THE LOCALIZATION OF [3H]ALDOSTERONE AND [3H]CORTISOL WITHIN RENAL TUBULAR CELLS BY ELECTRON MICROSCOPE AUTORADIOGRAPHY

1967 ◽  
Vol 39 (4) ◽  
pp. 543-NP ◽  
Author(s):  
M. A. WILLIAMS ◽  
W. I. BABA
Keyword(s):  
Electron Microscope ◽  
Plasma Membranes ◽  
Kidney Cells ◽  
Electron Microscope Autoradiography ◽  
Tubular Cells ◽  
Renal Tubular Cells ◽  
Microscope Autoradiography ◽  
Renal Tubular ◽  
Convoluted Tubules

SUMMARY Microgram quantities of tritiated aldosterone or cortisol were injected into the aorta of rats. The kidneys were removed at various times after injection, and examined by light and electron microscope autoradiography. The mineralocorticoids or their metabolites were bound to kidney cells in amounts sufficient to give autoradiographs. Radioactivity occurred mainly in the proximal and distal convoluted tubules. Within these cells it was bound mainly to mitochondria and plasma membranes. The results are discussed in relation to the current theories of mineralocorticoid action.

Effect of triethyltin on Ca2+ movement in Madin–Darby canine kidney cells

2002 ◽  
Vol 21 (8) ◽  
pp. 457-462 ◽  
Author(s):  
B-P Jiann ◽  
K-J Chou ◽  
H-T Chang ◽  
W-C Chen ◽  
J-K Huang ◽  
...  
Keyword(s):  
Mdck Cells ◽  
Kidney Cells ◽  
Madin Darby Canine Kidney ◽  
Independent Manner ◽  
Tubular Cells ◽  
Renal Tubular Cells ◽  
Inositol 1,4,5 Trisphosphate ◽  
Renal Tubular ◽  
Darby Canine Kidney ◽  
Canine Kidney

The effects of the environmental toxicant, triethyltin, on Ca2 + mobilization in Madin–Darby canine kidney (MDCK) cells have been examined. Triethyltin induced an increase in cytosolic free Ca2 + levels ([Ca2 +]i) at concentrations larger than 2 mM in a concentrationdependent manner. Within 5 min, the [Ca2 +]i signal was composed of a gradual rise and a sustained phase. The [Ca2 +]i signal was partly reduced by removing extracellular Ca2 +. In Ca2 +-free medium, pretreatment with thapsigargin (1 mM), an endoplasmic reticulum Ca2 + pump inhibitor, reduced 50 mM triethyltin-induced [Ca2 +]i increase by 80%. Conversely, pretreatment with triethyltin abolished thapsigargin-induced Ca2 + release. Pretreatment with U73122 (2 mM) to inhibit phospholipase C-coupled inositol 1,4,5-trisphosphate formations failed to alter 50 mM triethyltin-induced Ca2 + release. Incubation with triethyltin at a concentration (1 mM) that did not increase basal [Ca2 +]i for 3 min did not alter ATP (10 mM)and bradykinin (1 mM)-induced [Ca2 +]i increases. Collectively, this study shows that triethyltin altered Ca2 + movement in renal tubular cells by releasing Ca2 + from multiple stores in an inositol 1,4,5-trisphosphate-independent manner, and by inducing Ca2 + influx.

Incorporation of Cholesterol into Peripheral Nerve Myelin

1972 ◽  
Vol 30 ◽  
pp. 334-335
Author(s):  
Frank A. Rawlins
Keyword(s):  
Electron Microscope ◽  
Sciatic Nerve ◽  
Electron Microscope Autoradiography ◽  
Myelin Sheaths ◽  
Microscope Autoradiography ◽  
Grain Distribution ◽  
Sciatic Nerves ◽  
Autoradiographic Analysis ◽  
Old Mice ◽  
Short Times

Several speculations exist as to the site of incorporation of preformed molecules into myelin. The possibility that an autoradiographic analysis of cholesterol-1,2-H3 incorporation at very short times after injection might shed some light in the solution of that problem led to the present experiment.Cholesterol-1,2-H3 was injected intraperitoneally into 24 tenday old mice. The animals were then sacrificed at 10,20,30,40,60,90,120 and 180 min after the injection and the sciatic nerves were processed for electron microscope autoradiography. To analyze the grain distribution in the autoradiograms of cross and longitudinal sections from each sciatic nerve myelin sheaths were subdivided into three compartments named: outer 1/3, middle 1/3 and inner 1/3 compartments.It was found that twenty min. after the injection of cholesterol -1.2-H3 (Figs. 1 and 2), 55% of the total number of grains (t.n.g) found in myelin were within the outer 1/3 compartment, 9% were within the middle 1/3 and 36% within the inner 1/3 compartment

scholarly journals Human antigen R regulates hypoxia‐induced mitophagy in renal tubular cells through PARKIN/BNIP3L expressions

2021 ◽  
Author(s):  
Shao‐Hua Yu ◽  
Kalaiselvi Palanisamy ◽  
Kuo‐Ting Sun ◽  
Xin Li ◽  
Yao‐Ming Wang ◽  
...  
Keyword(s):  
Tubular Cells ◽  
Human Antigen R ◽  
Renal Tubular Cells ◽  
Renal Tubular

scholarly journals Interleukin-22 attenuates renal tubular cells inflammation and fibrosis induced by TGF-β1 through Notch1 signaling pathway

Renal Failure ◽  
2020 ◽  
Vol 42 (1) ◽  
pp. 381-390 ◽  
Author(s):  
Rong Tang ◽  
Xiangcheng Xiao ◽  
Yang Lu ◽  
Huihui Li ◽  
Qiaoling Zhou ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Tubular Cells ◽  
Interleukin 22 ◽  
Renal Tubular Cells ◽  
Notch1 Signaling ◽  
Renal Tubular ◽  
Notch1 Signaling Pathway ◽  
Tgf Β1

scholarly journals Hepatocyte Nuclear Factor-1β Controls Mitochondrial Respiration in Renal Tubular Cells

2017 ◽  
Vol 28 (11) ◽  
pp. 3205-3217 ◽  
Author(s):  
Audrey Casemayou ◽  
Audren Fournel ◽  
Alessia Bagattin ◽  
Joost Schanstra ◽  
Julie Belliere ◽  
...  
Keyword(s):  
Mitochondrial Respiration ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Tubular Cells ◽  
Renal Tubular Cells ◽  
Renal Tubular
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