scholarly journals Effects of a highly concentrated platelet-rich plasma on the bone repair using non-critical defects in the calvaria of rabbits

2010 ◽  
Vol 25 (1) ◽  
pp. 28-33 ◽  
Author(s):  
Marco Antonio de Oliveira Filho ◽  
Paulo Afonso Nunes Nassif ◽  
Osvaldo Malafaia ◽  
Jurandir Marcondes Ribas Filho ◽  
Carmen Austrália Paredes Marcondes Ribas ◽  
...  
Keyword(s):  
Bone Repair ◽  
Platelet Rich Plasma ◽  
Blood Clot ◽  
Venous Blood ◽  
Control Group ◽  
Advanced Stage ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

PURPOSE: To verify the effect of highly concentrated platelet-rich plasma (hPRP) in the pathways of bone repair using non-critical defects in the calvaria of rabbits. METHODS: The hPRP was produced from collected venous blood of 21 rabbits. Four non-critical defects of 8 mm in diameter were created on the calvaria of each animal. The defects were all treated differently: autogenous particled bone (APB, group 1), autogenous particled bone associated with hPRP (APB + hPRP, group 2), isolated hPRP (group 3), and blood clot (control, group 4). Animals were submitted to euthanasia on the 2nd, 4th and 6th week postoperatively. Histological and histomorphometric analysis were carried through. RESULTS: After two weeks, groups 1 and 2 were in more advanced stage of repair than 3 and 4. At this period, comparing the groups 1 and 2, no significant differences were found between both, which also happened between the groups 3 and 4. However, after four and six weeks, the group 1 showed a more advanced stage of repair among all the other studied groups, while group 2 was in more advanced signs of bone repair than groups 3 and 4. Comparing groups 3 and 4, after four and six weeks, the least advanced stage of bone repair was found to be within group 3. CONCLUSION: The use of a highly concentrated PRP was considered prejudicial to the repair of non-critical defects in the calvaria of rabbits, either in the association of autogenous particled bone, when compared to autogenous particled bone alone, or in its isolated form, when compared to blood clot (control).

scholarly journals Antioxidant Effect of MnTE-2-PyP on Lung in Asthma Mice Model

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Lyudmil Terziev ◽  
Violeta Dancheva ◽  
Veneta Shopova ◽  
Galya Stavreva
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Control Group ◽  
Mice Model ◽  
Antioxidant Defence ◽  
Group 4 ◽  
Antioxidant Defence System ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Aim. To investigate the effects of MnTE-2-PyP on some markers of antioxidant defence system in asthma mice model.Material and Methods. The animals were divided into four groups: group 1, controls; group 2, injected with ovalbumin, group 3, treated with MnTE-2-PyP, and group 4, treated with ovalbumin and MnTE-2-PyP. The activities of superoxide dismutase, catalase, glutathione peroxidase and nonprotein sulfhydryl groups content (NPSH) were determined in lung homogenate.Results. The activities of superoxide dismutase and catalase in group 2 decreased significantly as compared to control group. The decrease of the same enzymes in group 4 was lower and significant as compared to group 2. Changes in the glutathione peroxidase activity showed a similar dynamics. The NPSH groups content decreased in group 2. In group 4 this decrease was relatively lower as compared to group 2.Conclusions. The application of MnTE-2-PyP mitigated the effects of oxidative stress in asthma mice model.

scholarly journals Pengaruh Monoklonal Antibodi Bovine Zona Pelusida 3 (bZP3) terhadap Diameter dan Atresia Folikel Ovarium Mencit (Mus musculus)

2018 ◽  
Vol 24 (1) ◽  
pp. 37
Author(s):  
Annisa Trissatharra ◽  
Sri Ratna Dwiningsih ◽  
Ratna Sofaria Munir
Keyword(s):  
Mus Musculus ◽  
Ovarian Follicles ◽  
Observation Time ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Objectives: To identify the effect of monoclonal antibody bZP3 at ovarian follicles that undergo atresia and diameter of various ovarian follicles.Materials and Methods: This is a true experimental research with post only control group design. Samples were 36 female mices (Mus musculus) which is divided into 6 groups, there are 3 control groups (group 1, 2, and 3) injected by Phospatase Buffer Saline (PBS) 50µl and 3 treatment groups (group 4, 5, and 6) injected by Mab bZP3 50µl. Group 1 and 4 terminated at 5th day, group 2 and 5 terminated at 10th day, and group 3 and 6 terminated at 20th day. Evaluation of atretic ovarian follicles and diameter of ovarian follicles performed by hematoxylin eosin (HE) and the data processed by parametric statistic.Results: There are no significant in different among groups in the aspect of atretic follicles and diameter of folicles (p>0.05), but descriptively, number of follicles undergo atresia of the follicle primary, secondary, and tertiary treatment group was higher than the control group, except on the 20th day of observation time.Conclusion: administration of Mab bZP3 had no effect to amount of atretic follicles and diameter of folicles during observation time.

scholarly journals Zingerone Ameliorates Hepatotoxicity

2020 ◽  
Vol 11 (1) ◽  
pp. 280-284 ◽  
Author(s):  
Julietpoornamathy J ◽  
Parameswari C.S.
Keyword(s):  
The Body ◽  
Control Group ◽  
Medical Sciences ◽  
Toxic Compounds ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Insight Into ◽  
Group 1

In medical sciences, toxicity is an area wherein extensive studies have been carried to improve the diseases as well as to prevent. So, there is a high requirement for novel and improved alternative therapeutic strategies to manage diseases. The liver is the largest gland in the body, which executes several important mechanisms; it stores minerals and vitamins and releases them in periods of need. The main aim of this study was to give a closer insight into potent non- toxic compounds that is capable of modifying the responses. Animals were divided into five equal groups viz control (Group 1), administered with food and water ad libitum, (Group 2) administered with olive oil, (Group 3) administered with zingerone, (Group 4) administered with concanavalin A, (Group 5) administered with cyclosporine A followed by zingerone. Our results revealed significant changes in liver marker enzymes and liver histology of zingerone treated rats when compared to control rats.  A corollary, zingerone has no toxic effect on hepatocytes and was found to be safe at a dose of 10mg/kg b wt and also ameliorates hepatotoxicity.

Double activation improves rabbit freeze–thawed oocytes developmental potential

Zygote ◽  
2009 ◽  
Vol 18 (1) ◽  
pp. 27-32 ◽  
Author(s):  
J. Wang ◽  
L. Cong ◽  
Z.G. Zhang ◽  
Y.X. Cao ◽  
Z.L. Wei ◽  
...  
Keyword(s):  
Control Group ◽  
Strontium Chloride ◽  
Random Group ◽  
Developmental Potential ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Double Activation ◽  
Potential Methods ◽  
Group 1

SummaryObjective: To investigate the effects of various activation methods on freeze–thawed rabbit oocytes developmental potential. Methods: Rabbit oocytes were vitrified by cryoleafs and cryoprotected with ethylene glycol and propanediol. After thawing, the oocytes were fertilized by intracytoplasmic sperm injection (ICSI). Surviving oocytes after ICSI were divided into five groups at random. Group 1: Oocytes (n = 30) activated 1 h after ICSI by calcium ionomycin (I0634); Group 2: Oocytes (n = 26) activated by strontium chloride an hour after ICSI; Group 3: Oocytes (n = 33) activated by I0634 twice; Group 4: Oocytes (n = 28) were activated by strontium chloride twice; Control Group: Inactivated oocytes (n = 39). Blastocysts derived from each group were transplanted to recipient rabbits. Results: Rates of fertilization, cleavage and blastocyst formation of Group 3 were higher than those of Group 1 and Group 2 (81.8% vs 33.3% vs 53.8%, 54.5% vs 16.7% vs 26.9%, p < 0.05; 15.2% vs 3.3% vs 7.7%, p > 0.05). The rabbit transplanted with embryos derived from Group 3 became pregnant. Embryos derived from double activation could implant into endometrium. Conclusion: Double activation may increase freeze–thawed oocytes developmental potential. After activation, oocytes cleavage velocity may be faster than that of oocytes without activation.

scholarly journals Efficacy of 2 Different Intratympanic Steroid Regimen on Prevention of Cisplatin Ototoxicity: An Experimental Study

2019 ◽  
pp. 014556131987431
Author(s):  
Burak Mustafa Taş ◽  
Gökçe Şimşek ◽  
Musa Azman ◽  
Rahmi Kılıç
Keyword(s):  
Otoacoustic Emission ◽  
Auditory Brainstem ◽  
Organ Injury ◽  
Auditory Brainstem Responses ◽  
Control Group ◽  
Protective Effects ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Ototoxicity is the general name of cochlear and vestibular organ injury resulting from encountering various therapeutic agents and chemical substances. Cisplatin is commonly used in the treatment of many cancers. In this study, the efficacy of intratympanic steroids was compared for preventing cisplatin ototoxicity. In this study, 32 (64 ears) rats were used by separating into 4 groups. Cisplatin was administered intraperitoneally to the first group (n = 8). Methylprednisolone and then cisplatin were administered intratympanically to the second group (n = 8). On the third group (n = 8), dexamethasone and then cisplatin were administered intratympanically. To the fourth group (n = 8), 0.9% NaCl and then cisplatin were given intratympanically. Otoacoustic emission (OAE) measurements and auditory brainstem responses (ABRs) tests were performed on all groups before and 72 hours after the procedure. Pretreatment of ABR-IV values were 4.29 ± 0.19 milliseconds in group 2 and 4.27 ± 0.16 milliseconds in group 3, whereas posttreatment ABR-IV values were 4.95 ± 0.35 milliseconds in group 2 and 4.65 ± 0.26 milliseconds in group 3. The ABR-IV values were measured significantly shorter in the rats given dexamethasone and methylprednisolone, according to control and cisplatin groups ( P < .001). Pretreatment of ABR I-IV interval values were 2.98 ± 0.34 milliseconds and 3.03 ± 0.42 milliseconds in group 1 and group 4, respectively, and ABR I-IV interval values in group 1 and group 4 posttreatment were 3.49 ± 0.39 milliseconds and 3.5 ± 0.39 milliseconds in group 1 and group 4, respectively. Auditory brainstem responses I-IV interval was significantly longer in the cisplatin and control group than in the rats given dexamethasone and methylprednisolone ( P < .001). After cisplatin treatment, OAE amplitudes decreased significantly in group 1 and group 4 for all frequencies, while OAE values were protected in methylprednisolone and dexamethasone group ( P < .001). In conclusion, it has been shown that both agents have protective effects on cisplatin ototoxicity, with dexamethasone slightly more than methylprednisolone.

Effects of Dietary α-Tocopherol and Polyunsaturated Fats on the Fatty Acid Composition of Platelet Phospholipids and on Blood Coagulation

1975 ◽  
Author(s):  
K. Korsan-Bengtsen ◽  
B. Hallgren ◽  
A.-C Teger-Nilsson
Keyword(s):  
Fatty Acid ◽  
Negative Correlation ◽  
Platelet Rich Plasma ◽  
Alpha Tocopherol ◽  
Control Group ◽  
Platelet Factor ◽  
Group 4 ◽  
Polyunsaturated Fats ◽  
Group 2 ◽  
Group 1

The study group was 40 male post myocardial infarction patients 47-57 years old. All the participants were investigated two times with two weeks interval after which they were randomly divided into four groups with 10 subjects in each. Group 1 was given alpha-tocopherol 300 mg/day, group 2 was given alpha tocopherol 300 mg/day and a diet containing extra polyunsaturated fats, group 3 was given extra polyunsaturated fats but no extra alpha-tocopherol and group 4 served as a control group – thus continued their ordinary diet. After three months all participants were again investigated twice with two weeks interval.On the values from all 40 subjects before the start of the dietary regimens linear regression analyses showed that there was a significant correlation between the content of the fatty acid 18 : 0 in the serin cephalin fraction and re calcification time in platelet rich plasma (RPRP), and a negative correlation between 20: 4 and RPRP. There was also a correlation between the ratio 18: 0/20: 4 and RPRP and a negative correlation between 18: 0/20: 4 and platelet factor 3 activity in plasma.In group 2 there was a significant decrease in 18:0 and an increase in 20: 4 in the serin cephalin fraction from platelets after the diet period compared to preexperimental values. Russel’s viper venom clotting time (RVV) decreased significantly in group 1. There was a significant correlation between the decrease in RVV and the increase in plasma alphatocopherol.

scholarly journals Inhibition of Corneal Neovascularization by Topical and Subconjunctival Tigecycline

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Sertan Goktas ◽  
Ender Erdogan ◽  
Rabia Sakarya ◽  
Yasar Sakarya ◽  
Mustafa Yılmaz ◽  
...  
Keyword(s):  
Topical Administration ◽  
Corneal Neovascularization ◽  
Control Group ◽  
Therapeutic Effects ◽  
Control Groups ◽  
Group 4 ◽  
Percentage Area ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Objective. To investigate the effects of topical and subconjunctival tigecycline on the prevention of corneal neovascularization.Materials and Methods. Following chemical burn, thirty-two rats were treated daily with topical instillation of 1 mg/mL tigecycline (group 1) or subconjunctival instillation of 1 mg/mL tigecycline (group 3) for 7 days. Control rats received topical (group 2) or subconjunctival (group 4) 0.9% saline. Digital photographs of the cornea were taken on the eighth day after treatment and analyzed to determine the percentage area of the cornea covered by neovascularization. Corneal sections were analyzed histopathologically.Results. The median percentages of corneal neovascularization in groups 1 and 3 were 48% (95% confidence interval (CI), 44.2–55.8%) and 33.5% (95% CI, 26.6–39.2%), respectively. The median percentages of corneal neovascularization of groups 1 and 3 were significantly lower than that of the control group (P=0.03andP<0.001, resp.). Histologic examination of samples from groups 1 and 3 showed lower vascularity than that of control groups.Conclusion. Topical and subconjunctival administration of tigecycline seems to be showing promising therapeutic effects on the prevention of corneal neovascularization. Furthermore, subconjunctival administration of tigecycline is more potent than topical administration in the inhibition of corneal neovascularization.

scholarly journals The Effects of Bile Duct Obstruction on Liver Volume: An Experimental Study

ISRN Surgery ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Bahtiyar Ertor ◽  
Serdar Topaloglu ◽  
Adnan Calik ◽  
Umit Cobanoglu ◽  
Ali Ahmetoglu ◽  
...  
Keyword(s):  
Bile Duct ◽  
Obstructive Jaundice ◽  
Liver Volume ◽  
Control Group ◽  
Group 4 ◽  
Duct Ligation ◽  
Group 3 ◽  
Baseline Values ◽  
Group 2 ◽  
Group 1

Objectives. This study is aimed at investigating alterations in liver volume during obstructive jaundice in rat liver. Materials and Methods. Thirty-six rats were divided into four groups. Abdominal tomography was performed for baseline volumetric analyses. The main bile ducts were ligated (BDL). Volumetric analyses were repeated 3 days after BDL in group 1, 7 days after BDL in group 2, 15 days after BDL in group 3, and 25 days after BDL in group 4, and total hepatectomy was performed in all animals. Control group () was created with the rats that died before bile duct ligation. Results. There was no difference found in liver volume in group 1 compared to control animals. The liver volume was increased 7 days after BDL (). It was increased up to 60% of baseline values 25 days after BDL (). Wet liver weights of animals were also increased compared to control group. Liver weights were increased up to 40% percent of baseline values in group 4 (). Conclusions. Liver volume and weight were increased after BDL. Liver surgery in patients with huge liver mass is generally associated with significant difficulty. The surgeon should be aware of the time-dependent alteration in liver volume after obstructive jaundice.

scholarly journals The Effects of Insufflation Conditions on Rat Mesothelium

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Andrew K. Davey ◽  
Jessica Hayward ◽  
Jean K. Marshall ◽  
Anthony E. Woods
Keyword(s):  
Inflammatory Response ◽  
Mesothelial Cell ◽  
Surface Group ◽  
Control Group ◽  
Cell Nuclei ◽  
Tissue Samples ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Aim. The aim of this investigation was to examine the alterations in the peritoneum after cold dry CO2, heated dry CO2, and humidified heated CO2at pressures equivalent to intraperitoneal pressures used in human laparoscopy.Methods. Eighteen rats were divided into 4 treatment groups—group 1: untreated control; group 2: insufflation with cold dry CO2; group 3: insufflation with heated, dry CO2; group 4: insufflation with heated and humidified CO2. The abdomen was insufflated to 5 mm/Hg (flow rate 50 mL/min) for 2 h. Twelve hours later, tissue samples were collected for analysis by light microscopy (LM) and scanning electron microscopy (SEM).Results. Group 1: no abnormalities were detected. Group 2: specimens revealed an inflammatory response with loss of mesothelium and mesothelial cell nuclei showing lytic change. Cells were rounded with some areas of cell flattening and separation. Group 3: some animals showed little or no alteration, while others had a mild inflammatory response. Mesothelial cells were rounded and showed crenation on the exposed surface. Group 4: specimens showed little change from the control group.Conclusions. The LM results indicate that insufflations with heated, humidified CO2are the least likely to induce mesothelial damage.

Clove (Syzygium aromaticum) Effect on Carbon Tetrachloride-Induced Rat: Comparison of Malondialdehyde Level of Liver and Blood Plasma

2018 ◽  
Vol 24 (8) ◽  
pp. 6053-6057
Author(s):  
Muhammad K Azwar ◽  
Ani R Prijanti
Keyword(s):  
Carbon Tetrachloride ◽  
Blood Plasma ◽  
Antioxidant Properties ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Syzygium Aromaticum ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Studies suggested antioxidant properties of the content of Syzygium aromaticum (clove). The study was conducted to obtain better understanding about the effect of clove on concentration of oxidative stress marker malondialdehyde (MDA) in liver and blood plasma of rat initially induced by carbon tetrachloride (CCl4); and whether blood plasma MDA level might represent liver condition. Experimental research was done using 20 Wistar rats classified into 5 treatment groups: (1) CCl4 - and clove-positive treatment after 3 days of clove treatment, (2) one day after, (3) alpha-tocopherol as positive control, (4) CCl4 only as negative control, and (5) normal control. Wills method was used for MDA concentration measurement. Liver MDA concentration were 0.0262 ± 0.0010 for group 1, 0.0214 ± 0.0047 group 2, 0 for group 3, 0.0077 ± 0.0094 group 4, and 0.0039 ± 0.0009 control group in nmol/mg protein (p = 0.001), whereas in the blood plasma it was 29.6032 ± 6.8021 for group 1, 26.1103 ± 3.6920 for group 2, 1.1612 ± 0.3555 for group 3, 1.4585 ± 1.4747 for group 4, and 2.4217 ± 1.2382 control group in nmol/mL (p = 0.001). Contrary to study in the past, no antioxidant properties were observed in treatment with dose 200 mg clove/kg body weight of rat. Such treatment increased MDA concentration and enhanced CCl4-induced damage in a time-dependent fashion. Strong correlation between MDA concentration in the liver and blood plasma (R = 0.97; p = 0.003) suggested blood plasma utilisation to represent hepatic MDA concentration or damage.

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