scholarly journals Oxidative modification of proteins in rat serum under experimental osteoarthrosis and long-term administration of a multiprobiotic

2019 ◽  
Vol 26 (1) ◽  
pp. 50-54
Author(s):  
A. Vovk ◽  
O. Korotkyi ◽  
L. Kot ◽  
K. Dvorshchenko
Keyword(s):  
Drinking Water ◽  
Oxidative Modification ◽  
Sulfhydryl Groups ◽  
Intragastric Administration ◽  
Nacl Solution ◽  
Rat Serum ◽  
Knee Ligament ◽  
Oxidative Modification Of Proteins ◽  
Term Administration

The aim of the study was to investigate the effect of multiprobiotics on the content of products of oxidative modification of proteins and the level of sulfhydryl groups in blood serum of rats during monoiodoacetate-induced osteoarthritis. The study was carried out on white non-linear, sexually mature male rats (weight 180-240 g), according to general ethical principles of experiments on animals. All animals were divided into four experimental groups. The first group - Control: animals got injection into knee ligament 0.05 ml of 0.9% NaCl solution on the first day of the experiment and then got intragastric administration 1 ml of drinking water per 1 kg of the animal weight daily for 14 days from the 8th to 22nd days. The second group - Multiprobiotic: animals got injection into knee ligament 0.05 ml of 0.9% NaCl solution on the first day of the experiment and then got intragastric administration 140 mg/kg of multiprobiotic Symbiter® (Prolisok ", Ukraine) diluted in 1 ml of drinking water per 1 kg of animal weight. The third group, MIA-induced OA: animals got injection into knee ligament 1 mg of sodium monoiodacetate, dissolved in 0.05 ml of 0.9% NaCl on the first day of the experiment and then got intragastric administration 1 ml of drinking water per 1 kg of the animal weight daily for 14 days from the 8th to 22nd days. The fourth group – MIA-induced OA + Multiprobiotic: animals got injection into knee ligament 0.05 ml of 1 mg of sodium monoiodacetate, dissolved in 0.05 ml of 0.9 % NaCl on the first day of the experiment and then got intragastric administration 140 mg/kg of multiprobiotic diluted in 1 ml of drinking water per 1 kg of animal weight. All animals were killed on day 30 of the experiment, according to the protocol of the ethics committee with rapid blood sampling. The content of the products of oxidative modification of proteins (OMP) and oligopeptides was determined by the level of carbonyl derivatives that were detected in reaction with 2,4-initrophenylhydrazine. The level of total, protein-bound and non-protein sulfhydryl (SH) -groups was measured by the Elman method. It has been established that MIA-induced OA disturbed oxidative-antioxidant balance of the rat serum: the content of the products of oxidative modification of proteins increases and the content of sulfhydryl groups decreases in the serum. It was shown that with the long-term administration of multiprobiotics in animals with MIA-induced OA, the above indicators were restored.

scholarly journals Lipid peroxidation in rat cartilage under experimental osteoarthritis and administration of multiprobiotic

2020 ◽  
Vol 80 (1) ◽  
pp. 41-44
Author(s):  
O. Korotkyi ◽  
L. Kot ◽  
K. Dvorshchenko
Keyword(s):  
Lipid Peroxidation ◽  
Drinking Water ◽  
Schiff Bases ◽  
Male Rats ◽  
Intragastric Administration ◽  
Nacl Solution ◽  
Diene Conjugates ◽  
Active Compounds ◽  
Knee Ligament ◽  
Lipid Peroxidation Products

The aim of the study was to investigate the effect of multiprobiotic on the content of lipid peroxidation products in rat cartilage during monoiodoacetate-induced osteoarthritis. The study was carried out on white non-linear, sexually mature male rats (weight 180-240g), according to general ethical principles of experiments on animals. All animals were divided into four experimental groups. The first group – Control: animals got injection into knee ligament 0.05 ml of 0.9% NaCl solution on the first day of the experiment and then got intragastric administration 1 ml of drinking water per 1 kg of the animal weight daily for 14 days from the 8th to 22nd days. The second group – Multiprobiotic: animals got injection into knee ligament 0.05 ml of 0.9% NaCl solution on the first day of the experiment and then got intragastric administration 140 mg / kg of multiprobiotic Symbiter® (Prolisok ", Ukraine) diluted in 1 ml of drinking water per 1 kg of animal weight. The third group, MIA-induced OA: animals got injection into knee ligament 1 mg of sodium monoiodacetate, dissolved in 0.05 ml of 0.9% NaCl on the first day of the experiment and then got intragastric administration 1 ml of drinking water per 1 kg of the animal weight daily for 14 days from the 8th to 22nd days. The fourth group – MIA-induced OA + Multiprobiotic: animals got injection into knee ligament 0.05 ml of 1 mg of sodium monoiodacetate, dissolved in 0.05 ml of 0.9% NaCl on the first day of the experiment and then got intragastric administration 140 mg / kg of multiprobiotic diluted in 1 ml of drinking water per 1 kg of animal weight. All animals were killed on day 30 of the experiment, according to the protocol of the ethics committee with rapid blood sampling. The content of the products of oxidative modification of proteins (OMP) and oligopeptides was determined by the level of carbonyl derivatives that were detected in reaction with 2,4-dinitrophenylhydrazine. The content of diene conjugates was determined in the heptane-isopropanol extract by the spectrophotometric method, and of Schiff bases – by the fluorimetric method. The content of TBK-active compounds was determined by reaction with thiobarbituric acid. It has been established that MIA-induced OA the content of lipid peroxidation products (diene conjugates, TBK-active compounds, schiff bases) increases in the cartilage. It was shown that with the administration of multiprobiotic in animals with MIA-induced OA, the above indicators were restored.

scholarly journals Oxidative modification of proteins in rat serum under experimental osteoarthritis and joint administration of a chondroprotector and multiprobiotic

2020 ◽  
Vol 81 (2) ◽  
pp. 64-68
Author(s):  
O. Korotkyi ◽  
L. Kot ◽  
K. Dvorshchenko ◽  
L. Ostapchenko
Keyword(s):  
Blood Serum ◽  
Chondroitin Sulfate ◽  
Oxidative Modification ◽  
Sulfhydryl Groups ◽  
Important Place ◽  
Knee Ligament ◽  
Oxidative Modification Of Proteins ◽  
Sh Groups ◽  
Oxidative Protein Modification ◽  
Experimental Osteoarthritis

One of the actual problems of modern medicine is joint disease. Among them, osteoarthritis occupies an important place. The formation of osteoarthritis is accompanied by the development of inflammation, which leads to damage to all structures of the joint. An important role in inflammatory processes is played by the intensification of free radical processes. As the disease develops, the joints lose their mobility, which leads to a decrease in the quality of life of patients and the development of disability. In this regard, it is important to search for drugs that have regenerative, anti-inflammatory and antiradical properties. The aim of our study was to investigate the combined effect of chondroitin sulfate and multiprobiotic on the content of oxidative protein modification products and the level of sulfhydryl groups in rat blood serum under conditions of monoiodoacetate-induced osteoarthritis. The study included participation of white male non-linear rats (weighing 180–240 g) adherence to the general ethical principles of animal experiments. An experimental osteoarthritis model was created by introducing 1 mg of sodium monoiodoacetate into the knee ligament. Chondroitin sulfate and multiprobiotic were used as therapeutic agents. The content of products of oxidative modification of proteins was determined by the level of carbonyl derivatives, which are manifested in the reaction with 2,4-dinitrophenylhydrazine. The level of total, protein-bound and non-protein sulfhydryl groups was measured by the Elman method. It was found that under conditions of monoiodoacetate-induced osteoarthritis in the blood serum of rats, the content of products of oxidative modification of proteins increases. The level of neutral aldehyde products (E max = 356 nm) is increased by 2.5 times and neutral ketone products (E max = 370 nm), respectively, by 2,1 times compared to the control. Under the same experimental conditions in the blood serum, the amount of basic aldehyde products (E max = 430 nm) increases by 1.9 times, while the content of the main ketone products (E max = 530 nm) increases by 1,7 times compared to the control groups. In experimental osteoarthritis in the blood serum, the content of sulfhydryl groups decreases: non-protein SH-groups – 1,5 times, protein and general SH-groups – 1,7 times relative to the control. This indicates disturbance of the oxidative-antioxidant balance and the development of oxidative stress in the organism during experimental osteoarthritis. It was shown that the combined administration of chondroitin sulfate and multiprobiotics in animals with experimental osteoarthritis partially restored the above parameters.

scholarly journals Oxidative modification of proteins in serum of rats under conditions of carrageenan-induced inflammation of a back limb and prolonged prophylactic administration of chondroitin sulfate

2018 ◽  
Vol 76 (2) ◽  
pp. 62-65
Author(s):  
O. Blokhina ◽  
O. Korotkiy ◽  
L. Kot ◽  
D. Negray ◽  
K. Dvorshchenko
Keyword(s):  
Chondroitin Sulfate ◽  
Therapeutic Dose ◽  
Experimental Studies ◽  
Oxidative Modification ◽  
Sulfhydryl Groups ◽  
Male Rats ◽  
Nacl Solution ◽  
Oxidative Modification Of Proteins ◽  
Prophylactic Administration ◽  
Inflammatory Edema

The aim of the work was to investigate the preventive effect of Chondroitin Sulfate on the content of products of oxidative modification of proteins and the level of sulfhydryl groups in blood serum of rats at local inflammation of the hind limb. The studies were conducted on white non-linear, sexually mature male rats weighing 180–240 g, in compliance with the general ethical principles of experiments on animals. All animals were divided into four experimental groups. The first group – control: animals sub-planar injected 0,1 ml of 0,9 % NaCl solution into the posterior right limb. The second group – animals received a therapeutic dose of 3 mg x kg-1 chondroitin sulfate daily for 28 days daily. The third group – animals were infused intramuscularly with 0,1 ml of 0,9 % NaCl solution in the posterior right limb for 28 days and for 29 days inflammatory edema of the limb was stimulated (animals were sub-planar injected with 0,1 ml of 1 % carrageenan solution to the posterior right limb ) The fourth group – for 28 days rats were daily intramuscularly injected with a therapeutic dose of 3 mg x kg-1 chondroitin sulfate, after which on 29th day, inflammatory edema of the limb was stimulated. Animals were killed 3 hours after injection of carrageenan solution according to the protocol of the ethical committee, and then blood sampling for further research was quickly taken. The total number of animals involved in experimental studies was 40 individuals. The content of the products of oxidative modification of proteins (OMP) and oligopeptides was determined by the level of carbonyl derivatives that were detected in reaction with 2,4-dinitrophenylhydrazine. The level of total, protein-bound and non-protein sulfhydryl (SH) -groups was measured by the Elman method. It has been established that with carrageenan-induced inflammation of the posterior limb, the content of the products of oxidative modification of proteins increases and the content of sulfhydryl groups decreases in the serum. It was shown that the prophylactic administration of chondroitin sulfate based drug on animals with carrageenan-induced inflammation restored the abovementioned parameters.

The evaluation of lipid peroxidation and oxidative modification of proteins in blood serum under obesity development and the consumption of aqueous kidney beans Phaseolus vulgaris pods extract

2020 ◽  
Vol 33 (1) ◽  
pp. 38-44
Author(s):  
Alona Yurchenko ◽  
Daryna Krenytska ◽  
Olexii Savchuk ◽  
Tetiana Halenova ◽  
Natalia Raksha ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Oxidative Modification ◽  
Resistance Development ◽  
Oxidative Modification Of Proteins ◽  
Kidney Beans ◽  
Calorie Diet ◽  
Diet Control ◽  
High Calorie Diet ◽  
High Calorie

AbstractOur interest has focused on the investigation of the anti-obese potential of kidney beans (P. vulgaris) pods extract. In the course of the study, obesity development in rats was induced with high-calorie diet. Control and obese rats then have consumed with aqueous kidney beans (P. vulgaris) pods extract during 6 weeks (200 mg/kg). Results show that the long-term consumption of P. vulgaris pods extract can lead to the reduction of hyperglycemia and insulin resistance development. Furthermore, we saw a normalization of lipid peroxidation parameters and oxidative modification of protein due to the consumption of the kidney beans (P. vulgaris) pods extract. Our experimental data demonstrate the ability of the kidney beans (P. vulgaris) pod extracts to mitigate obesity development but the details of this mechanism remains to be not fully understood.

scholarly journals THE EFFECT OF LONG-TERM INTAKE OF ATORVASTATIN AND 1-CHOLECALCIFEROL ON THE PHYSICAL AND CHEMICAL PROPERTIES OF LIPOPROTEIN BLOOD COMPLEXES OF LABORATORY RATS

2017 ◽  
pp. 49-52
Author(s):  
S. S. Osuchuk ◽  
O. S. Yakovleva
Keyword(s):  
Chemical Properties ◽  
Intragastric Administration ◽  
Physical And Chemical Properties ◽  
Laboratory Rats ◽  
Combined Application ◽  
Term Administration ◽  
Physical And Chemical ◽  
Positive Effect ◽  
And Chemical Properties

Object: to study the physical and chemical properties of the lipoprotein complexes of rats` blood in the long-term administration of statins and vitamin D. Material and methods. The experiment was performed on 4 experimental groups of rats for 90 days: 1 - intact; 2 - placebo; 3 - intragastric administration of atorvastatin; 4 - intragastric administration of atorvastatin with α-calcidol. The lipoprotein blood complexes were isolated by the method of differential ultracentrifugation. The microflow and microviscosity of the lipoprotein complexes were determined using pyrene. Results. The placebo group revealed an increase of the micropolarity of the total lipid pool of VLDL and a decrease of the microviscosity of the annulary pool of LDL and HDL. The administration of atorvastatin reduces the microviscosity of HDL. The combined application of atorvastatin and α-calcidol reduces the microviscosity of HDL. Conclusion. We have drawn a conclusion about the complex effect of stress, atorvastatin, and α-calcidol on the physical and chemical properties of the lipoprotein blood complexes and a conclusion about the positive effect of atorvastatin and α-calcidol on the microviscosity of HDL.

scholarly journals The study of oxidative stress indicators in rats with a simulated acute hepatitis and correction with a thick extract from reishi mushrooms

2021 ◽  
pp. 4-9
Author(s):  
Iryna Herasymets ◽  
Liudmila Fira ◽  
Igor Medvid
Keyword(s):  
Superoxide Dismutase ◽  
Body Weight ◽  
Acute Hepatitis ◽  
The Body ◽  
Oxidative Modification ◽  
Male Rats ◽  
Intragastric Administration ◽  
Reference Drug ◽  
Oxidative Modification Of Proteins ◽  
Dry Extract

The aim. To study the effect of a dry extract from reishi mushrooms on the activity of lipoperoxidation and oxidative modification of proteins under the conditions of a simulated paracetamol hepatitis in rats. Materials and methods. The study was performed on white male rats. The animals were divided into 10 groups, each included 6 animals. Acute hepatitis was simulated by the intragastric administration of paracetamol in the dose of 1250 mg/kg once per day (for 2 days). Correction of the pathology induced was performed with a dry extract of reishi mushrooms in the dose of 100 mg/kg of the body weight. The reference drug “Silybor” was administered in the dose of 20 mg/kg of the animal body weight. On Day 3, 7 and 10 from the beginning of the lesion, rats were euthanized using sodium barbamyl. The liver homogenate and blood serum were used for the studies. The activity of free radical oxidation processes under the conditions of acute toxic hepatitis and after the introduction of corrective factors was assessed by superoxide dismutase, catalase activity, the content of TBA-AP and OMP products. Results and discussion. The development of acute paracetamol hepatitis in rats and damage of hepatocyte membranes are indicated by an increase in the content of TBA-active products, products of oxidative modification of neutral and basic proteins in the serum and liver of animals. Simultaneously, a decrease in the activity of catalase and superoxide dismutase was observed. After correction of the pathology induced with a dry extract of reishi mushrooms a significant increase in the activity of antioxidant enzymes, a decrease in the content of lipid peroxidation products and oxidative modification of proteins in the serum and liver of the affected animals were observed. Conclusions. It has been experimentally proven that the use of dry extract of Reishi mushrooms in paracetamol hepatitis in rats caused a significant decrease in catalase and superoxide dismutase activity, a decrease in TBA-AP, neutral and basic 2,4-DNPH in the serum and liver of animals. The results of the research indicate an effective impact of reishi mushrooms dry extract on the normalization of lipoperoxidation, oxidative modification of proteins and antioxidant protection

Gastric hyposecretion produced in rats by long-term oral administration of glycopyrrolate

1970 ◽  
Vol 48 (3) ◽  
pp. 157-159 ◽  
Author(s):  
K. Kowalewski ◽  
J. Schier
Keyword(s):  
Drinking Water ◽  
Oral Administration ◽  
Experimental Method ◽  
Normal Saline ◽  
Untreated Control ◽  
Anticholinergic Drug ◽  
Gastric Fistula ◽  
Secretory Function ◽  
Term Administration

An anticholinergic drug, 1-methyl-3-pyrrolidyl-2-phenylcyclopentaneglycolate methobromide (glycopyrrolate) dissolved in drinking water, was given to the rats. The treatment lasted for 5 months. A secretion study was then performed in gastric fistula-bearing animals, infused for 24 h with normal saline or histamine. A significant decrease of the secretory function of parietal and zymogenic cells was found in treated rats, as compared with untreated control animals. Long-term administration of an anticholinergic drug in drinking water appears to be an easy experimental method of induction of gastric secretory hypofunction in rats.

scholarly journals Acute and sub-acute toxicity studies of an extract from white bean pods under the condition of its intragastric administration

2016 ◽  
Vol 71 (1) ◽  
pp. 31-35
Author(s):  
M. Kuznietsova ◽  
T. Halenova ◽  
O. Savchuk
Keyword(s):  
Acute Toxicity ◽  
Intragastric Administration ◽  
Toxic Substances ◽  
Dry Extract ◽  
Acute Toxicity Study ◽  
Term Administration ◽  
White Bean ◽  
Liver And Kidney

The article is devoted to the studies of acute and sub-acute toxicity of dry extract from white bean (P. vulgaris) pods. The acute toxicity study of the extract was carried out according to the conventional classification of K.K Sidorov and showed that the extract refers to low-toxic substances (LD 50 following its intragastric administration is greater than 2000 mg/kg). The study of extract sub-acute toxicity showed the increasing of relative liver and kidney weight, which may indicate the existence of adverse effects of the extract under the conditions of its long-term administration.

Reactivity of Pulmonary Alveolar Cells to Crocidolite Asbestos Fibers

1982 ◽  
Vol 40 ◽  
pp. 334-335
Author(s):  
Charles L. Sanders ◽  
Roy R. Adee
Keyword(s):  
Osmium Tetroxide ◽  
Intratracheal Instillation ◽  
Uranyl Acetate ◽  
Fiber Suspension ◽  
Nacl Solution ◽  
Alveolar Cells ◽  
Asbestos Fibers ◽  
Fischer Rats ◽  
Mineral Silicates

Asbestos is a generic name for a group of hydrated mineral silicates that occur naturally in a fibrous form. The early interactions of asbestos fibers with alveolar cells in large part determines their long-term toxicity. Young adult, SPF, Fischer rats were given a single intratracheal instillation of 2 mg crocidolite asbestos suspended in 0.5 ml of 0.9% NaCl solution. About 80% of the fibers had lengths of less than 10 ym as measured on light micrographs of the fiber suspension. Two rats were killed at 3 hr, 1 d and 1, 4, 8, 12 and 16 wk after instillation and the lungs instilled with 8 ml McDowell - Trumps at 20 cm H2O. Lung tissue was dehydrated and sputtered coated with palladium-gold for SEM or post-fixed in osmium tetroxide, embedded in epoxy resin and sections stained with uranyl acetate and lead citrate for TEM.

The efficacy and safety of long-term administration of alpha-interferon and lamivudine in patients with chronic hepatitits B

2001 ◽  
Vol 120 (5) ◽  
pp. A572-A572
Author(s):  
F JABOLI ◽  
E RODA ◽  
C FABBRI ◽  
S MARCHETTO ◽  
F FERRARA ◽  
...  
Keyword(s):  
Alpha Interferon ◽  
Efficacy And Safety ◽  
Term Administration
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