Increased natriuretic peptides in fetal hearts of diabetic rats

1995 ◽  
Vol 146 (2) ◽  
pp. 255-259 ◽  
Author(s):  
S Mulay ◽  
P R Conliffe ◽  
D R Varma
Keyword(s):  
Amniotic Fluid ◽  
Natriuretic Peptides ◽  
Fetal Heart ◽  
Myocardial Hypertrophy ◽  
Maternal Diabetes ◽  
Diabetic Rats ◽  
Reverse Phase ◽  
Fetal Plasma ◽  
Amniotic Fluid Volume ◽  
Cardiac Natriuretic Peptides

Abstract The main purpose of these studies was to determine whether diabetic pregnancy altered maternal and fetal atrial natriuretic peptide (ANP). Diabetes was induced in rats by intravenous injection of 40 mg streptozotocin/kg on day 2 of gestation. Immunoreactive ANP in plasma, amniotic fluid and hearts on day 20 of gestation was measured by radioimmunoassay; fetal cardiac natriuretic peptides (ANP, proANP and BNP) were separated by reverse-phase high pressure liquid chromatography. Diabetes caused an increase in fetal plasma insulin, placental weight, amniotic fluid volume, the ratio of the fetal heart to body weight, maternal and fetal plasma ANP, fetal cardiac ANP and fetal cardiac BNP. It is suggested that the maternal diabetes-induced increase in fetal ANP might be related to fetal myocardial hypertrophy and could contribute to hydramnios. Journal of Endocrinology (1995) 146, 255–259

Function curve of the membranes that regulate amniotic fluid volume in sheep

2005 ◽  
Vol 289 (1) ◽  
pp. H146-H150 ◽  
Author(s):  
Job Faber ◽  
Debra Anderson ◽  
Roger Hohimer ◽  
Qin Yang ◽  
George Giraud ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Absorption Rate ◽  
Plasma Renin ◽  
Fluid Volume ◽  
Natural Rate ◽  
Fetal Plasma ◽  
Amniotic Fluid Volume ◽  
Urine Production ◽  
Rate Of Absorption ◽  
Vascular Catheters

Seven singleton 120-day fetal lambs were prepared with a shunt from the lung to the gastric end of the esophagus, a bladder catheter, and multiple amniotic fluid and vascular catheters. The urachus was ligated. Beginning 7 days later, amniotic fluid volumes were determined by drainage, followed by replacement with 1 liter of lactated Ringer (LR) solution. Urine flow into the amnion was measured continuously. In 14 of 27 experiments, amniotic fluid volumes were determined again 2 days after the inflow into the amnion had consisted of urine only and in 13 experiments after the inflow of urine had been supplemented by an intraamniotic infusion of LR solution. Intramembranous absorption was calculated from the inflows and the changes in volume between the beginning and end of each experiment. The relations between absorption rate and amniotic fluid volume, the “function curves,” were highly individual. Urine production during the infusion of LR solution did not decrease, fetal plasma renin activity decreased ( P < 0.001), and amniotic fluid volume increased by 140% [SE (27%), P < 0.005], but the increase in the amniochorionic absorption rate of 411% [SE (48%), P < 0.001] was greater ( P < 0.005) than the increase in volume. Each of the seven fetuses was proven capable of an average intramembranous absorption rate that exceeded 4.5 liters of amniotic fluid per day. During the infusion of LR solution, the increase in the rate of absorption matched the rate of infusion (both in ml/h), with a regression coefficient of 0.75 ( P < 0.001). Thus, even for large amniotic fluid volumes, volume is not limited by the absorptive capacity of the amniochorion, and, at least in these preparations, the position of the function curve and not the natural rate of inflow was the major determinant of resting amniotic fluid volume.

Ovine fetal adaptations to chronically reduced urine flow: preservation of amniotic fluid volume

1996 ◽  
Vol 81 (6) ◽  
pp. 2588-2594 ◽  
Author(s):  
Stephanie E. Mann ◽  
Mark J. M. Nijland ◽  
Michael G. Ross
Keyword(s):  
Amniotic Fluid ◽  
Plasma Osmolality ◽  
Urine Osmolality ◽  
Urine Flow ◽  
Fluid Volume ◽  
Fetal Plasma ◽  
Arterial Blood ◽  
Amniotic Fluid Volume ◽  
Fetal Urine ◽  
Ovine Fetal

Mann, Stephanie E., Mark J. M. Nijland, and Michael G. Ross.Ovine fetal adaptations to chronically reduced urine flow: preservation of amniotic fluid volume. J. Appl. Physiol. 81(6): 2588–2594, 1996.—Adequate amniotic fluid (AF) volume is maintained by a balance of fetal fluid production (lung liquid and urine) and resorption (swallowing and intramembranous flow). Because fetal urine is the principle source of AF, alterations in urine flow and composition directly impact AF dynamics. Intra-amniotic 1-desamino-8-d-arginine vasopressin (DDAVP) is rapidly absorbed into fetal plasma and induces a marked fetal urinary antidiuresis. To examine the effect of intra-amniotic- DDAVP-induced fetal urinary responses on AF volume and composition, six chronically prepared ewes with singleton fetuses (gestation 128 ± 2 days) were studied for 72 h after a single intra-amniotic DDAVP (50-μg) injection. After DDAVP, fetal urine osmolality significantly increased at 2 h (157 ± 13 to 253 ± 21 mosmol/kg) and remained elevated at 72 h (400 ± 13 mosmol/kg). Urinary sodium (33.0 ± 4.5 to 117.2 ± 9.7 meq/l) and chloride (26.0 ± 2.8 to 92.4 ± 8.1 meq/l) concentrations similarly increased. AF osmolality increased (285 ± 3 to 299 ± 4 mosmol/kgH2O), although there was no change in fetal plasma osmolality (294 ± 2 mosmol/kg). Despite a 50% reduction in fetal urine flow (0.12 ± 0.03 to 0.05 ± 0.02 ml ⋅ kg−1 ⋅ min−1at 2 h and 0.06 ± 0.01 ml ⋅ kg−1 ⋅ min−1after 72 h), AF volume did not change (693 ± 226 to 679 ± 214 ml). There were no changes in fetal arterial blood pressures, pH,[Formula: see text], or[Formula: see text] after DDAVP. We conclude the following. 1) Intra-amniotic DDAVP injection induces a prolonged decrease in fetal urine flow and increases in urine and AF osmolalities. 2) Despite decreased urine flow, AF volume does not change. We speculate that, in response to DDAVP-induced fetal oliguria, reversed intramembranous flow (from isotonic fetal plasma to hypertonic AF) preserves AF volume.

Intrapartum Doppler velocimetry, amniotic fluid volume, and fetal heart rate as predictors of subsequent fetal distress

1989 ◽  
Vol 161 (6) ◽  
pp. 1508-1514 ◽  
Author(s):  
Albert P. Sarno ◽  
Myoung Ock Ahn ◽  
Harbinder S. Brar ◽  
Jeffrey P. Phelan ◽  
Lawrence D. Platt
Keyword(s):  
Heart Rate ◽  
Amniotic Fluid ◽  
Fetal Heart Rate ◽  
Fetal Heart ◽  
Fetal Distress ◽  
Fluid Volume ◽  
Doppler Velocimetry ◽  
Amniotic Fluid Volume

scholarly journals Obstetric outcome study of oligohydramnios beyond 34 completed weeks of gestation

2018 ◽  
Vol 7 (9) ◽  
pp. 3857
Author(s):  
Hema K. R. ◽  
Lalitha H. S.
Keyword(s):  
Amniotic Fluid ◽  
Fetal Heart ◽  
Close Association ◽  
Outlet Obstruction ◽  
Fetal Distress ◽  
Caesarian Section ◽  
Fluid Volume ◽  
Research Centre ◽  
Amniotic Fluid Index ◽  
Amniotic Fluid Volume

Background: Perinatal morbidity and mortality are significantly increased when oligohydramnios is present. As the amniotic fluid volume decreases, the perinatal mortality rate increases. The incidence of major congenital anomalies with IUGR also increases as the amniotic fluid volume declines. There is a close association between declining placental function in the latter part of the third trimester and amniotic fluid volume1. Thus, post term patients are at a greater risk for development of oligohydramnios. Fetal anomalies that results in oligohydramnios classically involve the urinary tract. The most frequently mentioned renal anomalies include bilateral renal agenesis, multicystic dysplastic kidneys, bladder outlet obstruction and infantile polycystic kidneys.Methods: All antenatal patients seen in the Department of Obstetrics and Gynecology at Sri Siddhartha Medical College and Research Centre between January 2015 to January 2017, who were willing to participate in the trial study were enrolled. Ultrasound GE Voulson S8-PRO equipped with 3.5 MHz transducer was used to measure the amniotic fluid index. The AFI in each group was noted and the pregnancy outcome in each group was determined. At the time of delivery following data variables were collected and compiled.Results: There were 105 women, who were divided into three groups of 35 each. Those women with AFI<5 had more chances of emergency caesarian section, the causes were fetal distress with variable and late fetal heart decelerations. The incidence of meconium stained liquor and NICU admission were more.Conclusions: The women with AFI<5 had more chances of emergency caesarian section, the causes were fetal distress with variable and late fetal heart decelerations. Amniotic Fluid Index is a valuable screening test for detecting fetuses that may have poor perinatal outcome.

Effects of insulin, glucose and ACTH on corticosterone production by fetal adrenal cells from diabetic rats

1989 ◽  
Vol 120 (3) ◽  
pp. 393-399 ◽  
Author(s):  
P. Conliffe ◽  
S. Mulay
Keyword(s):  
Blood Glucose ◽  
Direct Action ◽  
Plasma Concentrations ◽  
Maternal Diabetes ◽  
Diabetic Rats ◽  
Thymidine Uptake ◽  
Sprague Dawley ◽  
Adrenal Cells ◽  
Fetal Plasma ◽  
Severe Diabetes

ABSTRACT Experiments were carried out on Sprague–Dawley rats to determine whether changes in fetal corticosterone levels during maternal diabetes were caused by the accompanying fetal hyperinsulinaemia or fetal hyperglycaemia. Diabetes was induced by injecting streptozotocin (30–45 mg/kg, i.v.) on day 2 of gestation. Fetal adrenals were removed on day 20 of gestation and cultured. Streptozotocin caused moderate (blood glucose 14–22·5 mmol/l) to severe (blood glucose >25 mmol/l) diabetes. Both moderate and severe diabetes caused a decrease in fetal body weights. Relative to non-diabetic controls, maternal and fetal plasma concentrations of corticosterone were higher in the severely and lower in the moderately diabetic rats. Corticosterone production by fetal adrenal cells from control and moderately diabetic rats was comparable, but cells from the severely diabetic animals produced significantly greater amounts of corticosterone than did control cells. Neither glucose (28 mmol/l) nor insulin (1 nmol/l) exerted significant effects on [3H]thymidine uptake or corticosterone production by fetal adrenal cells from non-diabetic, moderately diabetic or severely diabetic rats. Human ACTH (0·02–20 nmol/l) caused a concentration-dependent increase in corticosterone output of comparable magnitude by cells from all three groups of animals. These data suggest that fetal growth abnormalities during diabetic pregnancy are not directly related to changes in glucocorticoid levels and that changes in glucocorticoid levels are not caused by any direct action of fetal hyperinsulinaemia or hyperglycaemia on adrenal cells. Journal of Endocrinology (1989) 120, 393–399

Sonographic Assessment of Amniotic Fluid Volume using Single Deepest Pocket (SDP) among Igbo Women with Uncomplicated Singleton Pregnancies.

2019 ◽  
Vol 2 (1) ◽  
pp. 1-18
Author(s):  
Chijioke Okeudo ◽  
B.U. Ezem
Keyword(s):  
Amniotic Fluid ◽  
Gestational Age ◽  
Cross Sectional Study ◽  
Fluid Volume ◽  
Singleton Pregnancy ◽  
Cross Sectional ◽  
South Eastern ◽  
Amniotic Fluid Volume ◽  
The Mean ◽  
Singleton Pregnancies

Background: The amniotic fluid is fundamental for proper fetal development and growth. Ultrasound visualization of the amniotic fluid permits both subjective and objective estimates of the amniotic fluid. Objective: The objective of this study was to determine the reference values of normal single deepest pocket (SDP) – upper and lower limits, mean SDP and variation of the SDP with gestational age among Igbo women of South-Eastern Nigeria extraction carrying uncomplicated singleton pregnancy. Methodology: This was a prospective cross sectional study involving 400 women carrying uncomplicated singleton pregnancies and who were sure of the date of the first day of their last menstrual period. The single deepest pocket / maximum vertical pool were determined once at presentation at the hospital.. The study was conducted from January 1st to December 31st 2015. The second author carried out all the scanning. The SDP was obtained. Results: The womens’ mean and median ages were the same at 28 years. The gestational age range of the pregnancies was 14-41 weeks. The mean SDP was 5.8cm, while the 5th and 95th percentiles were 3.3cm and 8.5cm respectively. There was no difference in the mean SDP in both term and preterm. There was irregular but continuous rise of mean SDP to a peak of 6.8cm at gestational age of 39 weeks. In conclusion, the participants had a mean SDP of 5.8cm. There was also a positive correlation between SDP and Gestational age. We therefore recommend a longitudinal study to assess perinatal outcome and abnormal amniotic fluid volume among Igbo women of South-Eastern Nigeria. Key words: Single Deepest Pocket, Uncomplicated Singleton Pregnancy, Igbo Women.

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