scholarly journals Polymorphism identification in goat DGAT1 and STAT5A genes and association with milk production traits

2013 ◽  
Vol 58 (No. 7) ◽  
pp. 321-327 ◽  
Author(s):  
X.P. An ◽  
J.X. Hou ◽  
H.B. Zhao ◽  
L. Bai ◽  
J.Y. Peng ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Milk Production ◽  
Milk Yield ◽  
Milk Fat ◽  
Production Traits ◽  
Milk Production Traits ◽  
Dgat1 Gene ◽  
Pcr Rflp ◽  
Stat5a Gene ◽  
T Locus

Polymorphisms of DGAT1 and STAT5A genes in Xinong Saanen and Guanzhong goat breeds were investigated. PCR-RFLP, SSCP, and DNA sequencing were used to identify three SNPs: DQ380250:g.407_408insC in the DGAT1 gene, AJ237937:g.6798C&gt;T and g.6852C&gt;T in the STAT5A gene. In DGAT1 g.407_408insC locus, the frequencies of C<sup>&ndash;</sup> allele were 0.79&ndash;0.85, and frequencies of C<sup>+</sup> allele were 0.21&ndash;0.15. At STAT5A g.6852C&gt;T locus, frequencies of C allele were 0.70&ndash;0.72, and frequencies of T allele were 0.30&ndash;0.28. Compared with goats with DGAT1 C<sup>&ndash;</sup>C<sup>&ndash;</sup>, those with C<sup>&ndash;</sup>C<sup>+</sup> genotype had greater milk fat (P &lt; 0.05). The goats with STAT5A CT had greater milk yield than those with CC genotype (P &lt; 0.05). The results showed that does with C<sup>&ndash;</sup>C<sup>&ndash;</sup>CT and C<sup>&ndash;</sup>C<sup>+</sup>CT yielded more milk than those with C<sup>&ndash;</sup>C<sup>&ndash;</sup>CC(P &lt; 0.05). In addition, does with C<sup>&ndash;</sup>C<sup>+</sup>CT had the highest milk fat in comparison with other combination genotypes (P &lt; 0.05). &nbsp;

An association analysis between PRL genotype and milk production traits in Italian Mediterranean river buffalo

2017 ◽  
Vol 84 (4) ◽  
pp. 430-433 ◽  
Author(s):  
Jun Li ◽  
Aixin Liang ◽  
Zipeng Li ◽  
Chao Du ◽  
Guohua Hua ◽  
...  
Keyword(s):  
Milk Production ◽  
Milk Yield ◽  
Milk Fat ◽  
Fat Content ◽  
Phenotypic Variance ◽  
River Buffalo ◽  
Production Traits ◽  
Milk Production Traits ◽  
Exon 2 ◽  
Mediterranean River

This Research Communication describes the association between genetic variation within the prolactin (PRL) gene and the milk production traits of Italian Mediterranean river buffalo (Bufala mediterranea Italiana). High resolution melting (HRM) techniques were developed for genotyping 465 buffaloes. The association of genetic polymorphism with milk production traits was performed and subsequently the effects of parity and calving season were evaluated. Single nucleotide polymorphisms (SNPs) were identified at exons 2 and 5 and at introns 1 and 2. All the SNPs were in Hardy–Weinberg equilibrium, and statistical analysis showed that the polymorphism of intron1 was significantly (P < 0·05) associated with milk yield, milk protein content and peak milk yield. The average contribution of the intron1 genotype (r2intron1) to total phenotypic variance in milk production traits was 0·09, and the TT genotype showed lower values than CC and CT genotypes. A nonsynonymous SNP was identified in exon 2, which resulted in an amino acid change from arginine to cysteine. Moreover, the polymorphism of exon 2 was associated significantly with milk fat content (P < 0·05), and the buffaloes with TT genotype showed higher total fat content than the buffaloes with CT genotype. These findings provide evidence that polymorphisms of the buffalo PRL gene are associated with milk production traits and PRL can be used as a candidate gene for marker-assisted selection in Italian Mediterranean river buffalo breeding.

Investigation of myostatin and calpain 3 gene polymorphisms and their association with milk-production traits in Sfakia sheep

2020 ◽  
Vol 60 (3) ◽  
pp. 347
Author(s):  
Adel H. M. Ibrahim ◽  
Nikolaos Tzanidakis ◽  
Smaragda Sotiraki ◽  
Huitong Zhou ◽  
Jonathan Hickford
Keyword(s):  
Nucleotide Sequence ◽  
Milk Production ◽  
Milk Yield ◽  
Milk Fat ◽  
Fat Content ◽  
Solid Content ◽  
Production Traits ◽  
Milk Production Traits ◽  
Banding Patterns ◽  
Milk Solid

Context Genetic selection based on genetic markers for economically important traits in Sfakia sheep. Aims The aim of the present study was to investigate variation in the ovine myostatin gene (MSTN) and calpain 3 gene (CAPN3), and their association with milk-production traits. Methods Records for milk yield, milk fat content, protein content, lactose content, and non-fat solid content, pH and somatic-cell score (log), were obtained from 376 Sfakia ewes. Polymerase chain reaction–single-strand conformational polymorphism (PCR–SSCP) analyses were used to detect variation in intron 1 of MSTN and exon 10 of CAPN3. General linear models were then used to test for associations between the variation in MSTN and CAPN3, and milk-production traits. Key results The SSCP banding patterns for MSTN showed four variants (A1, A2, A3 and A4), which contained nine nucleotide sequence differences. Four SSCP banding patterns (C1, C2, C3 and C4) were observed for CAPN3 and these contained eight nucleotide-sequence differences. The MSTN variation was associated (P &lt; 0.05) with variation in milk yield and non-fat milk solid content. Variation in CAPN3 was associated with milk yield (P &lt; 0.001), fat content (P &lt; 0.05) and lactose content (P &lt; 0.05). Association analyses between the presence/absence of MSTN and CAPN3 variants and milk-production traits showed that a variant of MSTN that had previously between associated with muscle hypertrophy was associated with decreased milk yield (P &lt; 0.05) and a lower non-fat milk solid content (P &lt; 0.01). A CAPN3 variant that had previously been associated with increased sheep-carcass loin lean-meat yield was associated with a decreased milk yield (P &lt; 0.01) and a decreased milk fat content (P &lt; 0.05). Conclusions Our results have provided an insight into the effects of variation in ovine MSTN and CAPN3 on milk-production traits in sheep. Implications To preserve the dual-purpose characteristics of Sfakia sheep, breeding goals should take into account the possible antagonism between meat and milk traits.

The effect of physiological state, milk production traits and environmental conditions on milk fat globule size in cow's milk

2019 ◽  
Vol 86 (4) ◽  
pp. 454-460 ◽  
Author(s):  
Leonie Walter ◽  
Sue Finch ◽  
Brendan Cullen ◽  
Richard Fry ◽  
Amy Logan ◽  
...  
Keyword(s):  
Milk Production ◽  
Milk Yield ◽  
Environmental Conditions ◽  
Milk Fat ◽  
Physiological State ◽  
Production Traits ◽  
Milk Production Traits ◽  
Milk Fat Globule ◽  
Fat Globule ◽  
On Farm

AbstractThis research was carried out to quantify the effects of a range of variables on milk fat globule (MFG) size for a herd of Holstein-Friesian cows managed through an automatic milking system with year-round calving. We hypothesised that the overall variation in average MFG size observed between individual animals of the same herd cannot sufficiently be explained by the magnitude of the effects of variables that could be manipulated on-farm. Hence, we aimed to conduct an extensive analysis of possible determinants of MFG size, including physiological characteristics (parity, days in milk, days pregnant, weight, age, rumination minutes, somatic cell count) and milk production traits (number of milkings, milk yield, fat yield, protein and fat content, fat-protein ratio) on the individual animal level; and environmental conditions (diet, weather, season) for the whole herd. Our results show that when analysed in isolation, many of the studied variables have a detectable effect on MFG size. However, analysis of their additive effects identified days in milk, parity and milk yield as the most important variables. In accordance with our hypothesis, the estimated effects of these variables, calculated using a multiple variable linear mixed model, do not sufficiently explain the overall variation between cows, ranging from 2.70 to 5.69 µm in average MFG size. We further show that environmental variables, such as sampling day (across seasons) or the proportion of pasture and silage in the diet, have limited effects on MFG size and that physiological differences outweigh the effects of milk production traits and environmental conditions. This presents further evidence that the selection of individual animals is more important than the adjustment of on-farm variables to control MFG size.

Effect of DGAT1 and TG gene polymorphisms on intramuscular fat and on milk production traits in different cattle breeds in Hungary

2008 ◽  
Vol 56 (2) ◽  
pp. 181-186 ◽  
Author(s):  
István Anton ◽  
Katalin Kovács ◽  
László Fésüs ◽  
József Várhegyi ◽  
László Lehel ◽  
...  
Keyword(s):  
Milk Production ◽  
Milk Fat ◽  
Longissimus Dorsi ◽  
Production Traits ◽  
Milk Production Traits ◽  
Fat Percentage ◽  
Longissimus Dorsi Muscle ◽  
Cattle Breeds ◽  
Pcr Rflp ◽  
The Difference

The objective of this study was to estimate the effect of the thyroglobulin (TG) locus on beef quality traits in some beef cattle breeds and to investigate the effect of the DGAT1 locus on milk production traits in the Hungarian Holstein Friesian population. TG and DGAT1 genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. At the TG locus TT bulls showed the highest fat percentage values in the longissimus dorsi muscle (m. longissimus dorsi); the difference between CC and TT genotypes was significant. DGAT1 GC/GC cows had the highest milk, fat and protein yield values. Due to the relatively small number of GC/GC cows the difference proved to be significant only between AA/AA and AA/GC genotypes.

Association of bovineCD4andSTAT5bsingle nucleotide polymorphisms with somatic cell scores and milk production traits in Chinese Holsteins

2011 ◽  
Vol 78 (2) ◽  
pp. 242-249 ◽  
Author(s):  
Yanghua He ◽  
Qin Chu ◽  
Peipei Ma ◽  
Yachun Wang ◽  
Qin Zhang ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Somatic Cell ◽  
Milk Production ◽  
Milk Yield ◽  
Milk Fat ◽  
Protein Yield ◽  
Nucleotide Polymorphisms ◽  
Production Traits ◽  
Milk Production Traits ◽  
Chinese Holstein

CD4+T cells play a key role in the immune response of pathogen-induced mastitis in dairy cattle. Mammary gland factor STAT5b is involved in the regulation of CD4+T cell differentiation during inflammatory response and milk production. Little is known about the genetic variation effects of bovineCD4andSTAT5bgenes on somatic cell score (SCS) and milk production traits in dairy cattle. The aim of the study was to investigate the single nucleotide polymorphisms (SNPs) of bovineCD4andSTAT5bin Chinese Holsteins and to analyse their association with estimated breeding values (EBVs) for SCS and milk production traits. In the present study, SNPs ofCD4(NC_007303 g.13598C>T) andSTAT5b(NC_007317 g.31562 T>C) were identified and genotyped in Chinese Holstein population. The results showed that both SNPs were significantly associated with the EBVs for milk yield and protein yield in Chinese Holstein cows, and the SNP inCD4was associated with the EBV for SCS (P<0·01). The additive effect ofCD4SNP on protein yield was significant (P<0·05), and the dominant effect ofSTAT5bSNP was significant on milk yield and protein yield (P<0·01). Cows with combination genotype C7 (CCTT:CD4g.13598C>T andSTAT5bg.31562 T>C) had the highest SCS EBV but lower milk yield, while cows with C2 (TTTC) produced more milk, fat and protein than the other eight combination genotypes. These results suggested that the SNPs inCD4andSTAT5bmay be potential genetic markers for SCS and milk/protein yields selecting and warrant further functional research.

scholarly journals Associations between <i>Leu/Val</i> polymorphism of growth hormone gene and milk production traits in Black-and-White cattle

2002 ◽  
Vol 45 (5) ◽  
pp. 421-428 ◽  
Author(s):  
A. Dybus
Keyword(s):  
Growth Hormone ◽  
Milk Production ◽  
Milk Fat ◽  
Growth Hormone Gene ◽  
Production Traits ◽  
Milk Production Traits ◽  
Black And White ◽  
Holstein Friesian ◽  
Pcr Rflp ◽  
White Cattle

Abstract. Associations between polymorphism of the bovine growth hormone (GH) gene (Leu/Val) and milk production traits of Black-and-White cattle with different proportional share of Holstein Friesian genes were analysed. A total of 1086 cows were included in the study. PCR-RFLP method was used for genotyping. The frequencies of genotypes and alleles were as follows: 0.653 for LL genotype, 0.324 for LV and 0.023 for VV, and 0.815 for GHL and 0.185 for GHV. Associations between Leu/Val polymorphism and milk production traits of cows were found only in first lactation. Cows with LL genotype had higher milk, fat and protein yield compared to LV individuals (P ≤ 0.01).

scholarly journals Effect of C-CSN and B-CSN genotypes on milk production traits in Czech Flekvieh and Holstein breed

2007 ◽  
Vol 55 (1) ◽  
pp. 55-58
Author(s):  
Veronika Gazdová ◽  
Petr Humpolíček ◽  
Vanda Déduchová ◽  
Jitka Filkuková ◽  
Josef Dvořák
Keyword(s):  
Protein Content ◽  
Linear Model ◽  
Milk Production ◽  
Milk Yield ◽  
Mixed Linear Model ◽  
Agarose Gel ◽  
Production Traits ◽  
Milk Production Traits ◽  
Holstein Breed ◽  
Pcr Rflp

The effect of the χ-casein (C-CSN) and β –casein (B-CSN) loci on the milk production traits (milk yield, fat, protein and lactose content) was estimated for 807 and 488 Czech Fleckvieh and 402 and 244 Holstein cows, respectively. Genotypes of C-CNS and B-CNS were determined by the use of PCR–RFLP method. The genotypes were detected by use of electrophoresis on agarose gel. The associations of studied polymorphisms with milk production traits were estimated using the mixed linear model procedure REML in SAS for Windows 9.1.3. Results indicated that protein content is significantly affected (P ≤ 0.01) by C-CSN genotype (genotype BB > AB > AA). Fat and lactose content were not affected by C-CSN locus. The B-CNS locus had no significant effect on any milk production traits.

scholarly journals The association of five polymorphisms with milk production traits in Czech Fleckvieh cattle

2012 ◽  
Vol 57 (No. 2) ◽  
pp. 45-53 ◽  
Author(s):  
J. Boleckova ◽  
J. Matejickova ◽  
M. Stipkova ◽  
J. Kyselova ◽  
L. Barton
Keyword(s):  
Linkage Disequilibrium ◽  
Milk Production ◽  
Milk Yield ◽  
Milk Fat ◽  
Milk Protein ◽  
Future Research ◽  
Production Traits ◽  
Milk Production Traits ◽  
Breeding Values ◽  
Prl Gene

The aim of this study was to estimate allelic and genotypic frequencies of five DNA markers that are positional and functional candidates for milk production traits in Czech Fleckvieh cattle. In addition, we evaluated the association of these markers with milk production traits and breeding values for milk production traits and also estimated linkage disequilibrium (LD) between two markers within the prolactin (PRL) gene. As part of this study, 505 Czech Fleckvieh cows were genotyped. The markers in proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A), secreted phosphoprotein (SPP1), cytochrome P450 family 11 subfamily B hydroxylase (CYP11B1), and the two polymorphisms in the prolactin gene (PRL) showed evidence of segregation in our study. The PPARGC1A polymorphism was associated with milk yield, milk fat and protein traits. The polymorphism in SPP1 was significantly associated with milk protein percentage. The CYP11B1 polymorphism showed positive associations with milk composition traits and breeding values for milk yield, milk fat, and protein traits. Both polymorphisms within the PRL gene were associated with milk yield, milk fat and milk protein yield (individually and grouped). Linkage disequilibrium between the two polymorphisms in PRL was not observed. In conclusion, all markers examined in this study are important markers for milk production traits in Czech Fleckvieh cattle, and both markers within the PRL gene should be evaluated in future research. &nbsp;

scholarly journals Effect of <i>DGAT1, leptin</i> and <i>TG</i> gene polymorphisms on some milk production traits in different dairy cattle breeds in Hungary

2012 ◽  
Vol 55 (4) ◽  
pp. 307-314 ◽  
Author(s):  
I. Anton ◽  
K. Kovács ◽  
G. Holló ◽  
V. Farkas ◽  
F. Szabó ◽  
...  
Keyword(s):  
Milk Yield ◽  
Milk Fat ◽  
Milk Protein ◽  
Fragment Length Polymorphism ◽  
Production Traits ◽  
Milk Production Traits ◽  
Holstein Friesian ◽  
Pcr Rflp ◽  
Yield Values ◽  
Polymerase Chain

Abstract. The objective of this study was to estimate the effect of acylCoA-diacylglycerol-acyltransferase 1 (DGAT1), leptin and thyroglobulin (TG) loci on the milk fat, milk protein and milk yield in Holstein Friesian, Jersey and Hungarian Simmental cows. Leptin and DGAT1 genotypes were determined by qPCR assay, while TG genotypes were identified using the polymerase chain reaction-restriction fragment length polymorphism technique (PCR-RFLP). DGAT1 GC/GC cows had the highest 305-day milk yield values. Difference between AA/AA and GC/GC genotypes was significant (P<0.05). Leptin CC animals produced significantly higher 305-day milk protein percent values (P<0.05) than other genotypes in Hungarian Simmental breed. At TG locus TT cows showed the highest 305-day milk fat percent values, although differences between genotypes proved to be significant (P<0.05) only in Jersey breed.

scholarly journals Preliminary Research Concerning the Association Between the Genotypes at the Kappa-Caseine Locus and Milk Production Traits in Cattle

2001 ◽  
pp. 45-48
Author(s):  
Augustin Vlaic ◽  
Daniel Ciobanu ◽  
Teofil Oroian
Keyword(s):  
Milk Production ◽  
Milk Fat ◽  
Pcr Amplification ◽  
Production Traits ◽  
Milk Production Traits ◽  
Hair Roots ◽  
Frozen Semen ◽  
Pcr Rflp ◽  
Preliminary Research

The genotypes for kappa-casein locus were established using PCR-RFLP. Genome DNA for PCR amplification was isolated from hair roots in cows (30 Schwitz individuals), and from frozen semen in bulls (42 bulls of different breeds).A higher frequency of the B allele in Schwitz (pA = 0.45 and qB = 0.55 in cows and pA = 0.25 and qB = 0.75 in bulls), and a higher frequency of the A allele in bulls belonging to different breeds (pA = 0.61 and qB = 0.39 in Deutsch Simmental, pA = 0.84 and qB = 0.16 in Romanian Simmental; pA = 1.00 and qB = 0.00 in Holstein, and pA = 0.67 and qB = 0.33 in Pinzgauer) were estimated. The associations between the genotypes and alleles determined at the kappa-casein locus and milk production were tested. They emphasized significant associations only for milk and milk fat quantity, in advantage of individual carriers of the B allele in homozygous and heterozygous states.

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