scholarly journals Physiological performance and expression of isozymes in maize seeds subjected to water stress

2014 ◽  
Vol 36 (1) ◽  
pp. 40-47 ◽  
Author(s):  
Viviane Maria de Abreu ◽  
Édila Vilela de Resende Von Pinho ◽  
Renzo Garcia Von Pinho ◽  
Glória Maria de Freitas Naves ◽  
Izabel Costa Silva Neta ◽  
...  
Keyword(s):  
Water Stress ◽  
Maize Cultivars ◽  
Maize Seeds ◽  
Test Number ◽  
Enzymatic Systems ◽  
Physiological Quality ◽  
Osmotic Potentials ◽  
Selection Of

Early characterization of genotypes, through the previous assessment of physiological quality of their seeds and their enzymatic systems, can provide parameters capable of aiding in the selection of new maize cultivars tolerant to water stress. Thus, this study was aimed at assessing seed performance of five different maize lines subjected to four different water stress levels. The physiological quality of seeds germinated under the osmotic potentials of 0.0, -0.3, -0.6 and -0.9 MPa was assessed by germination test, number of strong normal seedlings and T50, as well as by the expression of the isozymes superoxide dismutase (SOD), catalase (CAT), glutamate oxaloacetate transaminase (GOT), esterase (EST), malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), and of heat-resistant proteins. The osmotic potential of -0.9 MPa affected the physiological quality and vigor of seeds of all maize lines assessed. The highest values for the germination percent, in the lowest osmotic potentials assessed, were found in the seeds of line 63. However, seeds of line 91 were superior in regard to vigor. Increased expression of the enzymes SOD and CAT, considered as enzymes of the antioxidant system, was observed in seeds of lines 44 and 91. Thus, line 91 was considered as promising for tolerance to water stress.

scholarly journals Germination of cotton cultivar seeds under water stress induced by polyethyleneglycol-6000

2011 ◽  
Vol 68 (2) ◽  
pp. 131-138 ◽  
Author(s):  
Carlos Henrique Salvino Gadelha Meneses ◽  
Riselane de Lucena Alcântara Bruno ◽  
Pedro Dantas Fernandes ◽  
Walter Esfrain Pereira ◽  
Leonardo Henrique Guedes de Morais Lima ◽  
...  
Keyword(s):  
Water Stress ◽  
Seed Viability ◽  
Accelerated Aging ◽  
Germination Percentage ◽  
Randomized Design ◽  
Physiological Quality ◽  
Differential Viability ◽  
Osmotic Potentials ◽  
Cotton Cultivar

The physiological quality of cotton cultivar seeds (Gossypium hirsutum var. latifolium L.) was evaluated in laboratory by the simulation of water potentials with polyethyleneglycol-6000 (0.0; -0.2; -0.4; -0.6; -0.8 and -1.0 MPa), at 25ºC using germitest paper as substrate. A completely randomized design in a 4 × 6 factorial scheme with four replications of 50 seeds each was used. The studied variables were: germination percentage, first count of germination, germination velocity index, accelerated aging in water, electrical conductivity, humidity, vigor classification, radicle length and radicle/shoot length ratio. The effect of water stress on seed viability and on plantlet vigor was severe at potentials below -0.4 MPa. The 'CNPA 187 8H' cultivar was the least sensitive to the tested osmotic potentials, both in terms of germination and of vigor. The 'BRS-201' cultivar was mostly affected by the viability and vigor tests under water deficit conditions. Differential viability and vigor between cultivars were observed under the water stress levels.

scholarly journals Physiological quality of seed and seedling performance of crambe genotypes under water stress

2017 ◽  
Vol 21 (3) ◽  
pp. 175-179 ◽  
Author(s):  
Fernando H. B. Machado ◽  
Andréia M. S. de S. David ◽  
Lucas V. S. Cangussú ◽  
Josiane C. Figueiredo ◽  
Hugo T. R. Amaro
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Randomized Design ◽  
Physiological Quality ◽  
Completely Randomized Design ◽  
And Performance ◽  
Osmotic Agents ◽  
Polyethylene Glycol 6000 ◽  
Osmotic Potentials

ABSTRACT Water stress is a condition that causes physiological changes in different species and even genotypes of the same species. One of the osmotic agents most used to simulate this condition is polyethylene glycol 6000. The aim of this study was to evaluate the effects of water stress on the physiological quality of seeds and performance of seedlings of crambe genotypes. A completely randomized design was used, in a factorial scheme with two genotypes (one cultivar and one line) and five osmotic potentials simulated with aqueous solutions of polyethylene glycol 6000 (control = 0, -0.25, -0.50, -1.0, -1.50 MPa), with four replicates of 50 seeds per treatment. The effects of the treatments were evaluated by means of germination, first count, germination speed index and fresh and dry matter of seedlings. The physiological quality of seeds and the performance of crambe seedlings are negatively affected under water stress from -0.25 MPa on. Seeds of the genotype FMSCR 1101 have greater tolerance to drought stress, regardless of the osmotic potential used.

scholarly journals Physiological quality of hybrid maize seeds through respiratory and enzymatic activities

2016 ◽  
Vol 11 (20) ◽  
pp. 1879-1886 ◽  
Author(s):  
Oliveira dos Santos Heloisa ◽  
Vilela Von Pinho Iolanda ◽  
Vilela de Resende Von Pinho Eacute dila ◽  
Maria de Oliveira Pires Raquel ◽  
Fabiana da Silva Valk iacute ria ◽  
...  
Keyword(s):  
Enzymatic Activities ◽  
Maize Seeds ◽  
Physiological Quality ◽  
Hybrid Maize

scholarly journals Priming and drying on the physiological quality of eggplant seeds

2012 ◽  
Vol 30 (3) ◽  
pp. 484-488 ◽  
Author(s):  
Delineide P Gomes ◽  
Alexandre F da Silva ◽  
Denise Cunha FS Dias ◽  
Eveline M Alvarenga ◽  
Laércio J da Silva ◽  
...  
Keyword(s):  
Root Length ◽  
Primary Root ◽  
Dry Weight ◽  
Control Group ◽  
Peg 6000 ◽  
Physiological Quality ◽  
Three Sheets ◽  
Osmotic Potentials ◽  
Primary Root Length

We evaluated the effect of the priming with polyethyleneglicol (PEG) 6000 followed or not of drying on two lots of eggplant seeds with medium and low vigor respectively. The seeds were submitted to the treatments of priming with PEG 6000 in the osmotic potentials of -0.6, -0.9 and -1.2 MPa during 48 or 96 hours of priming with and without subsequent drying. For the soaking of seeds in osmotic solutions, these were placed over three sheets of paper boxes such germitest gerbox conditioning moistened with each solution in a 3:1 ratio. The control group was represented by unprimed seeds. The priming for both lots provided higher values in relation to the vigor through the first counting of germination, seedlings and primary root length, germination and emergence rates, emergence and dry weight of seedlings when compared to the non-primed seeds. These results were not verified for germination; significant differences practically were not observed in relation to the controls. Improvements in relation to the vigour were not higher on lot 2 than those obtained on lot 1. The priming with PEG 6000 with subsequent drying increased the vigor of eggplant seeds of the cultivar Morro Grande in relation to those non-primed, however, without differences in the germination.

scholarly journals Physiological quality of maize seeds produced under soil water deficit conditions

2020 ◽  
Vol 24 (7) ◽  
pp. 451-456
Author(s):  
Fernando H. B. Machado ◽  
Andréia M. S. de S. David ◽  
Silvânio R. dos Santos ◽  
Josiane C. Figueiredo ◽  
Cleisson D. da Silva ◽  
...  
Keyword(s):  
Soil Water ◽  
Water Deficit ◽  
Irrigation Water ◽  
Field Capacity ◽  
Soil Water Deficit ◽  
Maize Hybrids ◽  
Maize Seeds ◽  
Physiological Quality ◽  
Water Depths

ABSTRACT Crop strategies focused on the rational use of water are required in semiarid regions. Thus, the objective of this work was to evaluate the physiological quality of maize seeds produced under soil water deficit conditions. Five irrigation water depth were established, based on the field capacity (100, 85, 70, 55 and 40%), to control the available water for two maize hybrids (2B-587 and DKB-390). A randomized block design was used, with a split-plot arrangement and four repetitions. The plots consisted of irrigation water depths, and the subplots consisted of maize hybrids. The seed water concentration, germination, and vigor were determined after the harvest, using data from first germination counting, seedling emergence, germination speed index, seedling length, and accelerated aging, electrical conductivity, and modified cold tests. The maize hybrid DKB-390 showed better physiological potential under the soil water deficit conditions evaluated. The irrigation water depths lower than 70% of field capacity resulted in decreases in soil water contents and affected negatively the physiological quality of the maize seeds produced.

scholarly journals Physiological quality of osmoprimed gherkin seeds

2013 ◽  
Vol 35 (3) ◽  
pp. 368-373 ◽  
Author(s):  
Rodrigo de Góes Esperon Reis ◽  
Humberto Pereira da Silva ◽  
José Maria Gomes Neves ◽  
Renato Mendes Guimarães
Keyword(s):  
Electrical Conductivity ◽  
Room Temperature ◽  
Dry Weight ◽  
Potassium Nitrate ◽  
Germination Percentage ◽  
Initial Profile ◽  
Randomized Design ◽  
Physiological Quality ◽  
Osmotic Potentials

It was aimed to verify the effect of different methodologies of osmopriming on physiological quality of gherkin seeds. Prior, it has been characterized the initial profile of the gherkin seeds. Then, the seeds were osmoprimed in gerboxes containing two blotter papers wetted with osmotic solutions in a volume equal to three times the paper dry weight and kept in BOD at 15 °C. After the priming, the seeds were dried at room temperature for 48 hours. Then, the following variables were evaluated: seeds moisture content, percentage of germination, percentage of emergence, emergence speed index and electrical conductivity. The analyses of variances were realized according to a randomized design in a factorial scheme 3 x 2 x 4: three solutes (polyetilene glycol 6000 - PEG, potassium nitrate - KNO3 and PEG + KNO3), two osmotic potentials (-0.55 and -1.10 MPa) and four times of priming (24, 48, 72 and 96 hours), followed by analyses of regression. It is concluded that the priming has no effect on germination and affects positively the vigor of the gherkin seeds lots; osmopriming with potassium nitrate is effective in improving the physiological quality of gherkin seeds lot.

scholarly journals Ampelographic and oenological characterization of the ancient grapevine cv. Dobričić grown in the coastal region of Croatia

OENO One ◽  
2012 ◽  
Vol 46 (4) ◽  
pp. 263
Author(s):  
Goran Zdunić ◽  
Irena Budić-Leto ◽  
Urska Vrhovsek ◽  
Iva Tomić-Potrebuješ ◽  
Edi Maletić
Keyword(s):  
Coastal Region ◽  
Red Wines ◽  
High Quality ◽  
Virus Incidence ◽  
High Virus ◽  
First Time ◽  
Outstanding Quality ◽  
Selection Of

<p style="text-align: justify;"><strong>Aims</strong>:A four-year study (2007 through 2010) was carried out to determine agronomic, biological, and oenological characteristics of Dobričić grapevines so as to evaluate this cultivar and protect it from extinction.</p><p style="text-align: justify;"><strong>Methods and results</strong>: 38 characteristics from the OIV descriptor list were used to describe young shoot, mature leaf, cluster, and berry morphology of Dobričić grapevines. The dimensions of an average leaf were constructed from measured leaf variables. A typical Dobričić genotype at nine microsatellite loci is presented. ELISA analysis revealed high virus incidence and all samples tested were GLRaV-3 positive. The profile of different classes of polyphenols in Dobričić wine was determined using HPLC and spectrophotometry. The concentrations of polyphenols in Dobričić wine, especially anthocyanins, hydroxycinnamic acids and stilbenes, were high compared to major red wines reported in the literature. Descriptive sensory analysis was carried out to determine the aroma attributes describing a Dobričić monovarietal wine.</p><p style="text-align: justify;"><strong>Conclusion</strong>: Knowledge of the biodiversity of the grapevine cultivars of Croatia is still scant. This study provides for the first time ampelographic and oenological information on Dobričić grapevines and demonstrates its high quality potential.</p><p style="text-align: justify;"><strong>Significance and impact of the study</strong>: The results of the ampelographic characterization would be helpful in the identification and selection of Dobričić for cultivation in certain vine-growing areas. The outstanding quality of Dobričić makes it very promising for wine blending (colour improvement) and plant breeding purposes.</p>

scholarly journals Physiological quality of parsley seeds in function of size

2021 ◽  
Vol 42 (3Supl1) ◽  
pp. 1499-1510
Author(s):  
Renato Téo de Barros ◽  
◽  
Cibele Chalita Martins ◽  
Tatiane Sanches Jeromini ◽  
Givanildo Zildo da Silva ◽  
...  
Keyword(s):  
Seed Size ◽  
Dry Weight ◽  
Germination Percentage ◽  
Randomized Design ◽  
Physiological Quality ◽  
Completely Randomized Design ◽  
Germination Vigor ◽  
Selection Of

Parsley seeds are commercialized without classification by size during processing, unlike other crops. This classification procedure allows the standardization of seed size for high precision in sowing, disposal of unwanted material, and selection of high quality seeds. The aim of this study was to verify the effect of seed size on germination, vigor, and yield performance of two parsley cultivars. Seeds from eight lots of parsley of ‘Portuguesa’ and ‘Chacareira’ cultivar were separated into three size classes, represented by the seeds retained in the sieves of 0.84, 1.00 and 1.41mm mesh opening. After classification, seeds were evaluated in the laboratory for water content, germination percentage, first count, and thousand seed weight. In the field, the fresh and dry weight of aerial part and the final number of plants (stand) were evaluated after 78 days of sowing. The experimental design was the completely randomized design in an 8 x 3 factorial scheme (eight lots and three sieve opening) with four replicates, for both cultivars. Data obtained were subjected to analysis of variance and means were compared using Tukey test at 5% probability level. It was concluded that the parsley seeds separated by the 1.41 mm sieves showed higher vigor, and consequently, greater success in stand establishment at the field than those separated by other sieve size. The use of sieves in the classification of parsley seeds helps in obtaining lots with superior physiological quality.

scholarly journals Effect of processing stages in the physiological quality of maize seeds

2016 ◽  
Vol 10 (6) ◽  
pp. 819-823
Author(s):  
Thiago Alberto Ortiz ◽  
◽  
Marcio Antônio Nicoletti ◽  
Lúcia Sadayo Assari Takahashi ◽  
◽  
...  
Keyword(s):  
Maize Seeds ◽  
Physiological Quality

scholarly journals Biochemical profiling and its role in physiological quality of maize seeds

2018 ◽  
Vol 40 (1) ◽  
pp. 7-15 ◽  
Author(s):  
Daniele Nerling ◽  
Cileide Maria Medeiros Coelho ◽  
Adriele Brümmer
Keyword(s):  
Soluble Sugars ◽  
Accelerated Aging ◽  
Inorganic Phosphorus ◽  
Inbred Lines ◽  
Total Protein Content ◽  
Breeding Programs ◽  
Maize Hybrids ◽  
Maize Seeds ◽  
Physiological Quality

Abstract: Viability and vigor of seeds are physiological attributes influenced by a myriad of factors including the genotype. Biochemical profiling of maize seeds and its correlation with their physiological quality is of crucial importance in breeding programs that the aim is seeds of better quality. The main goal of this study was to analyze the genetic diversity among maize inbred lines and evaluate the biochemical components that have an important role in physiological quality of seeds. The seeds of the inbred lines and hybrids were submitted to viability test, vigor by the accelerated aging and cold tolerance, and biochemical profiling (total protein content, soluble proteins, total phosphorus, phytate, inorganic phosphorus, starch and soluble sugars). The genetic divergence between the inbred lines found, prompt us to conclude that genetic gains may exist in association with physiological quality of maize hybrids. Seeds with higher inorganic phosphorus and soluble sugars presented superior physiological quality than seeds with lower levels of those compounds.

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