTIBODIES AND DNA IN BLOOD SAMPLE OF A PATIENT WITH MYELOPATHY IN NIGERIA

1998 ◽  
Vol 40 (1) ◽  
pp. 55-58 ◽  
Author(s):  
O. D. OLALEYE ◽  
A. OGUNNIYI ◽  
Zhi Juan SHENG ◽  
Zhiliang LI ◽  
S. RASHEED
Keyword(s):  
Genomic Dna ◽  
Mononuclear Cells ◽  
Lower Limbs ◽  
Type I ◽  
Chain Reaction ◽  
Peripheral Blood Mononuclear ◽  
T Lymphotropic Virus ◽  
Progressive Loss ◽  
Blot Technique ◽  
Polymerase Chain

We describe a case of human T-lymphotropic virus type I associated myelopathy in a 50-year old woman in Nigeria. The patient presented with progressive loss of tone to the two lower limbs and later inability to walk. The HTLV-I antibody presence in the plasma collected from the patient was repeatedly detected by enzyme immunoassays (Abbott HTLV-I EIA and Coulter SELECT-HTLV I/II) and confirmed by Western blot technique. In addition, HTLV-I DNA was amplified from the genomic DNA isolated from the peripheral blood mononuclear cells of the patient by the polymerase chain reaction technique. This finding is significant being the first report of association of HTLV-I with myelopathy in Nigeria.

scholarly journals Prophylaxis against a Melanesian variant of human T-lymphotropic virus type I (HTLV-I) in rabbits using HTLV-I immune globulin from asymptomatically infected Japanese carriers

Blood ◽  
1993 ◽  
Vol 82 (12) ◽  
pp. 3664-3667 ◽  
Author(s):  
Y Tanaka ◽  
K Ishii ◽  
T Sawada ◽  
Y Ohtsuki ◽  
H Hoshino ◽  
...  
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Virus Type ◽  
Neutralizing Antibody ◽  
Type I ◽  
Chain Reaction ◽  
T Lymphotropic Virus ◽  
Molecular Variants ◽  
Vesicular Stomatitis ◽  
Polymerase Chain ◽  
Contaminated Blood

Abstract Molecular variants of human T-lymphotropic virus type I (HTLV-I), which diverge significantly from the so-called cosmopolitan prototypes, have been discovered in Melanesia. In this study, HTLV-I IgG (I-IgG) prepared from seropositive healthy Japanese carriers was evaluated for its protective effect against a Melanesian isolate, HTLV-IMEL5, in rabbits. Normal IgG (N-IgG) prepared from seronegative healthy Japanese was used as control. Both preparations contained 50 mg/mL of IgG and I- IgG had a high neutralizing antibody titer, as determined by vesicular stomatitis virus--HTLV-I pseudotype assay. Of four experimental groups (A, B, C, and D), each with three rabbits, groups A and B were infused with 10 mL of N-IgG and I-IgG, respectively, and animals were challenged immediately by transfusion of 5 mL of blood from a rabbit infected with HTLV-IMEL5. Animals in groups C and D were immunized with 10 mL of I-IgG 24 and 48 hours, respectively, after being transfused with 5 mL of blood from the virus-infected rabbit. HTLV-I infection, as determined by seroconversion and verified by polymerase chain reaction, occurred in all rabbits in groups A and D after 2 to 6 weeks, but in none of the animals in groups B and C. These data indicate that I-IgG is protective against HTLV-IMEL5 infection when administered before or within 24 hours of transfusion with virus-contaminated blood. Moreover, our study shows that the neutralizing domains of the so-called cosmopolitan and Melanesian strains of HTLV-I are functionally indistinguishable.

Inhibition of Human T-Lymphotropic Virus Type I Gene Expression by the Streptomyces-Derived Substance EM2487

2002 ◽  
Vol 13 (3) ◽  
pp. 177-183 ◽  
Author(s):  
X Wang ◽  
M Okamoto ◽  
M Kawamura ◽  
S Izumo ◽  
M Baba
Keyword(s):  
Gene Expression ◽  
Virus Type ◽  
Mononuclear Cells ◽  
Spastic Paraparesis ◽  
Selective Inhibition ◽  
Pcr Analysis ◽  
Type I ◽  
Peripheral Blood Mononuclear ◽  
T Lymphotropic Virus ◽  
Infected Cells

EM2487, a Streptomyces-derived substance, has previously been shown to inhibit HIV-1 replication in both acutely and chronically infected cells. In this study, we found that EM2487 was also a selective inhibitor of human T-lymphotropic virus type I (HTLV-I) replication in persistently infected cells. Its 50% effective concentrations for HTLV-I p19 antigen production were 3.6 and 1.2 μM in MT-2 and MT-4 cells, respectively. However, the compound did not reduce cell proliferation and viability at these concentrations. The 50% cytotoxic concentrations of EM2487 were 30.6 and 5.7 μM in MT-2 and MT-4 cells, respectively. The compound also displayed selective inhibition of HTLV-I production in peripheral blood mononuclear cells obtained from patients with HTLV-I-associated myelopathy/tropical spastic paraparesis. Quantitative reverse transcription PCR analysis revealed that EM2487 selectively suppressed HTLV-I mRNA synthesis in MT-2 cells in a dose-dependent fashion. However, the compound did not inhibit endogenous Tax-induced HTLV-I long terminal repeat-driven reporter gene expression. Furthermore, intracellular Tax accumulation was not suppressed in MT-2 cells exposed to EM2487. These results suggest that the inhibition occurred at the viral transcription level, but it cannot be attributed to the inhibition of the Tax function.

scholarly journals Prophylaxis against a Melanesian variant of human T-lymphotropic virus type I (HTLV-I) in rabbits using HTLV-I immune globulin from asymptomatically infected Japanese carriers

Blood ◽  
1993 ◽  
Vol 82 (12) ◽  
pp. 3664-3667
Author(s):  
Y Tanaka ◽  
K Ishii ◽  
T Sawada ◽  
Y Ohtsuki ◽  
H Hoshino ◽  
...  
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Virus Type ◽  
Neutralizing Antibody ◽  
Type I ◽  
Chain Reaction ◽  
T Lymphotropic Virus ◽  
Molecular Variants ◽  
Vesicular Stomatitis ◽  
Polymerase Chain ◽  
Contaminated Blood

Molecular variants of human T-lymphotropic virus type I (HTLV-I), which diverge significantly from the so-called cosmopolitan prototypes, have been discovered in Melanesia. In this study, HTLV-I IgG (I-IgG) prepared from seropositive healthy Japanese carriers was evaluated for its protective effect against a Melanesian isolate, HTLV-IMEL5, in rabbits. Normal IgG (N-IgG) prepared from seronegative healthy Japanese was used as control. Both preparations contained 50 mg/mL of IgG and I- IgG had a high neutralizing antibody titer, as determined by vesicular stomatitis virus--HTLV-I pseudotype assay. Of four experimental groups (A, B, C, and D), each with three rabbits, groups A and B were infused with 10 mL of N-IgG and I-IgG, respectively, and animals were challenged immediately by transfusion of 5 mL of blood from a rabbit infected with HTLV-IMEL5. Animals in groups C and D were immunized with 10 mL of I-IgG 24 and 48 hours, respectively, after being transfused with 5 mL of blood from the virus-infected rabbit. HTLV-I infection, as determined by seroconversion and verified by polymerase chain reaction, occurred in all rabbits in groups A and D after 2 to 6 weeks, but in none of the animals in groups B and C. These data indicate that I-IgG is protective against HTLV-IMEL5 infection when administered before or within 24 hours of transfusion with virus-contaminated blood. Moreover, our study shows that the neutralizing domains of the so-called cosmopolitan and Melanesian strains of HTLV-I are functionally indistinguishable.

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