scholarly journals Caracterização do Tomato chlorotic spot virus isolado de jiló no Vale do Paraíba, Estado de São Paulo

2002 ◽  
Vol 27 (3) ◽  
pp. 285-291 ◽  
Author(s):  
MARCELO EIRAS ◽  
ALEXANDRE L. R. CHAVES ◽  
ADDOLORATA COLARICCIO ◽  
RICARDO HARAKAVA ◽  
JANSEN DE ARAUJO ◽  
...  
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Sao Paulo ◽  
Ringspot Virus ◽  
São Paulo ◽  
Rt Pcr ◽  
Spot Virus ◽  
Chlorotic Spot ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Das Elisa

Os tospovírus são responsáveis por perdas significativas em diversas culturas, principalmente solanáceas. No município de São José dos Campos (SP), plantas de jiló (Solanum gilo) apresentando sintomas de mosaico, bolhosidades, nanismo e queda acentuada da produção foram coletadas para análise. Visando a caracterização do agente causador dos sintomas, testes biológicos, elétrono microscópicos, sorológicos e moleculares foram realizados. Através de inoculação mecânica em plantas indicadoras das famílias Amaranthaceae, Chenopodiaceae e Solanaceae obtiveram-se resultados típicos aos esperados para tospovírus. Ao microscópio eletrônico de transmissão, observaram-se, em contrastação negativa, partículas pleomórficas com diâmetro entre 80 e 110 nm e em cortes ultra-finos partículas presentes em vesículas do retículo endoplasmático. Através de DAS-ELISA, identificou-se o Tomato chlorotic spot virus (TCSV). A partir de RNA total extraído de folhas infetadas, amplificaram-se, via RT-PCR, fragmentos correspondentes ao gene da proteína do capsídeo (cp) os quais foram seqüenciados e comparados com outros depositados no "GenBank". A homologia de nucleotídeos e aminoácidos deduzidos foi respectivamente de 99 e 95% quando comparada com seqüências de isolados de TCSV. A comparação com as outras espécies do gênero Tospovirus apresentou valores de homologia entre 72 e 84%. Estes resultados confirmam a identidade deste vírus como pertencente à espécie TCSV, que é predominante no Estado de São Paulo e importante patógeno de outras plantas cultivadas. Além disso, variedades de jiló quando inoculadas foram susceptíveis tanto ao TCSV como às espécies Tomato spotted wilt virus (TSWV) e Groundnut ringspot virus (GRSV).

scholarly journals İstanbul İli Adalar İlçesi’nde Hobi Bahçeleri ve Peyzaj Alanlarında Yetiştirilen Süs Bitkilerinde Tospovirüslerin Saptanması

2017 ◽  
Vol 5 (9) ◽  
pp. 1119
Author(s):  
Fatma Şafak ◽  
Muharrem Arap Kamberoğlu
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Ornamental Plants ◽  
Necrotic Spot ◽  
Rt Pcr ◽  
Spot Virus ◽  
Tomato Spotted Wilt ◽  
Hydrangea Macrophylla ◽  
Wilt Virus ◽  
Pittosporum Tobira ◽  
Das Elisa

This study was conducted in order to detect Tomato spotted wilt virus (TSWV), Irish yellow spot virus (IYSV) and Impatients necrotic spot virus (INSV) in ornamental plants growing in hobby gardens and landscaping areas in Adalar district (Büyükada, Heybeliada, Kınalıada and Burgazada) of Istanbul province between 2015 and 2016. During the surveys carried out in that district, the samples were collected from both simptomatologically suspicious ornamental plants and the plants which did not show any symptoms. All of the collected samples (n=150) were firstly tested by Double Antibody Sandwich (DAS) ELISA and none of the samples were found to be infected with TSWV and INSV. The samples detected to be positive with IYSV by ELISA tests were then used in RT-PCR studies. At the RT-PCR using the IYSV-465c; IYSV-239f primer pair, a band with a size of 240 bp was observed for Pittosporum tobira and Hydrangea macrophylla. Therefore, the presence of IYSV infection in Adalar was also confirmed molecularly.

scholarly journals Tomato chlorotic spot virus in hydroponically-grown lettuce in São Paulo State, Brazil

2004 ◽  
Vol 29 (3) ◽  
pp. 307-311 ◽  
Author(s):  
Addolorata Colariccio ◽  
Marcelo Eiras ◽  
Alexandre L. R. Chaves ◽  
Ricardo Harakava ◽  
César M. Chagas
Keyword(s):  
Epidemiological Studies ◽  
Sao Paulo ◽  
São Paulo ◽  
Rt Pcr ◽  
Spot Virus ◽  
Chlorotic Spot ◽  
Molecular Tests ◽  
Hydroponically Grown ◽  
Wilt Virus ◽  
High Degree

In the regions of Campinas and Sumaré, São Paulo, Brazil, hidroponically grown crops of Lettuce (Lactuca sativa) cv. Verônica, which showed virus-like symptoms were examined by electron microscope, biological, serological and molecular tests. Pleomorphic, enveloped particles (80-100 nm in diameter) were always detected in these samples. Experimentally inoculated host plants, including lettuce, reacted with tospoviruses-induced symptoms. Some differences were observed in Gomphrena globosa, which reacted by showing local lesions and systemic mosaic. Two isolates of Tomato chlorotic spot virus (TCSV) were identified by DAS-ELISA and by RT-PCR. The sequencing and alignment of the RT-PCR coat protein amplified fragments have indicated a high degree of homology with the TCSV sequences stored in the GenBank. This is the first report of losses due to a virus from the genus Tospovirus in commercial hydroponic lettuce crops in Brazil. Further epidemiological studies are needed for better understanding the spread of the virus in hydroponic crops, since Tomato spotted wilt virus (TSWV) is reported to spread through the nutritive solution.

scholarly journals Resistencia genética de híbridos de tomate [Solanum lycopersicum L. (Mill.)] al virus del bronceado (TSWV)

1969 ◽  
Author(s):  
Julio Gabriel ◽  
Daniel Sanabria ◽  
Silene Veramendi ◽  
Giovanna Plata ◽  
Ada Angulo ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Tomato Spotted Wilt Virus ◽  
Ringspot Virus ◽  
Spot Virus ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Das Elisa

La presente investigación se realizó en el invernadero y laboratorio de la Fundación PROINPA en Cochabamba - Bolivia en el 2012. El objetivo fue evaluar la resistencia y suscep- tibilidad de plantas a los virus Tomato spotted wilt virus – TSWV, Tomato cholorotic spot virus – TCSV y Groundnut ringspot virus – GRSV en 10 híbridos de tomate mediante evaluación feno- típica y del patrón molecular (marcador SCAR Sw- 421), que distingue los homocigotos y hete- rocigotos resistentes del susceptible. Los resul- tados mostraron que el marcador SW-421 se co- localizó con el gen Sw-5 de resistencia a TSWV. Se observó la presencia de la banda de resistencia (R) para TSWV a 940 bp en las variedades PROINPA 2 (Aguaí) y PROINPA 9 (Bonita) en estado homocigoto dominante (Sw-5/Sw-5). Las variedades PROINPA 1 (Andinita), PROINPA 3 (Arami), PROINPA 4 (Yara), PROINPA 5 (Pintona), PROINPA 6 (Jasuka), y PROINPA 10 (Bola Pera), mostraron la banda resistencia (H) a TSWV a 900-940 bp en estado heterocigoto (Sw-5/Sw-5+). Solamente la variedad PROINPA 7 (Redonda), el padre 71 89S LACHING SW-5 y la variedad Shannon mostraron el gen de suscep- tibilidad (S) al TSWV a 900 bp en estado homo- cigoto recesivo (Sw-5+/Sw-5+). Los análisis de severidad y de DAS-ELISA fueron confirmados con el análisis molecular.

scholarly journals Tomato spotted wilt virus Identified in Desert Rose in Florida

Plant Disease ◽  
2005 ◽  
Vol 89 (5) ◽  
pp. 526-526 ◽  
Author(s):  
S. Adkins ◽  
C. A. Baker
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
The United States ◽  
N Gene ◽  
Enzyme Linked Immunosorbent Assay ◽  
Insect Vector ◽  
Rt Pcr ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Symptomatic Plant ◽  
Das Elisa

Desert rose (Adenium obesum (Forssk.) Roem. & Schult), a member of the family Apocynaceae, is characterized by fleshy stems and leaves and colorful flowers. This exotic ornamental, originally from southeast Africa, is propagated vegetatively and is a perennial in warm climates. Virus-like foliar symptoms, including chlorotic ring and line patterns, were observed in the fall of 2004 on one of five stock plants being maintained in a greenhouse in Fort Pierce, FL. Inclusion body morphology suggested the presence of a Tospovirus in the symptomatic plant, and Tomato spotted wilt virus (TSWV) was specifically identified in this plant using a commercially available double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA; Agdia, Elkhart, IN). TSWV was not detected in symptomless desert rose plants nor was Impatiens necrotic spot virus detected in any of the plants using DAS-ELISA. Graft transmission of TSWV to other desert rose plants was successful. Sequence analysis of a nucleocapsid (N) protein gene fragment amplified by reverse transcription-polymerase chain reaction (RT-PCR) with primers TSWV723 and TSWV722 (1) from total RNA of the symptomatic plant confirmed the diagnosis. Nucleotide and deduced amino acid sequences of a 579-bp region of the RT-PCR product were 95 to 99% and 95 to 100% identical, respectively, to TSWV N-gene sequences in GenBank. No product was amplified from symptomless plants. Since these 3-year-old plants were grown on-site from seed and only expressed symptoms 2 months following damage to the greenhouse by hurricanes Frances and Jeanne, it is likely that viruliferous thrips were introduced from local vegetable or ornamental production areas during or following the storms. To our knowledge, this is the first report of TSWV infection of desert rose in Florida, although TSWV was observed in this plant in Europe approximately 10 years ago (3,4). Because of the wide distribution of TSWV in the United States, the increasing popularity of desert rose, and the recent identification of Cucumber mosaic virus in this host (2), attention to sanitation and insect vector management is merited during desert rose propagation and production. References: (1) S. Adkins and E. N. Rosskopf. Plant Dis. 86:1310, 2002. (2) C. A. Baker et al. Plant Dis. 87:1007, 2003. (3) J. Mertelik et al. Acta Hortic. 432:368, 1996. (4) J. Th. J. Verhoeven and J. W. Roenhorst. Acta Hortic. 377:175, 1994.

scholarly journals First Report of Tomato spotted wilt virus Infecting African Clover (Trifolium tembense) in Georgia

Plant Disease ◽  
2009 ◽  
Vol 93 (2) ◽  
pp. 202-202 ◽  
Author(s):  
N. A. Barkley ◽  
D. L. Pinnow ◽  
M. L. Wang ◽  
G. A. Pederson
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Plant Genetic Resources ◽  
Germplasm Collection ◽  
Rt Pcr ◽  
First Report ◽  
Nucleocapsid Gene ◽  
Weed Host ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Das Elisa

Tomato spotted wilt virus (TSWV; family Bunyaviridae, genus Tospovirus), which is vectored by several species of thrips (order Thysanoptera, family Thripidae), causes a destructive disease that affects many economically important host plants such as tomatoes, peppers, and peanuts. Controlling the spread of this disease is challenging, and currently, only limited strategies are available to prevent and/or control its dissemination, including early diagnosis, destruction of infected material, and elimination of the vector. TSWV has been previously reported in subterranean clover (Trifolium subterraneum), white clover (T. repens), and various unidentified wild clovers (Trifolium spp.) in North America and Australia (1,3), but never before in an African species. T. tembense (Fresen.), an herbaceous annual African clover that is mainly used for grazing, is part of the national germplasm collection housed at the Plant Genetic Resources Conservation Unit in Griffin, GA. TSWV was found naturally infecting several accessions of this species being grown for regeneration in a greenhouse during 2008. Initial putative identification of the virus was done by visual inspection of host symptoms that included ringspots, necrotic and chlorotic local lesions, sometimes mild systemic wilting, and eventually an overall decline of healthy tissue in the infected plants. This was subsequently confirmed by double-antibody sandwich (DAS)-ELISA and reverse transcription (RT)-PCR. Primers (5′-ATGTCTAAGGTTAAGCTC-3′ forward and 5′-TTAAGCAAGTTCTGTGAG-3′ reverse) targeted the nucleocapsid gene of TSWV and amplified an expected product of approximately 800 bp (2). No product was amplified in any of the negative controls. Twenty-six individuals representing twelve plant accessions (PI 517788, 517790, 517792, 517793, 517809, 517832, 517842, 517845, 517851, 517871, 517876, and 517889) were screened for TSWV. Two to three individuals were targeted from each accession. Samples were chosen on the basis of the availability of leaf tissue to perform two diagnostic assays, ELISA and RT-PCR. Samples chosen for this study were all naturally infected by thrips. All but four individuals representing two plant accessions tested positive for the virus. The RT-PCR data substantiated the DAS-ELISA results and confirmed the suspected infection. More than 26% of the positive samples naturally infected by TSWV were further characterized by purifying and sequencing (bidirectionally) the RT-PCR product on an automated CEQ 8000 sequencer (Beckman Coulter, Fullerton, CA). The resulting sequences were aligned and edited using AlignIR (LI-COR, Lincoln, NE). More than 700 bp of sequence data (GenBank Accession No. FJ183743–FJ183746) was compiled and they displayed 98% identity with deposited TSWV nucleocapsid gene sequences in GenBank, with no similarity to any other targets. To our knowledge, this is the first report of TSWV infection in T. tembense. Accessions potentially resistant to TSWV within this species were identified and need to be further substantiated. T. tembense is a wild, native clover in Africa and could serve as a weed host for infection of nearby agronomically important crops. References: (1) I. Bitterlich and L. S. MacDonald. Can. Plant Dis. Surv. 73:137, 1993. (2) R. J. Holguín-Peña and E. O. Rueda-Puente. Plant Dis. 91:1682, 2007. (3) C. R. Wilson. Plant Pathol. 47:171, 1998.

scholarly journals First Report of Vidalia Onion (Allium cepa) Naturally Infected with Tomato spotted wilt virus and Iris yellow spot virus (Family Bunyaviridae, Genus Tospovirus) in Georgia

Plant Disease ◽  
2004 ◽  
Vol 88 (11) ◽  
pp. 1285-1285 ◽  
Author(s):  
S. W. Mullis ◽  
D. B. Langston ◽  
R. D. Gitaitis ◽  
J. L. Sherwood ◽  
A. C. Csinos ◽  
...  
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Thrips Tabaci ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Unknown Etiology ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Spot Virus ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Vidalia onion is an important crop in Georgia's agriculture with worldwide recognition as a specialty vegetable. Vidalia onions are shortday, Granex-type sweet onions grown within a specific area of southeastern Georgia. Tomato spotted wilt virus (TSWV) has been endemic to Georgia crops for the past decade, but has gone undetected in Vidalia onions. Tobacco thrips (Frankliniella fusca) and Western flower thrips (Frankliniella occidentalis) are the primary vectors for TSWV in this region, and a number of plant species serve as reproductive reservoirs for the vector or virus. Iris yellow spot virus (IYSV), an emerging tospovirus that is potentially a devastating pathogen of onion, has been reported in many locations in the western United States (2,4). Thrips tabaci is the known vector for IYSV, but it is unknown if noncrop plants play a role in its epidemiology in Georgia. During October 2003, a small (n = 12) sampling of onions with chlorosis and dieback of unknown etiology from the Vidalia region was screened for a variety of viruses, and TSWV and IYSV infections were serologically detected. Since that time, leaf and bulb tissues from 4,424 onion samples were screened for TSWV and IYSV using double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) with commercial kits (Agdia Inc., Elkhart, IN). Samples were collected from 53 locations in the Vidalia region during the growing season between November 2003 and March 2004. Plants exhibiting stress, such as tip dieback, necrotic lesions, chlorosis or environmental damage were selected. Of these, 306 were positive for TSWV and 396 were positive for IYSV using positive threshold absorbance of three times the average plus two standard deviations of healthy negative onion controls. Positive serological findings of the onion tissues were verified by immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR) for TSWV (3) and RT-PCR for IYSV (1). In both instances, a region of the viral nucleocapsid (N) gene was amplified. The PCR products were analyzed with gel electrophoresis with an ethidium bromide stain in 0.8% agarose. Eighty-six percent (n = 263) of the TSWV ELISA-positive samples exhibited the expected 774-bp product and 55 percent (n = 217) of the IYSV ELISA-positive samples exhibited the expected 962-bp product. The reduced success of the IYSV verification could be attributed to the age and deteriorated condition of the samples at the time of amplification. Thrips tabaci were obtained from onion seedbeds and cull piles within the early sampling (n = 84) and screened for TSWV by the use of an indirect-ELISA to the nonstructural (NSs) protein of TSWV. Of the thrips sampled, 25 were positive in ELISA. While the incidence of IYSV and TSWV in the Vidalia onion crop has been documented, more research is needed to illuminate their potential danger to Vidalia onions. References: (1) I. Cortês et al. Phytopathology 88:1276, 1998. (2) L. J. du Toit et al. Plant Dis. 88:222, 2004. (3) R. K. Jain et al. Plant Dis. 82:900, 1998. (4) J. W. Moyer et al. (Abstr.) Phytopathology 93(suppl.):S115, 2003.

scholarly journals First Report of Tomato spotted wilt virus on a New Host Leuzea carthamoides in Bulgaria and the World

Plant Disease ◽  
2013 ◽  
Vol 97 (9) ◽  
pp. 1258-1258 ◽  
Author(s):  
B. Dikova ◽  
N. Petrov ◽  
A. Djourmanski ◽  
H. Lambev
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Plant Viruses ◽  
Rt Pcr ◽  
First Report ◽  
Tomato Spotted Wilt ◽  
The World ◽  
Systemic Symptoms ◽  
Wilt Virus ◽  
The Town ◽  
Das Elisa

The Siberian plant Leuzea carthamoides or maral root was introduced to Europe as a medicinal crop. Tomato spotted wilt virus (TSWV), genus Tospovirus, family Bunyaviridae, caused a harmful outbreak on L. carthamoides in central Bulgaria near the town of Kazanluk in 2009. In 2011, TSWV was identified on young sprouts from the rootages of L. carthamoides in the same place near the town of Kazanluk, Bulgaria, by means of indicator (test) plants, double antibody sandwich (DAS)-ELISA, and reverse transcription (RT)-PCR. Disease symptoms were small yellow spots on the young leaves grown from the tested sprouts and distortions of the leaf lamina. The old leaves had large yellow spots and necrosis, without deformations. Most of those L. carthamoides plants with such symptoms died in the second and third year. The number of the plants in the plantations decreased 20 to 40% during the 3-year period and some of these losses were from the virus disease except the environment conditions. DAS-ELISA was carried out with polyclonal TSWV antiserum of LOEWE Biochemica, GmbH, Germany. We obtained positive extinction values ODλ 405nm 0.358 ± 0.091 compared to the negative 0.053 ± 0.016 and the positive control 0.510 at a confidential interval at P ≤ 0.05. TSWV symptoms were observed on the following indicator plants according to Antignus et al. (1) and DPV/412 (2): Chenopodium quinoa, Cucumis sativus, Datura stramonium, Nicotiana glutinosa, N. rustica, N. tabacum cv. Samsun NN, and Petunia hybrida. TSWV caused on C. quinoa and on cotyledons of C. sativus cv. Delikates local chlorotic lesions only. In this TSWV differed from CMV because CMV caused systemic mosaic symptoms. Local small necrotic lesions and no systemic symptoms were observed on P. hybrida. We noticed systemic symptoms caused from TSWV on D. stramonium, N. glutinosa, N. rustica, and N. tabacum cv. Samsun NN. The systemic symptoms were chlorotic spots, concentric ring spots, and line patterns proceeding to necrosis. RT-PCR, adapted by Mumford et al. (3), was carried out on samples of L. carthamoides. Oligonucleotide primer sequences were used in accordance with Mumford et al. (3). The DNA fragment was visualized by UV trans-illumination. A fragment of the TSWV genome with a length of 276 base pairs was found in three young L. carthamoides leaf samples taken from the sprouts (marker 100 bp). The PCR fragment was sequenced and deposited to NCBI with GenBank Accession No. KC918808. PCR master mix without RNA template was used as a negative control. L. carthamoides is a newly established TSWV host in the world. To our knowledge, this is the first report of TSWV in L. carthamoides identified by RT-PCR. References: (1) Y. Antignus et al. Phytoparasitica 25:319, 1997. (2) R. Kormelink. Descriptions of Plant Viruses, p. 412, 2005. (3) R. A. Mumford et al. J. Virol. Methods 57:109, 1996.

scholarly journals First Report of Tomato spotted wilt virus in Peppers and Tomato in the Dominican Republic

Plant Disease ◽  
2014 ◽  
Vol 98 (1) ◽  
pp. 163-163 ◽  
Author(s):  
R. T. Martínez ◽  
S. Poojari ◽  
S. A. Tolin ◽  
X. Cayetano ◽  
R. A. Naidu
Keyword(s):  
Dominican Republic ◽  
Tomato Spotted Wilt Virus ◽  
Caribbean Region ◽  
Rt Pcr ◽  
Spot Virus ◽  
Tomato Spotted Wilt ◽  
Fruit Samples ◽  
The Caribbean ◽  
Wilt Virus ◽  
Pepper Plants

In the Dominican Republic, green bell pepper (Capsicum annuum L.) and tomato (Solanum lycopersicum L.) are widely cultivated under protected greenhouse conditions as high value commercial crops for export. For the past 2 to 3 years, pepper and tomato have been observed in protected crop facilities in Jarabacoa and Constanza in the North Region with chlorotic and necrotic spots and rings on leaves, petioles, and stems, leaf bronzing, and tip necrosis. Fruits on symptomatic pepper and tomato plants showed concentric rings, irregular chlorotic blotches and deformation, and uneven maturation and development. Incidence on pepper and tomato was 20 to 100% and 5 to 20%, respectively. In initial tests, leaves and fruits from each of 20 symptomatic tomato and pepper plants from several greenhouse facilities were reactive in Tomato spotted wilt virus (TSWV; genus Tospovirus, family Bunyaviridae) immunostrip assays (Agdia, Inc., Elkhart, IN). Since these immunostrips are known to react with other tospoviruses, such as Tomato chlorotic spot virus (TCSV) and Groundnut ring spot virus, additional molecular diagnostic assays were conducted. Leaf and fruit samples from symptomatic plants were imprinted on nitrocellulose membrane (NCM) (2), air-dried, and sent to Washington State University for confirmatory tests. Viral nucleic acids were eluted from NCM discs (1) and subjected to reverse transcription (RT)-PCR using primers gL3637 (CCTTTAACAGTDGAAACAT) and gL4435 (CATDGCRCAAGARTGRTARACAGA) designed to amplify a portion of the L RNA segment of several tospoviruses (3). A single DNA product of ~800 bp was amplified from all samples. Amplicons from two tomato (leaf and fruit) and one pepper fruit samples were cloned separately into pCR2.1 (Invitrogen Corp., Carlsbad, CA). Two independent clones per amplicon were sequenced in both orientations. Sequence analyses of these clones (GenBank Accession Nos. KF 219673 to 75) showed 100% nucleotide sequence identity among themselves and 97% identity with corresponding L RNA sequences of pepper isolates of TSWV from Taiwan (HM180088) and South Korea (HM581940), 94 to 95% with tomato isolates of TSWV from South Korea (HM581934) and Hawaii (AY070218), and 89% with a tomato isolate from Indonesia (FJ177301). These results further confirm the presence of TSWV in symptomatic tomato and pepper plants. A comparison of TSWV sequences from the Dominican Republic with TSWV isolates from the United States and other countries in the Caribbean region could not be made due to the absence of corresponding sequences of the L-RNA of the virus from these countries in GenBank. TSWV-positive samples were negative for TCSV in RT-PCR, indicating the absence of this tospovirus that has been reported in the Caribbean region (data not shown). To our knowledge, this is the first confirmed report of TSWV in tomatoes and peppers in the Dominican Republic. The presence of vector thrips, Frankliniella occidentalis, on symptomatic plants was also confirmed, suggesting a role in the spread of TSWV under greenhouse conditions. Recent surveys identified some greenhouses with 100% symptomatic peppers. The presence of TSWV in tomato and pepper has important implications for the domestic and export vegetable industry in the Dominican Republic because of the broad host range of the virus (4). It is critical for commercial producers to monitor TSWV and deploy appropriate management strategies to limit virus spread. References: (1) O. J. Alabi et al. J. Virol. Methods 154:111, 2008. (2) P.-G. S. Chang et al. J. Virol. Methods 171:345, 2011. (3) F. H. Chu et al. Phytopathology 91:361, 2001. (4) G. Parrella et al. J. Plant Pathol. 85:227, 2003.

scholarly journals First Report of Pinaceae in Georgia Naturally Infected with Tomato spotted wilt virus

Plant Disease ◽  
2006 ◽  
Vol 90 (3) ◽  
pp. 376-376 ◽  
Author(s):  
S. W. Mullis ◽  
A. S. Csinos ◽  
R. D. Gitaitis ◽  
N. Martinez-Ochoa
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Pinus Elliottii ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Total Rna ◽  
Tomato Spotted Wilt ◽  
Pine Trees ◽  
Southern Georgia ◽  
Wilt Virus ◽  
Das Elisa

In October 2004, three pine tree seedlings included in an ongoing survey of annual weeds elicited positive reactions for Tomato spotted wilt virus (TSWV [family Bunyaviridae, genus Tospovirus]) using double assay sandwich-enzyme linked immunosorbent assay (DAS-ELISA) (Agdia Inc. Elkhart, IN). All the seedlings appeared healthy with no visible adverse effects from the virus. Over the next 12 months, an additional 1,326 samples of various pine species representing different growth stages were screened for TSWV. Samples were comprised of local populations of Pinus elliottii Engelm., P. taeda L., and P. palustris P. Mill., with the majority (n = 886) of samples being seedlings collected from southern Georgia. Along with the seedlings, needles, stem sections, and roots from saplings, as well as needles from mature trees, were screened for the virus. Of the trees sampled, 5.35% (n = 71) tested positive for TSWV, and of the seedlings 6.77% (n = 60) tested positive. The DAS-ELISA positive threshold was obtained using a figure of three times the average plus two standard deviations of healthy negative pine tissue control absorbance readings at 405 nm. A number of saplings testing positive (n = 6) were marked for further evaluation, and the needles from these saplings consistently screened positive for TSWV in subsequent testing. Furthermore, several samples were processed in modified burlese funnels to detect the possible presence of thrips. No thrips were ever identified in any of the burlese funnel collections. Different tissue types (needles, roots, stem sections, and reproductive organs) were screened, but the virus was only detected in needles. This suggests that local infections are only at feeding sites of viruliferous thrips. The known thrips vectors for TSWV are not considered to be pine feeders, and there is no indication that pine trees are a reproductive reservoir for any local thrips species. However, pine-feeding thrips may also feed on known weed hosts, thus pines could be a perennial reservoir. Mechanical inoculations from surface-sterilized infected pine needles onto known TSWV indicator plants (Nicotiana glutinosa L., N. benthamiana, and Emilia sonchifolia L. (DC)) were inconsistent. Successful transmission occurred 24% of the time. To further verify serological data, total RNA extracts of pine sap were purified and subjected to immunocapture-reverse transcriptase-polymerase chain reaction (IC-RT-PCR) using primers specific to the nucleocapsid gene of TSWV (1). IC-RT-PCR was used due to the inability to obtain useful total RNA from the pine tissues. This may be due to a secondary metabolite interfering with the total RNA extraction protocol. The IC-RT-PCR products were analyzed with electrophoresis using 0.01% ethidium bromide stain in a 0.8% agarose gel. Amplicons produced at the expected size (bp = 774) were considered positive for TSWV. Several were sequenced and were consistent with known, local TSWV isolates. There is no indication that TSWV is detrimental to pine trees, but considering the widespread distribution of the genus Pinus and the potential of serving as a reservoir of TSWV, it may play a role in the overall epidemiology of TSWV in southern Georgia. Reference: (1) R. K. Jain et al. Plant Dis. 82:900, 1998.

scholarly journals Infecção viral simples e dupla em Alstroemeria sp.

1999 ◽  
Vol 5 (1) ◽  
Author(s):  
Ligia M. L. Duarte ◽  
Patrícia V. Seabra ◽  
Eliana B. Rivas ◽  
Sílvia R. Galleti ◽  
Amélia V. Alexandre
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Tomato Spotted Wilt Virus ◽  
Sao Paulo ◽  
Tobacco Streak Virus ◽  
Streak Virus ◽  
São Paulo ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Amostras de <i>Alstroemeria sp</i>., provenientes de 2 regiões produtoras do Estado de São Paulo (Brasil) e da Itália (material quarentenado), foram submetidas a testes de transmissão mecânica, serológicos (DASELISA e decoração) e observações ao microscópio eletrônico. Das 33 amostras analisadas, 1 1 estavam infectadas por vírus, tendo sido identificados <i>Cucumber mosaic virus (CMV), Tobacco streak virus</i> (TSV) e <i>Tomato spotted wilt virus </i>(TSWV) tanto em infecções simples como mistas (TSV + CMV, TSV + TSWV e TSV + CMV + <i>Potyviridae</i>). Em 3 amostras infectadas foram observadas, ao microscópio eletrônico, partículas alongadas, porém não foi possível a identificação do vírus ao nível de espécie. Os dados obtidos mostram que a ocorrência de infecções mistas em Alstroemeria é um fato comum, envolvendo principalmente o TSV.

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