scholarly journals The male reproductive system and the effect of an extract of a medicinal plant (Hypericum perforatum) on the labeling process of blood constituents with technetium-99m

2007 ◽  
Vol 50 (spe) ◽  
pp. 97-104 ◽  
Author(s):  
Sebastião David Santos-Filho ◽  
Adenilson de Souza da Fonseca ◽  
Mario Bernardo-Filho
Keyword(s):  
Reproductive Performance ◽  
Reproductive System ◽  
Hypericum Perforatum ◽  
Vas Deferens ◽  
Stannous Chloride ◽  
Male Reproductive System ◽  
Human Consumption ◽  
Blood Constituents ◽  
Technetium 99M ◽  
Young Rats

Hypericum perforatum (hiperico) is a plant that has been used to treat diseases and also inhibits rat and human vas deferens contractility. In nuclear medicine, stannous chloride (SnCl2) is used as a reducing agent to obtain radiopharmaceuticals labeling with technetium-99m. As the SnCl2 seems to have adverse effects related with the reproductive performance of male rabbits as well as the human consumption of hiperico might affect sexual function. In the present work, consistent results show significant changes on the blood constituents labeled by technetium-99m obtained from young rats under the effect of an hiperico extract as opposed to blood samples equally treated taken from elderly rat.. Supposedly, this extract could protect the male reproductive system against action of SnCl2 at least in young rats. The findings described in this work allow introducing a simple assay to evaluate the action of products that could interfere with the male reproductive system.

scholarly journals Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m

2008 ◽  
Vol 51 (spe) ◽  
pp. 151-155 ◽  
Author(s):  
Silvana Ramos Farias Moreno ◽  
Jorge José de Carvalho ◽  
Ana Lúcia Nascimento ◽  
Beni Olej ◽  
Emely Kazan Rocha ◽  
...  
Keyword(s):  
Stannous Chloride ◽  
Cellular Proteins ◽  
Uncaria Tomentosa ◽  
Blood Constituents ◽  
In Vitro Methods ◽  
Technetium 99M ◽  
In Vivo Labeling ◽  
In Vitro Treatment

The influence (in vivo and in vitro) of an Uncaria tomentosa extract (Cats claw) on the labeling of red blood cells (RBCs) and plasma and cellular proteins with technetium-99m (Tc-99m) was evaluated. For the in vivo treatment, animals were treated with Cats claw. For the in vitro treatment, heparinized blood was incubated with Cats claw before the addition of stannous chloride (SnCl2) and Tc-99m. Samples of plasma (P) and RBCs were separated and also precipitated with trichloroacetic acid. The soluble and insoluble fractions of P and RBCs were isolated. The analysis of the results of the in vivo study, indicates that there is no significant alteration on the uptake of Tc-99m by the blood constituents, but it significantly decrease (p<0.05) the labeling of blood constituents by in vitro methods. These effects could be due to chelation of stannous and /or pertechnetate ions and blockage of the Tc-99m bindings sites.

Ultrastructure of the male reproductive system of the free-living marine nematode Paracyatholaimus pugettensis Wieser & Hopper, 1967 (Chromadorida: Cyatholaimidae)

Nematology ◽  
2010 ◽  
Vol 12 (2) ◽  
pp. 255-268 ◽  
Author(s):  
Julia K. Zograf
Keyword(s):  
Epithelial Cells ◽  
Seminal Vesicle ◽  
Reproductive System ◽  
Vas Deferens ◽  
Proximal Part ◽  
Male Reproductive System ◽  
Synthetic Activity ◽  
The Sea Of Japan ◽  
Marine Nematode ◽  
Free Living

AbstractAlthough nematodes are a well studied group of multicellular organisms, until now the only information on the cellular structure of the male reproductive system of marine nematodes is that on the histology of free-living marine nematode from the order Enoplida. The fine structure of the male reproductive system of the free-living marine nematode Paracyatholaimus pugettensis (Chromadorida: Cyatholaimidae) from the Sea of Japan has been studied using TEM. The testis epithelium has a large distal tip cell similar to that described for representatives of the subclass Rhabditia. The epithelial wall of the testis is differentiated along its length. The proximal part of the epithelial tube consists of relatively large cells bearing numerous surface outgrowths that permeate between the developing spermatocytes. The epithelium in the middle region of the testis is formed from extremely flattened cells. The distal part of the testis – the seminal vesicle – is filled with immature spermatozoa and consists of absorptive cells. The seminal vesicle is followed by the vas deferens. The gonoduct is also differentiated along its length, the first third being formed from synthetically active epithelial cells, the two layers of which form a tiled structure. There is no lumen in the gonoduct and it is probable that, due to the tiled structure, the epithelial cells move apart to create space for the spermatozoa during ejaculation. The posterior two-thirds of the duct is surrounded by muscle cells that create the necessary pressure during ejaculation. The enlarged epithelial cells of the vas deferens show vigorous synthetic activity, which is probably involved in the transformation of immature spermatozoa into mature gametes.

scholarly journals Expression of estrogen receptor-alpha and -beta mRNAs in the male reproductive system of the rat as revealed by in situ hybridization

2001 ◽  
Vol 26 (3) ◽  
pp. 165-174 ◽  
Author(s):  
CN Mowa ◽  
T Iwanaga
Keyword(s):  
Estrogen Receptor ◽  
In Situ Hybridization ◽  
Reproductive System ◽  
Initial Segment ◽  
Vas Deferens ◽  
Perinatal Period ◽  
Reproductive Organs ◽  
Male Reproductive System ◽  
Prenatal Period

We mapped the cellular expression of estrogen receptor (ER) alpha and ERbeta mRNAs in the male reproductive system of the rat during development and adulthood by in situ hybridization. The expression patterns of ERalpha mRNA in the gonad, efferent duct and initial segment of the epididymis during the perinatal period were essentially similar to those of the adult: ERalpha mRNA signals were expressed most intensely in the epithelia of the efferent ducts and initial segment of the epididymis, and in the interstitial cells of the testis from the prenatal period to adulthood. However, ERalpha mRNA signals in the primordial epididymis and vas deferens during the prenatal period were confined to the outermost cellular layer of the ducts, whereas thereafter they were only expressed weakly in the epithelium and stroma of the epididymis and moderately in the muscle layer of the vas deferens. ERbeta signals were expressed intensely (1) in primordial germ and Sertoli cells only during the prenatal period, (2) in arterial walls in the adult testis, and (3) in the epithelium of the sex accessory glands from the perinatal period to adulthood. This report is the first to describe the cellular distribution of ER mRNA in the male reproductive organs during the perinatal period, and complements and confirms earlier data on its distribution in the adult. The broad expression of ERs in male reproductive organs suggests roles for estrogen in regulating tissue development and reproductive events.

scholarly journals An aqueous extract of Vitex agnus castus alters the labeling of blood constituents with technetium-99m

2007 ◽  
Vol 50 (spe) ◽  
pp. 183-188 ◽  
Author(s):  
Maria Regina de Macedo Costa ◽  
Camila Godinho Ribeiro ◽  
Sebastião David Santos-Filho ◽  
Rosane de Figueiredo Neves ◽  
Adenilson de Souza da Fonseca ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Plasma Proteins ◽  
Stannous Chloride ◽  
Basic Research ◽  
Chemical Compounds ◽  
Blood Constituents ◽  
Popular Medicine ◽  
Technetium 99M ◽  
Agnus Castus ◽  
Stannous Ion

The development of experimental assays to study properties of herbal medicine is worthwhile. Vitex agnus castus (VAC) is utilized in popular medicine and some actions have been attributed to its extract. Blood cells (BC) and plasma proteins are labeled with technetium-99m (Tc-99m) and have been used in nuclear medicine, as in basic research. This procedure uses a reducing agent and stannous ion is utilized. There are reports that drugs can alter this labeling process. The aim of this work was to evaluate the influence of an aqueous extract of VAC on the labeling of blood constituents with Tc-99m. Blood was incubated with VAC, stannous chloride and Tc-99m, as sodium pertechnetate, and centrifuged. Samples of BC and plasma were separated, aliquots of BC and plasma were also precipitated with trichloroacetic acid to obtain soluble and insoluble fractions and the percentage of radioactivity (%ATI) was determined. The results show a statistical (p<0.05) alteration in the %ATI on blood compartments and on the insoluble fractions of plasma and BC. Probably, this extract would have chemical compounds with oxidant properties.

scholarly journals Histology and Histometric Anatomy of the Male Reproductive System of Bat (Eidolon helvum)

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
A. Danmaigoro ◽  
J. E. Onu ◽  
M. L. Sonfada ◽  
M. A. Umaru ◽  
F. O. Oyelowo
Keyword(s):  
Reproductive System ◽  
Vas Deferens ◽  
Prostate Gland ◽  
Male Reproductive System ◽  
Seminiferous Tubules ◽  
Luminal Diameter ◽  
Fruit Bat ◽  
Mucosal Lining ◽  
Eidolon Helvum ◽  
Insight Into

The male reproductive system of fruit bat (Eidolon helvum) was studied histologically using light microscope. Thirty males (17 adults and 13 juveniles) were captured using net, weighed, aged using relative ossification of the wing bone, and dissected and reproductive tissue was processed for histomorphometry. On the basis of histological sections, the structures of a pair of testis containing the seminiferous tubules of adults were compacted in organization with spermatogenic cells. The epididymis has a thinner muscular region than the vas deferens with longitudinal folds on the mucosal lining. Two portions were observed in the prostate gland, while seminal vesicle has numerous trabeculae and bulbourethral gland was observed to have multiacini. There was increase in thickness of muscular region, epithelial height, and luminal diameter of epididymis and vas deferens between adults and juveniles. This work has documented the histology of the male reproductive system in bats, and ultrastructure and histochemistry are recommended for further insight into the reproductive biology.

Transmission and scanning electron microscopy of the male reproductive system of Schistosoma margrebowiei Le Roux, 1933

1989 ◽  
Vol 63 (3) ◽  
pp. 197-205 ◽  
Author(s):  
A. H. H. Awad ◽  
A. J. Probert
Keyword(s):  
Electron Microscopy ◽  
Reproductive System ◽  
Vas Deferens ◽  
Large Mass ◽  
Male Reproductive System ◽  
Apical Region ◽  
Axial Filament ◽  
Dense Bodies ◽  
Transmission Electron ◽  
Germinal Cells

ABSTRACTThe structure of the male reproductive system of Schistosoma margrebowiei as viewed by light, scanning and transmission electron microscopy is described. The cirrus tube opens at the genital pore without the presence of a cirrus and is spongy and leaf-like. The structure of the germinal and non-germinal cells of the testes are described. The spermatozoon consists of a head, without an acrosome, and a flagellum with a 9+0 arrangement of microtubles in the axial filament. A large mass of mitochondria is situated in the anterior end of the head and cortically arranged longitudinally running microtubles are present. The apical region of the epithelial cells of the vas deferens extends into the lumen of the duct forming convoluted lamellae. This arrangement, together with the presence of dense bodies within the cells, suggests that these cells have a secretory function.

The vasa deferentia of the male reproductive system of Calpodes ethlius (Hesperiidae, Lepidoptera)

1982 ◽  
Vol 60 (6) ◽  
pp. 1172-1183 ◽  
Author(s):  
J. Lai-Fook
Keyword(s):  
Seminal Vesicle ◽  
Reproductive System ◽  
Vas Deferens ◽  
Male Reproductive System ◽  
Secretory Epithelium

The vasa deferentia of Calpodes ethlius are distinguishable into upper and lower regions, each of which consists of expanded and narrower tubular portions. The expanded region of the upper vas deferens is lined by a phagocytic epithelium while the narrower region is lined by a granular secretory epithelium. The expanded region of the lower vas deferens is a true seminal vesicle since it is always full of sperm bundles; it is lined by a nongranular secretory epithelium which extends to the junction with the duplex ejaculatoris. Distinctly different cells occur at the junctions of the three epithelia with each other. The entire vas deferens is surrounded by one or two thin muscle layers.

scholarly journals The effect of an extract from Ganoderma lucidum (reishi) on the labeling of blood constituents with technetium-99m and on the survival of Escherichia coli

2008 ◽  
Vol 51 (spe) ◽  
pp. 157-162 ◽  
Author(s):  
Raquel Terra Agostinho ◽  
Sebastião David Santos-Filho ◽  
Adenilson de Souza da Fonseca ◽  
Sotiris Missailidis ◽  
Mario Bernardo-Filho
Keyword(s):  
Escherichia Coli ◽  
Blood Cells ◽  
Ganoderma Lucidum ◽  
Wistar Rats ◽  
Stannous Chloride ◽  
Blood Samples ◽  
Blood Constituents ◽  
Technetium 99M ◽  
Bacterial Cultures

This study evaluated effects of an aqueous extract of Ganoderma lucidum (reishi) on the labeling of blood constituents with technetium-99m (99mTc) and on the survival of cultures of Escherichia coli treated with stannous chloride. Blood samples from Wistar rats were treated with reishi extract, radiolabeling procedure was performed, plasma (P), blood cells (BC) and insoluble (IF) and soluble (SF) fractions of P and BC were separated. The radioactivity was counted for the determination of the percentages of radioactivity (%ATI). Cultures of Escherichia coli AB1157 were treated with stannous chloride in the presence and absence of reishi extract. Blood samples and bacterial cultures treated with NaCl 0.9% were used as controls. Data indicated that reishi extract altered significantly (p<0.05) the %ATI of P, BC, IF-P, SF-P, IF-BC and SF-BC, as well as increased the survival of bacterial cultures treated with stannous chloride. Our results suggest that reishi extract could present a redox/chelating action, altering the labeling of blood constituents with 99mTc and protecting bacterial cultures against oxidative damage induced by stannous chloride.

scholarly journals Morphology and histology of the male reproductive system of Collaria oleosa (Distant, 1883) (Heteroptera: Miridae)

Bionatura ◽  
2020 ◽  
Vol 5 (2) ◽  
pp. 1122-1125
Author(s):  
Mayra Vélez ◽  
Glenda Dias ◽  
Irina Morales ◽  
Raul N. C. Guedes ◽  
José Lino-Neto
Keyword(s):  
Reproductive System ◽  
Vas Deferens ◽  
Seminal Vesicles ◽  
Male Reproductive System ◽  
General Morphology ◽  
Accessory Glands ◽  
New Information ◽  
Ejaculatory Bulb

Collaria oleosa (Distant, 1883) is a phytophagous bug often observed causing injuries in wheat, barley, and oat crops, besides grass pastures. The male reproductive system of C. oleosa has been studied anatomically and histologically. It consists of a pair of testes, each one with two follicles, one pair of seminal vesicles, two pairs of external accessory glands, and an ejaculatory bulb. The testicular follicles exhibit cysts with spermatogonia, spermatocytes, spermatids, and spermatozoa bundles. The testes are connected to the seminal vesicles by the vas deferens. The seminal vesicles are connected to the ejaculatory bulb and are bound by the accessory glands. In mirids belonging to the Stenodemini tribe, the general morphology of the male reproductive system is similar. However, two follicles per testis may be characteristic of the genus Collaria. The anatomy and histology of the male reproductive system in C. oleosa provides new information and also contributes to the understanding of the systematics of Stenodemini as well as the Miridae family in general.

scholarly journals Effects of fenoprofen on the labeling of blood constituents with technetium-99m, the morphology of red blood cells and the plasmid

2008 ◽  
Vol 51 (spe) ◽  
pp. 135-141 ◽  
Author(s):  
Marcia de Oliveira Pereira ◽  
Gabrielle de Souza Rocha ◽  
Simone dos Santos Lombardi ◽  
Mauro Geller ◽  
Mário José Pereira ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Stannous Chloride ◽  
Quantitative Morphology ◽  
Blood Constituents ◽  
Qualitative And Quantitative ◽  
Technetium 99M ◽  
Rbc Membrane ◽  
Blood Smears ◽  
Chloride Effect

The aim of this work was to evaluate the effect of fenoprofen on the labeling of blood constituents with technetium-99m, on the morphology of red blood cells and on the plasmid DNA. Blood samples from Wistar rats were incubated with fenoprofen and the assay of labeling of blood constituents with technetium-99m (99mTc) was performed. Blood cells, plasma, soluble and insoluble fractions of blood cells and plasma were separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) was determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the red blood cells (RBC) was evaluated. Plasmid (pBSK) was incubated with fenoprofen with stannous chloride, and agarose gel electrophoresis procedure was carried out to evaluate genotoxic and the protection of this drug against stannous chloride effect on DNA. In conclusion, under the conditions used in this work, our data suggest that fenoprofen would not (i) affect the fixation of the 99mTc on the blood constituents, (ii) alter the RBC membrane and (iii) present genotoxic and redox effects.

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