Production of doubled haploid plants of Cucurbitaceae family crops through unpollinated ovule culture in vitro

2020 ◽  
pp. 19-28
Author(s):  
E. Domblides ◽  
N. Shmykova ◽  
G. Khimich ◽  
I. Korotseva ◽  
L. Kan ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Ovule Culture ◽  
Culture In Vitro ◽  
Doubled Haploid Plants ◽  
Cucurbitaceae Family ◽  
Haploid Plants

scholarly journals RNA‐guided endonuclease‐driven mutagenesis in tobacco followed by efficient fixation of mutated sequences in doubled haploid plants

2016 ◽  
Author(s):  
Sindy Schedel ◽  
Stefanie Pencs ◽  
Goetz Hensel ◽  
Andrea Mueller ◽  
Jochen Kumlehn
Keyword(s):  
Doubled Haploid ◽  
Fluorescent Protein ◽  
Leaf Explants ◽  
Site Directed Mutagenesis ◽  
Green Fluorescent Protein Gene ◽  
Vegetative Plant ◽  
Embryogenic Pollen ◽  
Doubled Haploid Plants ◽  
Haploid Plants

Background: Customizable endonucleases are providing an effective tool for genome engineering. The resulting primary transgenic individuals are typically heterozygous and/or chimeric with respect to any mutations induced. To generate genetically fixed mutants, they are conventionally allowed to self-pollinate, a procedure which segregates individuals into mutant heterozygotes/homozygotes and wild types. The chances of recovering homozygous mutants among the progeny depends not only on meiotic segregation but also on the frequency of mutated germline cells in the chimeric mother plant. Results: RNA-guided endonuclease-mediated mutagenesis was targeted to the green fluorescent protein gene (gfp) harboured by a transgenic tobacco line. Upon retransformation using agfp-specific endonuclease construct, the T0plants were allowed to either self-pollinate, or were propagated via regeneration fromin vitrocultured embryogenic pollen which give rise to haploid/doubled haploid plants or from leaf explants that form plants vegetatively. Single or multiple mutations were detected in 80% of the T0plants. The majority of these mutations proved heritable by each of the three propagation systems used. Regeneration fromin vitrocultured embryogenic pollen allowed for homozygous mutants to be produced more efficiently than via sexual reproduction. The recovery of mutations that were not found among sexually produced progeny was shown to be achievable through vegetative plant propagationin vitro. In addition, a number of mutations not detected in the primary gRNA/Cas9-expressing plants were uncovered in the progeny, irrespective of the mode of propagation. Conclusion: Regeneration from embryogenic pollen culture provides a convenient method to rapidly generate a variety of genetically fixed mutants following site-directed mutagenesis. Induced mutations that are not sexually transmitted can be recovered through vegetative plant regeneration from somatic tissue.

scholarly journals PROSPECTIVE OF DEVELOPMENT OF DOUBLED HAPLOID PLANTS OF CUCURBITACEAE FAMILY

2015 ◽  
pp. 28-31 ◽  
Author(s):  
N. A. Shmykova ◽  
◽  
G.A. Khimich ◽  
I. B. Korotseva ◽  
E. A. Domblides ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Doubled Haploid Plants ◽  
Cucurbitaceae Family ◽  
Haploid Plants

Regeneration of Doubled Haploid Plants from in vitro Selected Microspores to improve Aluminium Tolerance in Wheat

2000 ◽  
Vol 156 (2) ◽  
pp. 217-222 ◽  
Author(s):  
Beäta Barnabäs ◽  
Gezä Koväcs ◽  
Attlla Hegedűs ◽  
Sära Erdei ◽  
Gäbor Horväth
Keyword(s):  
Doubled Haploid ◽  
Aluminium Tolerance ◽  
Doubled Haploid Plants ◽  
Haploid Plants

scholarly journals Regenerasi dan Aklimatisasi Kultur Antera Enam Persilangan F1 Padi Sawah

2016 ◽  
Vol 44 (2) ◽  
pp. 133
Author(s):  
Cucu Gunarsih ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Dan Muhamad Syukur
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Callus Induction ◽  
Rainfed Rice ◽  
Randomized Design ◽  
Doubled Haploid Plants ◽  
Completely Randomized Design ◽  
N6 Medium ◽  
Haploid Plants

ABSTRACT<br /><br />The breeding of rainfed rice tolerant to drought can be accomplished using anther culture. The objectives of this research were to determine regeneration abilities of six F1 anther culture and its acclimatization ability. The experiment was arranged in completely randomized design with 14 replications. The treatments consisted of six F1 derived from crossing:  INPARI 18 x IR83140-B-11-B (G1), INPARI 18 x B12825E-TB-1-25 (G2), INPARI 18 x IR87705-14-11-B-SKI-12 (G3), INPARI 22 x IR83140-B-11-B (G4), Bio-R81 x O18b-1 (G5), Bio-R82-2 x O18b-1 (G6). Media for callus induction was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1 kinetin + 1.0 mM putresin + 60 g L-1 sucrosa, media for regeneration was based on MS + 0.5 mg L-1 NAA + 2.0 mg L-1 kinetin + 1.0 mM  putresin, and media for rooting was based on  MS + 0.5 mg L-1 IBA + 30 g L-1 sucrosa. The result indicated that all six F1 had different ability in anther culture. Bio-R82-2 x O18-b1 (G6) and  Bio-R81 x O18-b1 (G5) F1 genotype had good response both of callus induction and plant regeneration. These two F1 genotypes also gave the highest ratio of green planlet production to number of anther inoculated (GP:AI) were 5.50% and 4.65%,  respectively. In this research, there were identified doubled haploid plants were developed from 4 F1 derived cross namely G2 (2 plants), G3 (4 plants),  G5 (21 plants), and G6 (26 plants).<br /><br />Keywords: Callus induction, doubled haploid, rice<br /><br />

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