Making electro-competent cells v1

protocols.io ◽  
2021 ◽  
Author(s):  
Ashwinuday not provided
Keyword(s):  
Immunobiology ◽  
2020 ◽  
Vol 225 (2) ◽  
pp. 151892 ◽  
Author(s):  
P. Cruz ◽  
E. Sosoniuk-Roche ◽  
I. Maldonado ◽  
C.G. Torres ◽  
A. Ferreira

2019 ◽  
Vol 2 (1) ◽  

The present paper addresses anatomically resolved protein networks by using the Imaging Cycler Microscopy (ICM/TIS) [1,2]. ICM is capable of resolving protein networks in intact anatomical structures at a power of combinatorial molecular resolution of 65,553k , where k is the number of co-mapped proteins, e.g. 100 proteins [1-4]. This method provides insight into the laws of the spatial communication of large protein networks in health and disease, which is essential for new therapy options in diseases.


1979 ◽  
Vol 33 (1) ◽  
pp. 1-14 ◽  
Author(s):  
L. O. Butler ◽  
G. Nicholas ◽  
R. W. Grist

SUMMARYTransformation studies in pneumococcus had shown that loci determining the resistance to erythromycin and streptomycin were unlinked when strain Cl3 was recipient but linked when strain SIII-I was recipient. This phenomenon also applies to other pairs of markers studied in these two recipients, no matter whether the transforming DNA was derived from strain Cl3 or strain SIII-I. Other differences between the two recipient strains were also revealed. Whereas competent cultures of strain Cl3 were composed of all competent cells, which was in agreement with previous reports of pneumococcal cultures, strain SIII-I normally gave a maximum average of 28% competent cells. Strain SIII-I was unstable, since on repeated sub-culturing the competence peak profile changed and the value of ‘fcq’ increased. These properties were reflected in the twohex−strains 401 and R6x which were found to be similar to the ‘altered SIII-I’ strain. The results from the linkage studies have been applied to the chromosome map and have placed theopt-r2gene in thestr-r41-containing chromosome arm.


2004 ◽  
Vol 26 (20) ◽  
pp. 1593-1594 ◽  
Author(s):  
Taeho Ahn ◽  
Seong Su Kang ◽  
Chul-Ho Yun

PEDIATRICS ◽  
1967 ◽  
Vol 40 (3) ◽  
pp. 469-470
Author(s):  
REBECCA H. BUCKLEY

Dr. Miller's point (suggested by the work of Kadowaki, et al.) is well taken that immunologically competent cells of maternal origin could likely survive in infants with thymic alymphoplasia and perhaps function as immunoglobulin-producing cells in the infant. We did not touch upon this point in our discussion because we had no karyotyping data on our patient and also because there was nothing present clinically to suggest a graft vs host reaction. In the case of Kadowaki, et al., the patient failed to grow after 3 months of age and was notably "runted."


2019 ◽  
Vol 167 (2) ◽  
pp. 229-232 ◽  
Author(s):  
G. N. Zyuz’kov ◽  
L. A. Miroshnichenko ◽  
T. Yu. Polyakova ◽  
L. A. Stavrova ◽  
E. V. Simanina ◽  
...  

1985 ◽  
Vol 198 (2) ◽  
pp. 329-335 ◽  
Author(s):  
Charles W. Finn ◽  
Otto E. Landman

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