IDENTIFICATION OF S-ALLELES IN 25 SWEET CHERRY (PRUNUS AVIUM L.) CULTIVARS BY ALLELE-SPECIFIC PCR AMPLIFICATION

2009 ◽  
pp. 619-622 ◽  
Author(s):  
A. Ershadi ◽  
E.H. Moghadam
Keyword(s):  
Sweet Cherry ◽  
Prunus Avium ◽  
Pcr Amplification ◽  
Specific Pcr ◽  
S Alleles ◽  
Allele Specific ◽  
Allele Specific Pcr ◽  
Prunus Avium L

scholarly journals Self-Incompatibility Alleles in Iranian Pear Cultivars

2019 ◽  
Author(s):  
Maryam Bagheri ◽  
Ahmad Ershadi
Keyword(s):  
Rapid Method ◽  
Pcr Amplification ◽  
Self Incompatibility ◽  
Specific Primers ◽  
Specific Pcr ◽  
S Alleles ◽  
Allele Specific ◽  
Incompatibility Alleles ◽  
Consensus Primers ◽  
Allele Specific Pcr

AbstractIn the present study, the S-alleles of eighteen pear cultivars, (including fourteen cultivars planted commercially in Iran and four controls) are determined. 34 out of 36 S-alleles are detected using nine allele-specific primers, which are designed for amplification of S101/S102, S105, S106, S107, S108, S109, S111, S112 and S114, as well as consensus primers, PycomC1F and PycomC5R. S104, S101 and S105 were the most common S-alleles observed, respectively, in eight, seven and six cultivars. In 16 cultivars, (‘Bartlett’ (S101S102), ‘Beurre Giffard’ (S101S106), ‘Comice’ (S104S105), ‘Doshes’ (S104S107), ‘Koshia’ (S104S108), ‘Paskolmar’ (S101S105), ‘Felestini’ (S101S107), ‘Domkaj’ (S104S120), ‘Ghousi’ (S104S107), ‘Kaftar Bache’ (S104S120), ‘Konjoni’ (S104S108), ‘Laleh’ (S105S108), ‘Natanzi’ (S104S105), ‘Sebri’ (S101S104), ‘Se Fasleh’ (S101S105) and ‘Louise Bonne’ (S101S108)) both alleles are identified but in two cultivars, (‘Pighambari’ (S105) and ‘Shah Miveh Esfahan’ (S107)) only one allele is recognized. It is concluded that allele-specific PCR amplification can be considered as an efficient and rapid method to identify S-genotype of Iranian pear cultivars.

Simple and Rapid Detection of Factor V Leiden by Allele-specific PCR Amplification

1996 ◽  
Vol 75 (05) ◽  
pp. 757-759 ◽  
Author(s):  
Rainer Blasczyk ◽  
Markus Ritter ◽  
Christian Thiede ◽  
Jenny Wehling ◽  
Günter Hintz ◽  
...  
Keyword(s):  
Direct Sequencing ◽  
Activated Protein C ◽  
Factor V Leiden ◽  
Pcr Amplification ◽  
Factor V ◽  
Specific Pcr ◽  
Pcr Rflp ◽  
Allele Specific ◽  
Specific Amplification ◽  
Allele Specific Pcr

SummaryResistance to activated protein C is the most common hereditary cause for thrombosis and significantly linked to factor V Leiden. In this study, primers were designed to identify the factor V mutation by allele-specific PCR amplification. 126 patients with thromboembolic events were analysed using this technique, PCR-RFLP and direct sequencing. The concordance between these techniques was 100%. In 27 patients a heterozygous factor VGln506 mutation was detected, whereas one patient with recurrent thromboembolism was homozygous for the point mutation. Due to its time- and cost-saving features allele-specific amplification should be considered for screening of factor VGln506.

scholarly journals Detection of maternal cells in human fetal blood during the third trimester of pregnancy using allele‐specific PCR amplification

1997 ◽  
Vol 98 (3) ◽  
pp. 767-771 ◽  
Author(s):  
THIERRY PETIT ◽  
MARC DOMMERGUES ◽  
GÉRARD SOCIÉ ◽  
YVES DUMEZ ◽  
ELIANE GLUCKMAN ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
Fetal Blood ◽  
Third Trimester ◽  
The Third ◽  
Specific Pcr ◽  
Allele Specific ◽  
Allele Specific Pcr

scholarly journals Wild and Rare Self-Incompatibility Allele S17 Found in 24 Sweet Cherry (Prunus avium L.) Cultivars

2021 ◽  
Author(s):  
Agnes Kivistik ◽  
Liina Jakobson ◽  
Kersti Kahu ◽  
Kristiina Laanemets
Keyword(s):  
Sweet Cherry ◽  
Prunus Avium ◽  
Eastern European ◽  
S Locus ◽  
Sweet Cherries ◽  
S Alleles ◽  
Allele Specific ◽  
S Allele ◽  
Sweet Cherry Cultivars ◽  
Consensus Primers

AbstractThe pollination of self-incompatible diploid sweet cherry is determined by the S-locus alleles. We resolved the S-alleles of 50 sweet cherry cultivars grown in Estonia and determined their incompatibility groups, which were previously unknown for most of the tested cultivars. We used consensus primers SI-19/20, SI-31/32, PaConsI, and PaConsII followed by allele-specific primers and sequencing to identify sweet cherry S-genotypes. Surprisingly, 48% (24/50) of the tested cultivars, including 17 Estonian cultivars, carry the rare S-allele S17, which had initially been described in wild sweet cherries in Belgium and Germany. The S17-allele in Estonian cultivars could originate from ‘Leningradskaya tchernaya’ (S6|S17), which has been extensively used in Estonian sweet cherry breeding. Four studied cultivars carrying S17 are partly self-compatible, whereas the other 20 cultivars with S17 have not been reported to be self-compatible. The recommended pollinator of seven self-incompatible sweet cherries is of the same S-genotype, including four with S17-allele, suggesting heritable reduced effectiveness of self-infertility. We classified the newly genotyped sweet cherry cultivars into 15 known incompatibility groups, and we proposed four new incompatibility groups, 64–67, for S-locus genotypes S3|S17, S4|S17, S5|S17, and S6|S17, respectively, which makes them excellent pollinators all across Europe. Alternatively, the frequency of S17 might be underestimated in Eastern European populations and some currently unidentified sweet cherry S-alleles might potentially be S17.

scholarly journals Update on and Review of the Incompatibility (S-) Genotypes of Apple Cultivars

HortScience ◽  
2004 ◽  
Vol 39 (5) ◽  
pp. 943-947 ◽  
Author(s):  
Wim Broothaerts ◽  
Ilse Van Nerum ◽  
Johan Keulemans
Keyword(s):  
Identification System ◽  
Published Data ◽  
Specific Pcr ◽  
S Alleles ◽  
Allele Specific ◽  
Self Fertilization ◽  
Apple Cultivars ◽  
S Allele ◽  
Allele Specific Pcr

Apple cultivars display a self-incompatibility system that restricts self-fertilization and fertilization between cultivars bearing identical S-alleles. There has been considerable progress in identification of S-alleles in apple in recent years and methods are now available for the accurate S-genotyping of cultivars. Following a recently revised numerical identification system for apple S-alleles, we present the first extensive compilation of apple cultivars with their S-genotypes. This list contains data from our own investigations using S-allele-specific PCR methodology, including a number of new data, as well as published data from various other sources. Eighteen different S-alleles are discriminated, which allowed the determination of the S-genotypes for 150 diploid or triploid European, American, and Japanese cultivars. Many of these cultivars are cultivated worldwide for their fruit. Also included are a number of old, obsolete cultivars and a few nondomestic genotypes. We observed a wide variation in the frequency of S-alleles in the apple germplasm. Three S-alleles (S2, S3, and S9) are very common in the cultivars evaluated, presumably as a result of the widespread use of the same breeding parents, and seven alleles are very rare (S4, S6, S8, S16, S22, S23, S26).

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